Acta Biologica Hungarica
Volume/Issue:
Volume 57: Issue 2
Targeting dsRNA-specific single-chain Fv antibody fragments to different cellular locations in Nicotiana tabacum L.
Authors:
B. Morgun Institute of Plant Biology, Biological Research Centre Szeged, Hungary

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A. Richter Institut für Physikalische Biologie Heinrich-Heine-Universität Düsseldorf, FRG

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D. Deshmukh Institute of Plant Biology, Biological Research Centre Szeged, Hungary

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V. Stepanyuk Institute of Plant Biology, Biological Research Centre Szeged, Hungary

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Katalin Kálai Department of Plant Physiology and Plant Biochemistry Corvinus University of Budapest, Hungary

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G. Nagy Department of Plant Physiology and Plant Biochemistry Corvinus University of Budapest, Hungary

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L. Hufnagel Department of Mathematics and Informatics Corvinus University of Budapest, Hungary

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Noémi Lukács Institute of Plant Biology, Biological Research Centre Institut für Physikalische Biologie Department of Plant Physiology and Plant Biochemistry Szeged, Hungary Heinrich-Heine-Universität Düsseldorf, FRG Corvinus University of Budapest, Hungary

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Pages:
247–259
Online Publication Date:
08 Jun 2006
Publication Date:
01 Jun 2006
Article Category:
Research Article
DOI:
https://doi.org/10.1556/abiol.57.2006.2.11
Restricted access

Expression of antibodies or antibody fragments in plants is a useful tool for producing active antibody derivatives for diagnostic or pharmaceutical purposes as well as for immunomodulation. We investigated the effect of cellular expression site on the stability and yield of double-stranded RNA (dsRNA)-specific single-chain Fv-fragments (scFv) in transgenic tobacco. Two antibodies (J2 and P6) belonging to the V23(J558) heavy chain variable gene family but differing in the light chain variable domain were used. scFvs were targeted to the cytoplasm - with or without anchoring them in the plasma membrane -, into the endoplasmic reticulum (ER) and to the apoplast. Although high mRNA concentrations were detected in all cases, scFv proteins accumulated only when scFvs were made ER-resident by appropriate signal sequences. When the ER retention signal was removed to allow scFv-secretion to the apoplast, no scFv-proteins were detected. Despite the strong homology of the VH-sequences of J2 and P6 antibodies, only P6 provided a stable scFv scaffold for intracytoplasmic expression. J2-scFv could not be stabilised either by adding a C-terminal stabilisation signal or by anchoring the protein on the cytoplasmic side of the plasma membrane (PM). It was found that dsRNA-specific J2-scFvs are active in vivo and enhance PotatoVirus Y induced symptoms in infected tobacco. This is the first report describing the expression and biological effect of RNA-specific antibodies in plants.

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Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X

Senior editors

Editor(s)-in-Chief: Elekes, Károly

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

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Acta Biologica Hungarica
Language English
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Year of
Foundation		 1950
Publication
Programme		 changed title
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Founder Magyar Tudományos Akadémia
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
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ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)

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