The effect of copper excess on growth, H 2 O 2 level and peroxidase activities were studied in maize shoots. Ten-day-old seedlings were cultured in nutrient solution that contained Cu 2+ ions at various concentrations (50 and 100 μM) for seven days. High concentrations of Cu 2+ ions caused significant decrease both in matter production and elongation of maize shoots. In addition, treatment with CuSO 4 increased levels of H 2 O 2 and induced changes in several peroxidase activities. Moreover, the disturbance of the physiological parameters was accompanied by the modulation of the peroxidase activities: GPX (Guaiacol peroxidase, EC 1.11.1.7), CAPX (Coniferyl alcohol peroxidase, EC 1.11.1.4) and APX (Ascorbate peroxidase, EC.1.11.1.11). Furthermore, this modulation becomes highly significant, especially, in the presence of 100 μM of CuSO 4 .
Alloway, B. J. (1995) Heavy Metals in Soils . Second Edition, Blackie Academic and Professional, London.
Alloway B. J. , '', in Heavy Metals in Soils , (1995 ) -.
Alvarez, M. E., Penel, R. I., Meijer, P. J., Ishikaw, A., Dixon, R. A., Lamb, C. (1998) Reactive oxygen intermediate a systemic signal network in the establishment of plant immunity. Cell 92 , 773–784.
Lamb C. , 'Reactive oxygen intermediate a systemic signal network in the establishment of plant immunity ' (1998 ) 92 Cell : 773 -784 .
Baccouche, S., Chaoui, A., El Ferjani, E. (1998) Nickel induced oxidative damage and antioxidant responses in Zea mays shoots. Plant Physiol. Biochem. 36 , 6896–6894.
Ferjani E. , 'Nickel induced oxidative damage and antioxidant responses in Zea mays shoots ' (1998 ) 36 Plant Physiol. Biochem. : 6896 -6894 .
Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principale of protein-dye binding. Anal. Biochem. 72 , 248–258.
Bradford M. M. , 'A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principale of protein-dye binding ' (1976 ) 72 Anal. Biochem. : 248 -258 .
Chen, E. L., Chen, Y. A., Chen, L. M., Liu, Z. H. (2002) Effect of copper on peroxidase activity and lignin content in Raphanus sativus . Plant Physiol. Biochem. 40 , 439–444.
Liu Z. H. , 'Effect of copper on peroxidase activity and lignin content in Raphanus sativus ' (2002 ) 40 Plant Physiol. Biochem. : 439 -444 .
Cuypers, A., Vangronsveld, J., Clijsters, H. (2000) Biphasic effect of copper on the ascorbate-glutathione pathway in primary leaves of Phaseolus vulgaris seedlings during early stages of metal assimilation. Physiol. Plant. 110 , 512–517.
Clijsters H. , 'Biphasic effect of copper on the ascorbate-glutathione pathway in primary leaves of Phaseolus vulgaris seedlings during early stages of metal assimilation ' (2000 ) 110 Physiol. Plant. : 512 -517 .
Elstner, E. F., Wagner, G. A., Schütz, W. (1988) Activated oxygen in green plants in relation to stress situations. Curr. Topics Plant Biochem. 36 , 873–877.
Schütz W. , 'Activated oxygen in green plants in relation to stress situations ' (1988 ) 36 Curr. Topics Plant Biochem. : 873 -877 .
Fielding, J. L., Hall, J. L. (1978) A biochemical and cytochemical study of peroxidase activity in roots of Pisum sativum . J. Exp. Bot. 29 , 979–986.
Hall J. L. , 'A biochemical and cytochemical study of peroxidase activity in roots of Pisum sativum ' (1978 ) 29 J. Exp. Bot. : 979 -986 .
Gabbrielli, R. M., Pandolfini, T. M., Vergano, O., Palandri, M. R. (1990) Comparison of two serpentine species with different nickel tolerance strategies. Plant Soil 122 , 271–277.
Palandri M. R. , 'Comparison of two serpentine species with different nickel tolerance strategies ' (1990 ) 122 Plant Soil : 271 -277 .
Gupta, M., Cuypers, A., Vangronsveld, J., Clijsters, H. (1999) Copper affects the enzymes of the ascorbate-glutathione cycle and its related metabolites in roots of Phaseolus vulgaris . Physiol Plant. 106 , 262–267.
Clijsters H. , 'Copper affects the enzymes of the ascorbate-glutathione cycle and its related metabolites in roots of Phaseolus vulgaris ' (1999 ) 106 Physiol Plant. : 262 -267 .
Halliwell, B., Gutteridge, J. M. C. (1984) Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem. J. 219 , 1–14.
Gutteridge J. M. C. , 'Oxygen toxicity, oxygen radicals, transition metals and disease ' (1984 ) 219 Biochem. J. : 1 -14 .
Jouili, H., El Ferjani, E. (2003) Changes in antioxidant and lignifying enzyme activities in sunflower roots ( Helianthus annuus L.) stressed with copper excess. C. R. Biologies 326 , 639–644.
Ferjani E. , 'Changes in antioxidant and lignifying enzyme activities in sunflower roots (Helianthus annuus L.) stressed with copper excess ' (2003 ) 326 C. R. Biologies : 639 -644 .
Jouili, H., El Ferjani, E. (2004) Effect of copper excess on superoxide dismutase, catalase, and peroxidase activities in sunflower seedlings ( Helianthus annuus L.). Acta physiologiae Plantarum 26 , 29–35.
Ferjani E. , 'Effect of copper excess on superoxide dismutase, catalase, and peroxidase activities in sunflower seedlings (Helianthus annuus L.) ' (2004 ) 26 Acta physiologiae Plantarum : 29 -35 .
Lin, C. C., Chen, L. M., Liu, Z. H. (2005) Rapid effect of copper on lignin biosynthesis in soybean roots. Plant Sci. 168 , 858–861.
Liu Z. H. , 'Rapid effect of copper on lignin biosynthesis in soybean roots ' (2005 ) 168 Plant Sci. : 858 -861 .
Mazhoudi, S., Chaoui, A., Gorbal, M. H., El Ferjani, E. (1997) Response of antioxidant enzymes to excess copper in tomato ( Lycopersion esculentum , Mill.). Plant Sci. 127 , 182–186.
Ferjani E. , 'Response of antioxidant enzymes to excess copper in tomato (Lycopersion esculentum, Mill.) ' (1997 ) 127 Plant Sci. : 182 -186 .
Mocquot, B., Vangronseveld, J., Clijsters, H., Mench, M. (1996) Copper toxicity in young maize ( Zea mays L.) plants: effects on growth, mineral and chlorophyll contents and enzyme activities. Plant and Soil 182 , 287–300.
Mench M. , 'Copper toxicity in young maize (Zea mays L.) plants: effects on growth, mineral and chlorophyll contents and enzyme activities ' (1996 ) 182 Plant and Soil : 287 -300 .
Nakano, Y., Asada, K. (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplast. Plant Cell Physiol. 22 , 423–430.
Asada K. , 'Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplast ' (1981 ) 22 Plant Cell Physiol. : 423 -430 .
Pandolfini, T., Gabbrielli, R., Comparini, C. (1992) Nickel toxicity and peroxidase activity in seedlings of Triticum aestivum L. Plant Cell Environ. 15 , 719–725.
Comparini C. , 'Nickel toxicity and peroxidase activity in seedlings of Triticum aestivum L. ' (1992 ) 15 Plant Cell Environ. : 719 -725 .
Pedereńo, M. A., Barcelo, A. R., Sabatter, F., Munoz, R. (1989) Control by pH of cell wall peroxidase activity involved in lignification. Plant Cell Physiol. 30 , 237–241.
Munoz R. , 'Control by pH of cell wall peroxidase activity involved in lignification ' (1989 ) 30 Plant Cell Physiol. : 237 -241 .
Quiroga, M., Guerrero, C., Botella, M. A., Barceló, Amaya, I., Medina, M. I., Alonso, F. J., Forchetti, S. M., Tigier, H., Valpuesta, V. (2000) A tomato peroxidase involved in the synthesis of lignin and suberin. Plant Physiol. 122 , 1119–1127.
Valpuesta V. , 'A tomato peroxidase involved in the synthesis of lignin and suberin ' (2000 ) 122 Plant Physiol. : 1119 -1127 .
Sandmann, G., Böger, P. (1980) Copper-mediated lipid peroxidation processes in photosynthetic membranes. Plant Physiol. 66 , 797–800.
Böger P. , 'Copper-mediated lipid peroxidation processes in photosynthetic membranes ' (1980 ) 66 Plant Physiol. : 797 -800 .
Sanita Di Troppi, L., Gabbrielli, R. (1999) Response to cadmium in higher plants. Environ. Exp. Bot. 41 , 105–130.
Gabbrielli R. , 'Response to cadmium in higher plants ' (1999 ) 41 Environ. Exp. Bot. : 105 -130 .
Scarponil, L., Perucci, P. (1984) Effects of some metals and related metal organic compounds on ALA-deshydratase activity of corn. Plant and Soil 79 , 69–75.
Perucci P. , 'Effects of some metals and related metal organic compounds on ALA-deshydratase activity of corn ' (1984 ) 79 Plant and Soil : 69 -75 .
Schützendübel, A., Nikolova, I. P., Rudolf, C., Polle, A. (2002) Cadmium and H 2 O 2 -induced oxidative stress in Populus × canescens roots. Plant Physiol. Biochem. 40 , 577–584.
Polle A. , 'Cadmium and H2O2-induced oxidative stress in Populus×canescens roots ' (2002 ) 40 Plant Physiol. Biochem. : 577 -584 .
Sergiev, I., Alexieva, V., Karanov, E. (1997) Effect of spermine, atrazine and combination between them on some endogenous protective systems and stress markers in plants. Comt. Rend. Acad. Bulg. Sci. 51 , 121–124.
Karanov E. , 'Effect of spermine, atrazine and combination between them on some endogenous protective systems and stress markers in plants ' (1997 ) 51 Comt. Rend. Acad. Bulg. Sci. : 121 -124 .
Sgherri, C., Milone, M. T. A., Clijsters, H., Navari-Izzo, F. (2001) Antioxidative enzymes in two wheat cultivars, differently sensitive to drought and subjected to subsymptomatic copper doses. J. Plant Physiol. 158 , 1439–1447.
Navari-Izzo F. , 'Antioxidative enzymes in two wheat cultivars, differently sensitive to drought and subjected to subsymptomatic copper doses ' (2001 ) 158 J. Plant Physiol. : 1439 -1447 .
Shigeoka, S., Ishikawa, T., Tamoi, M., Miyagawa, Y., Takeda, T., Yabuta, Y. R., Kazuya, Y. (2002) Regulation and function of ascorbate peroxidase isoenzymes. J. Exp. Bot. 53 , 1305–1319.
Kazuya Y. , 'Regulation and function of ascorbate peroxidase isoenzymes ' (2002 ) 53 J. Exp. Bot. : 1305 -1319 .
Van Assche, F., Clijsters, H. (1990) Effects of metals on enzyme activity in plants. Plant Cell Environ. 13 , 195–206.
Clijsters H. , 'Effects of metals on enzyme activity in plants ' (1990 ) 13 Plant Cell Environ. : 195 -206 .
Velikova, V., Yordanov, I., Edreva, Q. (2000) Oxidative and some antioxidant systems in acid raintreated bean plants. Protection role of exogenous polyamines. Plant Science 151 , 59–66.
Edreva Q. , 'Oxidative and some antioxidant systems in acid raintreated bean plants. Protection role of exogenous polyamines ' (2000 ) 151 Plant Science : 59 -66 .
Wainwright, S. J., Woolhouse, H. W. (1977) Some physiological aspects of copper and zinc tolerance in Agrostis tenius sibth: cell elongation and membrane damage. J. Exp. Bot. 281 , 1029–1036.
Woolhouse H. W. , 'Some physiological aspects of copper and zinc tolerance in Agrostis tenius sibth: cell elongation and membrane damage ' (1977 ) 281 J. Exp. Bot. : 1029 -1036 .
Yruela, I. (2005) Copper in plants. Braz. J. Plant Physiol. 171 , 145–156.
Yruela I. , 'Copper in plants ' (2005 ) 171 Braz. J. Plant Physiol. : 145 -156 .