Acta Biologica Hungarica
Volume/Issue:
Volume 59: Issue 2
Effect of copper excess on H 2 O 2 accumulation and peroxidase activities in bean roots
Authors:
Houda Bouazizi Faculté des Sciences de Bizerte Laboratoire de Bio-Physiologie Cellulaires 7021 Zarzouna Tunisia

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Hager Jouili Faculté des Sciences de Bizerte Laboratoire de Bio-Physiologie Cellulaires 7021 Zarzouna Tunisia

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Anja Geitmann Institut de recherche en biologie végétale Laboratoire de Microscopie 4101 rue Sherbrooke Montréal Québec H1X 2B2 Canada

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E. Ferjani Faculté des Sciences de Bizerte Laboratoire de Bio-Physiologie Cellulaires 7021 Zarzouna Tunisia

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Pages:
233–245
Online Publication Date:
31 May 2008
Publication Date:
01 Jun 2008
Article Category:
Research Article
DOI:
https://doi.org/10.1556/abiol.59.2008.2.9
Keywords:
Bean; peroxidase; H 2 O 2; cell wall; copper
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We studied oxidative stress and peroxidase activity resulting from application of excess copper in the nutrient medium on the roots of young bean seedlings. The change in H 2 O 2 content, lipid peroxidation and antioxidant enzymes activities were quantified and located. Excess of copper caused a loss of membrane integrity and the formation of hydrogen peroxide (H 2 O 2 ) as visualized in the transmission electron microscopy and measured using spectrophotometry. H 2 O 2 accumulated in the intercellular spaces and in the cell wall. The production of H 2 O 2 was accompanied by an increase in the activity of soluble and ionic GPX (guaiacol peroxidase, EC 1.11.17), CAPX (coniferyl alcohol peroxidase) and NADH oxidase.

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Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X

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Editor(s)-in-Chief: Elekes, Károly

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    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
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    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
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    1. Kása, Péter (Szeged)
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    1. Roberts, D. F. (Newcastle-upon-Tyne)
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    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

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Acta Biologica Hungarica
Language English
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Foundation		 1950
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Founder Magyar Tudományos Akadémia
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