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  • 1 Eötvös Loránd University Department of Chemical Technology and Environmental Chemistry Budapest Hungary
  • 2 Hungarian Academy of Science Plant Protection Institute Budapest Hungary
  • 3 Research Institute for Animal Breeding and Nutrition Herceghalom Hungary
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The effect of Se(IV) and Cu(II) ions on the antibacterial activity of aflatoxins and ochratoxin A (mycotoxins) was studied in BioArena as a complex bioautographic system. In the presence of 0.23 and 0.46 mg/100 mL Se(IV) the inhibition zones of mycotoxins were decreased, however, lower concentration (0.046 mg/100 mL) increased the antibacterial effect of aflatoxin B1. Cu(II) (1.53 mg/100 mL) enhanced the toxicity of mycotoxins. The results supported the possible role of formaldehyde and its reaction products (e.g. 1 O 2 , O 3 ) in the antibacterial-toxic action of mycotoxins. Cu(II) can probably generate and mobilise the formaldehyde molecules and so it could increase the toxicity with its potential reaction products. It is possible that the enzymatic or spontaneous methylation of Se(IV) takes place through formaldehyde, which may cause partial formaldehyde depletion in the system. The enhanced antibacterial effect at low concentration Se(IV) is overlapping with the often experienced prooxidant effect in cases of natural antioxidants.

  • Billes, F., Móricz, Á. M., Tyihák, E., Mikosch, H. (2005) Simulated vibrational spectra of aflatoxins and their demethylated products and the estimation of the energies of the demethylation reactions. Spectrochim. Acta A Mol. Biomol. Spectrosc. 64 , 600–622.

    Mikosch H. , 'Simulated vibrational spectra of aflatoxins and their demethylated products and the estimation of the energies of the demethylation reactions ' (2005 ) 64 Spectrochim. Acta A Mol. Biomol. Spectrosc. : 600 -622.

    • Search Google Scholar
  • Harless, W., Crowell, E., Abraham, J. (2006) Anemia and neutropenia associated with copper deficiency of unclear etiology. Am. J. Hematol. 81 , 546–549.

    Abraham J. , 'Anemia and neutropenia associated with copper deficiency of unclear etiology ' (2006 ) 81 Am. J. Hematol. : 546 -549.

    • Search Google Scholar
  • Huszti, Zs., Tyihák, E. (1986) Formation of formaldehyde from S-adenosyl-L-[methyl-3H]methionine during enzymic transmethylation of histamine. FEBS Lett. 209 , 362–366.

    Tyihák E. , 'Formation of formaldehyde from S-adenosyl-L-[methyl-3H]methionine during enzymic transmethylation of histamine ' (1986 ) 209 FEBS Lett. : 362 -366.

    • Search Google Scholar
  • Lapidot, T., Walker, M. D., Kanner, J. (2002) Antioxidant and prooxidant effects of phenolics on pancreatic β-cells in vitro. J. Agric. Food Chem. 50 , 7220–7225.

    Kanner J. , 'Antioxidant and prooxidant effects of phenolics on pancreatic β-cells in vitro ' (2002 ) 50 J. Agric. Food Chem. : 7220 -7225.

    • Search Google Scholar
  • Mincsovics, E., Garami, M., Kecskés, L., Tapa, B., Végh, Z., Kátay, Gy., Tyihák, E. (1999) Personal overpressured-layer chromatography (OPLC) basic system 50, flexible tool in analytical and semipreparative work. J. AOAC Int. 82 , 587–598.

    Tyihák E. , 'Personal overpressured-layer chromatography (OPLC) basic system 50, flexible tool in analytical and semipreparative work ' (1999 ) 82 J. AOAC Int. : 587 -598.

    • Search Google Scholar
  • Móricz, Á., Otta, K. H., Ott, P. G., Tyihák, E. (2003) Separation and detection of aflatoxins using overpressured-layer chromatography and bioautography. J. Planar Chromatogr. 16 , 417–420.

    Tyihák E. , 'Separation and detection of aflatoxins using overpressured-layer chromatography and bioautography ' (2003 ) 16 J. Planar Chromatogr. : 417 -420.

    • Search Google Scholar
  • Móricz, Á., Otta, K. H., Ott, P. G., Tyihák, E. (2004) New horizon in the characterization of the action of mycotoxins separated by TLC or OPLC. In: Sz. Nyiredy (ed.) Proc. Intern. Symp. on Planar Chromatography , Visegrád, Hungary, pp. 473–485.

  • Neal, G. E. (1995) Genetic implications in the metabolism and toxicity of mycotoxins. Toxicol. Lett. 82–83 , 861–867.

    Neal G. E. , 'Genetic implications in the metabolism and toxicity of mycotoxins ' (1995 ) 82–83 Toxicol. Lett. : 861 -867.

    • Search Google Scholar
  • Olas, B., Wachowich, B., Buczynski, A. (2000) Vitamin C suppresses the cisplatin toxicity on blood platelets. Anticancer Drugs , 11 , 487–493.

    Buczynski A. , 'Vitamin C suppresses the cisplatin toxicity on blood platelets ' (2000 ) 11 Anticancer Drugs : 487 -493.

    • Search Google Scholar
  • Ranjard, L., Prigent-Combaret, C., Favre-Bonte, S., Monnez, C., Nazaret, S., Cournoyer, B. (2004) Characterization of a novel selenium methyltransferase from freshwater bacteria showing strong similarities with the calicheamicin methyltransferase. Biochim. Biophys. Acta. 1679 , 80–85.

    Cournoyer B. , 'Characterization of a novel selenium methyltransferase from freshwater bacteria showing strong similarities with the calicheamicin methyltransferase ' (2004 ) 1679 Biochim. Biophys. Acta. : 80 -85.

    • Search Google Scholar
  • Kaplan, N. M., Palmer, B. F., Revankar, S. G. (2003) Clinical implications of mycotoxins and stachybotrys. Am. J. Med. Sci. 325 , 262–274.

    Revankar S. G. , 'Clinical implications of mycotoxins and stachybotrys ' (2003 ) 325 Am. J. Med. Sci. : 262 -274.

    • Search Google Scholar
  • Richard, J. L., Payne, G. A. (2003) Mycotoxins in plant, animal, and human systems. Task Force Report No. 139 . Council for Agricultural Science and Technology.

  • Rotruck, J. T., Pope, A. L., Ganther, H. E., Swanson, A. B., Hafeman, D. G., Hoekstra, W. G. (1973) Selenium: biochemical role as a component of glutathione peroxidase. Science 73 , 588–590.

    Hoekstra W. G. , 'Selenium: biochemical role as a component of glutathione peroxidase ' (1973 ) 73 Science : 588 -590.

    • Search Google Scholar
  • Stark, A. A. (1980) Mutagenicity and carcinogenicity of mycotoxins: DNA binding as a possible mode of action. Annu. Rev. Microbiol. 34 , 235–262.

    Stark A. A. , 'Mutagenicity and carcinogenicity of mycotoxins: DNA binding as a possible mode of action ' (1980 ) 34 Annu. Rev. Microbiol. : 235 -262.

    • Search Google Scholar
  • Theophanides, T., Anastassopoulou, J. (2002) Copper and carcinogenesis. Crit. Rev. Oncol. Hematol. 42 , 57–64.

    Anastassopoulou J. , 'Copper and carcinogenesis ' (2002 ) 42 Crit. Rev. Oncol. Hematol. : 57 -64.

    • Search Google Scholar
  • Trézl, L., Pipek, J. (1988) Formation of excited formaldehyde in model reactions simulating real biological systems. J. Mol. Struc.-Theochem. 170 , 213–223.

    Pipek J. , 'Formation of excited formaldehyde in model reactions simulating real biological systems ' (1988 ) 170 J. Mol. Struc.-Theochem. : 213 -223.

    • Search Google Scholar
  • Tyihák, E., Bocsi, J., Timár, F., Rácz, G., Szende, B. (2001) Formaldehyde promotes and inhibits the proliferation of cultured tumour and endothelial cells. Cell Proliferation 34 , 135–141.

    Szende B. , 'Formaldehyde promotes and inhibits the proliferation of cultured tumour and endothelial cells ' (2001 ) 34 Cell Proliferation : 135 -141.

    • Search Google Scholar
  • Tyihák, E., Botz, L., Ott, P., Nagy, S., Kocsis, B., Király-Véghely, Zs., Mincsovics, E. (2003) The combination of the overpressured layer chromatography and bioautography and its applications to the analysis of molecules influencing cell proliferation. Chem. Anal. Warsaw 48 , 543–553.

    Mincsovics E. , 'The combination of the overpressured layer chromatography and bioautography and its applications to the analysis of molecules influencing cell proliferation ' (2003 ) 48 Chem. Anal. Warsaw : 543 -553.

    • Search Google Scholar
  • Tyihák, E., Móricz, Á., Ott, P. G. (2004) Role of formaldehyde in antibiotic effect: new approach to mechanism of antibiotic action of some metal ions. In: Metal Ions in Biology and Medicine (Eds Cser, M. A., Sziklai, L. I., Étienne, J. C., Maynard, Y., Centeno, J., Khassanova, I., Collery, P.) John Libbey Eurotext, Paris, Vol. 8, pp. 131–134.

    Ott P. G. , '', in Metal Ions in Biology and Medicine , (2004 ) -.

  • Tyihák, E., Rozsnyay, Zs., Sárdi, É., Gullner, G., Trézl, L., Gáborjányi, R. (1994) Possibility of formation of excited formaldehyde and singlet oxygen in biotic and abiotic stress situations. Acta Biol. Acad. Sci. Hung. 45 , 3–10.

    Gáborjányi R. , 'Possibility of formation of excited formaldehyde and singlet oxygen in biotic and abiotic stress situations ' (1994 ) 45 Acta Biol. Acad. Sci. Hung. : 3 -10.

    • Search Google Scholar
  • Tyihák, E., Trézl, L., Rusznák, I. (1980) Spontaneous N-epsilon-methylation of L-lysine by formaldehyde. Pharmazie 35 , 18–20.

    Rusznák I. , 'Spontaneous N-epsilon-methylation of L-lysine by formaldehyde ' (1980 ) 35 Pharmazie : 18 -20.

    • Search Google Scholar
  • Tyihák, E., Trézl, L., Szende, B. (1998) Formaldehyde cycle and the phases of stress syndrome. Ann. NY Acad. Sci. 851 , 259–270.

    Szende B. , 'Formaldehyde cycle and the phases of stress syndrome ' (1998 ) 851 Ann. NY Acad. Sci. : 259 -270.

    • Search Google Scholar
  • Vinceti, M., Wei, E. T., Malagoli, C., Bergomi, M., Vivoli, G. (2001) Adverse health effects of selenium in humans. Rev. Environ. Health 16 , 233–251.

    Vivoli G. , 'Adverse health effects of selenium in humans ' (2001 ) 16 Rev. Environ. Health : 233 -251.

    • Search Google Scholar
  • Wentworth, P., McDunn, J. E., Wentworth, A. D., Takeuchi, C., Nieva, J., Jones, T., Bautista, C., Ruedi, J. M., Gutierrez, A., Janda, K. D., Babior, B. M., Eschenmoser, A., Lerner, R. A. (2002) Evidence for antibody-catalyzed ozone formation in bacterial killing and inflammation. Science 298 , 2195–2199.

    Lerner R. A. , 'Evidence for antibody-catalyzed ozone formation in bacterial killing and inflammation ' (2002 ) 298 Science : 2195 -2199.

    • Search Google Scholar
  • Whanger, P. D. (2002) Selenocompounds in plants and animals and their biological significance. J. Am. Coll. Nutr. 3 , 223–232.

    Whanger P. D. , 'Selenocompounds in plants and animals and their biological significance ' (2002 ) 3 J. Am. Coll. Nutr. : 223 -232.

    • Search Google Scholar