Acta Biologica Hungarica
Volume/Issue:
Volume 58: Issue Issue Supplement-1
Application of the dual-tagging gene trap method combined with a novel automatic selection system to identify genes involved in germ cell development in Drosophila melanogaster
Authors:
P. Vilmos Biological Research Center of the Hungarian Academy of Sciences Institute of Genetics Szeged Hungary

Search for other papers by P. Vilmos in
Current site
Google Scholar
PubMed Close
,
L. Henn Biological Research Center of the Hungarian Academy of Sciences Institute of Genetics Szeged Hungary

Search for other papers by L. Henn in
Current site
Google Scholar
PubMed Close
,
M. Szathmári Biological Research Center of the Hungarian Academy of Sciences Institute of Genetics Szeged Hungary

Search for other papers by M. Szathmári in
Current site
Google Scholar
PubMed Close
,
T. Lukácsovich University of California Irvine Department of Developmental and Cell Biology Irvine USA

Search for other papers by T. Lukácsovich in
Current site
Google Scholar
PubMed Close
,
L. Sipos Biological Research Center of the Hungarian Academy of Sciences Institute of Genetics Szeged Hungary

Search for other papers by L. Sipos in
Current site
Google Scholar
PubMed Close
, and
M. Erdélyi Biological Research Center of the Hungarian Academy of Sciences Institute of Genetics Szeged Hungary

Search for other papers by M. Erdélyi in
Current site
Google Scholar
PubMed Close
Pages:
81–94
Online Publication Date:
24 Oct 2007
Publication Date:
01 Nov 2007
Article Category:
Research Article
DOI:
https://doi.org/10.1556/abiol.58.2007.suppl.7
Keywords:
Drosophila melanogaster ; germ cell; mutagenesis; SOT system; pebbled
Restricted access

The passage of highly specialized germ cells to future generations is essential for the maintenance of species. To date, conventional genetic screens identified relatively few genes that are involved in germ cell development. We aimed to identify germ line specific genes on the X chromosome of Drosophila melanogaster by the application of a new method: the dual-tagging gene-trap system (GT). A modified version of the gene-trap element was used in our experiments and the resulting insertional mutants were screened for grandchild-less phenotype with the help of the attached-X system and a sensitized genetic background developed in our laboratory. Among the 800 insertions mapped to the X chromosome 33 new mutations were identified that exhibited grandchild-less phenotype, 6 gave visible phenotype and 12 were conditional lethal. The cloning of a selected group of the 33 lines showing grandchild-less pheno-type confirmed that we have identified new candidates for genes involved in germ cell development. One of them named pebbled ( peb ) is discussed in details in this paper. Finally, we also describe a novel automatic selection system developed in our laboratory which enables the extension of the GT mutagenesis to the autosomes.

  • Ashburner, M. (1989) Drosophila, a Laboratory Handbook . Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

    Ashburner M. , '', in Drosophila, a Laboratory Handbook , (1989 ) -.

  • Bardsley, A., MacDonald, K., Boswell, R. E. (1993) Distribution of tudor protein in the Drosophila embryo suggests separation of function based on site of localization. Development 119 , 207–219.

    Boswell R. E. , 'Distribution of tudor protein in the Drosophila embryo suggests separation of function based on site of localization ' (1993 ) 119 Development : 207 -219 .

    • Search Google Scholar

  • Barker, D. D., Wang, C., Moore, J., Dickinson, L. K., Lehmann, R. (1992) Pumilio is essential for function but not for distribution of the Drosophila abdominal determinant Nanos. Genes Dev. 6 , 2312–2326.

    Lehmann R. , 'Pumilio is essential for function but not for distribution of the Drosophila abdominal determinant Nanos ' (1992 ) 6 Genes Dev. : 2312 -2326 .

    • Search Google Scholar

  • Bingham, P. M., Chapman, C. H. (1986) Evidence that white-blood is a novel type of temperature-sensitive mutation resulting from temperature-dependent effects of a transposon insertion on formation of white transcripts. EMBO J. 5 , 3343–3351.

    Chapman C. H. , 'Evidence that white-blood is a novel type of temperature-sensitive mutation resulting from temperature-dependent effects of a transposon insertion on formation of white transcripts ' (1986 ) 5 EMBO J. : 3343 -3351 .

    • Search Google Scholar

  • Bryan, G., Garza, D., Hartl, D. (1990) Insertion and excision of the transposable element mariner in Drosophila . Genetics 125 , 103–114.

    Hartl D. , 'Insertion and excision of the transposable element mariner in Drosophila ' (1990 ) 125 Genetics : 103 -114 .

    • Search Google Scholar

  • Ding, D., Lipshitz, H. D. (1993) A molecular screen for polar-localised maternal RNAs in the early embryo. Zygote 1 , 257–271.

    Lipshitz H. D. , 'A molecular screen for polar-localised maternal RNAs in the early embryo ' (1993 ) 1 Zygote : 257 -271 .

    • Search Google Scholar

  • Erdelyi, M., Mathe, E., Szabad, J. (1997) Genetic and developmental analysis of mutant Ketel alleles that identify the Drosophila importin-beta homologue. Acta Biol. Hung. 48 , 323–338.

    Szabad J. , 'Genetic and developmental analysis of mutant Ketel alleles that identify the Drosophila importin-beta homologue ' (1997 ) 48 Acta Biol. Hung. : 323 -338 .

    • Search Google Scholar

  • Fischer-Vize, J. A., Rubin, G. M., Lehmann, R. (1992) The fat facets gene is required for Drosophila eye and embryo development. Development 116 , 985–1000.

    Lehmann R. , 'The fat facets gene is required for Drosophila eye and embryo development ' (1992 ) 116 Development : 985 -1000 .

    • Search Google Scholar

  • Frank, L. H., Rushlow, Ch. (1996) A group of genes required for maintenance of the amnioserosa tissue in Drosophila . Development 122 , 1343–1352.

    Rushlow Ch. , 'A group of genes required for maintenance of the amnioserosa tissue in Drosophila ' (1996 ) 122 Development : 1343 -1352 .

    • Search Google Scholar

  • Gans, M., Audit, C., Masson, M. (1975) Isolation and characterization of sex-linked female-sterile mutants in Drosophila melanogaster . Genetics 81 , 683–704.

    Masson M. , 'Isolation and characterization of sex-linked female-sterile mutants in Drosophila melanogaster ' (1975 ) 81 Genetics : 683 -704 .

    • Search Google Scholar

  • Hacker, U., Nystedt, S., Barmchi, M. P., Horn, C., Wimmer, E. A. (2003) piggyBac -based insertional mutagenesis in the presence of stably integrated P elements in Drosophila . PNAS 100 , 7720–7725.

    Wimmer E. A. , 'piggyBac-based insertional mutagenesis in the presence of stably integrated P elements in Drosophila ' (2003 ) 100 PNAS : 7720 -7725 .

    • Search Google Scholar

  • Howard, K., Jaglarz, M., Zhang, N., Shah, J., Warrior, R. (1993) Migration of Drosophila germ cells: analysis using enhancer trap lines. Development Supplement , 213–218.

  • Jankovics, F., Sinka, R., Lukacsovich, T., Erdelyi, M. (2002) MOESIN crosslinks actin and cell membrane in Drosophila oocytes and is required for OSKAR anchoring. Curr. Biol. 10 , 2060–2065.

    Erdelyi M. , 'MOESIN crosslinks actin and cell membrane in Drosophila oocytes and is required for OSKAR anchoring ' (2002 ) 10 Curr. Biol. : 2060 -2065 .

    • Search Google Scholar

  • Jaglarz, M. K., Howard, K. R. (1994) Primordial germ cell migration in Drosophila melanogaster is controlled by somatic tissue. Development 120 , 83–89.

    Howard K. R. , 'Primordial germ cell migration in Drosophila melanogaster is controlled by somatic tissue ' (1994 ) 120 Development : 83 -89 .

    • Search Google Scholar

  • Jongens, T. A., Hay, B., Jan, L. Y., Jan, Y. N. (1992) The germ cell-less gene product: a posteriorly localized component necessary for germ cell development in Drosophila. Cell 70 , 569–584.

    Jan Y. N. , 'The germ cell-less gene product: a posteriorly localized component necessary for germ cell development in Drosophila ' (1992 ) 70 Cell : 569 -584 .

    • Search Google Scholar

  • Kobayashi, S., Amikura, R., Okada, M. (1993) Presence of mitochondrial large ribosomal RNA outside mitochondria in germ plasm of Drosophila melanogaster . Science 260 , 1521–1524.

    Okada M. , 'Presence of mitochondrial large ribosomal RNA outside mitochondria in germ plasm of Drosophila melanogaster ' (1993 ) 260 Science : 1521 -1524 .

    • Search Google Scholar

  • Liao, G., Rehm, E. J., Rubin, G. M. (2000) Insertion site preferences of the P transposable element in Drosophila melanogaster . PNAS 97 , 3347–3351.

    Rubin G. M. , 'Insertion site preferences of the P transposable element in Drosophila melanogaster ' (2000 ) 97 PNAS : 3347 -3351 .

    • Search Google Scholar

  • Lukacsovich, T., Asztalos, Z., Yamamoto, D. (2000) Functional genomics in Drosophila melanogaster by gene-trapping. Current Genomics 1 , 323–338.

    Yamamoto D. , 'Functional genomics in Drosophila melanogaster by gene-trapping ' (2000 ) 1 Current Genomics : 323 -338 .

    • Search Google Scholar

  • Lukacsovich, T., Asztalos, Z., Awano, W., Baba, K., Kondo, S., Niwa, S., Yamamoto, D. (2001) Dual-tagging gene-trap of novel genes in Drosophila melanogaster . Genetics , 157 , 727–742.

    Yamamoto D. , 'Dual-tagging gene-trap of novel genes in Drosophila melanogaster ' (2001 ) 157 Genetics : 727 -742 .

    • Search Google Scholar

  • Moore, L. A., Broihier, H. T., Van Doren, M., Lunsford, L. B., Lehmann, R. (1998) Identification of genes controlling germ cell migration and embryonic gonad formation in Drosophila . Development 125 , 667–678.

    Lehmann R. , 'Identification of genes controlling germ cell migration and embryonic gonad formation in Drosophila ' (1998 ) 125 Development : 667 -678 .

    • Search Google Scholar

  • Nakamura, A., Sato, K., Hanyu-Nakamura, K. (1996) Drosophila cup is an eIF4E binding protein that associates with bruno and regulates oskar mRNA translation in oogenesis. Dev. Cell 6 , 69–78.

    Hanyu-Nakamura K. , 'Drosophila cup is an eIF4E binding protein that associates with bruno and regulates oskar mRNA translation in oogenesis ' (1996 ) 6 Dev. Cell : 69 -78 .

    • Search Google Scholar

  • Raff, J. W., Whitfield, W. G. F., Glover, D. M. (1990) Two distinct mechanisms localise cyclin B transcripts in syncytial Drosophila embryos. Development 110 , 1–13.

    Glover D. M. , 'Two distinct mechanisms localise cyclin B transcripts in syncytial Drosophila embryos ' (1990 ) 110 Development : 1 -13 .

    • Search Google Scholar

  • Reed, B. H., Wilk, R., Lipshitz, H. D. (2001) Downregulation of Jun kinase signaling in the amnioserosa is essential for dorsal closure of the Drosophila embryo. Curr. Biol. 11 , 1098–1108.

    Lipshitz H. D. , 'Downregulation of Jun kinase signaling in the amnioserosa is essential for dorsal closure of the Drosophila embryo ' (2001 ) 11 Curr. Biol. : 1098 -1108 .

    • Search Google Scholar

  • Rørth, P. (1998) Gal4 in the Drosophila female germline. Merc. Dev. 78 , 113–118.

    Rørth P. , 'Gal4 in the Drosophila female germline ' (1998 ) 78 Merc. Dev. : 113 -118 .

    • Search Google Scholar

  • Samson, M. L., Lisbin, M. J., White, K. (1995) Two distinct temperature-sensitive alleles at the elav locus of Drosophila are suppressed nonsense mutations of the same tryptophan codon. Genetics 141 , 1101–1111.

    White K. , 'Two distinct temperature-sensitive alleles at the elav locus of Drosophila are suppressed nonsense mutations of the same tryptophan codon ' (1995 ) 141 Genetics : 1101 -1111 .

    • Search Google Scholar

  • Santos, A. C., Lehmann, R. (2004) Germ cell specification and migration in Drosophila and beyond. Curr. Biol. 14 , R578–R589.

    Lehmann R. , 'Germ cell specification and migration in Drosophila and beyond ' (2004 ) 14 Curr. Biol. : R578 -R589 .

    • Search Google Scholar

  • Saville, K. J., Warren, W. D., Atkinson, P. W., O’Brochta, D. A. (1999) Integration specificity of the hobo element of Drosophila melanogaster is dependent on sequences flanking the integration site. Genetica 105 , 133–147.

    O’Brochta D. A. , 'Integration specificity of the hobo element of Drosophila melanogaster is dependent on sequences flanking the integration site ' (1999 ) 105 Genetica : 133 -147 .

    • Search Google Scholar

  • Snee, M. J., MacDonald, P. M. (2004) Live imaging of nuage and polar granules: evidence against a precursor-product relationship and a novel role for Oskar in stabilization of polar granule components. J. Cell Sci. 117 , 2109–2120.

    MacDonald P. M. , 'Live imaging of nuage and polar granules: evidence against a precursor-product relationship and a novel role for Oskar in stabilization of polar granule components ' (2004 ) 117 J. Cell Sci. : 2109 -2120 .

    • Search Google Scholar

  • Stark, C., Breitkreutz, B. J., Reguly, T., Boucher, L., Breitkreutz, A., Tyers, M. (2006) BioGRID: A general repository for interaction datasets. Nucleic Acids Res. 1 , D535–D539.

    Tyers M. , 'BioGRID: A general repository for interaction datasets ' (2006 ) 1 Nucleic Acids Res. : D535 -D539 .

    • Search Google Scholar

  • Starz-Gaiano, M., Cho, N. K., Forbes, A., Lehmann, R. (2001) Spatially restricted activity of a Drosophila lipid phosphatase guides migrating germ cells. Development 128 , 983–991.

    Lehmann R. , 'Spatially restricted activity of a Drosophila lipid phosphatase guides migrating germ cells ' (2001 ) 128 Development : 983 -991 .

    • Search Google Scholar

  • St. Johnston, D., Beuchle, D., Nusslein-Volhard, C. (1991) Staufen, a gene required to localize maternal RNAs in the Drosophila egg. Cell 66 , 51–63.

    Nusslein-Volhard C. , 'Staufen, a gene required to localize maternal RNAs in the Drosophila egg ' (1991 ) 66 Cell : 51 -63 .

    • Search Google Scholar

  • Sullivan, W., Ashburner, M., Hawley, R. S. (2000) Drosophila Protocols . Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA.

    Hawley R. S. , '', in Drosophila Protocols , (2000 ) -.

  • Szuperák, M., Zvara, Á., Erdélyi, M. (2005) Identification of germ plasm-enriched mRNAs in Drosophila melanogaster by the cDNA microarray technique. Gene Expression Patterns 5 , 717–723.

    Erdélyi M. , 'Identification of germ plasm-enriched mRNAs in Drosophila melanogaster by the cDNA microarray technique ' (2005 ) 5 Gene Expression Patterns : 717 -723 .

    • Search Google Scholar

  • Teixeira, L., Rabouille, C., Rørth, P., Ephrussi, A., Vanzo, N. F. (2003) Drosophila Perilipin/ADRP homologue Lsd2 regulates lipid metabolism Mech. Dev. 120 , 1071–1081.

    Vanzo N. F. , 'Drosophila Perilipin/ADRP homologue Lsd2 regulates lipid metabolism ' (2003 ) 120 Mech. Dev. : 1071 -1081 .

    • Search Google Scholar

  • Tomancak, P., Beaton, A., Weiszmann, R., Kwan, E., Shu, S., Lewis, S. E., Richards, S., Ashburner, M., Hartenstein, V., Celniker, S. E., Rubin, G. M. (2002) Systematic determination of patterns of gene expression during Drosophila embryogenesis. Genome Biol. 2002;3(12):RESEARCH0088.

  • Wang, C., Lehmann, R. (1991) Nanos is the localized posterior determinant in Drosophila. Cell 66 , 637–647.

    Lehmann R. , 'Nanos is the localized posterior determinant in Drosophila ' (1991 ) 66 Cell : 637 -647 .

    • Search Google Scholar

  • Warrior, R. (1994) Primordial germ cell migration and the assembly of the Drosophila embryonic gonad. Dev. Biol. 166 , 180–194.

    Warrior R. , 'Primordial germ cell migration and the assembly of the Drosophila embryonic gonad ' (1994 ) 166 Dev. Biol. : 180 -194 .

    • Search Google Scholar

  • Wilk, R., Pickup, A. T., Hamilton, J. K., Reed, B. H., Lipshitz, H. D. (2004) Dose-sensitive autosomal modifiers identify candidate genes for tissue autonomous and tissue nonautonomous regulation by the Drosophila nuclear zinc-finger protein, Hindsight. Genetics 168 , 281–300.

    Lipshitz H. D. , 'Dose-sensitive autosomal modifiers identify candidate genes for tissue autonomous and tissue nonautonomous regulation by the Drosophila nuclear zinc-finger protein, Hindsight ' (2004 ) 168 Genetics : 281 -300 .

    • Search Google Scholar

  • Yohn, C. B., Pusateri, L., Barbosa, V., Lehmann, R. (2003) l(3)malignant brain tumor and three novel genes are required for Drosophila germ-cell formation. Genetics 165 , 1889–1900.

    Lehmann R. , 'l(3)malignant brain tumor and three novel genes are required for Drosophila germ-cell formation ' (2003 ) 165 Genetics : 1889 -1900 .

    • Search Google Scholar

  • Yip, R. M. L., Lamka, M. L., Lipshitz, H. D. (1997) Control of germ-band retraction in Drosophila by the zinc-finger protein HINDSIGHT. Development 124 , 2129–2141.

    Lipshitz H. D. , 'Control of germ-band retraction in Drosophila by the zinc-finger protein HINDSIGHT ' (1997 ) 124 Development : 2129 -2141 .

    • Search Google Scholar

  • Zhang, N., Zhang, J., Cheng, Y., Howard, K. (1996) Identification and genetic analysis of wunen , a gene guiding Drosophila melanogaster germ cell migration. Genetics 143 , 1231–1241.

    Howard K. , 'Identification and genetic analysis of wunen, a gene guiding Drosophila melanogaster germ cell migration ' (1996 ) 143 Genetics : 1231 -1241 .

    • Search Google Scholar
  • Collapse
  • Expand
Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X

Senior editors

Editor(s)-in-Chief: Elekes, Károly

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

Acta Biologica Hungarica
P.O. Box 35
H-8237 Tihany, Hungary
Phone: (36 87) 448 244 ext. 103
Fax: (36 87) 448 006
E-mail: elekes@tres.blki.hu

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture
  • Biology and Environmental Sciences
  • Elsevier BIOBASE
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Acta Biologica Hungarica
Language English
Size  
Year of
Foundation		 1950
Publication
Programme		 changed title
Volumes
per Year		  
Issues
per Year		  
Founder Magyar Tudományos Akadémia
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Nov 2024 38 0 0
Dec 2024 18 0 0
Jan 2025 40 0 0
Feb 2025 33 0 0
Mar 2025 30 0 0
Apr 2025 13 0 0
May 2025 2 0 0