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A. Mahesh

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R. Jeyachandran St. Joseph’s College Department of Plant Biology and Plant Biotechnology Tiruchirappalli 620002 Tamil Nadu India

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L. Cindrella National Institute of Technology Department of Chemistry Tiruchirappalli 620015 Tamil Nadu India

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D. Thangadurai Karnatak University Department of Botany Dharwad 580003 Karnataka India

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V. Veerapur SET College of Pharmacy Department of Pharmaceutical Chemistry Dharwad 580002 Karnataka India

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D. Muralidhara Rao Sri Krishnadevaraya University Department of Biotechnology Anantapur 515003 Andhra Pradesh India

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The hepatocurative potential of ethanolic extract (ETO) and sesquiterpene lactones enriched fraction (SL) of Taraxacum officinale roots was evaluated against carbon tetrachloride (CCl 4 ) induced hepatotoxicity in mice. The diagnostic markers such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and total bilirubin contents were significantly elevated, whereas significant reduction in the level of reduced glutathione (GSH) and enhanced hepatic lipid peroxidation, liver weight and liver protein were observed in CCl 4 induced hepatotoxicity in mice. Post-treatment with ETO and SL significantly protected the hepatotoxicity as evident from the lower levels of hepatic enzyme markers, such as serum transaminase (ALT, AST), ALP and total bilirubin. Further, significant reduction in the liver weight and liver protein in drug-treated hepatotoxic mice and also reduced oxidative stress by increasing reduced glutathione content and decreasing lipid peroxidation level has been noticed. The histopathological evaluation of the liver also revealed that ETO and SL reduced the incidence of liver lesions induced by CCl 4 . The results indicate that sesquiterpene lactones have a protective effect against acute hepatotoxicity induced by the administration of CCl 4 in mice. Furthermore, observed activity of SL may be due to the synergistic action of two sesquiterpene lactones identified from enriched ethyl acetate fraction by HPLC method.

  • Ahmed, B., Alam, T., Varshney, M., Khan, A. S. (2002) Hepatoprotective activity of two plants belonging to the Apiaceae and the Euphorbiaceae family. J. Ethnopharmacol. 79 , 313–316.

    Khan A. S. , 'Hepatoprotective activity of two plants belonging to the Apiaceae and the Euphorbiaceae family ' (2002 ) 79 J. Ethnopharmacol. : 313 -316 .

    • Search Google Scholar
  • Banskota, A. H., Tezuka, Y., Adnyana, I. K., Xiong, Q., Hase, K., Tran, K. Q., Tanaka, K., Saiki, J., Kadota, S. (2000) Hepatoprotective effect of Combretum quadrangulare and its constituents. Biol. Pharm. Bull. 23 , 456–460.

    Kadota S. , 'Hepatoprotective effect of Combretum quadrangulare and its constituents ' (2000 ) 23 Biol. Pharm. Bull. : 456 -460 .

    • Search Google Scholar
  • Basu, S. (2003) Carbon tetrachloride-induced lipid peroxidation: Eicosanoid formation and their regulation by antioxidant nutrients. Toxicology189 , 113–127.

    Basu S. , 'Carbon tetrachloride-induced lipid peroxidation: Eicosanoid formation and their regulation by antioxidant nutrients ' (2003 ) 189 Toxicology : 113 -127 .

    • Search Google Scholar
  • Bhandarkar, M. R., Khan, A. (2004) Antihepatotoxic effect of Nymphaea stellata willd. against carbon tetrachloride induced hepatic damage in albino rats. J. Ethnopharmacol. 91 , 61–64.

    Khan A. , 'Antihepatotoxic effect of Nymphaea stellata willd. against carbon tetrachloride induced hepatic damage in albino rats ' (2004 ) 91 J. Ethnopharmacol. : 61 -64 .

    • Search Google Scholar
  • Bown, D. (1995) Encyclopedia of Herbs and Their Uses . Dorling Kindersley, London, pp. 20–31.

    Bown D. , '', in Encyclopedia of Herbs and Their Uses , (1995 ) -.

  • Chiej, R. (1984) Encyclopedia of Medicinal Plants . Mac Donald, USA.

    Chiej R. , '', in Encyclopedia of Medicinal Plants , (1984 ) -.

  • De Feudis, F. V., Papadopoulos, V., Drieu, K. (2003) Ginkgo biloba extracts and cancer: A research area in its infancy. Fundam. Clin. Pharmacol. 17 , 405–417.

    Drieu K. , 'Ginkgo biloba extracts and cancer: A research area in its infancy ' (2003 ) 17 Fundam. Clin. Pharmacol. : 405 -417 .

    • Search Google Scholar
  • Eisisi, A. E., Earnest, D. L., Sipes, I. G. (1993) Vitamin A potentiation of carbon tetrachloride hepatoxicity: Role of liver macrophages and active oxygen species. Toxicol. Appl. Pharmacol. 119 , 295–301.

    Sipes I. G. , 'Vitamin A potentiation of carbon tetrachloride hepatoxicity: Role of liver macrophages and active oxygen species ' (1993 ) 119 Toxicol. Appl. Pharmacol. : 295 -301 .

    • Search Google Scholar
  • Ellman, G. L. (1959) Tissue sulphydryl group. Arch. Biochem. Biophys. 82 , 70–77.

    Ellman G. L. , 'Tissue sulphydryl group ' (1959 ) 82 Arch. Biochem. Biophys. : 70 -77 .

    • Search Google Scholar
  • Esterbauer, H., Cheeseman, K. H. (1990) Determination of aldehydic lipid peroxidation products: Malonaldehyde and 4-hydroxynonenal. Meth. Enzymol. 186 , 407–421.

    Cheeseman K. H. , 'Determination of aldehydic lipid peroxidation products: Malonaldehyde and 4-hydroxynonenal ' (1990 ) 186 Meth. Enzymol. : 407 -421 .

    • Search Google Scholar
  • Foster, S., Duke, J. A. (1990) A Field Guide to Medicinal Plants of Eastern and Central North America . Houghton Mifflin Co., Boston.

    Duke J. A. , '', in A Field Guide to Medicinal Plants of Eastern and Central North America , (1990 ) -.

  • Goeptar, A. R., Scheerens, H., Vermeulen, N. P. (1995) Oxygen and xenobiotic reductase activities of cytochrome P450. Crit. Rev. Toxicol. 25 , 25–65.

    Vermeulen N. P. , 'Oxygen and xenobiotic reductase activities of cytochrome P450 ' (1995 ) 25 Crit. Rev. Toxicol. : 25 -65 .

    • Search Google Scholar
  • Gueeri, H. (1995) Influence on prolonged ethanol intake on the level and turnover of alcohol and aldehyde dehydrogenase and glutathione. Adv. Exp. Med. Biol. 23 , 133–134.

    Gueeri H. , 'Influence on prolonged ethanol intake on the level and turnover of alcohol and aldehyde dehydrogenase and glutathione ' (1995 ) 23 Adv. Exp. Med. Biol. : 133 -134 .

    • Search Google Scholar
  • Harper, H. A. (1961) The Functions and Tests of the Liver, Review of Physiological Chemistry . Lange Medical Publishers, Los Atlos, CA, pp. 271–283.

    Harper H. A. , '', in The Functions and Tests of the Liver, Review of Physiological Chemistry , (1961 ) -.

  • Jeon, T. I., Hwang, S. G., Lim, B. O. (2003) Extract of Phellinus linteus grown on germinated brown rice suppress liver damage induced by carbon tetrachloride in rats. Biotech. Lett. 25 , 2093–2096.

    Lim B. O. , 'Extract of Phellinus linteus grown on germinated brown rice suppress liver damage induced by carbon tetrachloride in rats ' (2003 ) 25 Biotech. Lett. : 2093 -2096 .

    • Search Google Scholar
  • Jin Lee, K., Woo, E. R., Choi, C. Y. (2004) Protective effect of acteoside on carbon tetrachloride-induced hepatotoxicity. Life Sci. 74 , 1051–1064.

    Choi C. Y. , 'Protective effect of acteoside on carbon tetrachloride-induced hepatotoxicity ' (2004 ) 74 Life Sci. : 1051 -1064 .

    • Search Google Scholar
  • Kadiiska, M. B., Gladen, B. C., Baird, D. D., Dikalov, A. E., Sohal, R. S., Hatch, G. B., Jones, D. P., Mason, R. P., Barret, J. C. (2000) Biomarkers of oxidative stress study: Are plasma antioxidants markers of CCl 4 poisoning? Free Rad. Biol. Med. 28 , 838–845.

    Barret J. C. , 'Biomarkers of oxidative stress study: Are plasma antioxidants markers of CCl4 poisoning? ' (2000 ) 28 Free Rad. Biol. Med. : 838 -845 .

    • Search Google Scholar
  • Kaur, G., Jabbar, Z., Athar, M., Alam, M. S. (2006) Punica granatum (pomegranate) flower extract possesses potent antioxidant activity and abrogates Fe-NTA induced hepatotoxicity in mice. Food and Chemical Toxicology44 , 984–993.

    Alam M. S. , 'Punica granatum (pomegranate) flower extract possesses potent antioxidant activity and abrogates Fe-NTA induced hepatotoxicity in mice ' (2006 ) 44 Food and Chemical Toxicology : 984 -993 .

    • Search Google Scholar
  • Kisiel, W., Michalska, K. (2006) Matricarin-type guaianolides from Taraxacum bessarabicum and their chemotaxonomic significance. Biochemical Systematics and Ecology34 , 356–359.

    Michalska K. , 'Matricarin-type guaianolides from Taraxacum bessarabicum and their chemotaxonomic significance ' (2006 ) 34 Biochemical Systematics and Ecology : 356 -359 .

    • Search Google Scholar
  • Ko, K. M., Ip, S. P., Poon, M. K. T., Wu, S. S., Che, C. T., Ng, K. H. (1995) Effect of lignan enriched Fructus schisandrae extract on hepatic glutathione status in rats: Protection against carbon tetrachloride toxicity. Planta Med. 61 , 134–137.

    Ng K. H. , 'Effect of lignan enriched Fructus schisandrae extract on hepatic glutathione status in rats: Protection against carbon tetrachloride toxicity ' (1995 ) 61 Planta Med. : 134 -137 .

    • Search Google Scholar
  • Launert, E. (1981) Edible and Medicinal Plants . Hamlyn, UK.

    Launert E. , '', in Edible and Medicinal Plants , (1981 ) -.

  • Liu, J. Y., Chen, C. C., Wang, W. H., Hsu, J. D., Yang, M. Y., Wang, C. J. (2006) The protective effects of Hibiscus sabdariffa extract on CCl4-induced liver fibrosis in rats. Food and Chemical Toxicology44 , 336–343.

    Wang C. J. , 'The protective effects of Hibiscus sabdariffa extract on CCl4-induced liver fibrosis in rats ' (2006 ) 44 Food and Chemical Toxicology : 336 -343 .

    • Search Google Scholar
  • Li-Weber, M., Giaisi, M., Baumann, S., Treiber, M. K., Krammer, P. H. (2002) The anti-inflammatory sesquiterpene lactone, parthenolide suppresses CD95-mediated activation-induced-cell-death in T-cells. Cell Death and Differentiation9 , 1256–1265.

    Krammer P. H. , 'The anti-inflammatory sesquiterpene lactone, parthenolide suppresses CD95-mediated activation-induced-cell-death in T-cells ' (2002 ) 9 Cell Death and Differentiation : 1256 -1265 .

    • Search Google Scholar
  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J. (1951) Protein measurement with folin phenol reagent. J. Biol. Chem. 193 , 265–275.

    Randall R. J. , 'Protein measurement with folin phenol reagent ' (1951 ) 193 J. Biol. Chem. : 265 -275 .

    • Search Google Scholar
  • Mahesh, A., Jeyachandran, R., Cindrella, L., Thangadurai, D., Velayutham, R., Pinheiro de Carvalho, M. A. A. (2008) In: Thangadurai, D., Tang, W., Ramachandran, A., Moraes, I. O., Pinheiro de Carvalho M. A. A. (eds) Biotechnology for Food, Environment and Agriculture . Agrobios (India), Jodhpur, pp. 235–251.

  • Mansour, M. A. (2000) Protective effects of thymoquinone and desferrioxamine against hepatotoxicity of carbon tetrachloride in mice. Life Sci. 66 , 2583–2591.

    Mansour M. A. , 'Protective effects of thymoquinone and desferrioxamine against hepatotoxicity of carbon tetrachloride in mice ' (2000 ) 66 Life Sci. : 2583 -2591 .

    • Search Google Scholar
  • Meijer, D. K. F., Smit, J. W., Muller, M. (1997) Hepatobiliary elimination of cationic drugs: The role of P-glycoproteins and other ATP dependent transporters. Adv. Drug Deliv. Rev. 25 , 159–200.

    Muller M. , 'Hepatobiliary elimination of cationic drugs: The role of P-glycoproteins and other ATP dependent transporters ' (1997 ) 25 Adv. Drug Deliv. Rev. : 159 -200 .

    • Search Google Scholar
  • Molander, D. W., Wroblewsk, F., La Due, J. S. (1955) Transaminase compared with cholinesterase and alkaline phosphatase as an index of hepatocellular integrity. Clin. Res. Proc. 3 , 20–24.

    Due J. S. , 'Transaminase compared with cholinesterase and alkaline phosphatase as an index of hepatocellular integrity ' (1955 ) 3 Clin. Res. Proc. : 20 -24 .

    • Search Google Scholar
  • Myagmar, B. E., Shinno, E., Ichiba, T., Aniya, Y. (2004) Antioxidant activity of medicinal herb Rhodococcum vitis-idaea on galactosamine induced liver injury in rats. Phytomedicine11 , 416–423.

    Aniya Y. , 'Antioxidant activity of medicinal herb Rhodococcum vitis-idaea on galactosamine induced liver injury in rats ' (2004 ) 11 Phytomedicine : 416 -423 .

    • Search Google Scholar
  • Ohkawa, H., Ohishi, N., Yagi, K. (1979) Assay of lipid peroxides in animal tissue by thiobarbituric acid reaction. Anal Biochem. 95 , 351–358.

    Yagi K. , 'Assay of lipid peroxides in animal tissue by thiobarbituric acid reaction ' (1979 ) 95 Anal Biochem. : 351 -358 .

    • Search Google Scholar
  • Ohta, Y., Nishida, K., Sasaki, E., Kongo, M., Ishiguro, I. (1997) Attenuation of disrupted hepatic active oxygen metabolism with the recovery of acute liver injury in rats intoxicated with carbon tetrachloride. Res. Commun. Mol. Pathol. Pharmacol. 95 , 191–207.

    Ishiguro I. , 'Attenuation of disrupted hepatic active oxygen metabolism with the recovery of acute liver injury in rats intoxicated with carbon tetrachloride ' (1997 ) 95 Res. Commun. Mol. Pathol. Pharmacol. : 191 -207 .

    • Search Google Scholar
  • Ozturk, F., Ucar, M., Ozturk, I. C., Vardi, N., Batcioglu, K. (2003) Carbon tetrachloride induced nephrotoxicity and protective effect of betaine in Sprague-Dawley rats. Urology62 , 353–356.

    Batcioglu K. , 'Carbon tetrachloride induced nephrotoxicity and protective effect of betaine in Sprague-Dawley rats ' (2003 ) 62 Urology : 353 -356 .

    • Search Google Scholar
  • Paradise, V., Kollinger, M., Fabre, M., Holstege, A., Poynard, T., Beddosa, P. (1997) In situ detection of lipid peroxidation by products in chronic liver diseases. Hepatology26 , 135–142.

    Beddosa P. , 'In situ detection of lipid peroxidation by products in chronic liver diseases ' (1997 ) 26 Hepatology : 135 -142 .

    • Search Google Scholar
  • Plaa, G. L., De Lamirande, E., Lewittes, M., Yousef, I. M. (1982) Liver cell plasma membrane lipids in manganese-bilirubin induced intrahepatic cholestasis. Biochem. Pharmacol. 31 , 3698–3701.

    Yousef I. M. , 'Liver cell plasma membrane lipids in manganese-bilirubin induced intrahepatic cholestasis ' (1982 ) 31 Biochem. Pharmacol. : 3698 -3701 .

    • Search Google Scholar
  • Recknagel, R. O., Glende Jr., E. A., Dolak, J. A., Waller, R. L. (1989) Mechanisms of carbon tetrachloride toxicity. Pharmacology and Therapeutics43 , 139–154.

    Waller R. L. , 'Mechanisms of carbon tetrachloride toxicity ' (1989 ) 43 Pharmacology and Therapeutics : 139 -154 .

    • Search Google Scholar
  • Recknagel, R. O., Glende Jr, E. A., Britton, R. S. (1991) Free radical damage and lipid peroxidation. In: Meeks, R. G. (eds) Hepatotoxicology . CRC Press, Florida, pp. 401–436.

    Britton R. S. , '', in Hepatotoxicology , (1991 ) -.

  • Sheweita, S. A., El-Gabar, M. A., Bastawy, M. (2001) Carbon tetrachloride changes the activity of cytochrome P450 system in the liver of male rats: Role of antioxidants. Toxicology165 , 217–224.

    Bastawy M. , 'Carbon tetrachloride changes the activity of cytochrome P450 system in the liver of male rats: Role of antioxidants ' (2001 ) 165 Toxicology : 217 -224 .

    • Search Google Scholar
  • Simmons, J. E., Yang, R. S., Berman, E. (1995) Evaluation of the nephrotoxicity of complex mixtures containing organics and metals: Advantages and disadvantages of the use of real-world complex mixtures. Environ. Health Perspect. 103 , 67–71.

    Berman E. , 'Evaluation of the nephrotoxicity of complex mixtures containing organics and metals: Advantages and disadvantages of the use of real-world complex mixtures ' (1995 ) 103 Environ. Health Perspect. : 67 -71 .

    • Search Google Scholar
  • Siyuan, Z., Yen-Kim, W., Choon-Nam, O., Han-Ming, S. (2005) Anti-cancer potential of sesquiterpene lactones: Bioactivity and molecular mechanisms. Current Medicinal Chemistry5 , 239–249.

    Han-Ming S. , 'Anti-cancer potential of sesquiterpene lactones: Bioactivity and molecular mechanisms ' (2005 ) 5 Current Medicinal Chemistry : 239 -249 .

    • Search Google Scholar
  • Takeoka, G. R., Dao, L. T. (2003) Antioxidant constituent of almond ( Prunus dulcis (Mill.) D. A. Webb.) hulls. J. Agric. Food Chem. 51 , 496–501.

    Dao L. T. , 'Antioxidant constituent of almond (Prunus dulcis (Mill.) D. A. Webb.) hulls ' (2003 ) 51 J. Agric. Food Chem. : 496 -501 .

    • Search Google Scholar
  • Tang, X., Gao, J., Wang, Y., Fan, Y. M., Xu, L. Z., Zhao, X. N., Xu, Q., Qian, Z. M. (2006) Effective protection of Terminalia catappa L. leaves from damage induced by carbon tetrachloride in liver mitochondria. J. Nutr. Biochem. 17 , 177–182.

    Qian Z. M. , 'Effective protection of Terminalia catappa L. leaves from damage induced by carbon tetrachloride in liver mitochondria ' (2006 ) 17 J. Nutr. Biochem. : 177 -182 .

    • Search Google Scholar
  • Vaca, C. E., Wilhelm, J., Harms-Rihsdahl, M. (1988) Interaction of lipid peroxidation product with DNA. Mutat. Res. Rev. Genet. Toxicol. 195 , 137–149.

    Harms-Rihsdahl M. , 'Interaction of lipid peroxidation product with DNA ' (1988 ) 195 Mutat. Res. Rev. Genet. Toxicol. : 137 -149 .

    • Search Google Scholar
  • Weber, L. W. D., Bull, M., Stampfl, A. (2003) Hepatotoxicity and mechanism of action of haloalkanes: Carbon tetrachloride as a toxicological model. Crit. Rev. Toxicol. 33 , 105–136.

    Stampfl A. , 'Hepatotoxicity and mechanism of action of haloalkanes: Carbon tetrachloride as a toxicological model ' (2003 ) 33 Crit. Rev. Toxicol. : 105 -136 .

    • Search Google Scholar
  • Williams, A. T., Burk, R. F. (1990) Carbon tetrachloride hepatotoxicity: An example of free radicalmediated injury. Semin. Liver Dis. 10 , 279–284.

    Burk R. F. , 'Carbon tetrachloride hepatotoxicity: An example of free radicalmediated injury ' (1990 ) 10 Semin. Liver Dis. : 279 -284 .

    • Search Google Scholar
  • Zimmerman, H. J., Seef, L. B. (1970) Enzymes in hepatic disease. In: Goodley, E. L. (ed.), Diagnostic Enzymology . Philadelphia, pp. 1–38.

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Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

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Acta Biologica Hungarica
Language English
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1950
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Founder Magyar Tudományos Akadémia
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ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)