Acta Biologica Hungarica
Volume/Issue:
Volume 62: Issue 2
Characterization of stress processes of Phaffia rhodozyma stress-resistant mutant
Authors:
E. Horváth University of Pécs Department of General and Environmental Microbiology, Faculty of Sciences Pécs Hungary

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G. Papp University of Pécs Department of General and Environmental Microbiology, Faculty of Sciences Pécs Hungary

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Z. Gazdag University of Pécs Department of General and Environmental Microbiology, Faculty of Sciences Pécs Hungary

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J. Belágyi University of Pécs Institute of Biophysics Pécs Hungary

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Á. Blaskó University of Pécs Department of General and Environmental Microbiology, Faculty of Sciences Pécs Hungary

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J. Deli University of Pécs Department of Biochemistry and Medical Chemistry, Faculty of Medicine Pécs Hungary

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Cs. Vágvölgyi University of Szeged Department of Microbiology, Faculty of Science and Informatics Szeged Hungary

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M. Pesti University of Pécs Department of General and Environmental Microbiology, Faculty of Sciences Pécs Hungary

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Pages:
204–210
Online Publication Date:
09 May 2011
Publication Date:
01 Jun 2011
Article Category:
Research Article
DOI:
https://doi.org/10.1556/abiol.62.2011.2.10
Keywords:
Phaffia rhodozyma ; carotenoid; oxidative stress; EPR; GSH
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A carotenoid-less Phaffia rhodozyma mutant (MCP 325) exhibited significantly higher resistance to oxidative stressors such as menadione, H2O2 and K2Cr2O7 than its astaxanthin-producing parental strain (MCP 324). The absence of carotenoids in the mutant did not explain this phenomenon. The cause of the decreased superoxide, hydroxyl radical and glutathione contents, the increased peroxide concentration and the elevated specific activity of catalase under uninduced conditions may be a second mutation. Peroxide treatment induced specific catalase activity in the mutant but not in the parental strain. Regulation of these processes led to the result that, in spite of the mutations, the two strains exhibited the same multiplication rate and generation time.

  • Anderson, M. E. (1985) Determination of glutathione and glutathione disulfide in biological samples. Methods Enzymol. 113, 548–555.

    Anderson M. E. , 'Determination of glutathione and glutathione disulfide in biological samples ' (1985 ) 113 Methods Enzymol. : 548 -555 .

    • Search Google Scholar

  • Blaskó, Á. (2008) Effect of polar and non-polar carotenoids on Xanthophyllomyces dendrorhous: by EPR. Eur. Biophys. J. 37, 1097–1104.

    Blaskó , 'Effect of polar and non-polar carotenoids on Xanthophyllomyces dendrorhous: by EPR ' (2008 ) 37 Eur. Biophys. J. : 1097 -1104 .

    • Search Google Scholar

  • Cantrell, A. (2003) Singlet oxygen quenching by dietary carotenoids in a model membrane environment. Arch. Biochem. Biophys. 412, 47–54.

    Cantrell A. , 'Singlet oxygen quenching by dietary carotenoids in a model membrane environment ' (2003 ) 412 Arch. Biochem. Biophys. : 47 -54 .

    • Search Google Scholar

  • Fell, J. W. (1999) Separation of strains of the yeasts Xanthophyllomyces dendrorhous and Phaffia rhodozyma based on rDNA IGS and ITS sequence analysis. J. Ind. Microbiol. Biotechnol. 23, 677–681.

    Fell J. W. , 'Separation of strains of the yeasts Xanthophyllomyces dendrorhous and Phaffia rhodozyma based on rDNA IGS and ITS sequence analysis ' (1999 ) 23 J. Ind. Microbiol. Biotechnol. : 677 -681 .

    • Search Google Scholar

  • Gazdag, Z. (2003) Chromate tolerance caused by reduced hydroxyl radical production and decreased glutathione reductase activity in Schizosaccharomyces pombe. J. Basic Microbiol. 43, 96–103.

    Gazdag Z. , 'Chromate tolerance caused by reduced hydroxyl radical production and decreased glutathione reductase activity in Schizosaccharomyces pombe ' (2003 ) 43 J. Basic Microbiol. : 96 -103 .

    • Search Google Scholar

  • Girard, P. (1994) β-Carotene producing mutants of Phaffia rhodozyma. Appl. Microbiol. Biotechnol. 41, 183–191.

    Girard P. , 'β-Carotene producing mutants of Phaffia rhodozyma ' (1994 ) 41 Appl. Microbiol. Biotechnol. : 183 -191 .

    • Search Google Scholar

  • Greenberg, J. T. (1990) Positive control of a global antioxidant defense regulon activated by superoxide-generating agents in Escherichia coli. Proc. Natl. Acad. Sci. USA 87, 6181–6185.

    Greenberg J. T. , 'Positive control of a global antioxidant defense regulon activated by superoxide-generating agents in Escherichia coli ' (1990 ) 87 Proc. Natl. Acad. Sci. USA : 6181 -6185 .

    • Search Google Scholar

  • Halliwell, B. (1999) Free Radicals in Biology and Medicine. Oxford University Press, Oxford.

    Halliwell B. , '', in Free Radicals in Biology and Medicine , (1999 ) -.

  • Henderson, L. M. (1993) Dihydrorhodamine 123: a fluorescent probe for superoxide generation? Eur. J. Biochem. 217, 973–980.

    Henderson L. M. , 'Dihydrorhodamine 123: a fluorescent probe for superoxide generation? ' (1993 ) 217 Eur. J. Biochem. : 973 -980 .

    • Search Google Scholar

  • Johnson, E. A. (2003) Phaffia rhodozyma: colorful odyssey. Int. Microbiol. 6, 169–174.

    Johnson E. A. , 'Phaffia rhodozyma: colorful odyssey ' (2003 ) 6 Int. Microbiol. : 169 -174 .

    • Search Google Scholar

  • Kucsera, J. (1998) Homothallic life cycle in the diploid red yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma). Antonie van Leeuwenhock 73, 163–168.

    Kucsera J. , 'Homothallic life cycle in the diploid red yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma) ' (1998 ) 73 Antonie van Leeuwenhock : 163 -168 .

    • Search Google Scholar

  • Kucsera, J. (1998) Life cycle and inheritance of the red yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma). In: Ann. Rev. of Res. Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University 2, 58–62.

    Kucsera J. , 'Life cycle and inheritance of the red yeast Xanthophyllomyces dendrorhous (Phaffia rhodozyma) ' (1998 ) 2 Ann. Rev. of Res. Center for Pathogenic Fungi and Microbial Toxicoses : 58 -62 .

    • Search Google Scholar

  • Lee, J. (1995) Adaptive response of Schizosaccharomyces pombe to hydrogen peroxide and menadione. Micobiol. 141, 3127–3132.

    Lee J. , 'Adaptive response of Schizosaccharomyces pombe to hydrogen peroxide and menadione ' (1995 ) 141 Micobiol. : 3127 -3132 .

    • Search Google Scholar

  • Liu, B. H. (1999) Composition and biosynthetic pathways of carotenoids in the astaxanthin-producing green alga Chlorococcum sp. Bitoech. Lett. 21, 1007–1010.

    Liu B. H. , 'Composition and biosynthetic pathways of carotenoids in the astaxanthin-producing green alga Chlorococcum sp ' (1999 ) 21 Bitoech. Lett. : 1007 -1010 .

    • Search Google Scholar

  • Oberley, L. W. (1984) Assay of superoxide dismutase activity in tumor tissue. Methods Enzymol. 105, 457–464.

    Oberley L. W. , 'Assay of superoxide dismutase activity in tumor tissue ' (1984 ) 105 Methods Enzymol. : 457 -464 .

    • Search Google Scholar

  • Palágyi, Zs. (2004) Genetic variability of astaxanthin-producing yeasts: random amplified polymorphic DNA (RAPD) analysis of Phaffia rhodozyma (Xanthophyllomyces dendrorhous). Acta Biol. Szegediensis 48, 35–38.

    Palágyi Zs. , 'Genetic variability of astaxanthin-producing yeasts: random amplified polymorphic DNA (RAPD) analysis of Phaffia rhodozyma (Xanthophyllomyces dendrorhous) ' (2004 ) 48 Acta Biol. Szegediensis : 35 -38 .

    • Search Google Scholar

  • Pesti, M. (2002) Chromate sensitivity in fission yeast is caused by increased glutathione reductase activity and peroxide overproduction. J. Basic Microbiol. 42, 410–421.

    Pesti M. , 'Chromate sensitivity in fission yeast is caused by increased glutathione reductase activity and peroxide overproduction ' (2002 ) 42 J. Basic Microbiol. : 410 -421 .

    • Search Google Scholar

  • Peterson, G. L. (1983) Determination of total protein. Methods Enzymol. 91, 86–105.

    Peterson G. L. , 'Determination of total protein ' (1983 ) 91 Methods Enzymol. : 86 -105 .

    • Search Google Scholar

  • Pinto, M. C. (1984) Reversible inactivation of Saccharomyces cerevisiae glutathione reductase under reducing conditions. Arch. Biochem. Biophys. 228, 1–12.

    Pinto M. C. , 'Reversible inactivation of Saccharomyces cerevisiae glutathione reductase under reducing conditions ' (1984 ) 228 Arch. Biochem. Biophys. : 1 -12 .

    • Search Google Scholar

  • Rapta, P. (2005) Scavenging and antioxidant properties of compounds synthesized by carotenogenic yeasts stressed by heavy metals — EPR spin trapping study. Biophys. Chem. 116, 1–9.

    Rapta P. , 'Scavenging and antioxidant properties of compounds synthesized by carotenogenic yeasts stressed by heavy metals — EPR spin trapping study ' (2005 ) 116 Biophys. Chem. : 1 -9 .

    • Search Google Scholar

  • Roggenkamp, R. (1974) Microbial assimilation of methanol induction and function of catalase in Candida boidinii. FEBS Lett. 41, 283–286.

    Roggenkamp R. , 'Microbial assimilation of methanol induction and function of catalase in Candida boidinii ' (1974 ) 41 FEBS Lett. : 283 -286 .

    • Search Google Scholar

  • Rush, G. F. (1985) Organic hydroperoxide-induced lipid peroxidation and cell death in isolated hepatocytes. Toxicol. Appl. Pharmacol. 78, 473–483.

    Rush G. F. , 'Organic hydroperoxide-induced lipid peroxidation and cell death in isolated hepatocytes ' (1985 ) 78 Toxicol. Appl. Pharmacol. : 473 -483 .

    • Search Google Scholar

  • Scandalios, J. G. (2005) Oxidative stress: molecular perception and transduction of signals triggering antioxidant gene defenses. Braz. J. Med. Biol. Res. 38, 995–1014.

    Scandalios J. G. , 'Oxidative stress: molecular perception and transduction of signals triggering antioxidant gene defenses ' (2005 ) 38 Braz. J. Med. Biol. Res. : 995 -1014 .

    • Search Google Scholar

  • Schroeder, W. A. (1993) Antioxidant role of carotenoids in Phaffia rhodozyma. J. Gen. Microbiol. 139, 907–912.

    Schroeder W. A. , 'Antioxidant role of carotenoids in Phaffia rhodozyma ' (1993 ) 139 J. Gen. Microbiol. : 907 -912 .

    • Search Google Scholar

  • Shi, X. (1990) On the hydroxyl radical formation in the reaction between hydrogen peroxide and biologically generated chromium(V) species. Arch. Biochem. Biophys. 277, 342–350.

    Shi X. , 'On the hydroxyl radical formation in the reaction between hydrogen peroxide and biologically generated chromium(V) species ' (1990 ) 277 Arch. Biochem. Biophys. : 342 -350 .

    • Search Google Scholar

  • Stacey, N. H. (1986) The amelioration of cadmium-induced injury in isolated hepatocytes by reduced glutathione. Toxicol. 42, 85–93.

    Stacey N. H. , 'The amelioration of cadmium-induced injury in isolated hepatocytes by reduced glutathione ' (1986 ) 42 Toxicol. : 85 -93 .

    • Search Google Scholar

  • Subczynski, W. K. (2000) Physical properties of lipid bilayer membranes: relevance to membrane biological functions. Acta Biochim. Pol. 47, 613–623.

    Subczynski W. K. , 'Physical properties of lipid bilayer membranes: relevance to membrane biological functions ' (2000 ) 47 Acta Biochim. Pol. : 613 -623 .

    • Search Google Scholar

  • Visser, H. (2003) Metabolic engineering of the astaxanthin-biosynthetic pathway of Xanthophyllomyces dendrorhous. FEMS Yeast Res. 4, 221–231.

    Visser H. , 'Metabolic engineering of the astaxanthin-biosynthetic pathway of Xanthophyllomyces dendrorhous ' (2003 ) 4 FEMS Yeast Res. : 221 -231 .

    • Search Google Scholar

  • Wang, W. (1998) Endogenous glutathione conjugates: occurrence and biological functions. Pharmacol. Rev. 50, 335–356.

    Wang W. , 'Endogenous glutathione conjugates: occurrence and biological functions ' (1998 ) 50 Pharmacol. Rev. : 335 -356 .

    • Search Google Scholar

  • Wilkinson, M. G. (1996) The Atfl transcription factor is a target for the Styl stress-activated MAP kinase pathway in fission yeast. Genes Dev. 10, 2289–2301.

    Wilkinson M. G. , 'The Atfl transcription factor is a target for the Styl stress-activated MAP kinase pathway in fission yeast ' (1996 ) 10 Genes Dev. : 2289 -2301 .

    • Search Google Scholar
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Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X

Senior editors

Editor(s)-in-Chief: Elekes, Károly

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

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Acta Biologica Hungarica
Language English
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Year of
Foundation		 1950
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Programme		 changed title
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Founder Magyar Tudományos Akadémia
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Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
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ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)

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