Acta Biologica Hungarica
Volume/Issue:
Volume 58: Issue 4
Vinpocetine ameliorates acute hepatic damage caused by administration of carbon tetrachloride in rats
Authors:
O. Abdel Salam National Research Centre Department of Pharmacology Tahrir St. Dokki, Cairo Egypt

Search for other papers by O. Abdel Salam in
Current site
Google Scholar
PubMed Close
,
Fatma Hassan Oraby National Research Centre Department of Medical Biochemistry Tahrir St. Dokki, Cairo Egypt

Search for other papers by Fatma Hassan Oraby in
Current site
Google Scholar
PubMed Close
, and
Nabila Hassan National Research Centre Department of Pathology Tahrir St. Dokki, Cairo Egypt

Search for other papers by Nabila Hassan in
Current site
Google Scholar
PubMed Close
Pages:
411–419
Online Publication Date:
06 Nov 2007
Publication Date:
01 Dec 2007
Article Category:
Research Article
DOI:
https://doi.org/10.1556/abiol.58.2007.4.8
Keywords:
Vinpocetine; silymarin; carbon tetrachloride; rat; liver injury
Restricted access

Vinpocetine is a widely used drug for the treatment of cerebrovascular and memory disorders. This study aimed to investigate the effect of vinpocetine on the acute hepatic injury caused in the rat by the administration of CCl 4in vivo . Vinpocetine (2.1, 4.2, 8.4 mg/kg) or silymarin (30 mg/kg) was given once daily orally simultaneously with CCl 4 and for 15 days thereafter. Liver damage was assessed by determining serum enzyme activities and hepatic histopathology. Stained sections were subjected to morphometric evaluation using computerized image analyzer. The results showed that vinpocetine administered to CCl 4 -treated rats decreased the elevated alanine aminotransferase (ALT) by 49.3, 58.1 and 63.6%, aspartate aminotransferase (AST) by 10.5, 22.6 and 27.2% and alkaline phosphatase (ALP) by 52.5, 59.6 and 64.9%, respectively, and in a dose-dependent manner. Meanwhile, silymarin reduced elevated ALT, AST and ALP levels by 53.1, 26.9 and 66%, respectively. Histological examination of liver specimens revealed a marked reduction in liver cell necrosis in vinpocetine and silymarin-treated rats compared with vehicle-treated CCl 4 -treated rats. Quantitative analysis of the area of damage showed 85.3% reduction in the area of damage after silymarin and 72.2, 78.9 and 82.6% reduction after vinpocetine treatment at 2.1, 4.2, 8.4 mg/kg, respectively. It is concluded that administration of vinpocetine in a model of CCl 4 -induced liver injury in rats reduced liver damage. The reduction obtained by 4.2 mg/kg of vinpocetine was similar to that obtained by 30 mg/kg silymarin. Therefore, it is suggested that vinpocetine might be a good pharmacological agent in the treatment of liver disease besides its neuroprotective effects.

  • Arif, A., Levine, R. A., Sanderson, S. O., Bank, L., Velu, R. P., Shah, A., Mahl, T. C., Gregory, D. H. (2003) Regression of fibrosis in chronic hepatitis C after therapy with interferon and ribavirin. Dig. Dis. Sci. 48 , 1425–1430.

    Gregory D. H. , 'Regression of fibrosis in chronic hepatitis C after therapy with interferon and ribavirin ' (2003 ) 48 Dig. Dis. Sci. : 1425 -1430 .

    • Search Google Scholar

  • Bacon, B. R. (2004) Managing hepatitis C. Am. J. Manag. Care 10 , S30–S40.

    Bacon B. R. , 'Managing hepatitis C ' (2004 ) 10 Am. J. Manag. Care : S30 -S40 .

  • Bauer, J. D. (1982) Clinical Laboratory Methods . The C. V. Mosby Co., St. Louis, Toronto, London.

    Bauer J. D. , '', in Clinical Laboratory Methods , (1982 ) -.

  • Bedossa, R. (1999) Natural history of liver fibrosis. Adv. Liver. Pathol. 32 , 292–293.

    Bedossa R. , 'Natural history of liver fibrosis ' (1999 ) 32 Adv. Liver. Pathol. : 292 -293 .

    • Search Google Scholar

  • Belfield, A., Goldberg, D. M. (1971) Revised assay for serum phenyl phosphatase activity using 4-amino-antipyrine. Enzyme 12 , 561–573.

    Goldberg D. M. , 'Revised assay for serum phenyl phosphatase activity using 4-amino-antipyrine ' (1971 ) 12 Enzyme : 561 -573 .

    • Search Google Scholar

  • Bonoczk, P., Gulyas, B., Adam-Vizi, V., Nemes, A., Karpati, E., Kiss, B., Kapas, M., Szantay, C., Koncz, I., Zelles, T., Vas, A. (2000) Role of sodium channel inhibition in neuroprotection: effect of vinpocetine. Brain Res. Bull. 53 , 245–254.

    Vas A. , 'Role of sodium channel inhibition in neuroprotection: effect of vinpocetine ' (2000 ) 53 Brain Res. Bull. : 245 -254 .

    • Search Google Scholar

  • Crowley, L. V. (1967) The Reitman-Frankel colorimetric transaminase procedure in suspected myocardial infarction. Clin. Chem. 13, 482–487.

    Crowley L. V. , 'The Reitman-Frankel colorimetric transaminase procedure in suspected myocardial infarction ' (1967 ) 13 Clin. Chem. : 482 -487 .

    • Search Google Scholar

  • Farghali, H., Kamenikova, L., Hynie, S., Kmonickova, E. (2000) Silymarin effects on intracellular calcuim and cytoxicity: a study in perfused rat hepatocytes after oxidative stress injury. Pharmacol. Res. 41 , 231–237.

    Kmonickova E. , 'Silymarin effects on intracellular calcuim and cytoxicity: a study in perfused rat hepatocytes after oxidative stress injury ' (2000 ) 41 Pharmacol. Res. : 231 -237 .

    • Search Google Scholar

  • Flora, K., Hahn, M., Rosen, H., Benner, K. (1998) Milk thistle ( Silybum marianum ) for the therapy of liver disease. Am. J. Gastroenterol. 93 , 139–143.

    Benner K. , 'Milk thistle (Silybum marianum) for the therapy of liver disease ' (1998 ) 93 Am. J. Gastroenterol. : 139 -143 .

    • Search Google Scholar

  • Fried, M. W. (2002) Side effects of therapy of hepatitis C and their management. Hepatology 36 , S237–S244.

    Fried M. W. , 'Side effects of therapy of hepatitis C and their management ' (2002 ) 36 Hepatology : S237 -S244 .

    • Search Google Scholar

  • Hadjiev, D. (2003) Asymptomatic ischemic cerebrovascular disorders and neuroprotection with vinpocetine. Ideggyógy. Szemle 56 , 166–172.

    Hadjiev D. , 'Asymptomatic ischemic cerebrovascular disorders and neuroprotection with vinpocetine ' (2003 ) 56 Ideggyógy. Szemle : 166 -172 .

    • Search Google Scholar

  • Hsu, C. T., Schichijo, K., Ito, M., Sekine, I. (1993) The effect of chemical sympathectomy on acute liver injury induced by carbon tetrachloride in spontaneously hypertensive rats. J. Auton. Nerv. Syst.43 , 91–96.

    Sekine I. , 'The effect of chemical sympathectomy on acute liver injury induced by carbon tetrachloride in spontaneously hypertensive rats ' (1993 ) 43 J. Auton. Nerv. Syst. : 91 -96 .

    • Search Google Scholar

  • Kiss, B., Karpati, E. (1996) A blockade of Na (+) channels can prevent some of the mechanisms involved in CCl 4 induced hepatotoxicity. Acta Pharm. Hung.66 , 213–224.

    Karpati E. , 'A blockade of Na (+) channels can prevent some of the mechanisms involved in CCl4 induced hepatotoxicity ' (1996 ) 66 Acta Pharm. Hung. : 213 -224 .

    • Search Google Scholar

  • Landon, E. J., Naukam, R. J., Rama-Sastry, B. V. (1986) Effects of calcium channel blocking agents on calcium and centrilobular necrosis in the liver of rats treated with hepatotoxic agents. Biochem. Pharmacol. 35 , 697–705.

    Rama-Sastry B. V. , 'Effects of calcium channel blocking agents on calcium and centrilobular necrosis in the liver of rats treated with hepatotoxic agents ' (1986 ) 35 Biochem. Pharmacol. : 697 -705 .

    • Search Google Scholar

  • Lillestll, I. K., Helle, K. B., Aardal, S. (1998) Relaxing effects of cyclic GMP and cyclic AMP-enhancing agents on the long-lasting contraction to endothelin-1 in the porcine coronary artery. Scand. J. Clin. Lab. Invest. 58 , 625–634.

    Aardal S. , 'Relaxing effects of cyclic GMP and cyclic AMP-enhancing agents on the long-lasting contraction to endothelin-1 in the porcine coronary artery ' (1998 ) 58 Scand. J. Clin. Lab. Invest. : 625 -634 .

    • Search Google Scholar

  • Maher, J. J. (2001) Interactions between hepatic stellate cells and the immune system. Semin. Liver Dis. 21 , 417–426.

    Maher J. J. , 'Interactions between hepatic stellate cells and the immune system ' (2001 ) 21 Semin. Liver Dis. : 417 -426 .

    • Search Google Scholar

  • Manns, M. P., McHutchison, J. G., Gordon, S. C. et al. (2001) Peginterferon alfa-2b plus ribavirin compared with interferon alfa-2b plus ribavirin for initial treatment of chronic hepatitis C: a randomised trial. Lancet 358 , 958–965.

    Gordon S. C. , 'Peginterferon alfa-2b plus ribavirin compared with interferon alfa-2b plus ribavirin for initial treatment of chronic hepatitis C: a randomised trial ' (2001 ) 358 Lancet : 958 -965 .

    • Search Google Scholar

  • Marcellin, P., Asselah, T., Boyer, N. (2002) Fibrosis and disease progression in hepatitis C. Hepatology 36 , S47–56.

    Boyer N. , 'Fibrosis and disease progression in hepatitis C ' (2002 ) 36 Hepatology : S47 -56 .

    • Search Google Scholar

  • McCaughan, G. W., George, J. (2004) Fibrosis progression in chronic hepatitis C virus infection. Gut 53 , 318–321.

    George J. , 'Fibrosis progression in chronic hepatitis C virus infection ' (2004 ) 53 Gut : 318 -321 .

    • Search Google Scholar

  • Muriel, P., Mourelle, M. (1990) Prevention by silymarin of membrane alterations in acute CCl4 liver damage. J. Appl. Toxicol. 10 , 275–279.

    Mourelle M. , 'Prevention by silymarin of membrane alterations in acute CCl4 liver damage ' (1990 ) 10 J. Appl. Toxicol. : 275 -279 .

    • Search Google Scholar

  • Nekrassov, V., Sitges, M. (2000) Vinpocetine protects from aminoglycoside antibiotic-induced hearing loss in guinea pig in vivo. Brain. Res. 868 , 222–229.

    Sitges M. , 'Vinpocetine protects from aminoglycoside antibiotic-induced hearing loss in guinea pig in vivo ' (2000 ) 868 Brain. Res. : 222 -229 .

    • Search Google Scholar

  • Paget, G. E., Barnes, J. M. (1964) In: Laurence, D. R., Bacharach, A. L. (eds) Evaluation of Drug Activities Pharmacometics . Academic Press, London & NewYork, pp. 1–135.

    Barnes J. M. , '', in Evaluation of Drug Activities Pharmacometics , (1964 ) -.

  • Pianko, S., McHutchison, J. G. (2000) Treatment of hepatitis C with interferon and ribavirin. J. Gastroenterol. Hepatol. 15 , 581–586.

    McHutchison J. G. , 'Treatment of hepatitis C with interferon and ribavirin ' (2000 ) 15 J. Gastroenterol. Hepatol. : 581 -586 .

    • Search Google Scholar

  • Romero, G., Lasheras, B., Sainz-Suberviola, L., Cenarruzabeitia, E. (1994) Protective effects of calcium channel blockers in carbon tetrachloride-induced liver toxicity. Life Sci. 55 , 981–990.

    Cenarruzabeitia E. , 'Protective effects of calcium channel blockers in carbon tetrachloride-induced liver toxicity ' (1994 ) 55 Life Sci. : 981 -990 .

    • Search Google Scholar

  • Sippel, H., Stauffert, I., Estler, C. J. (1993) Protective effect of various calcium antagonists against an experimentally induced calcium overload in isolated hepatocytes. Biochem. Pharmacol. 46 , 1937–1944.

    Estler C. J. , 'Protective effect of various calcium antagonists against an experimentally induced calcium overload in isolated hepatocytes ' (1993 ) 46 Biochem. Pharmacol. : 1937 -1944 .

    • Search Google Scholar

  • Sitges, M., Galvan, E., Nekrassov, V. (2005) Vinpocetine blockade of sodium channels inhibits the rise in sodium and calcium induced by 4-aminopyridine in synaptosomes. Neurochem. Int. 46 , 533–540.

    Nekrassov V. , 'Vinpocetine blockade of sodium channels inhibits the rise in sodium and calcium induced by 4-aminopyridine in synaptosomes ' (2005 ) 46 Neurochem. Int. : 533 -540 .

    • Search Google Scholar

  • Tanaka, N., Tanaka, K., Nagashima, Y., Kondo, M., Sekihara, H. (1999) Nitric oxide increases hepatic arterial blood flow in rats with carbon tetrachloride-induced acute hepatic injury. Gastroenterology 117 , 173–180.

    Sekihara H. , 'Nitric oxide increases hepatic arterial blood flow in rats with carbon tetrachloride-induced acute hepatic injury ' (1999 ) 117 Gastroenterology : 173 -180 .

    • Search Google Scholar

  • Szakács, T., Veres, Zs., Vereczkey, L. (2001) In vitro-in vivo correlation of the pharmakokinetics of vinpocetine. Pol. J. Pharmacol. 53 , 623–628.

    Vereczkey L. , 'In vitro-in vivo correlation of the pharmakokinetics of vinpocetine ' (2001 ) 53 Pol. J. Pharmacol. : 623 -628 .

    • Search Google Scholar

  • Vas, A., Gulyas, B., Szabo, Z., Bonoczk, P., Csiba, L., Kiss, B., Karpati, E., Panczel, G., Nagy, Z. (2002) Clinical and non-clinical investigations using positron emission tomography, near infrared spectroscopy and transcranial Doppler methods on the neuroprotective drug vinpocetine: a summary of evidences. J. Neurol. Sci. 203-204 , 259–262.

    Nagy Z. , 'Clinical and non-clinical investigations using positron emission tomography, near infrared spectroscopy and transcranial Doppler methods on the neuroprotective drug vinpocetine: a summary of evidences ' (2002 ) 203-204 J. Neurol. Sci. : 259 -262 .

    • Search Google Scholar

  • Wellington, K., Jarvis, B. (2001) Silymarin: a review of its clinical properties in the management of hepatic disorders. BioDrugs 15 , 465–489.

    Jarvis B. , 'Silymarin: a review of its clinical properties in the management of hepatic disorders ' (2001 ) 15 BioDrugs : 465 -489 .

    • Search Google Scholar

  • Wu, S. N. (2003) Large-conductance Ca 2+ -activated K + channels: physiological role and pharmacology. Curr. Med. Chem. 10 , 649–661.

    Wu S. N. , 'Large-conductance Ca2+-activated K+ channels: physiological role and pharmacology ' (2003 ) 10 Curr. Med. Chem. : 649 -661 .

    • Search Google Scholar
  • Collapse
  • Expand
Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X

Senior editors

Editor(s)-in-Chief: Elekes, Károly

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

Acta Biologica Hungarica
P.O. Box 35
H-8237 Tihany, Hungary
Phone: (36 87) 448 244 ext. 103
Fax: (36 87) 448 006
E-mail: elekes@tres.blki.hu

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture
  • Biology and Environmental Sciences
  • Elsevier BIOBASE
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Acta Biologica Hungarica
Language English
Size  
Year of
Foundation		 1950
Publication
Programme		 changed title
Volumes
per Year		  
Issues
per Year		  
Founder Magyar Tudományos Akadémia
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Apr 2024 13 1 0
May 2024 8 0 0
Jun 2024 17 0 0
Jul 2024 44 0 0
Aug 2024 24 0 0
Sep 2024 15 0 0
Oct 2024 6 1 1