Whole effluent toxicity is most often considered as a static parameter. However, toxicity might change as degradation processes, especially biodegradation goes by and intermediate products appear. These intermediates can even be more toxic than the original effluent was, posing higher risk to the ecosystem of the recipient water body. In our test series it was assessed how toxicity of a municipal wastewater sample changes during biodegradation taking into consideration different temperature regimes (10, 20 and 30 °C). Results proved our null hypothesis: after the high initial toxicity of the fresh effluent sample toxicity did show a further increase. Biodegradation resulted in toxicity reduction only after an approx. 2 week-period.
Anderson, D. J., Day, M. J., Russell, N. J., White, G. F. (1990) Die-away kinetic analysis of the capacity of epilithic and planktonic bacteria from clean and polluted river water to biodegrade sodium dodecyl sulphate. Appl. Environ. Microbiol. 3 , 758–763.
White G. F. , 'Die-away kinetic analysis of the capacity of epilithic and planktonic bacteria from clean and polluted river water to biodegrade sodium dodecyl sulphate ' (1990 ) 3 Appl. Environ. Microbiol. : 758 -763 .
Anon. (1999) BS EN ISO 11348.3, Part 3 — Method Using Freeze-dried Bacteria . Infonorme London Information, Ascot, UK.
Blinova, I. (2001) Use of bioassays for toxicity assessment of polluted water. Proceedings of the Symposium dedicated to the 40th Anniversary of Institute of Environmental Engineering at Tallinn Technical University, 24–26 September 2001, Tallinn , pp. 149–154.
Cairns, J. (1983) Are single species toxicity tests alone adequate for estimating environmental hazard? Hydrobiologia 100 , 47–57.
Cairns J. , 'Are single species toxicity tests alone adequate for estimating environmental hazard? ' (1983 ) 100 Hydrobiologia : 47 -57 .
Eriksson, E., Auffarth, K., Henze, H., Ledin, A. (2002) Characteristics of grey wastewater. Urban Water 4 , 85–104.
Ledin A. , 'Characteristics of grey wastewater ' (2002 ) 4 Urban Water : 85 -104 .
Eriksson, E., Auffarth, K., Eilersen, A. M., Henze, H., Ledin, A. (2003) Household chemicals and personal care products as sources for xenobiotic organic compounds in grey wastewater. Water SA 29 , 135–146.
Ledin A. , 'Household chemicals and personal care products as sources for xenobiotic organic compounds in grey wastewater ' (2003 ) 29 Water SA : 135 -146 .
Kennedy, K. J., Graham, B., Droste, L. R., Fernandes, L., Narbaitz, R. (2000) MicrotoxTM and Ceriodaphnia dubia toxicity of BKME with powdered activated carbon treatment. Water SA 2 , 205–216.
Narbaitz R. , 'MicrotoxTM and Ceriodaphnia dubia toxicity of BKME with powdered activated carbon treatment ' (2000 ) 2 Water SA : 205 -216 .
Kováts, N., Szalay, T., Kiss, I., Kárpáti, Á., Paulovits, G. (2002) Assessment of degradability in whole effluent toxicity testing using bioluminescent bacteria. Hung. J. of Ind. Chem. 30 , 271–274.
Paulovits G. , 'Assessment of degradability in whole effluent toxicity testing using bioluminescent bacteria ' (2002 ) 30 Hung. J. of Ind. Chem. : 271 -274 .
Lapertot, M., Ebrahimi, S., Oller, I., Maldonado, M. I., Gernjak, W., Malato, S., Pulgarin, C. (2008) Evaluating Microtox as a tool for biodegradability assessment of partially treated solutions of pesticides using Fe 3+ and TiO 2 solar photo-assisted processes. Ecotoxicology and Environmental Safety 69 , 546–555.
Pulgarin C. , 'Evaluating Microtox as a tool for biodegradability assessment of partially treated solutions of pesticides using Fe3+ and TiO2 solar photo-assisted processes ' (2008 ) 69 Ecotoxicology and Environmental Safety : 546 -555 .
Means, J. L., Anderson, S. J. (1981) Comparison of five different methods for measuring biodegradability in aqueous environment. Water Air Soil Poll. 16 , 301–315.
Anderson S. J. , 'Comparison of five different methods for measuring biodegradability in aqueous environment ' (1981 ) 16 Water Air Soil Poll. : 301 -315 .
Nealson, K. H., Hastings, J. W. (1979) Bacterial bioluminescence: its control and ecological significance. Microbiol. Rev. 43 , 496–518.
Hastings J. W. , 'Bacterial bioluminescence: its control and ecological significance ' (1979 ) 43 Microbiol. Rev. : 496 -518 .
Nyholm, N., Lindgaard-Jorgensen, P., Hansen, N. (1984) Biodegradation of 4-Nitrophenol in Standardized Aquatic Degradation Tests . Ecotox. Environ. Safety vol. 8, pp. 451–470.
OECD (Organisation for Economic Co-operation and Development) (1995) Detailed review paper on biodegradability testing. Environment Monograph No. 98 .
Shelton, D. R., Tiedje, J. M. (1984) General method for determining anaerobic biodegradation potential. Appl. Environ. Microbiol. 47 , 850–857.
Tiedje J. M. , 'General method for determining anaerobic biodegradation potential ' (1984 ) 47 Appl. Environ. Microbiol. : 850 -857 .
Shimp, R. J., Schwab, B. S., Larson, R. J. (1989) Adaptation to a quaternary ammonium surfactant by suspended microbial communities in a model stream. Environ. Toxicol. Chem. 8 , 723–730.
Larson R. J. , 'Adaptation to a quaternary ammonium surfactant by suspended microbial communities in a model stream ' (1989 ) 8 Environ. Toxicol. Chem. : 723 -730 .
Strevett, K., Davidova, I., Suflita, J. M. (2002) A comprehensive review of the screening methodology for anaerobic biodegradability of surfactants. Re/Views Env. Science Biotechnol. 1 , 143–167.
Suflita J. M. , 'A comprehensive review of the screening methodology for anaerobic biodegradability of surfactants ' (2002 ) 1 Re/Views Env. Science Biotechnol. : 143 -167 .
Tisler, T., Zagorc-Koncan, J., Ros, M., Cotman, M. (1999) Biodegradation and toxicity of wastewater from industry producing mineral fibres for thermal insulation. Chemosphere 38 , 1347–1352.
Cotman M. , 'Biodegradation and toxicity of wastewater from industry producing mineral fibres for thermal insulation ' (1999 ) 38 Chemosphere : 1347 -1352 .
USEPA (1993) Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms , 4th ed., EPA/600/4-90/027F. U.S. Environmental Protection Agency, National Exposure Research Laboratory, Cincinnati, OH.
'', in Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms , (1993 ) -.
USEPA (1994) Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms , 3rd ed., EPA/600/4-91/002. U.S. Environmental Protection Agency, National Exposure Research Laboratory, Cincinnati, OH.
'', in Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms , (1994 ) -.
Ward, D. M., Brock, T. D. (1976) Nutrient Limitation of Oil Biodegradation in Lakes of Varying Water Quality in Vilas County . Trans. Wisc. Acad. Sci., Vol. 64, pp. 240–249.
Wylie, G. D., Jones, J. R., Johnson, B. T. (1982) Evaluation of the river dye-away biodegradation test. JWPCF 54 , 1231–1236.
Johnson B. T. , 'Evaluation of the river dye-away biodegradation test ' (1982 ) 54 JWPCF : 1231 -1236 .
Zgajnar-Gotvajn, A., Zagorc-Koncan, J. (1998) Whole effluent and single substances approach: a tool for hazardous wastewater management. Water Sci. Technol. 37 , 219–227.
Zagorc-Koncan J. , 'Whole effluent and single substances approach: a tool for hazardous wastewater management ' (1998 ) 37 Water Sci. Technol. : 219 -227 .
Zgajnar-Gotvajn, A., Zagorc-Koncan, J. (2003) Hazard identification of pharmaceutical wastewaters using biodegradability studies. Water Sci. Technol. 47 , 197–204.
Zagorc-Koncan J. , 'Hazard identification of pharmaceutical wastewaters using biodegradability studies ' (2003 ) 47 Water Sci. Technol. : 197 -204 .