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  • 1 University of Debrecen Department of Microbial Biotechnology and Cell Biology, Faculty of Science and Technology Debrecen Hungary
  • | 2 Eszterházy Károly College Department of Zoology, Faculty of Sciences Eger Hungary
  • | 3 University of Debrecen Department of Biochemistry, Faculty of Science and Technology Debrecen Hungary
  • | 4 University of Debrecen Department of Horticultural Sciences and Plant Biotechnology, Faculty of Agriculture Debrecen Hungary
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Coprogen production of Neurospora crassa was dependent on glucose, aspartate and iron contents as well as on initial pH of the culture media. Surplus iron and acidic pH hindered the production of coprogen as well as the transcription of the sid1 gene (NCU07117) encoding putative L-ornithine-N 5 -monooxygenase, the first enzyme in the coprogen biosynthetic pathway. High glucose (40 g/l) and aspartate (21 g/l) concentrations were beneficial for coprogen synthesis, but neither glucose nor aspartate affected the sid1 transcription. Moreover, efficient coprogen production was observed after glucose had been consumed, which suggested that N. crassa accumulated iron even in non-growing, carbon-starving cultures.

  • Balla, J., Vercelotti, G. M., Jeney, V., Yachie, A., Varga, Z., Eaton, J. W., Balla, G. (2005) Heme, heme oxygenase and ferritin. Mol. Nutr. Food Res. 49 , 1030–1043.

    Balla G. , 'Heme, heme oxygenase and ferritin ' (2005 ) 49 Mol. Nutr. Food Res. : 1030 -1043.

  • Charlang, G., Ng, B., Horowitz, N. H., Horowitz, R. M. (1981) Cellular and extracellular siderophores of Aspergillus nidulans and Penicillium chrysogenum . Mol. Cell Biol. 1 , 94–100.

    Horowitz R. M. , 'Cellular and extracellular siderophores of Aspergillus nidulans and Penicillium chrysogenum ' (1981 ) 1 Mol. Cell Biol. : 94 -100.

    • Search Google Scholar
  • Enyedy, É. A., Pócsi, I., Farkas, E. (2004) Complexation of desferricoprogen with trivalent Fe, Al, Ga, In and divalent Fe, Ni, Cu, Zn metal ions: effects of the linking chain structure on the metal binding ability of hydroxamate based siderophores. J. Inorg. Biochem. 98 , 1957–1966.

    Farkas E. , 'Complexation of desferricoprogen with trivalent Fe, Al, Ga, In and divalent Fe, Ni, Cu, Zn metal ions: effects of the linking chain structure on the metal binding ability of hydroxamate based siderophores ' (2004 ) 98 J. Inorg. Biochem. : 1957 -1966.

    • Search Google Scholar
  • Haas, H. (2003) Molecular genetics of fungal siderophore biosynthesis and uptake: the role of siderophores in iron uptake and storage. Appl. Microbiol. Biotechnol. 62 , 316–330.

    Haas H. , 'Molecular genetics of fungal siderophore biosynthesis and uptake: the role of siderophores in iron uptake and storage ' (2003 ) 62 Appl. Microbiol. Biotechnol. : 316 -330.

    • Search Google Scholar
  • Hof, C., Eisfeld, K., Welzel, K., Antelo, L., Foster, A. J., Anke, H. (2007) Ferricrocin synthesis in Magnaporthe grisea and its role in pathogenicity in rice. Mol. Plant. Pathol. 8 , 163–172.

    Anke H. , 'Ferricrocin synthesis in Magnaporthe grisea and its role in pathogenicity in rice ' (2007 ) 8 Mol. Plant. Pathol. : 163 -172.

    • Search Google Scholar
  • Hördt, W., Römheld, V., Winkelmann, G. (2000) Fusarinines and dimerum acid, mono- and dihydroxamate siderophores from Penicillium chrysogenum , improve iron utilization by strategy I and strategy II plants. BioMetals 13 , 37–47.

    Winkelmann G. , 'Fusarinines and dimerum acid, mono- and dihydroxamate siderophores from Penicillium chrysogenum, improve iron utilization by strategy I and strategy II plants ' (2000 ) 13 BioMetals : 37 -47.

    • Search Google Scholar
  • Horowitz, N. H., Charlang, G., Horn, G., Williams, N. P. (1976) Isolation and identification of the conidial germination factor of Neurospora crassa . J. Bacteriol. 127 , 135–140.

    Williams N. P. , 'Isolation and identification of the conidial germination factor of Neurospora crassa ' (1976 ) 127 J. Bacteriol. : 135 -140.

    • Search Google Scholar
  • Howard, D. H. (1999) Acquisition, transport, and storage of iron by pathogenic fungi. Clin. Microbiol. Rev. 12 , 394–404.

    Howard D. H. , 'Acquisition, transport, and storage of iron by pathogenic fungi ' (1999 ) 12 Clin. Microbiol. Rev. : 394 -404.

    • Search Google Scholar
  • Huschka, H., Naegeli, H. U., Leuenberger-Ryf, H., Keller-Schierlein, W., Winkelmann, G. (1985) Evidence for a common siderophore transport system but different siderophore receptors in Neurospora crassa . J. Bacteriol. 162 , 715–721.

    Winkelmann G. , 'Evidence for a common siderophore transport system but different siderophore receptors in Neurospora crassa ' (1985 ) 162 J. Bacteriol. : 715 -721.

    • Search Google Scholar
  • John, S. G., Ruggiero, C. E., Hersman, L. E., Tung, C. S., Neu, M. P. (2001) Siderophore mediated plutonium accumulation by Microbacterium flavescens (JG-9). Environ. Sci. Technol. 35 , 2942–2948.

    Neu M. P. , 'Siderophore mediated plutonium accumulation by Microbacterium flavescens (JG-9) ' (2001 ) 35 Environ. Sci. Technol. : 2942 -2948.

    • Search Google Scholar
  • Konetschny-Rapp, S., Huschka, H. G., Winkelmann, G., Jung, G. (1988) High-performance liquid chromatography of siderophores from fungi. Biol. Met. 1 , 9–17.

    Jung G. , 'High-performance liquid chromatography of siderophores from fungi ' (1988 ) 1 Biol. Met. : 9 -17.

    • Search Google Scholar
  • Leary, N. O., Pembroke, A., Duggan, P. F. (1992) Improving accuracy of glucose oxidase procedure for glucose determinations on discrete analyzers. Clin. Chem. 38 , 298–302.

    Duggan P. F. , 'Improving accuracy of glucose oxidase procedure for glucose determinations on discrete analyzers ' (1992 ) 38 Clin. Chem. : 298 -302.

    • Search Google Scholar
  • Lee, S. L., Chen, W. C. (1997) Optimization of medium composition for the production of glucosyltransferase by Aspergillus niger with response surface methodology. Enzyme Microbiol. Technol. 21 , 436–440.

    Chen W. C. , 'Optimization of medium composition for the production of glucosyltransferase by Aspergillus niger with response surface methodology ' (1997 ) 21 Enzyme Microbiol. Technol. : 436 -440.

    • Search Google Scholar
  • Matzanke, B. F., Bill, E., Trautwein, A. X., Winkelmann, G. (1988) Ferricrocin functions as the main intracellular iron-storage compound in mycelia of Neurospora crassa . Biol. Metals 1 , 18–25.

    Winkelmann G. , 'Ferricrocin functions as the main intracellular iron-storage compound in mycelia of Neurospora crassa ' (1988 ) 1 Biol. Metals : 18 -25.

    • Search Google Scholar
  • Pócsi, I., Jeney, V., Kertai, P., Pócsi, I., Emri, T., Gyémánt, Gy., Fésüs, L., Balla, J., Balla, Gy. (2008) Fungal siderophores function as protective agents of LDL oxidation and are promising anti-atherosclerotic metabolites in functional food. Mol. Nutr. Food Res. 52 , 1434–1447.

    Balla Gy. , 'Fungal siderophores function as protective agents of LDL oxidation and are promising anti-atherosclerotic metabolites in functional food ' (2008 ) 52 Mol. Nutr. Food Res. : 1434 -1447.

    • Search Google Scholar
  • Richardson, D. R. (2002) Iron chelators as therapeutic agents for the treatment of cancer. Crit. Rev. Oncol/Hematol. 42 , 267–281.

    Richardson D. R. , 'Iron chelators as therapeutic agents for the treatment of cancer ' (2002 ) 42 Crit. Rev. Oncol/Hematol. : 267 -281.

    • Search Google Scholar
  • Skromne, I., Sanchez, O., Aguierre, J. (1995) Starvation stress modulates the expression of the Aspergillus nidulans brlA regulatory gene. Microbiology 141 , 21–28.

    Aguierre J. , 'Starvation stress modulates the expression of the Aspergillus nidulans brlA regulatory gene ' (1995 ) 141 Microbiology : 21 -28.

    • Search Google Scholar