Acta Biologica Hungarica
Volume/Issue:
Volume 57: Issue 2
Heat shock of cultivated and wild wheats during early seedling stage: growth, cell viability and heat shock proteins
Authors:
M. Yildiz Department of Biology, ANS Campus, Faculty of Science and Arts, Afyon Kocatepe University 03100 Afyonkarahisar, Turkey

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and
S. Terzioglu Department of Biology, Beytepe Campus, Faculty of Science, Hacettepe University 06532 Ankara, Turkey

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Pages:
231–246
Online Publication Date:
08 Jun 2006
Publication Date:
01 Jun 2006
Article Category:
Research Article
DOI:
https://doi.org/10.1556/abiol.57.2006.2.10
Restricted access

Growth, cell viability and heat shock proteins (HSPs) in Bezostaya-1, Çukurova-86 and Diyarbakır-81 cultivated wheat cultivars and three Aegilops species were investigated. Etiolated seedlings were exposed to 23 °C, 32 °C, 35 °C, 37 °C and 38 °C for 24 h, and 35 °C (24 h) → 50 °C(1 h) and 37 °C (24 h) → (50 °C (1 h). At the end of recovery growth periods, the shoot lengths of the genotypes generally decreased significantly at 35, 37 and 38 °C. The acquired thermal tolerance (ATT) in intact seedlings was over 50% at 35 °C → 50 °C and 37 °C → 50 °C, but in cell viability test it ranged from 2.75% (Ae. triuncialis) to 32.87 (Bezostaya-1) at 35 °C, and from 2.82% (Ae. triuncialis) to 37.82 (Bezostaya-1) at 37 °C. Ae. triuncialis was most sensitive genotype in both ATT determination. In electrophoretic profiles of proteins, while some HSPs were newly synthesized, some normal cellular proteins disappeared at 37 °C and 37 °C → 50 °C compared to 23 °C. The number of low molecular weight (LMW) HSPs were more than intermediate- (IMW) and high- (HMW) HSPs. The genotypes had both common (12 HSPs between at least two genotypes) and genotype-specific (33 HSPs) LMW HSPs. The common HSP of 19.8 kDa (pI 6.5) was synthesized in Bezostaya-1, Çukurova-86, Diyarbakır-81, Ae. biuncialis and Ae. umbellulata. Bezostaya-1 is the only genotype that synthesized 12 IMW and 2 HMW HSPs at 37 °C → 50 °C. Ae. triuncialis had only two common LMW HSPs [22.1 (pI 7.1) and 24.2 kDa (pI 6.5)].

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Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X

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Editor(s)-in-Chief: Elekes, Károly

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

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Acta Biologica Hungarica
Language English
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Foundation		 1950
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Programme		 changed title
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Founder Magyar Tudományos Akadémia
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ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)

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