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  • 1 Genetic Engineering and Biotechnology Institute (GEBI) The Scientific and Technological Research Council of Turkey (TUBITAK), Marmara Research Center (MRC) P.O. Box 21 41470 Gebze, Kocaeli Turkey
  • | 2 Marmara University Department of Biology, Faculty of Science and Art 34722 Kadikoy, Istanbul Turkey
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Effects of salt stress on root growth, mitotic index, nuclear volume, vacuolization, nucleolar distortion and starch content were investigated in Turkish bread wheat ( Triticum aestivum L. cvs. Yildiz — salt sensitive, Dagdas — salt tolerant) and durum wheat ( Triticum durum L. cvs. C1252 — salt sensitive, Meramsalt tolerant) genotypes which were treated with 150 mM NaCI over a 6-day period. Salt treatment of wheat seedlings resulted in a decrease in root elongation and cell division in all genotypes at the 48 hours. According to controls, wheat root length decrease was 49% for Dagdas, 53.34% for Yildiz, 25.34% for Meram, 53.68% for C1252 at the 48 h. Mitotic index showed a more significant decrease in sensitive genotypes (1.24% for Yildiz, 0.66% for C1252 compairing to their controls 3.85% and 3.72%, respectively) of bread and durum wheat rather than tolerant ones (2.21% for Dagdas, 1.57% for Meram compairing to their controls 4.12% and 5.88%, respectively) at the 48 h of salt treatment. Calculated nuclear volume of wheat genotypes besides Dagdas showed a decline at the 48 h ranged from 1.57×10 5 to 2.13×10 5 μm 3 . Vacuolization and nuclear distortion appeared on DAPI-stained preparations. There was a clear reduction in starch content in salt treated genotypes of durum wheat.

  • Ashraf, M., O’leary, J. M. (1997) Ion distribution in leaves of salt-tolerant and salt-sensitive lines of spring wheat under salt stress. Acta Bot. Neerl. 46 , 207–217.

    O’leary J. M. , 'Ion distribution in leaves of salt-tolerant and salt-sensitive lines of spring wheat under salt stress ' (1997 ) 46 Acta Bot. Neerl. : 207 -217.

    • Search Google Scholar
  • de Agazio M., Grego, S., Ciofi-Luzzatto A., Rea, E., Zaccaria, M. L., Federico, R. (1995) Inhibition of maize primary root elongation by spermidine: effect on cell shape and mitotic index. J. Plant Growth Regul. 14 , 85–89.

    Federico R. , 'Inhibition of maize primary root elongation by spermidine: effect on cell shape and mitotic index ' (1995 ) 14 J. Plant Growth Regul. : 85 -89.

    • Search Google Scholar
  • Dodd, G. L., Donovan, L. A. (1999) Water potential and ionic effects of germination and seedling growth of two cold desert shrubs. Amer. Jour. Botan. 86 , 1146–1153.

    Donovan L. A. , 'Water potential and ionic effects of germination and seedling growth of two cold desert shrubs ' (1999 ) 86 Amer. Jour. Botan. : 1146 -1153.

    • Search Google Scholar
  • El-Mashad, A. A., Kamel, E. A. (2001) Amelioration of NaCl stress in Pisum sativum Linn. Indian J. Exp. Biol. 39 , 469–475.

    Kamel E. A. , 'Amelioration of NaCl stress in Pisum sativum Linn ' (2001 ) 39 Indian J. Exp. Biol. : 469 -475.

    • Search Google Scholar
  • Greenway, H., Munns, R. (1980) Mechanisms of salt tolerance in nonhalophytes. Annu. Rev. Plant Physiol. 31 , 141–190.

    Munns R. , 'Mechanisms of salt tolerance in nonhalophytes ' (1980 ) 31 Annu. Rev. Plant Physiol. : 141 -190.

    • Search Google Scholar
  • Hasegawa, P. M., Bressan, R. A., Zhu, J.-K., Bohnert, H. J. (2000) Plant cellular and molecular responses to high salinity. Annu. Rev. Plant Physiol. Plant Mol. Biol. 51 , 463–499.

    Bohnert H. J. , 'Plant cellular and molecular responses to high salinity ' (2000 ) 51 Annu. Rev. Plant Physiol. Plant Mol. Biol. : 463 -499.

    • Search Google Scholar
  • Hoagland, D. R., Arnon, D. I. (1950) The water culture method for plants without soil , Circ. 347. Berkeley, CA: California Agricultural Experiment Station.

    Arnon D. I. , '', in The water culture method for plants without soil , (1950 ) -.

  • Juliano, B. O. (1980) Properties of the rice caryopsis. In: Luh, B. S., Wesport, C. N. (eds) Rice production and utilization. AVI Publishing Co. Inc., pp. 201–208.

  • Kafi, M., Stewart, W. S., Borland, A. M. (2003) Carbohydrate and proline contents in leaves, roots, and apices of salt-tolerant and salt-sensitive wheat cultivars. Russ. J. Plant Physiol. 50 , 155–162.

    Borland A. M. , 'Carbohydrate and proline contents in leaves, roots, and apices of salt-tolerant and salt-sensitive wheat cultivars ' (2003 ) 50 Russ. J. Plant Physiol. : 155 -162.

    • Search Google Scholar
  • Katsuhara, M., Kawasaki, T. (1996) Salt stress induced nuclear and DNA degradation in meristematic cells of barley roots. Plant Cell Physiol . 37 , 169–173.

    Kawasaki T. , 'Salt stress induced nuclear and DNA degradation in meristematic cells of barley roots ' (1996 ) 37 Plant Cell Physiol : 169 -173.

    • Search Google Scholar
  • Munns, R., Termaat, A. (1986) Whole-plant responses to salinity. Aust. J. Plant Physiol. 13 , 143–160.

    Termaat A. , 'Whole-plant responses to salinity ' (1986 ) 13 Aust. J. Plant Physiol. : 143 -160.

    • Search Google Scholar
  • Murillo-Amador, B., Lopez-Aguliar, R., Kaya, C., Larrinaga-Mayoral, J., Flores-Hernandez, A. (2002) Comparative effects of NaCI and polyethylene glycol on germination, emergence and seedling growth of cowpea. J. Agricul. Crop Sci. 188 , 235–247.

    Flores-Hernandez A. , 'Comparative effects of NaCI and polyethylene glycol on germination, emergence and seedling growth of cowpea ' (2002 ) 188 J. Agricul. Crop Sci. : 235 -247.

    • Search Google Scholar
  • Ogawa, A., Kitamichi, K., Toyofuku, K., Kawashima, C. (2006) Quantitative analysis of cell division and cell death in seminal root of rye under salt stress. Plant Prod. Sci. 91 , 56–64.

    Kawashima C. , 'Quantitative analysis of cell division and cell death in seminal root of rye under salt stress ' (2006 ) 91 Plant Prod. Sci. : 56 -64.

    • Search Google Scholar
  • Poustini, K. (1990) Salinity and Translocation of Carbohydrates to Growing Grains, PhD Thesis, University of London.

  • Rathert, G. (1984) Sucrose and starch content of plant parts as a possible indicator for salt tolerance of crops. Aust. J. Plant Physiol. 11 , 491–495.

    Rathert G. , 'Sucrose and starch content of plant parts as a possible indicator for salt tolerance of crops ' (1984 ) 11 Aust. J. Plant Physiol. : 491 -495.

    • Search Google Scholar
  • Rubinigg, M., Wenisch, J., Elzenga, J. T. M., Stulen, I. (2004) NaCl salinity effects lateral root development in Plantago maritima . Fun. Plant Bio. 31 , 775–780.

    Stulen I. , 'NaCl salinity effects lateral root development in Plantago maritima ' (2004 ) 31 Fun. Plant Bio. : 775 -780.

    • Search Google Scholar
  • Sacks, M. M., Silk, W. K., Burman, P. (1997) Effect of water stress on cortical cell division rates within the apical meristem of primary roots of maize. Plant Physiol. 114 , 519–527.

    Burman P. , 'Effect of water stress on cortical cell division rates within the apical meristem of primary roots of maize ' (1997 ) 114 Plant Physiol. : 519 -527.

    • Search Google Scholar
  • Samarajeewa, P. K., Barrero, R. A., Umeda-Hara, C., Kawai, M., Uchimiya, H. (1999) Cortical cell death, cell proliferation, macromolecular movements and rTip1 expression patterns in roots of rice ( Oryza sativa L.) under NaCl stress. Planta 207 , 354–361.

    Uchimiya H. , 'Cortical cell death, cell proliferation, macromolecular movements and rTip1 expression patterns in roots of rice (Oryza sativa L.) under NaCl stress ' (1999 ) 207 Planta : 354 -361.

    • Search Google Scholar
  • Unal, M., Palavan-Unsal, N., Ismailoglu, I. (2005) The effect of putrescine and difluoromethylornithine on cell division activity of wheat in different ploidy level. Caryologia 58 , 15–20.

    Ismailoglu I. , 'The effect of putrescine and difluoromethylornithine on cell division activity of wheat in different ploidy level ' (2005 ) 58 Caryologia : 15 -20.

    • Search Google Scholar
  • Werker, E., Lerner, H. R., Weimberg, R., Poljakoff-Mayber, A. (1983) Structural changes occurring in nuclei of barley root cells in response to a combined effect of salinity and ageing. Amer. J. Bot. 70 , 222–225.

    Poljakoff-Mayber A. , 'Structural changes occurring in nuclei of barley root cells in response to a combined effect of salinity and ageing ' (1983 ) 70 Amer. J. Bot. : 222 -225.

    • Search Google Scholar
  • West, G., Inzé, D., Beemster, G. T. S. (2004) Cell cycle modulation in the response of the primary root of Arabidopsis to salt stress. Plant Physiol . 135 , 1050–1058.

    Beemster G. T. S. , 'Cell cycle modulation in the response of the primary root of Arabidopsis to salt stress ' (2004 ) 135 Plant Physiol : 1050 -1058.

    • Search Google Scholar
  • Yumurtaci, A., Vardar, F., Unal, M. (2007) Inhibition of barley root growth by actinomycin D: Effects on mitotic activity, protein content and peroxidase activity. Fresenius Environmental Bulletin 16 , 917–921.

    Unal M. , 'Inhibition of barley root growth by actinomycin D: Effects on mitotic activity, protein content and peroxidase activity ' (2007 ) 16 Fresenius Environmental Bulletin : 917 -921.

    • Search Google Scholar