Acta Biologica Hungarica
Volume/Issue:
Volume 61: Issue 4
Influence of genotype and explant source on the in vitro regeneration ability of different melon varieties
Authors:
Erzsébet Kiss-Bába Corvinus University of Budapest Department of Genetics and Plant Breeding, Faculty of Horticultural Science Budapest Hungary

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,
Sarolta Pánczél

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,
I. Velich Corvinus University of Budapest Department of Genetics and Plant Breeding, Faculty of Horticultural Science Budapest Hungary

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G. Bisztray Corvinus University of Budapest Department of Genetics and Plant Breeding, Faculty of Horticultural Science Budapest Hungary

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Pages:
498–511
Online Publication Date:
26 Nov 2010
Publication Date:
01 Dec 2010
Article Category:
Research Article
DOI:
https://doi.org/10.1556/abiol.61.2010.4.12
Keywords:
Melon; in vitro ; regeneration; cotyledon; decapitated seedlings
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Nine genotypes of melon (Cucumis melo L.) were selected for the investigation of regeneration. Most of the tested varieties showed regeneration ability on medium containing 0.5 mg l−1 or 1 mg l−1 BA, but following the appearance of shoot buds, only six varieties produced leafy shoots. The effect of combinations of BA with different auxins (IAA, NA, 2,4-D) and ABA in the culture medium on shoot regeneration was tested on cotyledon explants of ‘Hógolyó’ and ‘Hale’s Best’. To establish optimal conditions for the adventitious shoot induction six types of seedling-derived explants were prepared from seedlings of four different ages. The best results for shoot forming capacity were achieved with cotyledons followed by decapitated seedlings and hypocotyls derived from 4-day-old seedlings. Cotyledon segments of ‘Hógolyó’ and ‘Hale’s Best’ were also cultivated on media with different concentrations of IAA and BA supplemented with 0.26 mg l−1 ABA. The highest number of well-formed plantlets was counted for ‘Hógolyó’ on the medium supplemented with 0.9 mg l−1 BA+ 0.6 mg l−1 IAA+ 0.26 mg l−1 ABA. This is the first report on the in vitro regeneration of ‘Hógolyó’ from decapitated seedling and hypocotyl explants and of ‘JavÍtott Zentai’, ‘Muskotály’, ‘Hógolyó’, ‘Tétényi csereshéjú’ and ‘Magyar Kincs’ from cotyledon explants.

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Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X

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Editor(s)-in-Chief: Elekes, Károly

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    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
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    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
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    1. Kavanaugh, Michael P. (Missoula)
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    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
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    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

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Acta Biologica Hungarica
Language English
Size  
Year of
Foundation		 1950
Publication
Programme		 changed title
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Founder Magyar Tudományos Akadémia
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Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
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ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)

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