View More View Less
  • 1 Acharya & B.M. Reddy College of Pharmacy Department of Pharmacology Bangalore 560 090 Karnataka India
  • | 2 Indian Institute of Science Department of Biochemistry Bangalore 560 012 Karnataka India
  • | 3 Chalapathi Institute of Pharmaceutical Science Department of Pharmaceutical Chemistry Guntur 522 034 Andhra Pradesh India
Restricted access

The present study was to investigate the effect of W. calendulacea on ischemia and reperfusion-induced cerebral injury. Cerebral ischemia was induced by occluding right and left common carotid arteries (global cerebral ischemia) for 30 min followed by reperfusion for 1 h and 4 h individually. Various biochemical alterations, produced subsequent to the application of bilateral carotid artery occlusion (BCAO) followed by reperfusion viz. increase in lipid peroxidation (LPO), hydrogen peroxide (H2O2), and decrease in reduced glutathione (GSH), catalase (CAT) and superoxide dismutase (SOD), level in the brain tissue, Western blot analysis (Cu-Zn-SOD and CAT) and assessment of cerebral infarct size were measured. All those enzymes are markedly reversed and restored to near normal level in the groups pretreated with W. calendulacea (250 and 500 mg/kg given orally in single and double dose/day for 10 days) in dose-dependent way. The effect of W. calendulacea had increased significantly the protein expression of copper/zinc superoxide dismutase (Cu-Zn-SOD) and CAT in cerebral ischemia. W. claendulacea was markedly decrease cerebral infarct damages but results are not statistically significant. It can be concluded that W. calendulacea possesses a neuroprotective activity against cerebral ischemia in rat.

  • Bederson, J. B., Pitts, L. H., Germano, S. M., Nishimura, M. C., Davis, R. L., Bartkowski, H. M. (1986) Evaluation of 2,3,5-triphenyltetrazolium chloride as a stain for detection and quantification of experimental cerebral infarction in rats. Stroke 17, 1304–1308.

    Bartkowski H. M. , 'Evaluation of 2,3,5-triphenyltetrazolium chloride as a stain for detection and quantification of experimental cerebral infarction in rats ' (1986 ) 17 Stroke : 1304 -1308.

    • Search Google Scholar
  • Chan, P. H. (2001) Reactive oxygen radicals in signaling and damage in the ischemic brain. J. Cereb. Blood Metab. 21, 2–14.

    Chan P. H. , 'Reactive oxygen radicals in signaling and damage in the ischemic brain ' (2001 ) 21 J. Cereb. Blood Metab. : 2 -14.

    • Search Google Scholar
  • Claiborne, A. (1985) Catalase activity, In: Greenwald, R. A. (ed.), Hand Book of Methods for Oxygen Radical Research. CRC Press, Boca Raton, Florida, USA, pp. 283–284.

    Claiborne A. , '', in Hand Book of Methods for Oxygen Radical Research , (1985 ) -.

  • Cui, Ke., Luo, X., Xu, K., Murthy, M. R. V. (2004) Role of oxidative stress in neurodegeneration: recent developments in assay methods for oxidative stress and nutraceutical antioxidants. Prog. Neuropsychopharmacol. Biol. Psychiatry 28, 771–799.

    Murthy M. R. V. , 'Role of oxidative stress in neurodegeneration: recent developments in assay methods for oxidative stress and nutraceutical antioxidants ' (2004 ) 28 Prog. Neuropsychopharmacol. Biol. Psychiatry : 771 -799.

    • Search Google Scholar
  • Cuzzocrea, S., McDonald, M. C., Mazzon, E., Siriwardena, D., Costantino, G., Fulia, F., Cucinotta, G., Gitto, E., Cordaro, S., Barberi, I., De Sarro, A., Caputi, A. P., Thiemermann, C. (2000) Effects of tempol, a membrane-permeable radical scavenger, in a gerbil model of brain injury. Brain Res. 875, 96–106.

    Thiemermann C. , 'Effects of tempol, a membrane-permeable radical scavenger, in a gerbil model of brain injury ' (2000 ) 875 Brain Res. : 96 -106.

    • Search Google Scholar
  • Dhindsa, R. S., Plumb-Dhindsa, P., Thorpe, T. A. (1981) Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. J. Exp. Botany 32, 93–101.

    Thorpe T. A. , 'Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase ' (1981 ) 32 J. Exp. Botany : 93 -101.

    • Search Google Scholar
  • Dringen, R. (2000) Metabolism and functions of glutathione in brain. Prog. Neurobiol. 62, 649–671.

    Dringen R. , 'Metabolism and functions of glutathione in brain ' (2000 ) 62 Prog. Neurobiol. : 649 -671.

    • Search Google Scholar
  • Fridovich, I. (1975) Superoxide dismutases. Annu. Rev. Biochem. 44, 147–159.

    Fridovich I. , 'Superoxide dismutases ' (1975 ) 44 Annu. Rev. Biochem. : 147 -159.

  • Gaspar, T., Domoki, F., Lenti, L., Institoris, A., Snipes, J. A., Bari, F., Busija, D. W. (2009) Neuroprotective effect of adenoviral catalase gene transfer in cortical neuronal cultures. Brain Res. 270, 1–9.

    Busija D. W. , 'Neuroprotective effect of adenoviral catalase gene transfer in cortical neuronal cultures ' (2009 ) 270 Brain Res. : 1 -9.

    • Search Google Scholar
  • Gutteridge, J. M. C., Halliwell, B. (1994) Antioxidants in Nutrition, Health and Disease. Oxford University Press.

  • Govindachari, T. R., Premila, M. S. (1985) The benzofuran norwedelic acid from Wedelia calendulacea. Phytochemistry 24, 3068.

    Premila M. S. , 'The benzofuran norwedelic acid from Wedelia calendulacea ' (1985 ) 24 Phytochemistry : 3068 -.

    • Search Google Scholar
  • Harlow, L., Lane, D. (1988) Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA.

    Lane D. , '', in Antibodies: A Laboratory Manual , (1988 ) -.

  • Imaizumi, S., Tominaga, T., Uenohara, H., Yoshimoto, T., Suzuki, J., Fujita, Y. (1986) Initiation and propagation of lipid peroxidation in cerebral infarction models. Experimental studies. Neurol. Res. 8, 214–220.

    Fujita Y. , 'Initiation and propagation of lipid peroxidation in cerebral infarction models. Experimental studies ' (1986 ) 8 Neurol. Res. : 214 -220.

    • Search Google Scholar
  • Imaizumi, S., Kayama, T., Suzuki, J. (1984) Chemiluminescence in hypoxic brain-the first report. Correlation between energy metabolism and free radical reaction. Stroke 15, 1061–1065.

    Suzuki J. , 'Chemiluminescence in hypoxic brain-the first report. Correlation between energy metabolism and free radical reaction ' (1984 ) 15 Stroke : 1061 -1065.

    • Search Google Scholar
  • Jollow, D. J., Mitchell, J. R., Zampaghone, N., Gillete, J. R. (1974) Bromobenzene induced oxide as the hepatotoxic intermediate. Pharmacology 11, 161–169.

    Gillete J. R. , 'Bromobenzene induced oxide as the hepatotoxic intermediate ' (1974 ) 11 Pharmacology : 161 -169.

    • Search Google Scholar
  • Kelsey, N. A., Wilkins, H. M., Linseman, D. A. (2010) Nutraceutical antioxidants as novel neuroprotective agents. Molecules 15, 7792–7814.

    Linseman D. A. , 'Nutraceutical antioxidants as novel neuroprotective agents ' (2010 ) 15 Molecules : 7792 -7814.

    • Search Google Scholar
  • Kim, Y. C. (2010) Neuroprotective phenolics in medicinal plants. Arch. Pharm. Res. 33, 1611–1632.

    Kim Y. C. , 'Neuroprotective phenolics in medicinal plants ' (2010 ) 33 Arch. Pharm. Res. : 1611 -1632.

    • Search Google Scholar
  • Kobori, M., Yang, Z., Gong, D., Heissmeyer, V., Zhu, H., Jung, Y.-K., Angelica, M., Gakidis, M., Rao, A., Sekine, T., Ikegami, F., Yuan, C., Yuan, J. (2004) Wedelolactone suppresses LPS-induced caspase-11 expression by directly inhibiting the IKK Complex. Cell Death Differ. 11, 123–130.

    Yuan J. , 'Wedelolactone suppresses LPS-induced caspase-11 expression by directly inhibiting the IKK Complex ' (2004 ) 11 Cell Death Differ. : 123 -130.

    • Search Google Scholar
  • Lapchak, P. A., Araujo, D. M. (2007) Advances in ischemic stroke treatment: neuroprotective and combination therapies. Expert. Opin. Emerg. Drugs 12, 97–112.

    Araujo D. M. , 'Advances in ischemic stroke treatment: neuroprotective and combination therapies ' (2007 ) 12 Expert. Opin. Emerg. Drugs : 97 -112.

    • Search Google Scholar
  • Lowry, O. H., Rosebrough, N. J., Fair, A. L., Randall, R. J. (1951) Protein measurement with Folin phenol reagent. J. Biol. Chem. 193, 265–275.

    Randall R. J. , 'Protein measurement with Folin phenol reagent ' (1951 ) 193 J. Biol. Chem. : 265 -275.

    • Search Google Scholar
  • Murakami, K., Kondo, T., Kawase, M., Li, Y., Sato, S., Chen, S. F. (1998) Mitochondrial susceptibility to oxidative stress exacerbates cerebral infarction that follows permanent focal cerebral ischemia in mutant mice with manganese superoxide dismutase deficiency. J. Neurosci. 18, 205–213.

    Chen S. F. , 'Mitochondrial susceptibility to oxidative stress exacerbates cerebral infarction that follows permanent focal cerebral ischemia in mutant mice with manganese superoxide dismutase deficiency ' (1998 ) 18 J. Neurosci. : 205 -213.

    • Search Google Scholar
  • Nadkarni, A. K. (1976) Indian Materia medica. I. India Popular Prakashan Pvt. Ltd., Bombay.

    Nadkarni A. K. , '', in Indian Materia medica , (1976 ) -.

  • Oliver, C. N., Starke-Reed, P. E., Stadtman, E. R., Liu, G. J., Carney, J. M., Floyd, R. A. (1990) Oxidative damage to brain proteins, loss of glutamine synthetase activity, and production of free radicals during ischemia/reperfusion-induced injury to gerbil brain. Proc. Natl. Acad. Sci. U. S. A. 87, 5144–5147.

    Floyd R. A. , 'Oxidative damage to brain proteins, loss of glutamine synthetase activity, and production of free radicals during ischemia/reperfusion-induced injury to gerbil brain ' (1990 ) 87 Proc. Natl. Acad. Sci. U. S. A. : 5144 -5147.

    • Search Google Scholar
  • Prakash, T., Rama Rao, N., Viswanatha Swamy, A. H. M. (2008) Neuropharmacological studies on Wedelia calendulacea Less stem extract. Phytomedicine 15, 959–970.

    Viswanatha Swamy A. H. M. , 'Neuropharmacological studies on Wedelia calendulacea Less stem extract ' (2008 ) 15 Phytomedicine : 959 -970.

    • Search Google Scholar
  • Ravindranath, V., Reed, D. J. (1990) Glutathione depletion and formation of glutathione-protein mixed disulphide following exposure of brain mitochondria to oxidative stress. Biochem. Biophys. Res. Commun. 169, 1075–1079.

    Reed D. J. , 'Glutathione depletion and formation of glutathione-protein mixed disulphide following exposure of brain mitochondria to oxidative stress ' (1990 ) 169 Biochem. Biophys. Res. Commun. : 1075 -1079.

    • Search Google Scholar
  • Sakamoto, A., Ohnishi, S. T., Ohnishi, T., Ogawa, R. (1991) Relationship between free radical production and lipid peroxidation during ischemia-reperfusion injury in the rat brain. Brain Res. 554, 186–192.

    Ogawa R. , 'Relationship between free radical production and lipid peroxidation during ischemia-reperfusion injury in the rat brain ' (1991 ) 554 Brain Res. : 186 -192.

    • Search Google Scholar
  • Schulz, J. B., Lindenau, J., Seyfried, J., Dichgans, J. (2000) Glutathione, oxidative stress and neurodegeneration. Eur. J. Biochem. 267, 4904–4911.

    Dichgans J. , 'Glutathione, oxidative stress and neurodegeneration ' (2000 ) 267 Eur. J. Biochem. : 4904 -4911.

    • Search Google Scholar
  • Sims, N. R., Anderson, M. F., Hobbs, L. M., Kong, J. Y., Phillips, S., Powell, J. A., Zaidan, E. (2000) Impairment of brain mitochondrial function by hydrogen peroxide. Mol. Brain Res. 77, 176–184.

    Zaidan E. , 'Impairment of brain mitochondrial function by hydrogen peroxide ' (2000 ) 77 Mol. Brain Res. : 176 -184.

    • Search Google Scholar
  • Utley, H. C., Bernhein, F., Hochslein, P. (1967) Effects of sulfhydryl reagent on peroxidation in microsomes. Arch. Biochem. Biophys. 260, 521–531.

    Hochslein P. , 'Effects of sulfhydryl reagent on peroxidation in microsomes ' (1967 ) 260 Arch. Biochem. Biophys. : 521 -531.

    • Search Google Scholar
  • Velikova, V., Yordanov, I., Edreva, A. (2000) Oxidative stress and some antioxidant systems in acid rain treated bean plants protective role of exognous polyamines. Plant Sci. 151, 59–66.

    Edreva A. , 'Oxidative stress and some antioxidant systems in acid rain treated bean plants protective role of exognous polyamines ' (2000 ) 151 Plant Sci. : 59 -66.

    • Search Google Scholar
  • Wagner, H., Geyer, B., Kiso, Y., Hikino, H., Rao, G. S. (1986) Coumestans as main active principles of the liver drugs EcliPta alba and Wedelia calendulaceae. Planta Medica 52, 370–374.

    Rao G. S. , 'Coumestans as main active principles of the liver drugs EcliPta alba and Wedelia calendulaceae ' (1986 ) 52 Planta Medica : 370 -374.

    • Search Google Scholar
  • Zahoor Ahmad Shah, Rabia Afzal Gilani, Pragya Sharma, Shashi Bharat Vohora (2005) Cerebroprotective effect of Korean ginseng tea against global and focal models of ischemia in rats. J. Ethnopharmacol. 101, 299–307.

    Vohora S. B. , 'Cerebroprotective effect of Korean ginseng tea against global and focal models of ischemia in rats ' (2005 ) 101 J. Ethnopharmacol. : 299 -307.

    • Search Google Scholar

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

Acta Biologica Hungarica
P.O. Box 35
H-8237 Tihany, Hungary
Phone: (36 87) 448 244 ext. 103
Fax: (36 87) 448 006
E-mail: elekes@tres.blki.hu

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture
  • Biology and Environmental Sciences
  • Elsevier BIOBASE
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Acta Biologica Hungarica
Language English
Size  
Year of
Foundation
1950
Publication
Programme
changed title
Volumes
per Year
 
Issues
per Year
 
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)