Acta Biologica Hungarica
Volume/Issue:
Volume 61: Issue 2
Physiological responses of Arabidopsis thaliana to the interaction of iron deficiency and nitrogen form
Authors:
Najoua Karray-Bouraoui Campus Universitaire Physiology and Biochemistry of the Salt Tolerance of Plants, Faculty of Sciences of Tunis 2092 Tunis El Manar Tunisia

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Houneida Attia Campus Universitaire Physiology and Biochemistry of the Salt Tolerance of Plants, Faculty of Sciences of Tunis 2092 Tunis El Manar Tunisia

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Manel Maghzaoui Campus Universitaire Physiology and Biochemistry of the Salt Tolerance of Plants, Faculty of Sciences of Tunis 2092 Tunis El Manar Tunisia

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Najoua Msilini Campus Universitaire Physiology and Biochemistry of the Salt Tolerance of Plants, Faculty of Sciences of Tunis 2092 Tunis El Manar Tunisia

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M. Rabhi Center of Biotechnology Technopole of Borj Cédria Laboratory of Adaptation of Plants to Abiotic Stress BP 901 Hammam-lif 2050 Tunisia

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M. Lachaâl Campus Universitaire Physiology and Biochemistry of the Salt Tolerance of Plants, Faculty of Sciences of Tunis 2092 Tunis El Manar Tunisia

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Pages:
204–213
Online Publication Date:
01 Jun 2010
Publication Date:
01 Jun 2010
Article Category:
Research Article
DOI:
https://doi.org/10.1556/abiol.61.2010.2.8
Keywords:
Arabidopsis thaliana ; iron nutrition; ammonium; nitrate; nitrogen form
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Physiological responses of Arabidopsis thaliana to the interaction of iron deficiency and nitrogen form were studied using plants grown in hydroponics. Thirty-three-day-old seedlings were submitted to four treatments for 7 days: NO 3 + 5 μM Fe; NO 3 + 0.1 μM Fe; NH 4 + 5 μM Fe and NH 4 + 0.1 μM Fe. Leaf growth and chlorophyll content were highest in NO 3 -fed, Fe sufficient plants, but were strongly diminished by Fe deficiency under nitric nutrition, and by ammoniacal nutrition independently of Fe regime. However, the leaves of NH 4 -fed plants presented a higher Fe content than those of Fe sufficient, NO 3 -fed plants. Thus, leaf chlorosis of NH 4 -fed in plant did not depend on Fe availability, and seemed to be due to another factor. Root acidification capacity and Fe-chelate reductase (FCR) activity were also dependent on N form. The medium was acidified under ammoniacal regime and alkalinized under nitric regime regardless of Fe level. FCR activity stimulation in response to Fe deficiency was observed only in NO 3− fed plants. In addition, both N form and Fe level induced antioxidant responses in rosette leaves. Ammoniacal regime increased both peroxidase expression and anthocyanin accumulation, whereas Fe deficiency enhanced superoxide dismutase expression.

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Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X

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Acta Biologica Hungarica
Language English
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