Acta Biologica Hungarica
Volume/Issue:
Volume 59: Issue 4
Rat testicular mitochondrial antioxidant defence system and its modulation by aging
Authors:
D. Sahoo Utkal University Department of Zoology Bhubaneswar, Orissa 751004 India

Search for other papers by D. Sahoo in
Current site
Google Scholar
PubMed Close
,
Anita Roy Utkal University Department of Zoology Bhubaneswar, Orissa 751004 India

Search for other papers by Anita Roy in
Current site
Google Scholar
PubMed Close
, and
G. Chainy

Search for other papers by G. Chainy in
Current site
Google Scholar
PubMed Close
Pages:
413–424
Online Publication Date:
10 Dec 2008
Publication Date:
01 Dec 2008
Article Category:
Research Article
DOI:
https://doi.org/10.1556/abiol.59.2008.4.3
Keywords:
Aging; rat; testes; mitochondria; reactive oxygen species; oxidative stress
Restricted access

Accumulation of oxidative damage caused by reactive oxygen species (ROS) underlies fundamental changes found during aging. In the present study, age related effect on testicular mitochondrial oxidant generation and antioxidant defence profile was investigated in Wistar rats at 3 months (young adults), 12 months (old adults) and 24 months (senescent animals) of age. Mitochondrial oxidative stress parameters viz., lipid peroxidation (LPx), protein carbonylation, hydrogen peroxide (H 2 O 2 ) generation and activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), levels of total, oxidized (GSSG) and reduced glutathione (GSH) were studied to find out their roles in maintenance of mitochondrial glutathione redox pool as a function of age. Increased levels of LPx, H 2 O 2 and decreased GSH content accompanied by a decline in activities of SOD, GPx and GR with advancing age suggest that antioxidant defense profile of testicular mitochondria exhibit age related alterations which might play a critical role in regulating physiological functions of the testis such as steroidogenesis and spermatogenesis.

  • Cao, L., Leers-Sucheta, S., Azhar, S. (2004) Aging alters the functional expression of enzymatic and non-enzymatic antioxidant defence system in testicular rat Leydig cells. J. Steroid Biochem. Mol. Biol. 88 , 61–70.

    Azhar S. , 'Aging alters the functional expression of enzymatic and non-enzymatic antioxidant defence system in testicular rat Leydig cells ' (2004 ) 88 J. Steroid Biochem. Mol. Biol. : 61 -70 .

    • Search Google Scholar

  • Capel, F., Rimbert, V., Lioger, D., Diot, A., Rousset, P., Mirand, P. P., Boirie, Y., Morio, B., Mosoni, L. (2005) Due to reverse electron transfer, mitochondrial H 2 O 2 release increases with age in human vastus lateralis muscle although oxidative capacity is preserved. Mech. Ageing Dev. 126 , 505–511.

    Mosoni L. , 'Due to reverse electron transfer, mitochondrial H2O2 release increases with age in human vastus lateralis muscle although oxidative capacity is preserved ' (2005 ) 126 Mech. Ageing Dev. : 505 -511 .

    • Search Google Scholar

  • Carmagnol, F., Sinet, P. M., Jerome, H. (1983) Selenium-dependent and non-selenium-dependent glutathione peroxidases in human tissue extracts. Biochim. Biophys. Acta 759 , 49–57.

    Jerome H. , 'Selenium-dependent and non-selenium-dependent glutathione peroxidases in human tissue extracts ' (1983 ) 759 Biochim. Biophys. Acta : 49 -57 .

    • Search Google Scholar

  • Chen, J., Irizarry, R. A., Luo, L., Zirkin, B. R. (2004) Leydig cells gene expression: Effect of age and caloric restriction. Expt. Gerontol. 39 , 31–40.

    Zirkin B. R. , 'Leydig cells gene expression: Effect of age and caloric restriction ' (2004 ) 39 Expt. Gerontol. : 31 -40 .

    • Search Google Scholar

  • Cohen, G., Dembiec, D., Marcus, J. (1970) Measurement of catalase activity in tissue extracts. Anal. Biochem. 34 , 30–38.

    Marcus J. , 'Measurement of catalase activity in tissue extracts ' (1970 ) 34 Anal. Biochem. : 30 -38 .

    • Search Google Scholar

  • Das, K., Samanta, L., Chainy, G. B. N. (2000) A modified spectrophotometric assay of superoxide dismutase using nitrite formation of superoxide radicals. Ind. J. Biochem. Biophys. 37 , 201–204.

    Chainy G. B. N. , 'A modified spectrophotometric assay of superoxide dismutase using nitrite formation of superoxide radicals ' (2000 ) 37 Ind. J. Biochem. Biophys. : 201 -204 .

    • Search Google Scholar

  • de la Asuncion, J. G., Millan, A., Pla, R., Bruseghini, L., Esteras, A., Pallardo, F. V., Sastre, J., Vina, J. (1996) Mitochondrial glutathione oxidation correlates with age-associated oxidative damage to mitochondrial DNA. FASEB J. 10 , 333–338.

    Vina J. , 'Mitochondrial glutathione oxidation correlates with age-associated oxidative damage to mitochondrial DNA ' (1996 ) 10 FASEB J. : 333 -338 .

    • Search Google Scholar

  • Fucci, L., Oliver, C. N., Coon, M. J., Stadtman, E. R. (1983) Inactivation of key metabolic enzymes by mixed function oxidation reactions: possible implication in protein turnover and aging. Proc. Nat. Acad. Sci. USA 80 , 1521–1525.

    Stadtman E. R. , 'Inactivation of key metabolic enzymes by mixed function oxidation reactions: possible implication in protein turnover and aging ' (1983 ) 80 Proc. Nat. Acad. Sci. USA : 1521 -1525 .

    • Search Google Scholar

  • Harman, D. (1956) Aging: a theory based on free radical and radiation chemistry. J. Gerontol. 11 , 298–300.

    Harman D. , 'Aging: a theory based on free radical and radiation chemistry ' (1956 ) 11 J. Gerontol. : 298 -300 .

    • Search Google Scholar

  • Kaur, A. (2005) Changes in peroxidase activity and structure of testes, epididymis and seminal vesicles in aging Swiss albino mice. Ind. J. Gerontol. 19 , 119–126.

    Kaur A. , 'Changes in peroxidase activity and structure of testes, epididymis and seminal vesicles in aging Swiss albino mice ' (2005 ) 19 Ind. J. Gerontol. : 119 -126 .

    • Search Google Scholar

  • Kumaran, S., Deepak, B., Naveen, B., Panneerselvam, C. (2003) Effects of levocarnitine on mitochondrial antioxidant systems and oxidative stress in aged rats. Drugs R.D. 4 , 141–147.

    Panneerselvam C. , 'Effects of levocarnitine on mitochondrial antioxidant systems and oxidative stress in aged rats ' (2003 ) 4 Drugs R.D. : 141 -147 .

    • Search Google Scholar

  • Lee, C. Y. (1982) Glucose-6-phosphate dehydrogenase from mouse. Methods Enzymol. 89 , 252–260.

    Lee C. Y. , 'Glucose-6-phosphate dehydrogenase from mouse ' (1982 ) 89 Methods Enzymol. : 252 -260 .

    • Search Google Scholar

  • Lee, H. C., Wei, Y. H. (2000) Mitochondrial role in life and death of the cell. J. Biomed. Sci. 7 , 2–15.

    Wei Y. H. , 'Mitochondrial role in life and death of the cell ' (2000 ) 7 J. Biomed. Sci. : 2 -15 .

    • Search Google Scholar

  • Levine, R. L., Garland, D., Oliver, C. N., Amici, A., Climent, I., Lenz, A. G., Ahn, B. W., Shaltiel, S., Stadtman, E. R. (1990) Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol. 186 , 464–478.

    Stadtman E. R. , 'Determination of carbonyl content in oxidatively modified proteins ' (1990 ) 186 Methods Enzymol. : 464 -478 .

    • Search Google Scholar

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J. (1951) Protein measurement with the Folin-phenol reagent. J. Biol. Chem. 193 , 265–275.

    Randall R. J. , 'Protein measurement with the Folin-phenol reagent ' (1951 ) 193 J. Biol. Chem. : 265 -275 .

    • Search Google Scholar

  • Luo, L., Chen, H., Trush, M. A., Show, M. D., Anway, M. D., Zirkin, B. R. (2006) Aging and the brown Norway rat Leydig cell antioxidant defence system. J. Androl. 27 , 240–247.

    Zirkin B. R. , 'Aging and the brown Norway rat Leydig cell antioxidant defence system ' (2006 ) 27 J. Androl. : 240 -247 .

    • Search Google Scholar

  • Massey, V., Williams, C. H. (1965) On the reaction mechanism of yeast glutathione reductase. J. Biol. Chem. 240 , 4470–4481.

    Williams C. H. , 'On the reaction mechanism of yeast glutathione reductase ' (1965 ) 240 J. Biol. Chem. : 4470 -4481 .

    • Search Google Scholar

  • Matsubara, L. S., Machado, P. E. A. (1991) Age related changes of glutathione content, glutathione reductase and glutathione peroxidase activity of human erythrocytes. Brazil. J. Med. Biol. Res. 24 , 449–459.

    Machado P. E. A. , 'Age related changes of glutathione content, glutathione reductase and glutathione peroxidase activity of human erythrocytes ' (1991 ) 24 Brazil. J. Med. Biol. Res. : 449 -459 .

    • Search Google Scholar

  • Miquel, J., Economos, A. C., Fleming, J., Johnson, J. E. Jr. (1980) Mitochondrial role in cell aging. Exp. Gerontol. 15 , 579–591.

    Johnson J. E. , 'Mitochondrial role in cell aging ' (1980 ) 15 Exp. Gerontol. : 579 -591 .

    • Search Google Scholar

  • Nagley, P., Wei, Y. H. (1998) Ageing and mammalian mitochondrial genetics. Trends Genet. 14 , 513–517.

    Wei Y. H. , 'Ageing and mammalian mitochondrial genetics ' (1998 ) 14 Trends Genet. : 513 -517 .

    • Search Google Scholar

  • Navarro, A., Boveris, A. (2004) Rat brain and liver mitochondria develop oxidative stress and lose enzymatic activities on aging. Am. J. Physiol. Regul. Integr. Comp. Physiol. 287 , R1244–R1249.

    Boveris A. , 'Rat brain and liver mitochondria develop oxidative stress and lose enzymatic activities on aging ' (2004 ) 287 Am. J. Physiol. Regul. Integr. Comp. Physiol. : R1244 -R1249 .

    • Search Google Scholar

  • Ohkawa, H., Ohishi, N., Yagi, K. (1979) Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal. Biochem. 95 , 351–358.

    Yagi K. , 'Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction ' (1979 ) 95 Anal. Biochem. : 351 -358 .

    • Search Google Scholar

  • Paglia, D. E., Valentine, W. N. (1967) Studies on quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med. 70 , 158–169.

    Valentine W. N. , 'Studies on quantitative and qualitative characterization of erythrocyte glutathione peroxidase ' (1967 ) 70 J. Lab. Clin. Med. : 158 -169 .

    • Search Google Scholar

  • Peltola, V., Huhtaniemi, I., Ahotupa, M. (1992) Antioxidant enzyme activity in the maturing rat testis. J. Androl. 13 , 450–455.

    Ahotupa M. , 'Antioxidant enzyme activity in the maturing rat testis ' (1992 ) 13 J. Androl. : 450 -455 .

    • Search Google Scholar

  • Pick, E., Keisari, Y. (1981) Superoxide anion and hydrogen peroxide production by chemically elicited peritoneal macrophages-induction by multiple nonphagocytic stimuli. Cell. Immunol. 59 , 301–318.

    Keisari Y. , 'Superoxide anion and hydrogen peroxide production by chemically elicited peritoneal macrophages-induction by multiple nonphagocytic stimuli ' (1981 ) 59 Cell. Immunol. : 301 -318 .

    • Search Google Scholar

  • Sahoo, D. K., Roy, A., Bhanja, S., Chainy, G. B. N. (2005) Experimental hyperthyroidism-induced oxidative stress and impairment of antioxidant defence system in rat testis. Ind. J. Exp. Biol. 43 , 1058–1067.

    Chainy G. B. N. , 'Experimental hyperthyroidism-induced oxidative stress and impairment of antioxidant defence system in rat testis ' (2005 ) 43 Ind. J. Exp. Biol. : 1058 -1067 .

    • Search Google Scholar

  • Sahoo, D. K., Roy, A., Bhanja, S., Chainy, G. B. N. (2008) Hypothyroidism impairs antioxidant defence system and testicular physiology during development and maturation. Gen. Comp. Endocrinol. 156 , 63–70.

    Chainy G. B. N. , 'Hypothyroidism impairs antioxidant defence system and testicular physiology during development and maturation ' (2008 ) 156 Gen. Comp. Endocrinol. : 63 -70 .

    • Search Google Scholar

  • Sahoo, D. K., Roy, A., Chattopadhyay, S., Chainy, G. B. N. (2007) Effect of T 3 treatment on glutathione redox pool and its metabolizing enzymes in mitochondrial and post-mitochondrial fractions of adult rat testes. Ind. J. Exp. Biol. 45 , 338–346.

    Chainy G. B. N. , 'Effect of T3 treatment on glutathione redox pool and its metabolizing enzymes in mitochondrial and post-mitochondrial fractions of adult rat testes ' (2007 ) 45 Ind. J. Exp. Biol. : 338 -346 .

    • Search Google Scholar

  • Samanta, L., Chainy, G. B. N. (1997) Comparison of hexachlorocyclohexane-induced oxidative stress in the testis of immature and adult rats. Comp. Biochem. Physiol. 118 , 319–327.

    Chainy G. B. N. , 'Comparison of hexachlorocyclohexane-induced oxidative stress in the testis of immature and adult rats ' (1997 ) 118 Comp. Biochem. Physiol. : 319 -327 .

    • Search Google Scholar

  • Samanta, L., Chainy, G. B. N. (2002) Effect of age on testicular antioxidant defence system. Ind. J. Gerontol. 16 , 69–78.

    Chainy G. B. N. , 'Effect of age on testicular antioxidant defence system ' (2002 ) 16 Ind. J. Gerontol. : 69 -78 .

    • Search Google Scholar

  • Samanta, L., Roy, A., Chainy, G. B. N. (1999) Changes in rat testicular antioxidant defence profile as a function of age and its impairment by hexachlorocyclohexane during critical stages of maturation. Andrologia 31 , 83–90.

    Chainy G. B. N. , 'Changes in rat testicular antioxidant defence profile as a function of age and its impairment by hexachlorocyclohexane during critical stages of maturation ' (1999 ) 31 Andrologia : 83 -90 .

    • Search Google Scholar

  • Sastre, J., Pallardo, F. V., Vina, J. (2003) The role of mitochondrial oxidative stress in aging. Free Rad. Biol. Med. 35 , 1–8.

    Vina J. , 'The role of mitochondrial oxidative stress in aging ' (2003 ) 35 Free Rad. Biol. Med. : 1 -8 .

    • Search Google Scholar

  • Sharma, R., Nakamura, A., Takahashi, R., Nakamoto, H., Goto, S. (2006) Carbonyl modification in rat liver histones: Decrease with age and increase by dietary restriction. Free Rad. Biol. Med. 40 , 1179–1184.

    Goto S. , 'Carbonyl modification in rat liver histones: Decrease with age and increase by dietary restriction ' (2006 ) 40 Free Rad. Biol. Med. : 1179 -1184 .

    • Search Google Scholar

  • Shigenaga, M. K., Hagen, T. M., Ames, B. N. (1994) Oxidative damage and mitochondrial decay in aging. Proc. Natl. Acad. Sci. USA 91 , 10771–10778.

    Ames B. N. , 'Oxidative damage and mitochondrial decay in aging ' (1994 ) 91 Proc. Natl. Acad. Sci. USA : 10771 -10778 .

    • Search Google Scholar

  • Sohal, R. S. (1993) The free radical hypothesis of aging: an appraisal of the current status. Aging Clin. Exp. Res. 5 , 3–17.

    Sohal R. S. , 'The free radical hypothesis of aging: an appraisal of the current status ' (1993 ) 5 Aging Clin. Exp. Res. : 3 -17 .

    • Search Google Scholar

  • Sohal, R. S., Arnold, L. A., Sohal, B. H. (1990) Age related changes in antioxidant enzymes and prooxidant generation in tissues of the rat with special reference to parameters in two insect species. Free Radic. Biol. Med. 10 , 495–500.

    Sohal B. H. , 'Age related changes in antioxidant enzymes and prooxidant generation in tissues of the rat with special reference to parameters in two insect species ' (1990 ) 10 Free Radic. Biol. Med. : 495 -500 .

    • Search Google Scholar

  • Teaf, C. M., Harbison, R. D., Bishop, J. B. (1985) Germ-cell mutagenesis and GSH depression in reproductive tissue of the F-344 rat induced by ethyl methanesulfonate. Mut. Res. 144 , 93–98.

    Bishop J. B. , 'Germ-cell mutagenesis and GSH depression in reproductive tissue of the F-344 rat induced by ethyl methanesulfonate ' (1985 ) 144 Mut. Res. : 93 -98 .

    • Search Google Scholar

  • Zirkin, B. R., Chen, H. (2000) Regulation of leydig cell steroidogenic function during aging. Biol. Reprod. 63 , 977–981.

    Chen H. , 'Regulation of leydig cell steroidogenic function during aging ' (2000 ) 63 Biol. Reprod. : 977 -981 .

    • Search Google Scholar
  • Collapse
  • Expand
Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X

Senior editors

Editor(s)-in-Chief: Elekes, Károly

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

Acta Biologica Hungarica
P.O. Box 35
H-8237 Tihany, Hungary
Phone: (36 87) 448 244 ext. 103
Fax: (36 87) 448 006
E-mail: elekes@tres.blki.hu

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture
  • Biology and Environmental Sciences
  • Elsevier BIOBASE
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Acta Biologica Hungarica
Language English
Size  
Year of
Foundation		 1950
Publication
Programme		 changed title
Volumes
per Year		  
Issues
per Year		  
Founder Magyar Tudományos Akadémia
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Mar 2024 16 0 0
Apr 2024 4 0 0
May 2024 28 0 0
Jun 2024 25 0 0
Jul 2024 48 0 0
Aug 2024 39 0 0
Sep 2024 8 0 0