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  • 1 University of Szeged Department of Microbiology, Faculty of Science and Informatics Szeged Hungary
  • 2 University of Szeged Department of Food Engineering, Faculty of Engineering Szeged Hungary
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Extracellular β-glucosidase activity of 94 strains, representing 24 species of the genera Gilbertella, Mucor, Rhizomucor , and Rhizopus was evaluated in submerged culture and under solid state fermentation on wheat bran. Gilbertella persicaria G1 isolate showed the highest activity (70.9 U ml −1 ) followed by other Gilbertella (58.6–59.0 U ml −1 ) and Rhizomucor miehei isolates (29.2–42.0 U ml −1 ). Optimum temperature for enzyme production was 25 °C for Gilbertella and Mucor , and 30 °C for Rhizomucor and Rhizopus strains. Enzymes of R. miehei strains proved to be thermotolerant preserving up to 92.8% residual activity after heating to 75 °C in the presence of cellobiose substrate. Enzymes of Mucor racemosus f. chibinensis, R. miehei and Rhizopus microsporus var. oligosporus strains were activated at acidic condition (pH 4). Glucose was a strong inhibitor for each fungal β-glucosidase tested but some of them showed ethanol tolerance up to 20% (v/v). Ethanol also activated the enzyme in these strains suggesting glycosyl transferase activity.

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