Acta Biologica Hungarica
Volume/Issue:
Volume 62: Issue 3
Evaluation of roundup-induced toxicity on genetic material and on length growth of barley seedlings
Authors:
Elena Truta Biological Research Institute Lascar Catargi Street 47 700107 Iasi Romania

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Gabriela Vochita Biological Research Institute Lascar Catargi Street 47 700107 Iasi Romania

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Craita Rosu Biological Research Institute Lascar Catargi Street 47 700107 Iasi Romania

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Maria-Magdalena Zamfirache University “Al. I. Cuza” Faculty of Biology Carol I 20A Bd. 700506 Iasi Romania

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Zenovia Olteanu University “Al. I. Cuza” Faculty of Biology Carol I 20A Bd. 700506 Iasi Romania

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Pages:
290–301
Online Publication Date:
13 Aug 2011
Publication Date:
01 Sep 2011
Article Category:
Research Article
DOI:
https://doi.org/10.1556/abiol.62.2011.3.8
Keywords:
Barley; chromosome aberrations; genotoxic effects; glyphosate; Roundup
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The study was performed in order to evaluate Roundup-induced genotoxic effects in Hordeum vulgare L. cv. Madalin root meristems and to analyze herbicide impact on length growth of barley seedlings. Caryopses were treated for 3 hours and 6 hours with 0.1%, 0.5%, 1.0% and 2.0% Roundup solutions (v/v), containing 0.36 mg ml−1, 1.8 mg ml−1, 3.6 mg ml−1 and 7.2 mg ml−1 glyphosate active ingredient. Mitotic index decreased in both exposure times with concentration increase. In 3-h treatment, its average values decreased from 4.73 ± 0.31% to 1.51 ± 0.43%, whereas in 6-h treatment this parameter declined from 3.86 ± 0.92% to 0.62 ± 0.15%. The highest ana-telophase aberration rates were noted in 3-h treatments (8.91%, 9.19%, 9.47%, 11.25%, comparatively to control — 5.99%). Roundup enhanced the number of metaphase disturbances proving its noxious effect on normal functioning of mitotic spindle. Seedling growth was negatively influenced at all tested concentrations in both exposure times. The length decreased as concentration increased, so that the average length is 7.5–9 times smaller than in control at the maximum concentration, in both exposures.

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Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X

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Editor(s)-in-Chief: Elekes, Károly

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    1. Csányi, Vilmos (Göd)
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    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

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Acta Biologica Hungarica
Language English
Size  
Year of
Foundation		 1950
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Programme		 changed title
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Founder Magyar Tudományos Akadémia
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Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
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ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)

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