Acta Biologica Hungarica
Volume/Issue:
Volume 61: Issue 2
Cryopreservation of the non-dormant orthodox seeds of Ulmus glabra
Author: P. Chmielarz 1
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  • 1 Polish Academy of Sciences Institute of Dendrology Parkowa 5 62-035 Kórnik Poland
DOI:
https://doi.org/10.1556/abiol.61.2010.2.10
Pages:
224–233
Publication Date:
01 Jun 2010
Online Publication Date:
01 Jun 2010
Article Category:
Research Article
Restricted access

In order to evaluate the feasibility of cryopreservation of Wych elm ( Ulmus glabra Huds.) seeds, we evaluated the seeds sensitivity to extreme desiccation and/or the ultra-low temperature of liquid nitrogen (LN; −196 °C). We also determined the critical water content (WC) of desiccated seeds and the high-moisture freezing limit of seeds desiccated or moistened to various WCs and frozen for 24 h or up to two years in LN. Germination tests revealed no critical WC for seeds to 0.03 g H 2 O g −1 dry mass, g g −1 . Seeds tolerated freezing in LN within safe ranges of WC 0.03–0.21 g g −1 (nuts). Seeds desiccated to the safe WC and stored in LN for two years had similar germination as seeds stored at −3 °C for two years. Therefore, long-term cryopreservation of U. glabra seeds in gene banks is feasible.

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Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X
Keywords:
Ulmus glabra ; desiccation; liquid nitrogen; seed storage; water content

Senior editors

Editor(s)-in-Chief: Elekes, Károly

Editorial Board

      Csányi, Vilmos (Göd)
      Dudits, Dénes (Szeged)
      Falus, András (Budapest)
      Fischer, Ernő (Pécs)
      Gábriel, Róbert (Pécs)
      Gulya, Károly (Szeged)
      Gulyás, Balázs (Stockholm)
      Hajós, Ferenc (Budapest)
      Hámori, József (Budapest)
      Heszky, László (Gödöllő)
      Hideg, Éva (Szeged)
      E. Ito (Sanuki)
      Janda, Tibor (Martonvásár)
      Kavanaugh, Michael P. (Missoula)
      Kása, Péter (Szeged)
      Klein, Éva (Stockholm)
      Kovács, János (Budapest)
      Brigitte Mauch-Mani (Neuchâtel)
      Nässel, Dick R. (Stockholm)
      Nemcsók, János (Szeged)
      Péczely, Péter (Gödöllő)
      Roberts, D. F. (Newcastle-upon-Tyne)
      Sakharov, Dimitri A. (Moscow)
      Singh, Meharvan (Fort Worth)
      Sipiczky, Mátyás (Debrecen)
      Szeberényi, József (Pécs)
      Székely, György (Debrecen)
      Tari, Irma (Szeged)
      Vágvölgyi, Csaba (Szeged),
      L. Zaborszky (Newark)

 

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Acta Biologica Hungarica
Language English
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Founder Magyar Tudományos Akadémia
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ISSN 0236-5383 (Print)
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