Authors:
Klára Szentmihályi Hungarian Academy of Sciences Institute of Materials and Environmental Chemistry, Chemical Research Center Budapest Hungary

Search for other papers by Klára Szentmihályi in
Current site
Google Scholar
PubMed
Close
,
Anikó Gere Richter Gedeon Ltd. Budapest Hungary

Search for other papers by Anikó Gere in
Current site
Google Scholar
PubMed
Close
,
Ilona Szöllösi-Varga Szeged University Szeged Hungary

Search for other papers by Ilona Szöllösi-Varga in
Current site
Google Scholar
PubMed
Close
,
Anna Blázovics Semmelweis University Institute of Pharmacognosy Budapest Hungary

Search for other papers by Anna Blázovics in
Current site
Google Scholar
PubMed
Close
,
S. Jasztrab State Medical Center Ministry of Defence Budapest Hungary

Search for other papers by S. Jasztrab in
Current site
Google Scholar
PubMed
Close
,
Krisztina Ladó Hungarian Academy of Sciences Institute of Materials and Environmental Chemistry, Chemical Research Center Budapest Hungary

Search for other papers by Krisztina Ladó in
Current site
Google Scholar
PubMed
Close
, and
Mária Then Semmelweis University Institute of Pharmacognosy Budapest Hungary

Search for other papers by Mária Then in
Current site
Google Scholar
PubMed
Close
Restricted access

Plant drug mixtures are widely used in the adjuvant therapy of type 2 diabetes mellitus for the prevention of complications. The drug mixtures generally contribute to the efficiency of the therapy and may also reduce undesirable side effects. Two herbal extracts (lyophilized aqueous extracts of plant drug mixtures 1: Myrtilli folium, Phaseoli fructus sine seminibus and 2: Myrtilli folium, Phaseoli fructus sine seminibus, Salviae folium ) were investigated in in vitro rat models. The content of bioactive constituents (polyphenol, flavonoid and vitamin C) in plant drug mixtures and lyophilized samples was evaluated. The antioxidant activity of lyophilized extracts was determined by measuring the ferric reducing ability of the plant, Fe 2+ induced lipid peroxidation (LPO) in rat brain homogenates and NADPH (β-nicotinamide adenine dinucleotide phosphate reduced form) induced LPO in cerebral microsomes. The antioxidant activity of lyophilized extracts was compared to that of quercetin and rutin. Both teas of lyophilized extracts had significant reducing ability (2694 and 2771 μmol/l) and inhibited LPO (IC 50 28.0 and 20.6 μl in NADPH induced LPO, 17.3 and 8.7 μl in Fe 2+ induced LPO). The high concentration of polyphenol/flavonoid (12.38–13.00 and 1.45–5.22 g/100 g, respectively) and vitamin C (0.099–0.165 g/100 g) in the herbal extracts is related to their significant antioxidant properties. The tea mixtures have significant nutritional value, since the consumption of 2 or 3 cups of tea a day covers 50% of the daily requirement of vitamin C and it is also relevant polyphenol source. The high polyphenol/flavonoid content may restore the redox imbalance and contribute to the prevention of diabetic complications.

  • Agbor, G. A., Vinson, J. A., Oben, J. E., Ngogang, J. Y. N. (2006) Comparative analysis of the in vitro antioxidant activity of white and black pepper. Nutr. Res. 26 , 59–63.

    Ngogang J. Y. N. , 'Comparative analysis of the in vitro antioxidant activity of white and black pepper ' (2006 ) 26 Nutr. Res. : 59 -63 .

    • Search Google Scholar
  • Benzie, I. E. F., Strain, J. J. (1999) Ferric reducing/antioxidant power assay: Direct measure of the total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Method Enzymol. 299 , 15–27.

    Strain J. J. , 'Ferric reducing/antioxidant power assay: Direct measure of the total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration ' (1999 ) 299 Method Enzymol. : 15 -27 .

    • Search Google Scholar
  • Braughler, J. M., Duncan, L. A., Chase, R. L. (1986) The involvement of iron in lipid peroxidation. Importance of ferric to ferrous ratios in initiation. J. Biol. Chem. 261 , 10282–10289.

    Chase R. L. , 'The involvement of iron in lipid peroxidation. Importance of ferric to ferrous ratios in initiation ' (1986 ) 261 J. Biol. Chem. : 10282 -10289 .

    • Search Google Scholar
  • Czeczot, H., Tudek, B., Kusztelak, J., Szymczyk, T., Dobrowolska, B., Glinkowska, G., Malinowski, J., Strzeleczka, H. (1990) Isolation and studies of the mutagenic activity in the Ames test of flavonoids naturally occuring in medicinal herbs. Mutat. Res. 240 , 209–216.

    Strzeleczka H. , 'Isolation and studies of the mutagenic activity in the Ames test of flavonoids naturally occuring in medicinal herbs ' (1990 ) 240 Mutat. Res. : 209 -216 .

    • Search Google Scholar
  • Dietary Reference Intakes (DRI) for Vitamin A, Vitamin K, Arsenic, Boron, Chromium, Copper, Iodine, Iron, Manganese, Molybdenum, Nickel, Silicon, Vanadium, and Zinc . 2002. Food and Nutritional Board, Institute of Medicine, National Academic Press, Boston.

  • German Pharmacopoeia (Deutsches Arzneibuch, DAB) 10.ed., 1999. Deutscher Apotheker Verlag, Stuttgart.

  • Glasl, H. (1985) Photometrische normierung von Flavonoid-O und C-Glycosiden. Fresenius Z. Anal. Chem. 321 , 325–330.

    Glasl H. , 'Photometrische normierung von Flavonoid-O und C-Glycosiden ' (1985 ) 321 Fresenius Z. Anal. Chem. : 325 -330 .

    • Search Google Scholar
  • Kakkar, R., Kalra, J., Mantha, S. V., Prasad, K. (1995) Lipid-peroxidation and activity of antioxidant enzymes in diabetic rats. Mol. Cellular Biochem. 151 , 113–119.

    Prasad K. , 'Lipid-peroxidation and activity of antioxidant enzymes in diabetic rats ' (1995 ) 151 Mol. Cellular Biochem. : 113 -119 .

    • Search Google Scholar
  • Lado, C., Then, M., Varga, I., Szőke, É., Szentmihályi, K. (2004) Antioxidant property of volatile oils determined by the ferric reducing ability. Z. Naturforsch. 59 , 354–358.

    Szentmihályi K. , 'Antioxidant property of volatile oils determined by the ferric reducing ability ' (2004 ) 59 Z. Naturforsch. : 354 -358 .

    • Search Google Scholar
  • Lowry, O. H., Rosenbourgh, N. J., Farr, A. L., Randall, R. J. (1951) Protein measurement with Folin reagent. J. Biol. Chem. 193 , 265–275.

    Randall R. J. , 'Protein measurement with Folin reagent ' (1951 ) 193 J. Biol. Chem. : 265 -275 .

    • Search Google Scholar
  • Lugasi, A. (2001) Significance of antioxidant agents. Új Diéta 1 , 16–18. (in Hungarian)

    Lugasi A. , 'Significance of antioxidant agents ' (2001 ) 1 Új Diéta : 16 -18 .

  • Parthiban, A., Mohan, R., Shanmugasundaram, K. R., Shanmugasundaram, E. R. B. (1996) Amritu Bindu therapy in diabetic retinopathy: Effect on antioxidant defenses and disease process. Perfusion 9 , 280–284.

    Shanmugasundaram E. R. B. , 'Amritu Bindu therapy in diabetic retinopathy: Effect on antioxidant defenses and disease process ' (1996 ) 9 Perfusion : 280 -284 .

    • Search Google Scholar
  • Pharmacopoeia Hungarica (Ph.Hg.) VIII. ed., 2004. Medicina Press, Budapest.

  • Player, T. J., Horton, A. A. (1981) Enzymic lipid peroxidation in the microsomal fraction of rat brain. J. Neurochem. 37 , 422–426.

    Horton A. A. , 'Enzymic lipid peroxidation in the microsomal fraction of rat brain ' (1981 ) 37 J. Neurochem. : 422 -426 .

    • Search Google Scholar
  • Pusztai, P., Ruzicska, E., Blázovics, A., Tóth, M., Somogyi, A. (2002) The change of antioxidant defence system in insulin treated, nontreated and in hypertensive diabetic rats. Diabetologia 45 , 535.

    Somogyi A. , 'The change of antioxidant defence system in insulin treated, nontreated and in hypertensive diabetic rats ' (2002 ) 45 Diabetologia : 535 -.

    • Search Google Scholar
  • Rahimi, R., Nikfar, S., Larijani, S. B., Abdollah, M. (2005) A review on the role of antioxidants in the managements of diabetes and its complications. Biomed. Pharmacother 59 , 365–373.

    Abdollah M. , 'A review on the role of antioxidants in the managements of diabetes and its complications ' (2005 ) 59 Biomed. Pharmacother : 365 -373 .

    • Search Google Scholar
  • Rahman, A. U., Zaman, K. (1989) Medicinal plants with hypoglycemic activity. J. Ethnopharm. 26 , 1–55.

    Zaman K. , 'Medicinal plants with hypoglycemic activity ' (1989 ) 26 J. Ethnopharm. : 1 -55 .

    • Search Google Scholar
  • Recommended Dietary Allowances (RDA) 10 th ed., 1989. National Academy Press, NY, Washington D. C.

  • Sabu, M. C., Kuttan, R. (2003) Antioxidant activity of Indian herbal drugs in rats with aloxan-induced diabetes. Pharm. Biol. 41 , 500–505.

    Kuttan R. , 'Antioxidant activity of Indian herbal drugs in rats with aloxan-induced diabetes ' (2003 ) 41 Pharm. Biol. : 500 -505 .

    • Search Google Scholar
  • Smidt, L. J., Cremin, F. M., Grivetti, L. E., Clifford, A. J. (1990) Influence of folate status and polyphenol intake on thiamin status of Irish women. Am. J. Clin. Nutr. 52 , 1077–1092.

    Clifford A. J. , 'Influence of folate status and polyphenol intake on thiamin status of Irish women ' (1990 ) 52 Am. J. Clin. Nutr. : 1077 -1092 .

    • Search Google Scholar
  • Stefanovits-Bányai, É., Blázovics, A., Koczka, N. (2002) Antioxidant effect of Ginko biloba L. originated from Hungary. Free Rad. Biol. Med. 33 , 557.

    Koczka N. , 'Antioxidant effect of Ginko biloba L. originated from Hungary ' (2002 ) 33 Free Rad. Biol. Med. : 557 -.

    • Search Google Scholar
  • Szentmihályi, K., Hajdú, M., Fodor, J., Kótai, L., Blázovics, A., Then, M. (2006) In vitro study of elements in herbal remedies. Biol. Trace Elem. Res. 114 , 143–150.

    Then M. , 'In vitro study of elements in herbal remedies ' (2006 ) 114 Biol. Trace Elem. Res. : 143 -150 .

    • Search Google Scholar
  • Then, M., Szöllösy, R., Vásárhelyi-Perédi, K., Szentmihályi, K. (2004) Polyphenol-, mineral element content and total antioxidant power of sage ( Salvia officinalis L.) extracts. Acta Hort. 629 , 123–129.

    Szentmihályi K. , 'Polyphenol-, mineral element content and total antioxidant power of sage (Salvia officinalis L.) extracts ' (2004 ) 629 Acta Hort. : 123 -129 .

    • Search Google Scholar
  • Then, M., Szentmihályi, K., Gere, A., Jasztrab, S., Szőke, É. (2005) Antioxidant properties of Myrtilli folium, Phaseoli fructus sine seminibus and drug mixture extracts. Acta Aliment. 34 , 169–176.

    Szőke , 'Antioxidant properties of Myrtilli folium, Phaseoli fructus sine seminibus and drug mixture extracts ' (2005 ) 34 Acta Aliment. : 169 -176 .

    • Search Google Scholar
  • Varma, S. D., Devamanoharan, P. S., Ali, A. H. (1997) Formation of advanced glycation end (AGE) products in diabetes: Prevention by pyruvate and alpha-keto-glutarate Mol. Cell Biochem. 171 , 23–28.

    Ali A. H. , 'Formation of advanced glycation end (AGE) products in diabetes: Prevention by pyruvate and alpha-keto-glutarate ' (1997 ) 171 Mol. Cell Biochem. : 23 -28 .

    • Search Google Scholar
  • Collapse
  • Expand

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

Acta Biologica Hungarica
P.O. Box 35
H-8237 Tihany, Hungary
Phone: (36 87) 448 244 ext. 103
Fax: (36 87) 448 006
E-mail: elekes@tres.blki.hu

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture
  • Biology and Environmental Sciences
  • Elsevier BIOBASE
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Acta Biologica Hungarica
Language English
Size  
Year of
Foundation
1950
Publication
Programme
changed title
Volumes
per Year
 
Issues
per Year
 
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)