Acta Biologica Hungarica
Volume/Issue:
Volume 61: Issue 2
Cupric stress induces oxidative damage marked by accumulation of H 2 O 2 and changes to chloroplast ultrastructure in primary leaves of beans ( Phaseolus vulgaris L.)
Authors:
Houda Bouazizi Faculty of Sciences Bizerte Laboratory of Bio-Cell Physiology 7021 Zarzouna Tunisia

Search for other papers by Houda Bouazizi in
Current site
Google Scholar
PubMed Close
,
Hager Jouili Faculty of Sciences Bizerte Laboratory of Bio-Cell Physiology 7021 Zarzouna Tunisia

Search for other papers by Hager Jouili in
Current site
Google Scholar
PubMed Close
,
Anja Geitmann University of Montreal Department of Biological Sciences, Institute Research in Plant Biology 4101 Rue Sherbrooke East Montreal Quebec H1X 2B2 Canada

Search for other papers by Anja Geitmann in
Current site
Google Scholar
PubMed Close
, and
Ezzeddine Ferjani Faculty of Sciences Bizerte Laboratory of Bio-Cell Physiology 7021 Zarzouna Tunisia

Search for other papers by Ezzeddine Ferjani in
Current site
Google Scholar
PubMed Close
Pages:
191–203
Online Publication Date:
01 Jun 2010
Publication Date:
01 Jun 2010
Article Category:
Research Article
DOI:
https://doi.org/10.1556/abiol.61.2010.2.7
Keywords:
Bean; chloroplast; copper; H 2 O 2; oxidative stress
Restricted access

The effect of copper excess (CuSO 4 ) on lipid peroxidation, H 2 O 2 content, and antioxidative enzyme activities was studied in primary leaves of bean seedlings. Fourteen-day-old bean seedlings were cultured in a nutrient solution containing Cu 2+ at various concentrations (50 and 75 μM) for 3 days. Excess of copper significantly increased malondialdehyde content and endogenous H 2 O 2 . This radical accumulated in the intercellular spaces of palisade mesophyll cells. In addition, cupric stress induced changes in antioxidant enzyme activities. GPX (guaiacol peroxidase, EC 1.11.1.7) activity was decreased in 50 μM Cu-stressed leaves whereas 75 μM of CuSOP 4 resulted in an increase of enzyme activity. On the contrary, CAT (catalase, EC 1.11.1.6) activity was stimulated at 50 μM CuSO 4 but unaltered at 75 μM CuSO 4 . Transmission electron microscopy revealed that excess copper induced changes in the ultrastructure of chloroplasts visible in form of a deterioration in the grana structure and the accumulation and swelling of starch grains in the stroma.

  • Aebi, H. (1984) Catalase in vitro . Methods in Enzymology 105 , 121–126.

    Aebi H. , 'Catalase in vitro ' (1984 ) 105 Methods in Enzymology : 121 -126 .

  • Alaoui-sossé, B., Genet, P., Vinit-Dumand, F., Toussaint, M. L., Epron, D., Badot, P. M. (2004) Effect of Cu on growth in cucumber plants (Cucumis sativus) and its relationships with carbohydrate accumulation and changes in ion contents. Plant Sci. 166 , 1213–1218.

    Badot P. M. , 'Effect of Cu on growth in cucumber plants (Cucumis sativus) and its relationships with carbohydrate accumulation and changes in ion contents ' (2004 ) 166 Plant Sci. : 1213 -1218 .

    • Search Google Scholar

  • Alvarez, M. E., Penel, R. I., Meijer, P. J., Ishikaw, A., Dixon, R. A., Lamb, C. (1998) Reactive oxygen intermediate a systemic signal network in the establishment of plant immunity. Cell 92 , 773–784.

    Lamb C. , 'Reactive oxygen intermediate a systemic signal network in the establishment of plant immunity ' (1998 ) 92 Cell : 773 -784 .

    • Search Google Scholar

  • Baccouche, S., Chaoui, A., El Ferjani, E. (1998) Nickel induced oxidative damage and antioxidant responses in Zea mays shoots. Plant Physiol. Biochem. 36 , 689–694.

    Ferjani E. , 'Nickel induced oxidative damage and antioxidant responses in Zea mays shoots ' (1998 ) 36 Plant Physiol. Biochem. : 689 -694 .

    • Search Google Scholar

  • Barceló, J., Vazquez, M. D., Poschenrieder, C. H. (1988) Structural and ultrastructural disorder in cadmium-treated bush plants ( Phaseolus vulgaris L.). New Physiol. 108 , 37–49.

    Poschenrieder C. H. , 'Structural and ultrastructural disorder in cadmium-treated bush plants (Phaseolus vulgaris L.) ' (1988 ) 108 New Physiol. : 37 -49 .

    • Search Google Scholar

  • Bolwell, G. P., Wojtaszek, P. (1997) Mechanism for generation of reactive oxygen species in plant defence-a broad perspective. Physiol. Mol. Plant Pathol. 51 , 347–366.

    Wojtaszek P. , 'Mechanism for generation of reactive oxygen species in plant defence-a broad perspective ' (1997 ) 51 Physiol. Mol. Plant Pathol. : 347 -366 .

    • Search Google Scholar

  • Bouazizi, H., Jouili, H., Geitmann, A., El Ferjani, E. (2008) Effect of copper excess on H2O2 accumulation and peroxidase activities in bean roots. Acta Biol. Hung. 59 , 233–245.

    Ferjani E. , 'Effect of copper excess on H2O2 accumulation and peroxidase activities in bean roots ' (2008 ) 59 Acta Biol. Hung. : 233 -245 .

    • Search Google Scholar

  • Bouazizi, H. (2008) Modifications biochimiques et structurales induites par le stress cuprique au niveau des parois cellulaires du haricot vert (Phaseolus vulgaris L. ). PhD thesis, University of 7 th November at Carthage, Tunisia.

    Bouazizi H. , '', in Modifications biochimiques et structurales induites par le stress cuprique au niveau des parois cellulaires du haricot vert , (2008 ) -.

  • Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Annal. Biochem. 72 , 248–258.

    Bradford M. M. , 'A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding ' (1976 ) 72 Annal. Biochem. : 248 -258 .

    • Search Google Scholar

  • Cakmak, I., Horst, W. J. (1991) Effect of aluminium on net efflux on nitrate and potassium from roots tips of soybean ( glycine max L.). Plant Physiol. 13 , 400–403.

    Horst W. J. , 'Effect of aluminium on net efflux on nitrate and potassium from roots tips of soybean (glycine max L.) ' (1991 ) 13 Plant Physiol. : 400 -403 .

    • Search Google Scholar

  • Chen, L. M., Lin, C. C., Kao, C. H. (2000) Cu toxicity in rice seedlings: changes in antioxidative enzyme activities, H 2 O 2 level and cell wall peroxidase activity in roots. Botan. Bull. Acad. Sinica 41 , 99–103.

    Kao C. H. , 'Cu toxicity in rice seedlings: changes in antioxidative enzyme activities, H2O2 level and cell wall peroxidase activity in roots ' (2000 ) 41 Botan. Bull. Acad. Sinica : 99 -103 .

    • Search Google Scholar

  • Devi, S. R., Prasad, M. N. V. (2004) Membrane lipid alterations in heavy metal exposed plants. In: Prasad, M. N. V. Heavy Metal Stress in Plants — From Biomolecules to Ecosystems . Second Edition. Springer, Berlin. pp. 127–144.

    Prasad M. N. V. , '', in Heavy Metal Stress in Plants — From Biomolecules to Ecosystems , (2004 ) -.

  • Demidchik, V., Sokolik, A., Yurin, V. (1997) The effects of Cu 2+ on ion transport systems of the plant cell membrane. J. Plant Physiol. 114 , 1313–1325.

    Yurin V. , 'The effects of Cu2+ on ion transport systems of the plant cell membrane ' (1997 ) 114 J. Plant Physiol. : 1313 -1325 .

    • Search Google Scholar

  • Djebali, W., Zarrouk, M., Brouquisse, R., El Kahoui, S., Limann, F., Chaibi, W., Ghorbel, M. H. (2005) Ultrastructure and lipid alterations induced by cadmium in tomato ( Lycopersion esculentum ) chloroplast membranes. Plant Biol. 7 , 1801–1810.

    Ghorbel M. H. , 'Ultrastructure and lipid alterations induced by cadmium in tomato (Lycopersion esculentum) chloroplast membranes ' (2005 ) 7 Plant Biol. : 1801 -1810 .

    • Search Google Scholar

  • Dučić, T., Polle, A. (2005) Transport and detoxification of manganese and copper in plants. Braz. J. Plant Physiol. 17 , 103–112.

    Polle A. , 'Transport and detoxification of manganese and copper in plants ' (2005 ) 17 Braz. J. Plant Physiol. : 103 -112 .

    • Search Google Scholar

  • Eleftheriou, E. P., Karataglis, S. (1989) Ultrastructural and morphological characteristics of cultivated wheat growing on copper-polluted field. Bot. Acta 102 , 134–140.

    Karataglis S. , 'Ultrastructural and morphological characteristics of cultivated wheat growing on copper-polluted field ' (1989 ) 102 Bot. Acta : 134 -140 .

    • Search Google Scholar

  • Elstner, E. F., Wagner, G. A., Schütz, W. (1988) Activated oxygen in green plants in relation to stress situations. Curr. Topics Plant Biochem. 36 , 873–877.

    Schütz W. , 'Activated oxygen in green plants in relation to stress situations ' (1988 ) 36 Curr. Topics Plant Biochem. : 873 -877 .

    • Search Google Scholar

  • Fielding, J. L., Hall, J. L. (1978) A biochemical and cytochemical study of peroxidase activity in roots of Pisum sativum . J. Exp. Bot. 29 , 979–986.

    Hall J. L. , 'A biochemical and cytochemical study of peroxidase activity in roots of Pisum sativum ' (1978 ) 29 J. Exp. Bot. : 979 -986 .

    • Search Google Scholar

  • Gora, L., Clijsters, H. (1985) Effects of copper and zinc on the ethylene metabolism in Phaseolus vulgaris L. In: H. Clijsters, M. De Proft, R. Marcelle, M. Van Poucke (eds), Biochemical and physiological aspects of ethylene production in lower and higher plants . Kluwer Academic Publishers Dordrecht. pp. 219–228.

  • Halliwell, B., Gutteridge, J. M. C. (1984) Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem. J. 219 , 1–14.

    Gutteridge J. M. C. , 'Oxygen toxicity, oxygen radicals, transition metals and disease ' (1984 ) 219 Biochem. J. : 1 -14 .

    • Search Google Scholar

  • Halliwell, B., Gutteridge, J. M. C. (1990) Role of free radicals and catalytic metalions in human disease; an overview. Meth. Enzymol. 186 , 1–85.

    Gutteridge J. M. C. , 'Role of free radicals and catalytic metalions in human disease; an overview ' (1990 ) 186 Meth. Enzymol. : 1 -85 .

    • Search Google Scholar

  • Herren, T., Feller, U. (1997) Influence of increased zinc levels on phloem transport in wheat shoots. J. Plant Physiol. 150 , 228–231.

    Feller U. , 'Influence of increased zinc levels on phloem transport in wheat shoots ' (1997 ) 150 J. Plant Physiol. : 228 -231 .

    • Search Google Scholar

  • Karlsson, M., Melzer, M., Prokhorenko, I., Johansson, T., Wingsle, G. (2005) Hydrogen peroxide and expression of hipl-superoxide dismutase are associated with the development of secondary cell walls in Zinnia elegans . J. Exp. Bot. 56 , 2085–2093.

    Wingsle G. , 'Hydrogen peroxide and expression of hipl-superoxide dismutase are associated with the development of secondary cell walls in Zinnia elegans ' (2005 ) 56 J. Exp. Bot. : 2085 -2093 .

    • Search Google Scholar

  • Lindberg, S., Griffiths, G. (1993) Aluminum effects on ATPase activity and lipid composition of plasma membranes in sugar beet roots. J. Exp. Bot. 44 , 1543–1550.

    Griffiths G. , 'Aluminum effects on ATPase activity and lipid composition of plasma membranes in sugar beet roots ' (1993 ) 44 J. Exp. Bot. : 1543 -1550 .

    • Search Google Scholar

  • Lombardi, L., Sebastaiani, L. (2005) Copper toxicity in Prunus cerasifera : growth and antioxidant enzymes responses of in vitro grown plants. Plant Sci. 168 , 797–802.

    Sebastaiani L. , 'Copper toxicity in Prunus cerasifera: growth and antioxidant enzymes responses of in vitro grown plants ' (2005 ) 168 Plant Sci. : 797 -802 .

    • Search Google Scholar

  • Maksymiec, W., Russa, R., Urbanik-Sypniezska, T., Baszynski, T. (1992) Changes in acyl lipid and fatty acid composition in thylakoids of copper non-tolerant spinach exposed to excess copper. J. Plant Physiol. 140 , 52–55.

    Baszynski T. , 'Changes in acyl lipid and fatty acid composition in thylakoids of copper non-tolerant spinach exposed to excess copper ' (1992 ) 140 J. Plant Physiol. : 52 -55 .

    • Search Google Scholar

  • Maksymiec, W. (2007) Signaling responses in plants to heavy metal stress. Acta Physiol. Plant 29 , 177–187.

    Maksymiec W. , 'Signaling responses in plants to heavy metal stress ' (2007 ) 29 Acta Physiol. Plant : 177 -187 .

    • Search Google Scholar

  • Mazhoudi, S., Chaoui, A., Ghorbal, M. H., El Ferjani, E. (1997) Response of antioxidant enzymes to excess copper in tomato ( Lycopersion esculentum , Mill.). Plant Sci. 127 , 182–186.

    Ferjani E. , 'Response of antioxidant enzymes to excess copper in tomato (Lycopersion esculentum, Mill.) ' (1997 ) 127 Plant Sci. : 182 -186 .

    • Search Google Scholar

  • Mithöfer, A., Schulze, B., Boland, W. (2004) Biotic and heavy metal stress response in plants: evidence for common signals. FEBS Letters 566 , 1–5.

    Boland W. , 'Biotic and heavy metal stress response in plants: evidence for common signals ' (2004 ) 566 FEBS Letters : 1 -5 .

    • Search Google Scholar

  • Molas, J. (1997) Changes in morphological and anatomical structure of cabbage ( Brassica oleracea L.) outer leaves and ultrastructure of their chloroplasts caused by in vitro excess of nickel. Photosynthetica 34 , 513–522.

    Molas J. , 'Changes in morphological and anatomical structure of cabbage (Brassica oleracea L.) outer leaves and ultrastructure of their chloroplasts caused by in vitro excess of nickel ' (1997 ) 34 Photosynthetica : 513 -522 .

    • Search Google Scholar

  • Orozo-Cárdenas, M. L., Narváez-Vásquez, J., Ryan, C. A. (2001) Hydrogen peroxide acts as a second messenger for the induction of defense genes in tomato plants in responses to wounding, systemin and methyl jasmonate. Plant Cell 13 , 179–191.

    Ryan C. A. , 'Hydrogen peroxide acts as a second messenger for the induction of defense genes in tomato plants in responses to wounding, systemin and methyl jasmonate ' (2001 ) 13 Plant Cell : 179 -191 .

    • Search Google Scholar

  • Quartacci, M. F., Pinzino, C., Sgherri, C. L. M., Vecchia, F. D., Navari-Izzo, F. (2000) Growth in excess copper induces changes in the lipid composition and fluidity of PS II enriched membranes in wheat. Physiol. Plant 108 , 87–93.

    Navari-Izzo F. , 'Growth in excess copper induces changes in the lipid composition and fluidity of PS II enriched membranes in wheat ' (2000 ) 108 Physiol. Plant : 87 -93 .

    • Search Google Scholar

  • Panou-Filotheou, H., Bosablidis, M., Karataglis, S. (2001) Effects of copper toxicity on leaves of Origanum vulgare subsp. Hirtum Ann. Bot. 88 , 207–214.

    Karataglis S. , 'Effects of copper toxicity on leaves of Origanum vulgare subsp ' (2001 ) 88 Hirtum Ann. Bot. : 207 -214 .

    • Search Google Scholar

  • Penel, C., Carpin, S., Crevecoeur, M., Simon, P., Greppin, H. (2000) Binding of peroxidases to Ca 2+ -pectate: possible significance for peroxidase function in cell wall. Plant Peroxidase Newslett. 14 , 33–40.

    Greppin H. , 'Binding of peroxidases to Ca2+-pectate: possible significance for peroxidase function in cell wall ' (2000 ) 14 Plant Peroxidase Newslett. : 33 -40 .

    • Search Google Scholar

  • Peng, H. Y., Yang, X., Tian, S. (2005) Accumulation and ultrastructural distribution of copper in Elsholtzia splendens . J. Zhejiang Univ. 6 , 311–318.

    Tian S. , 'Accumulation and ultrastructural distribution of copper in Elsholtzia splendens ' (2005 ) 6 J. Zhejiang Univ. : 311 -318 .

    • Search Google Scholar

  • Prasad, M. N. V., Strazalka, K. (1999) Impact of heavy metals on photosynthesis. In: Prasad, M. N. V., Hagemeyer, J. (eds), Heavy metal stress in plants . Springer Publishers, Berlin, pp. 117–138.

    Strazalka K. , '', in Heavy metal stress in plants , (1999 ) -.

  • Ranieri, A., Castagna, A., Pacini, J., Baldan, B., Mensuali, S., Soldatin, G. F. (2003) Early production and scavenging of hydrogen peroxide in the apoplast of sunflower plants exposed to ozone. J. Exp. Bot. 54 , 2529–2540.

    Soldatin G. F. , 'Early production and scavenging of hydrogen peroxide in the apoplast of sunflower plants exposed to ozone ' (2003 ) 54 J. Exp. Bot. : 2529 -2540 .

    • Search Google Scholar

  • Reichman, S. M. (2002) The responses of plants to metal toxicity: a review focusing on copper, manganese and zinc. Australian Minerals Energy Environm. Found. 14 , 1–54.

    Reichman S. M. , 'The responses of plants to metal toxicity: a review focusing on copper, manganese and zinc ' (2002 ) 14 Australian Minerals Energy Environm. Found. : 1 -54 .

    • Search Google Scholar

  • Ros, R., Cooke, D. T., Burden R. S., James, C. S. (1990) Effect of the herbicide MCPA and the heavy metals, cadmium and nickel on the lipid composition Mg-ATPase activity and fluidity of plasma membrane from rice, Oryza sativa c.v. Babia shoots. J. Exp. Bot. 41 , 457–462.

    James C. S. , 'Effect of the herbicide MCPA and the heavy metals, cadmium and nickel on the lipid composition Mg-ATPase activity and fluidity of plasma membrane from rice, Oryza sativa c.v. Babia shoots ' (1990 ) 41 J. Exp. Bot. : 457 -462 .

    • Search Google Scholar

  • Ros, R., Morales, A., Segura, J., Picazo, I. (1992) In vivo and in vitro effects to nickel and cadmium on the plasmalemma ATP ase from rice ( Oryza sativa L.) shoots and roots. Plant Sci. 83 , 1–6.

    Picazo I. , 'In vivo and in vitro effects to nickel and cadmium on the plasmalemma ATP ase from rice (Oryza sativa L.) shoots and roots ' (1992 ) 83 Plant Sci. : 1 -6 .

    • Search Google Scholar

  • Sandmann, G., Böger, P. (1980) Copper-mediated lipid peroxidation processes in photosynthetic membranes. Plant Physiol. 66 , 797–800.

    Böger P. , 'Copper-mediated lipid peroxidation processes in photosynthetic membranes ' (1980 ) 66 Plant Physiol. : 797 -800 .

    • Search Google Scholar

  • Sanita, Di Troppi, L., Fossati, F., Mustti, R., Mikerezi, I., Favali, M. A. (2002) Effects of hexavalent chromium on maize, tomato, and cauliflower plants. J. Plant Nutr. 25 , 701–717.

    Favali M. A. , 'Effects of hexavalent chromium on maize, tomato, and cauliflower plants ' (2002 ) 25 J. Plant Nutr. : 701 -717 .

    • Search Google Scholar

  • Schützendübel, A., Polle, A. (2001) Plant responses to abiotic stress: heavy metal-induced oxidative stress and protection by mycorrhization. J. Exp. Bot. 53 , 1351–1365.

    Polle A. , 'Plant responses to abiotic stress: heavy metal-induced oxidative stress and protection by mycorrhization ' (2001 ) 53 J. Exp. Bot. : 1351 -1365 .

    • Search Google Scholar

  • Sergiev, I., Alexieva, V., Karanov, E. (1997) Effect of spermine, atrazine and combination between them on some endogenous protective systems and stress markers in plants. Comt. Rend. Acad. Bulg. Sci. 51 , 121–124.

    Karanov E. , 'Effect of spermine, atrazine and combination between them on some endogenous protective systems and stress markers in plants ' (1997 ) 51 Comt. Rend. Acad. Bulg. Sci. : 121 -124 .

    • Search Google Scholar

  • Shu, W. S., Ye, Z. H., Lan, C. Y., Zhang, Z. Q., Wong, M. H. (2002) Lead, Zinc and copper accumulation and tolerance in populations of Paspalum distichum and Cynodon dactylon . Environ. Pollut. 120 , 445–453.

    Wong M. H. , 'Lead, Zinc and copper accumulation and tolerance in populations of Paspalum distichum and Cynodon dactylon ' (2002 ) 120 Environ. Pollut. : 445 -453 .

    • Search Google Scholar

  • Velikova, V., Yordanov, I., Edreva, Q. (2000) Oxidative and some antioxidant systems in acid raintreated bean plants protection role of exogenous polyamines. Plant Sci. 151 , 59–66.

    Edreva Q. , 'Oxidative and some antioxidant systems in acid raintreated bean plants protection role of exogenous polyamines ' (2000 ) 151 Plant Sci. : 59 -66 .

    • Search Google Scholar

  • Wang, H., Shan, X., Wen, B., Zhang, S., Wang, Z. (2004) Responses of antioxidative enzymes to accumulation of copper in copper hyperaccumulator of Commoelina communis . Arch. Environ. Contam. Toxicol. 47 , 185–192.

    Wang Z. , 'Responses of antioxidative enzymes to accumulation of copper in copper hyperaccumulator of Commoelina communis ' (2004 ) 47 Arch. Environ. Contam. Toxicol. : 185 -192 .

    • Search Google Scholar

  • Wecks, J. E. J., Clijsters, H. M. M. (1997) Zn phytotoxicity induces oxidative stress in primary leaves of Phaseolus vulgaris . Plant Physiol. Biochem. 35 , 405–410.

    Clijsters H. M. M. , 'Zn phytotoxicity induces oxidative stress in primary leaves of Phaseolus vulgaris ' (1997 ) 35 Plant Physiol. Biochem. : 405 -410 .

    • Search Google Scholar

  • Yruela, I. (2005) Copper in plants. Braz. J. Plant Physiol. 17 , 145–156.

    Yruela I. , 'Copper in plants ' (2005 ) 17 Braz. J. Plant Physiol. : 145 -156 .

  • Yurekli, F., Porgali, B. Z. (2006) The effects of excessive exposure to copper in bean plants. Acta Biol. Cracoviensia Series Botanica 48 , 7–13.

    Porgali B. Z. , 'The effects of excessive exposure to copper in bean plants ' (2006 ) 48 Acta Biol. Cracoviensia Series Botanica : 7 -13 .

    • Search Google Scholar
  • Collapse
  • Expand
Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X

Senior editors

Editor(s)-in-Chief: Elekes, Károly

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

Acta Biologica Hungarica
P.O. Box 35
H-8237 Tihany, Hungary
Phone: (36 87) 448 244 ext. 103
Fax: (36 87) 448 006
E-mail: elekes@tres.blki.hu

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture
  • Biology and Environmental Sciences
  • Elsevier BIOBASE
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Acta Biologica Hungarica
Language English
Size  
Year of
Foundation		 1950
Publication
Programme		 changed title
Volumes
per Year		  
Issues
per Year		  
Founder Magyar Tudományos Akadémia
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Apr 2024 13 0 0
May 2024 7 0 0
Jun 2024 30 0 0
Jul 2024 13 0 0
Aug 2024 13 0 0
Sep 2024 23 0 0
Oct 2024 0 0 0