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  • 1 Istanbul University, Vezneciler Department of Molecular Biology and Genetics 34134 Istanbul Turkey
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In this study, induction of regenerable callus from mature embryos in eight Turkish barley varieties was analysed by using different plant growth regulators (PGRs). Varying concentrations (0.5–4 mg l −1 ) of 2,4-dichlorophenoxyacetic acid (2,4-D) and dicamba (3,6-dichloro- o -anisic acid) were tested for callus induction from mature embryos. Highest percent of callus induction was observed in Bornova 92 variety (98.3%) on MS medium supplemented with 4 mg l −1 dicamba. Calli were transferred to regeneration media with 0.5 mg l −1 dicamba, 0.5 mg l −1 zeatin riboside (ZR) and 2 mg l −1 thidiazuron (TDZ). Low concentrations of dicamba induced multiple shoots during callus regeneration. When the effect of precultivation with 2,4-D or dicamba on the shoot induction were evaluated, lower concentrations (< 4 mg l −1 ) of auxins have been found optimal. On the regeneration medium with 0.5 mg l −1 dicamba, shoots were able to elongate up to 20 cm and shoot numbers were between 1–23 per callus. The use of ZR led to formation of short shoot buds and somatic embryos in 2 weeks period. The effect of TDZ was different from other PGRs by inducing green solid sectors on calli surfaces (Total 51 sectors/20 callus/Akhisar variety). Five plantlets have been grown from these solid cell clumps and transferred to specific media for root formation. As a result, five varieties (Süleyman Bey, Bornova 92, Vamyk Hoca, Kaya and Akhisar) tested in our study showed the potential to produce regenerable callus by using low amounts of dicamba or TDZ. The optimization process starts from culturing embryos to plantlet formation took nearly 4 weeks.

  • Abumhadi, N., Kamenarova, K., Gecheff, K., Christov, N., Atanassov, A. (2005) Effects of chromosome reconstruction of barley genome on tissue culture response using two different regeneration systems. Plant Cell Tiss. Org. 80 , 339–342.

    Atanassov A. , 'Effects of chromosome reconstruction of barley genome on tissue culture response using two different regeneration systems ' (2005 ) 80 Plant Cell Tiss. Org. : 339 -342.

    • Search Google Scholar
  • Akula, C., Akula, A., Henry, R. (1999) Improved regeneration efficiency from mature embryos of barley cultivars. Biologia Plantarum 42 , 505–513.

    Henry R. , 'Improved regeneration efficiency from mature embryos of barley cultivars ' (1999 ) 42 Biologia Plantarum : 505 -513.

    • Search Google Scholar
  • Altinkut, A., Kazan, K., Gozukirmizi, N. (2003) Detection of AFLP polymorphism between water stress tolerant and sensitive bulks constituted based on traits associated with water stress tolerance in barley. Genet. Mol. Biol. 26 , 77–83.

    Gozukirmizi N. , 'Detection of AFLP polymorphism between water stress tolerant and sensitive bulks constituted based on traits associated with water stress tolerance in barley ' (2003 ) 26 Genet. Mol. Biol. : 77 -83.

    • Search Google Scholar
  • Ari, S., Bilgen, G., Cobanoglu, G., Gurel, F., Gozukirmizi, N. (1995) Determination of barley hybrids with molecular markers. Cereal Res. Commun. 23 , 229–234.

    Gozukirmizi N. , 'Determination of barley hybrids with molecular markers ' (1995 ) 23 Cereal Res. Commun. : 229 -234.

    • Search Google Scholar
  • Bahieldin, A., Dyer, W. E., Qu, R. (2000) Concentration effects of dicamba on shoot regeneration in wheat. Plant Breeding 119 , 437–439.

    Qu R. , 'Concentration effects of dicamba on shoot regeneration in wheat ' (2000 ) 119 Plant Breeding : 437 -439.

    • Search Google Scholar
  • Bregitzer, P. (1992) Plant regeneration and callus type in barley: effects of genotype and culture medium. Crop Sci. 32, 1108–1112.

    Bregitzer P. , 'Plant regeneration and callus type in barley: effects of genotype and culture medium ' (1992 ) 32 Crop Sci. : 1108 -1112.

    • Search Google Scholar
  • Bregitzer, P., Dahleen, L. S., Campbell, R. D. (1998) Enhancement of plant regeneration from embryogenic callus of commercial barley cultivars. Plant Cell Rep. 17 , 941–945.

    Campbell R. D. , 'Enhancement of plant regeneration from embryogenic callus of commercial barley cultivars ' (1998 ) 17 Plant Cell Rep. : 941 -945.

    • Search Google Scholar
  • Chang, Y., Zitzewitz, J., Hayes, P. M., Chen, T. H. H. (2003) High frequency plant regeneration from immature embryos of an elite barley cultivar ( Hordeum vulgare L. Cv. Morex). Plant Cell Rep. 21 , 733–738.

    Chen T. H. H. , 'High frequency plant regeneration from immature embryos of an elite barley cultivar (Hordeum vulgare L ' (2003 ) 21 Cv. Morex). Plant Cell Rep. : 733 -738.

    • Search Google Scholar
  • Cheng, T.-Y., Smith, H. H. (1975) Organogenesis from callus culture of Hordeum vulgare . Planta 123 , 307–310.

    Smith H. H. , 'Organogenesis from callus culture of Hordeum vulgare ' (1975 ) 123 Planta : 307 -310.

    • Search Google Scholar
  • Ganeshan, S., Baga, M., Harvey, B. L., Rossnagel, B. G., Scoles, G. J., Chibbar, R. N. (2003) Production of multiple shoots from thidiazuron-treated mature embryos and leaf-base/apical meristems of barley ( Hordeum vulgare ). Plant Cell Tiss. Org. 73 , 57–64.

    Chibbar R. N. , 'Production of multiple shoots from thidiazuron-treated mature embryos and leaf-base/apical meristems of barley (Hordeum vulgare) ' (2003 ) 73 Plant Cell Tiss. Org. : 57 -64.

    • Search Google Scholar
  • Gözükırmızı, N., Arı, Ş., Oraler, G., Okatan, Y., Ünsal, N.(1990) Callus induction, plant regeneration and chromosomal variations in barley. Acta Bot. Neerl. 39, 379–387.

    Ünsal N. , 'Callus induction, plant regeneration and chromosomal variations in barley ' (1990 ) 39 Acta Bot. Neerl. : 379 -387.

    • Search Google Scholar
  • Gürel, F., Gözükırmızı, N. (1999) RAPD-polymorphism between wild lines of barley ( Hordeum vulgare L.) originating from Turkey. Genetics and Breeding 29 , 19–23.

    Gözükırmızı N. , 'RAPD-polymorphism between wild lines of barley (Hordeum vulgare L.) originating from Turkey ' (1999 ) 29 Genetics and Breeding : 19 -23.

    • Search Google Scholar
  • Gürel, F., Gözükırmızı, N. (2003) Electroporation transformation of barley. In: Jackson, J. F., Linskens, H. F. (eds) Molecular Methods of Plant Analyses (Genetic transformation of plants) . Springer Berlin Heidelberg, New York, pp. 69–89.

    Gözükırmızı N. , '', in Molecular Methods of Plant Analyses (Genetic transformation of plants) , (2003 ) -.

  • Hansch, R., Koprek, T., Heydemann, H., Mendel, R. R., Schulze, J. (1996) Electroporation-mediated transient gene expression in isolated scutella of Hordeum vulgare L. Physiol. Plant 98 , 20–27.

    Schulze J. , 'Electroporation-mediated transient gene expression in isolated scutella of Hordeum vulgare L ' (1996 ) 98 Physiol. Plant : 20 -27.

    • Search Google Scholar
  • Lupotto, E. (1984) Callus induction and plant regeneration from barley mature embryos. Ann. Bot. 54 , 523–529.

    Lupotto E. , 'Callus induction and plant regeneration from barley mature embryos ' (1984 ) 54 Ann. Bot. : 523 -529.

    • Search Google Scholar
  • Lührs, R., Lörz, H. (1987) Plant regeneration in vitro from embryogenic cultures of spring- and winter-type barley ( Hordeum vulgare . L.) varieties. Theor. Appl. Genet. 75 ,16–25.

    Lörz H. , 'Plant regeneration in vitro from embryogenic cultures of spring- and winter-type barley (Hordeum vulgare. L.) varieties ' (1987 ) 75 Theor. Appl. Genet. : 16 -25.

    • Search Google Scholar
  • Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15 , 473–497.

    Skoog F. , 'A revised medium for rapid growth and bioassays with tobacco tissue cultures ' (1962 ) 15 Physiol. Plant : 473 -497.

    • Search Google Scholar
  • Murthy, B. N. S., Murch, S. J., Saxena, P. K. (1998) Thidiazuron: a potent regulator of in vitro plant morphogenesis. In Vitro Cell Dev. Biol.-Plant 34 , 267–275.

    Saxena P. K. , 'Thidiazuron: a potent regulator of in vitro plant morphogenesis ' (1998 ) 34 In Vitro Cell Dev. Biol.-Plant : 267 -275.

    • Search Google Scholar
  • Öztürk, Z. N., Talame, V., Michalowski, C. B., Gözükırmızı, N., Tuberosa, R., Bohnert, H. J. (2002) Monitoring large-scale changes in transcript abundance in drought and salt-stressed barley. Plant Mol. Biol. 48, 551–573.

    Bohnert H. J. , 'Monitoring large-scale changes in transcript abundance in drought and salt-stressed barley ' (2002 ) 48 Plant Mol. Biol. : 551 -573.

    • Search Google Scholar
  • Przetakiewicz, A., Orczyk, W., Nadolska-Orczyk, A. (2003) The effect of auxin on plant regeneration of wheat, barley and triticale. Plant Cell Tiss. Org. 73 , 245–256.

    Nadolska-Orczyk A. , 'The effect of auxin on plant regeneration of wheat, barley and triticale ' (2003 ) 73 Plant Cell Tiss. Org. : 245 -256.

    • Search Google Scholar
  • Rengel, Z. (1987) Embryogenic callus induction and plant regeneration from cultured Hordeum vulgare mature embryos. Plant Physiol. Biochem. 25 , 43–48.

    Rengel Z. , 'Embryogenic callus induction and plant regeneration from cultured Hordeum vulgare mature embryos ' (1987 ) 25 Plant Physiol. Biochem. : 43 -48.

    • Search Google Scholar
  • Weigel, R. C., Hughes, K. W. (1985) Long term regeneration by somatic embryogenesis in barley ( Hordeum vulgare L.) tissue cultures derived from apical meristem explants. Plant Cell Tiss. Org. 5 , 151–162.

    Hughes K. W. , 'Long term regeneration by somatic embryogenesis in barley (Hordeum vulgare L.) tissue cultures derived from apical meristem explants ' (1985 ) 5 Plant Cell Tiss. Org. : 151 -162.

    • Search Google Scholar

Editorial Board

      Csányi, Vilmos (Göd)
      Dudits, Dénes (Szeged)
      Falus, András (Budapest)
      Fischer, Ernő (Pécs)
      Gábriel, Róbert (Pécs)
      Gulya, Károly (Szeged)
      Gulyás, Balázs (Stockholm)
      Hajós, Ferenc (Budapest)
      Hámori, József (Budapest)
      Heszky, László (Gödöllő)
      Hideg, Éva (Szeged)
      E. Ito (Sanuki)
      Janda, Tibor (Martonvásár)
      Kavanaugh, Michael P. (Missoula)
      Kása, Péter (Szeged)
      Klein, Éva (Stockholm)
      Kovács, János (Budapest)
      Brigitte Mauch-Mani (Neuchâtel)
      Nässel, Dick R. (Stockholm)
      Nemcsók, János (Szeged)
      Péczely, Péter (Gödöllő)
      Roberts, D. F. (Newcastle-upon-Tyne)
      Sakharov, Dimitri A. (Moscow)
      Singh, Meharvan (Fort Worth)
      Sipiczky, Mátyás (Debrecen)
      Szeberényi, József (Pécs)
      Székely, György (Debrecen)
      Tari, Irma (Szeged)
      Vágvölgyi, Csaba (Szeged),
      L. Zaborszky (Newark)

 

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Acta Biologica Hungarica
Language English
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1950
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Founder Magyar Tudományos Akadémia
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ISSN 0236-5383 (Print)
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