Acta Biologica Hungarica
Volume/Issue:
Volume 61: Issue 3
The Plackett-Burman design in optimization of media components for biomass production of Lactobacillus rhamnosus OXY
Authors: A. Waśko 1 , Monika Kordowska-Wiater 1 , M. Podleśny 1 , Magdalena Polak-Berecka 1 , Z. Targoński 1 , and Agnieszka Kubik-Komar 2
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  • 1 University of Life Sciences Department of Biotechnology, Human Nutrition and Food Commodities Lublin Poland
  • 2 University of Life Sciences Department of Applied Mathematics and Computer Science Lublin Poland
DOI:
https://doi.org/10.1556/abiol.61.2010.3.10
Page Count:
344–355
Publication Date:
01 Sep 2010
Online Publication Date:
19 Aug 2010
Article Category:
Research Article
Restricted access

The central composite design was developed to search for an optimal medium for the growth of Lactobacillus rhamnosus OXY. The effect of various media components, such as carbon sources, simple and complex nitrogen sources, mineral agents, and growth factors (vitamins B, amino acids) was examined. The first-order model based on Plackett-Burman design showed that glucose, sodium pyruvate, meat extract and mineral salts significantly influenced the growth of the examined bacteria. The second-order polynomial regression confirmed that maximum biomass production could be achieved by the combination of glucose (12.38 g/l), sodium pyruvate (3.15 g/l), meat extract (4.08 g/l), potassium phosphate (1.46 g/l), sodium acetate (3.65 g/l) and ammonium citrate (1.46 g/l).The validation of the predicted model carried out in bioreactor conditions confirmed the usefulness of the new medium for the culture of L. rhamnosus OXY in large scale. The optimal medium makes the culture of the probiotic bacterium L. rhamnosus OXY more cost effective.

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Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X
Keywords:
Lactobacillus ; biomass; growth; optimization; response surface methodology

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