Acta Biologica Hungarica
Volume/Issue:
Volume 61: Issue 3
High frequency plant regeneration from mature seed of elite, recalcitrant Malaysian indica rice ( Oryza sativa L.) CV. MR 219
Authors: P. Sivakumar 1 , Y. Law 1 , C. Ho 2 , and Jennifer Harikrishna 1
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  • 1 University of Malaya Centre for Research in Biotechnology for Agriculture (CEBAR) and Institute of Biological Sciences, Faculty of Science 50603 Kuala Lumpur Malaysia
  • 2 University Putra Malaysia Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences 43400 UPM Serdang, Selangor Malaysia
DOI:
https://doi.org/10.1556/abiol.61.2010.3.7
Page Count:
313–321
Publication Date:
01 Sep 2010
Online Publication Date:
19 Aug 2010
Article Category:
Research Article
Restricted access

An efficient in vitro plant regeneration system was established for elite, recalcitrant Malaysian indica rice, Oryza sativa L. CV. MR 219 using mature seeds as explant on Murashige and Skoog and Chu N6 media containing 2,4-dichlorophenoxy acetic acid and kinetin either alone or in different combinations. L-proline, casein hydrolysate and L-glutamine were added to callus induction media for enhancement of embryogenic callus induction. The highest frequency of friable callus induction (84%) was observed in N6 medium containing 2.5 mg l −1 2,4-dichlorophenoxy acetic acid, 0.2 mg l −1 kinetin, 2.5 mg l −1 L-proline, 300 mg l −1 casein hydrolysate, 20 mg l −1 L-glutamine and 30 g l −1 sucrose under culture in continuous lighting conditions. The maximum regeneration frequency (71%) was observed, when 30-day-old N6 friable calli were cultured on MS medium supplemented with 3 mg l −1 6-benzyl aminopurine, 1 mg l −1 naphthalene acetic acid, 2.5 mg l −1 L-proline, 300 mg l −1 casein hydrolysate and 3% maltose. Developed shoots were rooted in half strength MS medium supplemented with 2% sucrose and were successfully transplanted to soil with 95% survival. This protocol may be used for other recalcitrant indica rice genotypes and to transfer desirable genes in to Malaysian indica rice cultivar MR219 for crop improvement.

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Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X
Keywords:
Callus induction; Oryza sativa ssp. indica; mature seeds; plant regeneration; recalcitrant

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