Acta Biologica Hungarica
Volume/Issue:
Volume 59: Issue 2
Analysis of the applicability of molecular markers linked to the PVY extreme resistance gene Rysto , and the identification of new markers
Authors:
I. Cernák University of Pannonia Department of Plant Sciences and Biotechnology Deák F. u. 16 H-8360 Keszthely Hungary

Search for other papers by I. Cernák in
Current site
Google Scholar
PubMed Close
,
J. Taller University of Pannonia Department of Plant Sciences and Biotechnology Deák F. u. 16 H-8360 Keszthely Hungary

Search for other papers by J. Taller in
Current site
Google Scholar
PubMed Close
,
I. Wolf University of Pannonia Potato Research Center Deák F. u. 16 H-8360 Keszthely Hungary

Search for other papers by I. Wolf in
Current site
Google Scholar
PubMed Close
,
E. Fehér University of Pannonia Department of Plant Sciences and Biotechnology Deák F. u. 16 H-8360 Keszthely Hungary

Search for other papers by E. Fehér in
Current site
Google Scholar
PubMed Close
,
G. Babinszky University of Pannonia Department of Plant Sciences and Biotechnology Deák F. u. 16 H-8360 Keszthely Hungary

Search for other papers by G. Babinszky in
Current site
Google Scholar
PubMed Close
,
Z. Alföldi University of Pannonia Department of Plant Sciences and Biotechnology Deák F. u. 16 H-8360 Keszthely Hungary

Search for other papers by Z. Alföldi in
Current site
Google Scholar
PubMed Close
,
G. Csanádi Roche (Hungary) Ltd. Edison u 1 H-2040 Budaörs Hungary

Search for other papers by G. Csanádi in
Current site
Google Scholar
PubMed Close
, and
Z. Polgár University of Pannonia Potato Research Center Deák F. u. 16 H-8360 Keszthely Hungary

Search for other papers by Z. Polgár in
Current site
Google Scholar
PubMed Close
Pages:
195–203
Online Publication Date:
31 May 2008
Publication Date:
01 Jun 2008
Article Category:
Research Article
DOI:
https://doi.org/10.1556/abiol.59.2008.2.6
Keywords:
Potato; PVY; resistance; Ry sto ; markers
Restricted access

In this study molecular markers linked to the Rysto gene, which originates from the wild potato species Solanum stoloniferum and confers extreme resistance against PVY, were identified and the applicability of recently published Rysto markers was analyzed. Three RAPD markers covering a total distance of 8.60 cM were detected in this experiment. The closest of these markers was located 0.53 cM from the gene. From among the published markers only one had diagnostic value in the experimental plant material, and mapped 2.95 cM from the gene, on the side opposite the RAPD markers developed in the present study. All the markers analyzed were present in Solanum stoloniferum accessions, irrespective of their resistance, indicating that these sequences are linked to the locus and not exclusively to the dominant allele of the Rysto gene in the wild species. The inapplicability of several published markers indicates that the genetic background is decisive in this tetraploid and highly heterozygous species. This means that it may be necessary to develop markers from the breeding material itself, until the resistance gene is not cloned and cannot be used as a selection marker in marker-assisted selection.

  • Brigneti, G., Garcia-Mas, J., Baulcombe, D. C. (1997) Molecular mapping of the potato virus Y resistance gene Rysto in potato. Theor. Appl. Genet. 94 , 198–203.

    Baulcombe D. C. , 'Molecular mapping of the potato virus Y resistance gene Rysto in potato ' (1997 ) 94 Theor. Appl. Genet. : 198 -203 .

    • Search Google Scholar

  • Celebi-Toprak, F., Slack, S. A., Jahn, M. M. (2002) A new gene, Nytbr , for hypersensitivity to potato virus Y from Solanum tuberosum . Theor. Appl. Genet. 104 , 669–674.

    Jahn M. M. , 'A new gene, Nytbr, for hypersensitivity to potato virus Y from Solanum tuberosum ' (2002 ) 104 Theor. Appl. Genet. : 669 -674 .

    • Search Google Scholar

  • Cockerham, G. (1943) Potato breeding for virus resistance. Ann. Appl. Biol. 30 , 105–108.

    Cockerham G. , 'Potato breeding for virus resistance ' (1943 ) 30 Ann. Appl. Biol. : 105 -108 .

    • Search Google Scholar

  • Flis, B., Hennig, J., Strzelczyk-Żyta, D., Gebhardt, C., Marczewski, W. (2005) The Ry-fsto gene from Solanum stoloniferum for extreme resistant to Potato virus Y maps to potato chromosome XII and is diagnosed by PCR marker GP122 718 in PVY resistant potato cultivars. Mol. Breed, 15 , 95–101.

    Marczewski W. , 'The Ry-fsto gene from Solanum stoloniferum for extreme resistant to Potato virus Y maps to potato chromosome XII and is diagnosed by PCR marker GP122718 in PVY resistant potato cultivars ' (2005 ) 15 Mol. Breed : 95 -101 .

    • Search Google Scholar

  • Gebhardt C., Valkonen, J. P. T. (2001) Organization of genes controlling disease resistance in the potato genome. Annu. Rev. Phytopathol. 39 , 79–102.

    Valkonen J. P. T. , 'Organization of genes controlling disease resistance in the potato genome ' (2001 ) 39 Annu. Rev. Phytopathol. : 79 -102 .

    • Search Google Scholar

  • Hämäläinen, H., Sorri, V. A., Watanabe, K. N., Gebhardt, C., Valkonen, J. P. T. (1998) Molecular examination of a chromosome region that controls resistance to potato Y and A potyviruses in potato. Theor. Appl. Genet. 96 , 1036–1043.

    Valkonen J. P. T. , 'Molecular examination of a chromosome region that controls resistance to potato Y and A potyviruses in potato ' (1998 ) 96 Theor. Appl. Genet. : 1036 -1043 .

    • Search Google Scholar

  • Hämäläinen, J. H., Watanabe, K. N., Valkonen, J. P. T., Arihara, A., Plaisted, R. L., Pehu, E., Miller, L., Slack, S. A. (1997) Mapping and marker-assisted selection for a gene for extreme resistance to potato virus Y. Theor. Appl. Genet. 94 , 192–197.

    Slack S. A. , 'Mapping and marker-assisted selection for a gene for extreme resistance to potato virus Y ' (1997 ) 94 Theor. Appl. Genet. : 192 -197 .

    • Search Google Scholar

  • Hosaka, K., Hosaka, Y., Mori, M., Maida, T., Matsunaga, H. (2001) Detection of a simplex RAPD marker linked to resistance to potato virus Y in a tetraploid potato. Am. J. Potato Res. 78 , 191–196.

    Matsunaga H. , 'Detection of a simplex RAPD marker linked to resistance to potato virus Y in a tetraploid potato ' (2001 ) 78 Am. J. Potato Res. : 191 -196 .

    • Search Google Scholar

  • Kasai, K., Morikawa, Y., Sorri, V. A., Valkonen, J. P. T., Gebhardt, C., Watanabe, K. N. (2000) Development of SCAR markers to the PVY resistance gene Ryadg based on a common feature of plant disease resistance genes. Genome 43 , 1–8.

    Watanabe K. N. , 'Development of SCAR markers to the PVY resistance gene Ryadg based on a common feature of plant disease resistance genes ' (2000 ) 43 Genome : 1 -8 .

    • Search Google Scholar

  • Milbourne, D., Meyer, R. C., Collins, A. J., Ramsay, R. D., Gebhardt, C., Waugh, R. (1998) Isolation, characterisation and mapping of simple sequence repeat loci in potato. Mol. Gen. Genet. 259 , 233–245.

    Waugh R. , 'Isolation, characterisation and mapping of simple sequence repeat loci in potato ' (1998 ) 259 Mol. Gen. Genet. : 233 -245 .

    • Search Google Scholar

  • Ross, H. (1986) Potato Breeding — Problems and Perspectives . Paul Parey, Berlin & Hamburg.

    Ross H. , '', in Potato Breeding — Problems and Perspectives , (1986 ) -.

  • Solomon-Blackburn, R. M., Barker, H. (2001) A review of host major-gene resistance to potato viruses X, Y, A and V in potato genes, genetics and mapped locations. Heredity 86 , 8–16.

    Barker H. , 'A review of host major-gene resistance to potato viruses X, Y, A and V in potato genes, genetics and mapped locations ' (2001 ) 86 Heredity : 8 -16 .

    • Search Google Scholar

  • Song, Y. S. (2004) Genetic marker analysis in potato for extreme resistance ( Rysto ) to PVY and for chip quality after long term storage at 4 °C. PhD Dissertation: University of Technology, München, Germany.

    Song Y. S. , '', in Genetic marker analysis in potato for extreme resistance (Rysto) to PVY and for chip quality after long term storage at 4 °C , (2004 ) -.

  • Song, Y. S., Hepting, L., Schweizer, G., Hartl, L., Wenzel, G., Schwarzfischer, A. (2005) Mapping of extreme resistance to PVY ( Rysto ) on chromosome XII using anther-culture-derived primary dihaploid potato lines. Theor. Appl. Genet. 111 , 879–887.

    Schwarzfischer A. , 'Mapping of extreme resistance to PVY (Rysto) on chromosome XII using anther-culture-derived primary dihaploid potato lines ' (2005 ) 111 Theor. Appl. Genet. : 879 -887 .

    • Search Google Scholar

  • Takács, A., Kazinczi, G., Horváth, J., Bősze, Z., Pribék, D. (1999) Resistance of new wild Solanum species to NTN strain of potato Y potyvirus (PVY NTN ). Meded. Fac. Landbouww. Univ. Gent 64/3b , 513–520.

    Pribék D. , 'Resistance of new wild Solanum species to NTN strain of potato Y potyvirus (PVYNTN) ' (1999 ) 64/3b Meded. Fac. Landbouww. Univ. Gent : 513 -520 .

    • Search Google Scholar

  • Walbot, V., Warren, C. (1988) Regulation of Mu element copy number in maize line with an active or inactive mutator transposable element system. Mol. Gen. Genet. 211 , 27–34.

    Warren C. , 'Regulation of Mu element copy number in maize line with an active or inactive mutator transposable element system ' (1988 ) 211 Mol. Gen. Genet. : 27 -34 .

    • Search Google Scholar
  • Collapse
  • Expand
Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X

Senior editors

Editor(s)-in-Chief: Elekes, Károly

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

Acta Biologica Hungarica
P.O. Box 35
H-8237 Tihany, Hungary
Phone: (36 87) 448 244 ext. 103
Fax: (36 87) 448 006
E-mail: elekes@tres.blki.hu

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture
  • Biology and Environmental Sciences
  • Elsevier BIOBASE
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Acta Biologica Hungarica
Language English
Size  
Year of
Foundation		 1950
Publication
Programme		 changed title
Volumes
per Year		  
Issues
per Year		  
Founder Magyar Tudományos Akadémia
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Nov 2024 34 0 0
Dec 2024 49 0 0
Jan 2025 48 0 0
Feb 2025 56 0 0
Mar 2025 73 2 1
Apr 2025 31 7 7
May 2025 4 1 1