Contributions of preventive and antioxidant (energy dissipating and singlet oxygen neutralizing) processes to tolerating high light stress (photoinhibition) were examined in green-house grown tobacco (Nicotiana tabacum) plants acclimated to high or low light conditions and also in sun and shade leaves collected from a natural grown linden tree (Tilia platyphyllos). Tobacco leaves survived a short (1 h) exposure to photoinhibition by activating non-regulated non-photochemical quenching [Y(NO)] rather than relying on photo-protective, regulated non-photochemical quenching [Y(NPQ)]. Low light acclimated leaves had lower singlet oxygen scavenging ability and activated Y(NO) to a larger extent than high light acclimated ones. Low light grown leaves also suffered singlet oxygen mediated photo-damage, while no singlet oxygen was detected in high light acclimated leaves during photoinhibition. Natural grown linden leaves, however, coped with prolonged daily exposures to high light mainly by activating regulated non-photochemical quenching Y(NPQ), although they also featured very efficient singlet oxygen neutralizing. Our results suggest that high light tolerance is achieved by preventing photoinhibition of photosystem II via efficient photo-protective energy dissipation rather than relying on quenching of stress-induced pro-oxidative agents.
Apel, K., Hirt, H. (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu. Rev. Plant Biol. 55, 373–399.
Hirt H. , 'Reactive oxygen species: metabolism, oxidative stress, and signal transduction ' (2004 ) 55 Annu. Rev. Plant Biol. : 373 -399 .
Aro, E.-M., Virgin, I., Andersson, B. (1993) Photoinhibition of Photosystem II. Inactivation, protein damage and turnover. Biochim. Biophys. Acta 1143, 113–134.
Andersson B. , 'Photoinhibition of Photosystem II. Inactivation, protein damage and turnover ' (1993 ) 1143 Biochim. Biophys. Acta : 113 -134 .
Asada, K. (1994) Production and action of active oxygen species in photosynthetic tissues. In: Foyer, Ch. F., Mullineaux, P. M. (eds) Causes of Photooxidative Stress and Amelioration of Defence Systems in Plants. CRC Press, Boca Raton, FL, pp. 77–104.
Asada K. , '', in Causes of Photooxidative Stress and Amelioration of Defence Systems in Plants , (1994 ) -.
Baker, N. R. (2008) Chlorophyll fluorescence: A probe of photosynthesis in vivo. Annu. Rev. Plant Biol. 59, 89–113.
Baker N. R. , 'Chlorophyll fluorescence: A probe of photosynthesis in vivo ' (2008 ) 59 Annu. Rev. Plant Biol. : 89 -113 .
Barber, J. Andersson, B. (1992) Too much for a good thing: light can be bad for photosynthesis. Trends Biochem. Sci. 17, 61–66.
Andersson B. , 'Too much for a good thing: light can be bad for photosynthesis ' (1992 ) 17 Trends Biochem. Sci. : 61 -66 .
Demmig-Adams, B., Adams, W. W. II (1992) Photoprotection and other responses of plants to high light stress. Annu. Rev. Plant Physiol. Plant Mol. Biol. 43, 599–626.
Adams W. W. I. , 'Photoprotection and other responses of plants to high light stress ' (1992 ) 43 Annu. Rev. Plant Physiol. Plant Mol. Biol. : 599 -626 .
Genty, B., Briantais, J.-M., Baker, N. R. (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim. Biophys. Acta 990, 87–92.
Baker N. R. , 'The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence ' (1989 ) 990 Biochim. Biophys. Acta : 87 -92 .
Genty, B., Harbinson, J., Cailly, A. L., Rizza, F. (1996) Fate of excitation at PS II in leaves: the nonphotochemical side. Presented at The Third BBSRC Robert Hill Symposium on Photosynthesis, March 31 to April 3, 1996, University of Sheffield, Department of Molecular Biology and Biotechnology, Western Bank, Sheffield, UK, Abstract no. P28.
Hendrickson, L., Furbank, R. T., Chow, W. S. (2004) A simple alternative approach to assessing the fate of absorbed light energy using chlorophyll fluorescence. Photosynth. Res. 82, 73–81.
Chow W. S. , 'A simple alternative approach to assessing the fate of absorbed light energy using chlorophyll fluorescence ' (2004 ) 82 Photosynth. Res. : 73 -81 .
Hideg, É. (2008) A comparative study of fluorescent singlet oxygen probes in plant leaves. Centr. Eur. J. Biol. 3, 273–284
Hideg , 'A comparative study of fluorescent singlet oxygen probes in plant leaves ' (2008 ) 3 Centr. Eur. J. Biol. : 273 -284 .
Hideg, É., Schreiber, U. (2007) Parallel assessment of ROS formation and photosynthesis in leaves by fluorescence imaging. Photosynth. Res. 92, 103–108.
Schreiber U. , 'Parallel assessment of ROS formation and photosynthesis in leaves by fluorescence imaging ' (2007 ) 92 Photosynth. Res. : 103 -108 .
Hideg, É., Kálai, T., Hideg, K., Vass, I. (1998) Photoinhibition of photosynthesis in vivo results in singlet oxygen production. Detection via nitroxide-induced fluorescence quenching in broad bean leaves. Biochemistry 37, 11405–11411.
Vass I. , 'Photoinhibition of photosynthesis in vivo results in singlet oxygen production. Detection via nitroxide-induced fluorescence quenching in broad bean leaves ' (1998 ) 37 Biochemistry : 11405 -11411 .
Hurry, V., Anderson, J. M., Chow, W. S., Osmond, C. B. (1997) Accumulation of zeaxanthin in abscisic acid-deficient mutants of Arabidopsis does not affect chlorophyll fluorescence quenching or sensitivity to photoinhibition in vivo. Plant Physiol. 113, 639–648.
Osmond C. B. , 'Accumulation of zeaxanthin in abscisic acid-deficient mutants of Arabidopsis does not affect chlorophyll fluorescence quenching or sensitivity to photoinhibition in vivo ' (1997 ) 113 Plant Physiol. : 639 -648 .
Ivanova, D., Gerova, D., Chervenkov, T., Yankova, T. (2005) Polyphenols and antioxidant capacity of Bulgarian medicinal plants. J. Ethnopharmacol. 96, 145–150.
Yankova T. , 'Polyphenols and antioxidant capacity of Bulgarian medicinal plants ' (2005 ) 96 J. Ethnopharmacol. : 145 -150 .
Kálai, T., Hideg, É., Vass, I., Hideg, K. (1998) Double (fluorescent and spin) sensors for detection of reactive oxygen species in the thylakoid membrane. Free Rad. Biol. Med. 24, 649–652.
Hideg K. , 'Double (fluorescent and spin) sensors for detection of reactive oxygen species in the thylakoid membrane ' (1998 ) 24 Free Rad. Biol. Med. : 649 -652 .
Klughammer, Ch., Schreiber, U. (2008) Complementary PS II quantum yields calculated from simple fluorescence parameters measured by PAM fluorometry and the Saturation Pulse method. PAM Application Notes 1, 27–35.
Schreiber U. , 'Complementary PS II quantum yields calculated from simple fluorescence parameters measured by PAM fluorometry and the Saturation Pulse method ' (2008 ) 1 PAM Application Notes : 27 -35 .
Kraljic, I., El Moshni, S. (1978) A new method for the detection of singlet oxygen in aqueous solutions. Photochem. Photobiol. 28, 577–581.
Moshni S. , 'A new method for the detection of singlet oxygen in aqueous solutions ' (1978 ) 28 Photochem. Photobiol. : 577 -581 .
Kramer, D. M., Johnson, G., Kiirats, O., Edwards, G. E. (2004) New fluorescence parameters for the determination of QA redox state and excitation energy fluxes. Photosynth. Res. 79, 209–218.
Edwards G. E. , 'New fluorescence parameters for the determination of QA redox state and excitation energy fluxes ' (2004 ) 79 Photosynth. Res. : 209 -218 .
Krause, G. H. (1994) The role of oxygen in photoinhibition of photosynthesis. In: Foyer, Ch. F., Mullineaux, P. M. (eds) Causes of Photooxidative Stress and Amelioration of Defence Systems in Plants. CRC Press, Boca Raton, FL, pp. 43–76.
Krause G. H. , '', in Causes of Photooxidative Stress and Amelioration of Defence Systems in Plants , (1994 ) -.
Krause, G. H., Virgo, A., Winter, K. (1995) High susceptibility to photoinhibition of young leaves of tropical forest trees. Planta 197, 583–591.
Winter K. , 'High susceptibility to photoinhibition of young leaves of tropical forest trees ' (1995 ) 197 Planta : 583 -591 .
Ögren, E. (1988) Photoinhibition of photosynthesis in willow leaves under field conditions. Planta 175, 229–236.
Ögren E. , 'Photoinhibition of photosynthesis in willow leaves under field conditions ' (1988 ) 175 Planta : 229 -236 .
Powles, S. B. (1984) Photoinhibition of photosynthesis induced by visible light. Annu. Rev. Plant Physiol. 35, 15–44.
Powles S. B. , 'Photoinhibition of photosynthesis induced by visible light ' (1984 ) 35 Annu. Rev. Plant Physiol. : 15 -44 .
Prasil, O., Adir, N., Ohad, I. (1992) Dynamics of photosystem II: mechanism of photoinhibition and recovery process. In Barber, J. (ed.) Topics in Photosynthesis, The Photosystems: Structure, Function and Molecular Biology. Vol. 11. Elsevier, Amsterdam, pp. 220–250.
Ohad I. , '', in Topics in Photosynthesis, The Photosystems: Structure, Function and Molecular Biology. Vol. 11 , (1992 ) -.
Schreiber, U. Schliwa, U. Bilger, W. (1986) Continuous recording of photochemical and nonphotochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. Photosynth. Res. 10, 51–62.
Bilger W. , 'Continuous recording of photochemical and nonphotochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer ' (1986 ) 10 Photosynth. Res. : 51 -62 .
Schreiber, U., Hormann, H., Neubauer, C., Klughammer, Ch. (1995) Assessment of photosystem II photochemical quantum yield by chlorophyll fluorescence quenching analysis. Aust. J. Plant Physiol. 22, 209–220.
Klughammer Ch. , 'Assessment of photosystem II photochemical quantum yield by chlorophyll fluorescence quenching analysis ' (1995 ) 22 Aust. J. Plant Physiol. : 209 -220 .
Sharma, J., Panico, M., Barber, J., Morris, H. R. (1997) Characterization of the low molecular weight photosystem II reaction center subunits and their light-induced modifications by mass spectrometry. J. Biol. Chem. 272, 3935–3943.
Morris H. R. , 'Characterization of the low molecular weight photosystem II reaction center subunits and their light-induced modifications by mass spectrometry ' (1997 ) 272 J. Biol. Chem. : 3935 -3943 .
Trtilek, M., Krame, D. M., Koblizek, M., Nedbal, L. (1997) Dual-modulation LED kinetic fluorometer. J. Lumin. 72–74, 597–599.
Nedbal L. , 'Dual-modulation LED kinetic fluorometer ' (1997 ) 72–74 J. Lumin. : 597 -599 .
Vass, I., Styring, S. (1992) Spectroscopic characterization of triplet forming states in photosystem II. Biochemistry 31, 5957–5963.
Styring S. , 'Spectroscopic characterization of triplet forming states in photosystem II ' (1992 ) 31 Biochemistry : 5957 -5963 .
Yildirim, A., Mavi, A., Oktay, M., Kara, A. A., Algur, O. F., Bilaloglu, V. (2000) Comparison of antioxidant and antimicrobial activities of tilia (Tilia argentea Desf. ex DC.), sage (Salvia triloba L.), and black tea (Camellia sinensis) extracts. J. Agric. Food. Chem. 48, 5030–5034.
Bilaloglu V. , 'Comparison of antioxidant and antimicrobial activities of tilia (Tilia argentea Desf. ex DC.), sage (Salvia triloba L.), and black tea (Camellia sinensis) extracts ' (2000 ) 48 J. Agric. Food. Chem. : 5030 -5034 .