Acta Biologica Hungarica
Volume/Issue:
Volume 61: Issue 4
Effect of fungicide Euparen Multi (Tolylfluanid) on the induction of chromosomal aberations in cultivated bovine lymphocytes
Authors: Martina Orosová 1 , Beáta Holečková 2 , Katarína Šiviková 2 , and J. Dianovský 2
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  • 1 Slovak Academy of Sciences Parasitological Institute Hlinkova 3 040 01 Košice Slovak Republic
  • 2 University of Veterinary Medicine Institute of Genetics Komenského 73 041 81 Košice Slovak Republic
DOI:
https://doi.org/10.1556/abiol.61.2010.4.5
Page Count:
411–422
Publication Date:
01 Dec 2010
Online Publication Date:
26 Nov 2010
Article Category:
Research Article
Restricted access

The effect of the fungicide Euparen Multi (containing 50% tolylfluanid) was investigated on the induction of chromosomal aberrations (CA) in cultured bovine peripheral lymphocytes. Cultures from two healthy donors were treated with tolylfluanid-based fungicide at concentrations ranging from 1.7 to 17.5 μg/ml for the last 24 and 48 hours of cultivation. Conventional cytogenetic method (CA assay) with Giemsa staining as well as fluorescence in situ hybridization (FISH) with whole bovine chromosomes 1 and 5 painting probes were used in the experiment. In the CA assay, no clastogenic effect of the fungicide was found after Euparen Multi treatment for 24 hours. On the contrary, significant elevation in polyploidy induction was observed with dose-dependence in one of the donors. Using prolonged time of exposure to the fungicide (the last 48 h of the cultivation), a slight clastogenic effect was detected at the doses of 8.75 and 17.5 μg/ml (P < 0.05, P < 0.01, respectively) in donor 1 and at the dose of 8.75 μg/ml (P < 0.05) in donor 2. The highest doses tested caused reduction of the mitotic indices (MI) (P < 0.05, P < 0.01) in both donors as well as both treatment times. The evaluation of stable structural aberrations in lymphocytes by two-colour FISH (48 h exposure) using bovine chromosome painting probes revealed the presence of nonreciprocal translocations at two examined concentrations (3.5 μg/ml and 8.75 μg/ml).

  • Australian Pesticides & Veterinary Medicines Authority (APVMA). (2000) Public Release Summary on Evaluation of the New Active Tolylfluanid in the Product Euparen Multi 500 WG Fungicide, 1–25.

  • Baša Češnik, H., Gregorčič, A., Velikonja Bolta, Š., Kmecl, V. (2006) Monitoring of pesticide residuem in apples, lettuce and potato of Slovene origin, 2001–2004. Food Addit. Contam. 23, 164–173.

    Kmecl V. , 'Monitoring of pesticide residuem in apples, lettuce and potato of Slovene origin, 2001–2004 ' (2006 ) 23 Food Addit. Contam. : 164 -173.

    • Search Google Scholar

  • Bellas, J. (2006) Comparative toxicity of alternative antifouling biocides on embryos and larvae of marine invertebrates. Sci. Total. Environ. 367, 573–585.

    Bellas J. , 'Comparative toxicity of alternative antifouling biocides on embryos and larvae of marine invertebrates ' (2006 ) 367 Sci. Total. Environ. : 573 -585.

    • Search Google Scholar

  • Bolognesi, C. (2003) Genotoxicity of pesticides: a review of human biomonitoring studies. Mutat. Res. 543, 251–272.

    Bolognesi C. , 'Genotoxicity of pesticides: a review of human biomonitoring studies ' (2003 ) 543 Mutat. Res. : 251 -272.

    • Search Google Scholar

  • Celik, M., Unal, F., Yüzbasioglu, D., Ergün, M. A., Arslan, O., Kasap, R. (2005) In vitro effect of karathane LC (dinocap) on humane lymphocytes. Mutagenesis 20, 101–104.

    Kasap R. , 'In vitro effect of karathane LC (dinocap) on humane lymphocytes ' (2005 ) 20 Mutagenesis : 101 -104.

    • Search Google Scholar

  • Costa, C., Teixeira, J. P., Silva, S., Roma-Torres, J., Coelho, P., Gaspar, J., Alves, M., Laffon, B., Rueff, J., Mayan, O. (2006) Cytogenetic and molecular biomonitoring of a Portuguese population exposed to pesticides. Mutagenesis 21, 343–350.

    Mayan O. , 'Cytogenetic and molecular biomonitoring of a Portuguese population exposed to pesticides ' (2006 ) 21 Mutagenesis : 343 -350.

    • Search Google Scholar

  • Domaracký, M., Rehák, P., Legáth, J., Koppel, J. (2007) Effects of fungicide Euparen Multi (tolylfluanid) on development of preimplantation embryos in mouse. Acta Vet. Brno 76, 209–214.

    Koppel J. , 'Effects of fungicide Euparen Multi (tolylfluanid) on development of preimplantation embryos in mouse ' (2007 ) 76 Acta Vet. Brno : 209 -214.

    • Search Google Scholar

  • Duesberg, P., Rasnick, D. (2000) Aneuploidy, the somatic mutation that makes cancer a species of its own. Cell Motil. Cytoskel 47, 81–107.

    Rasnick D. , 'Aneuploidy, the somatic mutation that makes cancer a species of its own ' (2000 ) 47 Cell Motil. Cytoskel : 81 -107.

    • Search Google Scholar

  • European Food Safety Authority (EFSA) (2005) Conclusion regarding the peer review of the pesticide risk assessment of the active substance tolylfluanid. EFSA Sci. Rep. 29, 1–76.

    'Conclusion regarding the peer review of the pesticide risk assessment of the active substance tolylfluanid ' (2005 ) 29 EFSA Sci. Rep. : 1 -76.

    • Search Google Scholar

  • Environmental Protection Agency (EPA) (2002) Pesticide Fact Sheet, Tolylfluanid, 1–18.

  • Fries, R., Popescu, P. (1999) Cytogenetics and Physical Chromosome Maps. In: Fries, R., Ruvinsky, A. (eds) The Genetics of Cattle. CABI Press, Oxon, UK, pp. 251–261.

    Popescu P. , '', in The Genetics of Cattle , (1999 ) -.

  • Galloway, S. M., Miller, J. E., Armstrong, M. J., Bean, Ch. L., Skopek, T. R., Nichols, W. W. (1998) DNA synthesis as an indirect mechanism of chromosome aberrations: comparison of DNA-reactive and non-DNA-reactive clastogens. Mutat. Res. 400, 169–186.

    Nichols W. W. , 'DNA synthesis as an indirect mechanism of chromosome aberrations: comparison of DNA-reactive and non-DNA-reactive clastogens ' (1998 ) 400 Mutat. Res. : 169 -186.

    • Search Google Scholar

  • Garcia-Repetto, R., Martínez, M., Repetto, M. (1997) Biodisposition study of the organophosphorus pesticide, methyl-parathion. B. Environ. Contam. Tox. 59, 901–908.

    Repetto M. , 'Biodisposition study of the organophosphorus pesticide, methyl-parathion ' (1997 ) 59 B. Environ. Contam. Tox. : 901 -908.

    • Search Google Scholar

  • Hagmar, L., Strömberg, U., Bonassi, S., Hansteen, I. L., Knudsen, L. E., Lindholm, C., Norppa, H. (2004) Impact of types of lymphocyte chromosomal aberrations on human cancer risk: Results from Nordic and Italian cohorts. Cancer Res. 64, 2258–2263.

    Norppa H. , 'Impact of types of lymphocyte chromosomal aberrations on human cancer risk: Results from Nordic and Italian cohorts ' (2004 ) 64 Cancer Res. : 2258 -2263.

    • Search Google Scholar

  • Holečková, B. (2006) Evaluation of the in vitro effect of glyphosate-based herbicide on bovine lymphocytes using chromosome painting. B. Vet. I. Pulawy 50, 533–536.

    Holečková B. , 'Evaluation of the in vitro effect of glyphosate-based herbicide on bovine lymphocytes using chromosome painting ' (2006 ) 50 B. Vet. I. Pulawy : 533 -536.

    • Search Google Scholar

  • Holovská, V., Pistl, J., Kovalkovičová, N. (2007) In vitro effect of pesticides (dichlofluanid, endosulfan, simazine, tolylfluanid and triallate) on proliferative activity of animal derived cell cultures. Acta Biol. Hung. 58, 61–74.

    Kovalkovičová N. , 'In vitro effect of pesticides (dichlofluanid, endosulfan, simazine, tolylfluanid and triallate) on proliferative activity of animal derived cell cultures ' (2007 ) 58 Acta Biol. Hung. : 61 -74.

    • Search Google Scholar

  • Ishidate, M., Jr., Miura, K. F., Sofuni, T. (1998) Chromosome aberration assays in genetic toxicology testing in vitro. Mutat. Res. 404, 167–172.

    Sofuni T. , 'Chromosome aberration assays in genetic toxicology testing in vitro ' (1998 ) 404 Mutat. Res. : 167 -172.

    • Search Google Scholar

  • Kirsch-Volders, M., Vanhauwaert, A., De Boeck, M., Decordier, I. (2002) Importance of detecting numerical versus structural chromosome aberrations. Mutat. Res. 504, 137–148.

    Decordier I. , 'Importance of detecting numerical versus structural chromosome aberrations ' (2002 ) 504 Mutat. Res. : 137 -148.

    • Search Google Scholar

  • Konstantinou, I. K., Albanis, T. A. (2004) Worldwide occurrence and effects of antifouling paint booster biocides in the aquatic environment: a review. Environ. Int. 30, 235–248.

    Albanis T. A. , 'Worldwide occurrence and effects of antifouling paint booster biocides in the aquatic environment: a review ' (2004 ) 30 Environ. Int. : 235 -248.

    • Search Google Scholar

  • Kovalkovičová, N., Pistl, J., Eliaš, D., Jacková, A., Novotný, J., Hlinčíková, S., Legáth, J., Révajová, V., Kováč, G. (2005) Influence of subchronic exposure to fungicide tolylfluanid on humoral and cellmediated immune parameters in sheep immunized with Coxiela vaccine. B. Vet. I. Pulawy 49, 287–292.

    Kováč G. , 'Influence of subchronic exposure to fungicide tolylfluanid on humoral and cellmediated immune parameters in sheep immunized with Coxiela vaccine ' (2005 ) 49 B. Vet. I. Pulawy : 287 -292.

    • Search Google Scholar

  • Lucas, J. N., Tenjin, T., Straume, T., Pinkel, D., Moore, D. II., Litt, M., Gray, J. W. (1989) Rapid human chromosome aberration analysis using fluorescence in situ hybridisation. Int. J. Radiat. Biol. 56, 35–44.

    Gray J. W. , 'Rapid human chromosome aberration analysis using fluorescence in situ hybridisation ' (1989 ) 56 Int. J. Radiat. Biol. : 35 -44.

    • Search Google Scholar

  • Marshall, R., Obe, G. (1998) Application of chromosome painting to clastogenicity testing in vitro. Environ. Mol. Mutagen. 32, 212–222.

    Obe G. , 'Application of chromosome painting to clastogenicity testing in vitro ' (1998 ) 32 Environ. Mol. Mutagen. : 212 -222.

    • Search Google Scholar

  • Mitchell, I. G., Lambert, T. R., Burden, M., Sunderland, J., Porter, R. L., Carlton, J. B. (1995) Is polyploidy an important genotoxic lesion? Mutagenesis 10, 79–83.

    Carlton J. B. , 'Is polyploidy an important genotoxic lesion? ' (1995 ) 10 Mutagenesis : 79 -83.

    • Search Google Scholar

  • Mukherjee, A., Rao, K. V. M., Ramesh, V. S. (2009) Predicted concentrations of biocides from antifouling paints in Visakhapatnam Harbour. J. Environ. Manag. 90, S51–S59.

    Ramesh V. S. , 'Predicted concentrations of biocides from antifouling paints in Visakhapatnam Harbour ' (2009 ) 90 J. Environ. Manag. : S51 -S59.

    • Search Google Scholar

  • Parry, E. M., Parry, J. M., Corso, C., Doherty, A., Haddad, F., Hermine, T. F., Johnson, G., Kayani, M., Quick, E., Warr, T., Williamson, J. (2002) Detection and characterization of mechanisms of action of aneugenic chemicals. Mutagenesis 17, 509–521.

    Williamson J. , 'Detection and characterization of mechanisms of action of aneugenic chemicals ' (2002 ) 17 Mutagenesis : 509 -521.

    • Search Google Scholar

  • Pistl, J., Kovalkovičová, N., Holovská, V., Legáth, J., Mikula, I. (2003) Determination of the immunotoxic potential of pesticides on functional activity of sheep leukocytes in vitro. Toxicology 188, 73–81.

    Mikula I. , 'Determination of the immunotoxic potential of pesticides on functional activity of sheep leukocytes in vitro ' (2003 ) 188 Toxicology : 73 -81.

    • Search Google Scholar

  • Rakitsky, V. N., Koblyakov, V. A., Turusov, V. S. (2000) Nongenotoxic (epigenetic) carcinogens: pesticides as an example. A critical review. Teratogen. Carcin. Mut. 20, 229–240.

    Turusov V. S. , 'Nongenotoxic (epigenetic) carcinogens: pesticides as an example. A critical review ' (2000 ) 20 Teratogen. Carcin. Mut. : 229 -240.

    • Search Google Scholar

  • Rojas, E., Montero, R., Herrera, L. A., Sordo, M., Gonsebatt, M. E., Rodriguez, R., Ostrosky-Wegman, P. (1992) Are mitotic index and lymphocyte proliferation kinetics reproducible endpoints in genetic toxicology testing? Mutat. Res. 282, 283–286.

    Ostrosky-Wegman P. , 'Are mitotic index and lymphocyte proliferation kinetics reproducible endpoints in genetic toxicology testing? ' (1992 ) 282 Mutat. Res. : 283 -286.

    • Search Google Scholar

  • Rubeš, J., Kuchárová, S., Vozdová, M., Musilová, P., Zudová, Z. (1998) Cytogenetic analysis of peripheral lymphocytes in medical personnel by means of FISH. Mutat. Res. 412, 293–298.

    Zudová Z. , 'Cytogenetic analysis of peripheral lymphocytes in medical personnel by means of FISH ' (1998 ) 412 Mutat. Res. : 293 -298.

    • Search Google Scholar

  • Sadlo, S., Szpyrka, E., Jazwa, A., Zawislak, A. (2007) Pesticide residues in fruit and vegetables from southeastern Poland, 2004–2005. Pol. J. Environ. Stud. 16, 313–319.

    Zawislak A. , 'Pesticide residues in fruit and vegetables from southeastern Poland, 2004–2005 ' (2007 ) 16 Pol. J. Environ. Stud. : 313 -319.

    • Search Google Scholar

  • Saunders, D. S., Harper, C. (1994) Pesticides. In: Hayes, A. W. (ed.) Principles and Methods of Toxicology, Third Edition. Raven Press Ltd., New York, pp. 389–415.

    Harper C. , '', in Principles and Methods of Toxicology , (1994 ) -.

  • Schmidt, C. K., Brauch, J. N. (2008) N-dimethylsulfamide as precursor for N-nitrosodimethylamine (NDMA) formation upon ozonation and its fate during drinking water treatment. Environ. Sci. Technol. 42, 6340–6346.

    Brauch J. N. , 'N-dimethylsulfamide as precursor for N-nitrosodimethylamine (NDMA) formation upon ozonation and its fate during drinking water treatment ' (2008 ) 42 Environ. Sci. Technol. : 6340 -6346.

    • Search Google Scholar

  • Schuler, M., Muehlbauer, P., Guzzie, P., Eastmond, D. A. (1999) Noscapine hydrochloride disrupts the mitotic spindle in mammalian cells and induced aneuploidy as well as polyploidy in cultured human lymphocytes. Mutagenesis 14, 51–56.

    Eastmond D. A. , 'Noscapine hydrochloride disrupts the mitotic spindle in mammalian cells and induced aneuploidy as well as polyploidy in cultured human lymphocytes ' (1999 ) 14 Mutagenesis : 51 -56.

    • Search Google Scholar

  • Shen, Y. J., DeLong, C., Tercé, F., Kute, T., Willingham, M. C., Pettenati, M. J., Cui, Y. (2005) Polyploid formation via chromosome duplication induced by CTP: Phosphocholine cytidylyltransferase deficiency and Bcl-2 overexpression: identification of two novel endogenoud factors. J. Histochem. Cytochem. 53, 725–733.

    Cui Y. , 'Polyploid formation via chromosome duplication induced by CTP: Phosphocholine cytidylyltransferase deficiency and Bcl-2 overexpression: identification of two novel endogenoud factors ' (2005 ) 53 J. Histochem. Cytochem. : 725 -733.

    • Search Google Scholar

  • Sram, R. J., Beskid, O., Rössnerova, A., Rössner, P., Lnenickova, Z., Milcova, A., Solansky, I., Binkova, B. (2007) Environmental exposure to carcinogenic polycyclic aromatic hydrocarbons — The interpretation of cytogenetic analysis by FISH. Toxicol. Lett. 172, 12–20.

    Binkova B. , 'Environmental exposure to carcinogenic polycyclic aromatic hydrocarbons — The interpretation of cytogenetic analysis by FISH ' (2007 ) 172 Toxicol. Lett. : 12 -20.

    • Search Google Scholar

  • Stronati, L., Farris, A., Pacchierotti, F. (2004) Evaluation of chromosome painting to assess the induction and persistence of chromosome aberrations in bone marrow cells of mice treated with benzene. Mutat. Res. 545, 1–9.

    Pacchierotti F. , 'Evaluation of chromosome painting to assess the induction and persistence of chromosome aberrations in bone marrow cells of mice treated with benzene ' (2004 ) 545 Mutat. Res. : 1 -9.

    • Search Google Scholar

  • Šiviková, K., Dianovský, J. (2006) Cytogenetic effect of the technical glyphosate on cultivated bovine peripheral lymphocytes. Int. J. Hyg. Envir. Heal. 209, 15–20.

    Dianovský J. , 'Cytogenetic effect of the technical glyphosate on cultivated bovine peripheral lymphocytes ' (2006 ) 209 Int. J. Hyg. Envir. Heal. : 15 -20.

    • Search Google Scholar

  • Šiviková, K., Holečková, B., Dianovský, J. (2005) Chromosome damage induced by benzene after the use of conventional and FISH chromosome painting. Neoplasma 52, 79–84.

    Dianovský J. , 'Chromosome damage induced by benzene after the use of conventional and FISH chromosome painting ' (2005 ) 52 Neoplasma : 79 -84.

    • Search Google Scholar

  • Šutiaková, I., Kovalkovičová, N., Pistl, J., Novotný, J., Legáth, J., Kováč, G., HlinčÍková, S., Šutiak, V. (2006) Chromosomal aberrations and frequency of micronuclei in sheep subchronically exposed to the fungicide Euparen Multi (tolylfluanid). Ecotox. Environ. Safe. 64, 312–320.

    Šutiak V. , 'Chromosomal aberrations and frequency of micronuclei in sheep subchronically exposed to the fungicide Euparen Multi (tolylfluanid) ' (2006 ) 64 Ecotox. Environ. Safe. : 312 -320.

    • Search Google Scholar

  • Ticha, J., Hajslova, J., Kovalczuk, T., Jech, M., Honzicek, J., Kocourek, V., Lansky, M., Kloutvorova, J., Falta, V. (2007) Safe apples for baby-food production: Survey of pesticide treatment regimes leaving minimum residues. Food Addit. Contam. 24, 605–620.

    Falta V. , 'Safe apples for baby-food production: Survey of pesticide treatment regimes leaving minimum residues ' (2007 ) 24 Food Addit. Contam. : 605 -620.

    • Search Google Scholar

  • Tucker, J. D., Preston, R. J. (1996) Chromosome aberration, micronuclei, aneuploidy, sister chromatid exchanges, and cancer risk assessment. Mutat. Res. 365, 147–159.

    Preston R. J. , 'Chromosome aberration, micronuclei, aneuploidy, sister chromatid exchanges, and cancer risk assessment ' (1996 ) 365 Mutat. Res. : 147 -159.

    • Search Google Scholar

  • Tucker, J. D., Ramsey, M. J., Lee, D. A., Minkler, J. L. (1993) Validation of chromosome painting as a biodosimeter in human peripheral lymphocytes following acute exposure to ionising radiation in vitro. Inter. J. Radiat. Biol. 64, 27–37.

    Minkler J. L. , 'Validation of chromosome painting as a biodosimeter in human peripheral lymphocytes following acute exposure to ionising radiation in vitro ' (1993 ) 64 Inter. J. Radiat. Biol. : 27 -37.

    • Search Google Scholar

  • Tucker, J. D., Morgan, W. F., Awa, A. A., Bauchinger, M., Blakey, D., Cornforth, M. N., Littlefield, L. G., Natarajan, A. T., Shasserre, C. (1995) A proposed system for scoring structural aberrations detected by chromosome painting. Cytogenet. Cell Genet. 68, 211–221.

    Shasserre C. , 'A proposed system for scoring structural aberrations detected by chromosome painting ' (1995 ) 68 Cytogenet. Cell Genet. : 211 -221.

    • Search Google Scholar

  • Walorczyk, S. (2008) Development of a multi-residue method for the determination of pesticides in cereals and dry animal feed using gas chromatography — tandem quadrupole mass spectrometry II. Improvement and extension to new analytes. J. Chromatogr. A 1208, 202–214.

    Walorczyk S. , 'Development of a multi-residue method for the determination of pesticides in cereals and dry animal feed using gas chromatography — tandem quadrupole mass spectrometry II. Improvement and extension to new analytes ' (2008 ) 1208 J. Chromatogr. A : 202 -214.

    • Search Google Scholar

  • Warr, T. J., Parry, E. M., Parry, J. M. (1993) A comparison of two in vitro mammalian cell cytogenetic assay for the detection of mitotic aneuploidy using 10 known or suspected aneugens. Mutat. Res. 287, 498–505.

    Parry J. M. , 'A comparison of two in vitro mammalian cell cytogenetic assay for the detection of mitotic aneuploidy using 10 known or suspected aneugens ' (1993 ) 287 Mutat. Res. : 498 -505.

    • Search Google Scholar
Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X
Keywords:
Tolylfluanid; fungicide; chromosome aberration; mitotic index; FISH

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