Acta Biologica Hungarica
Volume/Issue:
Volume 62: Issue 4
Efficient In vitro plant regeneration through leaf base derived callus cultures of abiotic stress sensitive popular Asian Indica rice cultivar IR 64 (Oryza sativa L.)
Authors:
A. Mohana Priya Alagappa University Department of Biotechnology Karaikudi 630 003 Tamil Nadu India

Search for other papers by A. Mohana Priya in
Current site
Google Scholar
PubMed Close
,
S. Karutha Pandian Alagappa University Department of Biotechnology Karaikudi 630 003 Tamil Nadu India

Search for other papers by S. Karutha Pandian in
Current site
Google Scholar
PubMed Close
, and
M. Ramesh Alagappa University Department of Biotechnology Karaikudi 630 003 Tamil Nadu India

Search for other papers by M. Ramesh in
Current site
Google Scholar
PubMed Close
Pages:
441–452
Online Publication Date:
26 Nov 2011
Publication Date:
01 Dec 2011
Article Category:
Research Article
DOI:
https://doi.org/10.1556/abiol.62.2011.4.9
Keywords:
2,4-D; embryogenic callus; in vitro ; leaf base; Oryza sativa ; regeneration
Restricted access

A simple and efficient protocol has been developed for high frequency plant regeneration through callus cultures derived from leaf bases of abiotic stress sensitive Asian indica rice variety IR 64. Leaf base segments (4–5 mm diameter) were obtained from 6-day-old dark grown seedlings germinated on halfstrength Murashige and Skoog medium and cultured on MS medium supplemented with different concentrations of 2,4-Dichlorophenoxyacetic acid (2.2–18 μM) and Kinetin (0.2–1.7 μM). Among the various combinations, 13.5 μM 2,4-D and 1.3 μM Kn resulted in high callus induction frequency (87.5%) with a maximum fresh weight of 0.22 g per segment. The regeneration frequency was 75.5% with multiple shoots within 3 weeks of transfer on MS medium supplemented with 13.3 μM 6-benzylamino purine and 8 μM Naphthaleneacetic acid. The shoots readily rooted on half-strength MS medium without any hormonal supplements. In vitro regenerated plantlets with multiple shoots and roots were transferred to sterile soil and vermiculite mix and maintained in shade house for 30 days. Complete plantlets were then transferred to nursery and acclimatized to the external environment until seed set. RAPD profile reveals monomorphism and thus confirming the genetic stability of the regenerated plants. This method has the potential for both direct as well as indirect method of transformation for the production of genetically modified plants.

  • Arockiasamy, S., Rani, S. S., Ignacimuthu, S., Melchias, G. (2006) Efficient protocols for in vitro regeneration of Pennisetum glaucum (L) Br. Indian J. Exp. Biol. 44, 757–761.

    Melchias G. , 'Efficient protocols for in vitro regeneration of Pennisetum glaucum (L) Br ' (2006 ) 44 Indian J. Exp. Biol. : 757 -761 .

    • Search Google Scholar

  • Bhaskaran, S., Smith, R. H. (1990) Regeneration in cereal tissue culture: A review. Crop Sci. 30, 1328–1336.

    Smith R. H. , 'Regeneration in cereal tissue culture: A review ' (1990 ) 30 Crop Sci. : 1328 -1336 .

    • Search Google Scholar

  • Biswas, A., Mandal, A. B. (2007) Plant regeneration in different genotypes of indica rice. Indian J. Biotech. 6, 532–540.

    Mandal A. B. , 'Plant regeneration in different genotypes of indica rice ' (2007 ) 6 Indian J. Biotech. : 532 -540 .

    • Search Google Scholar

  • Boissot, N., Valdez, M., Guiderdoni, E. (1990) Plant regeneration from leaf and seed-derived calli and suspension cultures of the African perennial wild rice, Oryza longistaminata. Plant Cell Rep. 9, 447–450.

    Guiderdoni E. , 'Plant regeneration from leaf and seed-derived calli and suspension cultures of the African perennial wild rice, Oryza longistaminata ' (1990 ) 9 Plant Cell Rep. : 447 -450 .

    • Search Google Scholar

  • Castillo, A. M., Egana, B., Sanz, J. M., Cistue, L. (1998) Somatic embryogenesis and plant regeneration from barley cultivars grown in Spain. Plant Cell Rep. 17, 902–906.

    Cistue L. , 'Somatic embryogenesis and plant regeneration from barley cultivars grown in Spain ' (1998 ) 17 Plant Cell Rep : 902 -906 .

    • Search Google Scholar

  • Chen, Z., Zhuge, Q., Sundqvist, C. (1995) Oat leaf base: tissue with an efficient regeneration capacity. Plant Cell Rep. 14, 354–358.

    Sundqvist C. , 'Oat leaf base: tissue with an efficient regeneration capacity ' (1995 ) 14 Plant Cell Rep : 354 -358 .

    • Search Google Scholar

  • Chuanyin, W., Zapata, F. J. (1992) Plant regeneration from protoplasts isolated from primary callus of four japonica rice (Oryza sativa L.) varieties. Plant Sci. 86, 83–87.

    Zapata F. J. , 'Plant regeneration from protoplasts isolated from primary callus of four japonica rice (Oryza sativa L.) varieties ' (1992 ) 86 Plant Sci : 83 -87 .

    • Search Google Scholar

  • Datta, K., Potrykus, Datta, S. K. (1996) Efficient fertile plant regeneration from protoplasts of indica rice breeding line IR 72 (Oryza sativa L.). Plant Cell Rep. 11, 229–233.

    Datta S. K. , 'Efficient fertile plant regeneration from protoplasts of indica rice breeding line IR 72 (Oryza sativa L.) ' (1996 ) 11 Plant Cell Rep : 229 -233 .

    • Search Google Scholar

  • Feldmann, P., Sapotille, J., Gredoire, P., Rott, P. (1994) Micropropagation of sugarcane. In: Teisson, C. (ed.) In vitro culture of tropical plants. CIRAD, Montpellier, France, pp. 15–17.

    Rott P. , '', in In vitro culture of tropical plants , (1994 ) -.

  • Furuhashi, K., Yatazuva, M. (1964) Indefinitely growing rice callus in tissue culture. Kagaku. 34, 623.

    Yatazuva M. , 'Indefinitely growing rice callus in tissue culture ' (1964 ) 34 Kagaku : 623 -.

    • Search Google Scholar

  • Gamborg, O. L., Miller, R. A., Ojima, K. (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell. Res. 50, 151–158.

    Ojima K. , 'Nutrient requirements of suspension cultures of soybean root cells ' (1968 ) 50 Exp. Cell. Res. : 151 -158 .

    • Search Google Scholar

  • Genovesi, A. D., Magil, C. W. (1982) Embryogenesis in callus derived from rice microspores. Plant Cell Rep. 1, 257–260.

    Magil C. W. , 'Embryogenesis in callus derived from rice microspores ' (1982 ) 1 Plant Cell Rep : 257 -260 .

    • Search Google Scholar

  • Guha-Mukerjee, S. (1973) Genotypic differences in the in vitro formation of embryoids from rice pollen. J. Exp. Bot. 24, 139–144.

    Guha-Mukerjee S. , 'Genotypic differences in the in vitro formation of embryoids from rice pollen ' (1973 ) 24 J. Exp. Bot. : 139 -144 .

    • Search Google Scholar

  • Haliloglu, K. (2006) Efficient regeneration system from wheat leaf base segments. Biol. Plant. 50, 326–330.

    Haliloglu K. , 'Efficient regeneration system from wheat leaf base segments ' (2006 ) 50 Biol. Plant. : 326 -330 .

    • Search Google Scholar

  • Ikram ul Haq, Xing, Z. C., Mukhtar, Z., Jaleel, C. A., Azooz, M. M. (2009) Effect of physical desiccation on plant regeneration efficiency in rice (Oryza sativa L.) variety super basmati. J. Plant Physiol. 166, 1568–1575.

    Azooz M. M. , 'Effect of physical desiccation on plant regeneration efficiency in rice (Oryza sativa L.) variety super basmati ' (2009 ) 166 J. Plant Physiol. : 1568 -1575 .

    • Search Google Scholar

  • Kabir, A. H., Mahfuz, I., Razvy, M. A., Ahmed, M. B., Alam, M. F. (2008) Indirect organogenesis and somaclonal variation in four rice cultivars of Bangladesh. J. Appl. Sci. Res. 4, 451–458.

    Alam M. F. , 'Indirect organogenesis and somaclonal variation in four rice cultivars of Bangladesh ' (2008 ) 4 J. Appl. Sci. Res. : 451 -458 .

    • Search Google Scholar

  • Keresa, S., Mihovilovic, A., Perica, M. C., Mitic, B., Baric, M., Vesek, I., Marchetti, S. (2009) In vitro regeneration of the croatioan endemic species Iris adriatica trinajstic ex metic. Acta Biol. Cracov Bot. 51, 7–12.

    Marchetti S. , 'In vitro regeneration of the croatioan endemic species Iris adriatica trinajstic ex metic ' (2009 ) 51 Acta Biol. Cracov Bot. : 7 -12 .

    • Search Google Scholar

  • Khush, G. S. (1997) Origin, dispersal, cultivation and variation of rice. Plant Mol. Biol. 35, 25–34.

    Khush G. S. , 'Origin, dispersal, cultivation and variation of rice ' (1997 ) 35 Plant Mol. Biol. : 25 -34 .

    • Search Google Scholar

  • Khush, G. S. (2005) What it will take to feed 5.0 billion rice consumers in 2030? Plant Mol. Biol. 59, 1–6.

    Khush G. S. , 'What it will take to feed 5.0 billion rice consumers in 2030? ' (2005 ) 59 Plant Mol. Biol. : 1 -6 .

    • Search Google Scholar

  • Kumar, K. K., Maruthasalam, S., Loganathan, M., Sudhakar, D., Balasubramanian, P. (2005) An improved Agrobacterium-mediated transformation protocol for recalcitrant elite indica rice cultivars. Plant Mol. Biol. Rep. 23, 67–73.

    Balasubramanian P. , 'An improved Agrobacterium-mediated transformation protocol for recalcitrant elite indica rice cultivars ' (2005 ) 23 Plant Mol. Biol. Rep. : 67 -73 .

    • Search Google Scholar

  • Lal, N., Singh, H. N. (1994) Rapid clonal multiplication of sugarcane through tissue culture. Plant Tissue Cult. 4, 1–7.

    Singh H. N. , 'Rapid clonal multiplication of sugarcane through tissue culture ' (1994 ) 4 Plant Tissue Cult : 1 -7 .

    • Search Google Scholar

  • Mikami, T., Kinoshita, T. (1988) Genotypic effects on callus formation from different explants of rice, Oryza sativa. Plant Cell Tissue Organ Cult. 12, 311–314.

    Kinoshita T. , 'Genotypic effects on callus formation from different explants of rice, Oryza sativa ' (1988 ) 12 Plant Cell Tissue Organ Cult : 311 -314 .

    • Search Google Scholar

  • Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497.

    Skoog F. , 'A revised medium for rapid growth and bioassays with tobacco tissue cultures ' (1962 ) 15 Physiol. Plant : 473 -497 .

    • Search Google Scholar

  • Oinam, G. S., Kothari, S. L. (1995) Totipotency of coleoptile tissue in indica rice (Oryza sativa L. cv. CH1039). Plant Cell Rep. 14, 245–248.

    Kothari S. L. , 'Totipotency of coleoptile tissue in indica rice (Oryza sativa L. cv. CH1039) ' (1995 ) 14 Plant Cell Rep : 245 -248 .

    • Search Google Scholar

  • Othmani, A., Rhouman, S., Bayoudh, C., Mzid, R., Drira, N., Trifi, M. (2010) Regeneration and analysis of genetic stability of plantlets as revealed by RAPD and AFLP markers in date palm (Phoenix dactylifera L.) cv. Deglet Nour. Int. Res. j. plant sci. 3, 48–55.

    Trifi M. , 'Regeneration and analysis of genetic stability of plantlets as revealed by RAPD and AFLP markers in date palm (Phoenix dactylifera L.) cv. Deglet Nour ' (2010 ) 3 Int. Res. j. plant sci. : 48 -55 .

    • Search Google Scholar

  • Panda, M. K., Mohanty, S., Subudhi, E., Acharya, L., Nayak, S. (2007) Assessment of genetic stability of micropropagated plants of Curcuma longa L. by cytophotometry and RAPD analyses. Int. j. Integr. Biol. 1, 189–195.

    Nayak S. , 'Assessment of genetic stability of micropropagated plants of Curcuma longa L. by cytophotometry and RAPD analyses ' (2007 ) 1 Int. j. Integr. Biol. : 189 -195 .

    • Search Google Scholar

  • Peng, H., Kononowicz, H., Hodges, T. K. (1992) Transgenic indica rice plants. Theor. Appl. Genet. 83, 855–863.

    Hodges T. K. , 'Transgenic indica rice plants ' (1992 ) 83 Theor. Appl. Genet. : 855 -863 .

    • Search Google Scholar

  • Rajyalakshmi, K., Grover, A., Maheswari, N., Tyagi, A. K., Maheswari, S. C. (1991) High frequency regeneration of plantlets from the leaf-bases via somatic embryogenesis and comparison of polypeptide profiles from morphogenic and non-morphogenic calli in wheat. Physiol. Plant. 82, 517–523.

    Maheswari S. C. , 'High frequency regeneration of plantlets from the leaf-bases via somatic embryogenesis and comparison of polypeptide profiles from morphogenic and non-morphogenic calli in wheat ' (1991 ) 82 Physiol. Plant : 517 -523 .

    • Search Google Scholar

  • Ramesh, M., Murugiah, V., Aditya Gupta, K. (2009) Efficient in vitro plant regeneration via leaf base segments indica rice (Oryza sativa L.). Indian J. Exp. Biol. 47, 68–74.

    Aditya Gupta K. , 'Efficient in vitro plant regeneration via leaf base segments indica rice (Oryza sativa L.) ' (2009 ) 47 Indian J. Exp. Biol. : 68 -74 .

    • Search Google Scholar

  • Reynoired, J. P., Chriqui, D., Noin, M., Brown, S., Marie, D. (1993) Plant regeneration from in vitro leaf culture of several gerbera species. Plant Cell Tissue Organ Cult. 33, 203–210.

    Marie D. , 'Plant regeneration from in vitro leaf culture of several gerbera species ' (1993 ) 33 Plant Cell Tissue Organ Cult : 203 -210 .

    • Search Google Scholar

  • Roy, B., Mandal, A. B. (2011) Profuse microtillering of androgenic plantlets of elite indica rice variety IR 72. Asian J. Biotechnol. 3, 165–176.

    Mandal A. B. , 'Profuse microtillering of androgenic plantlets of elite indica rice variety IR 72 ' (2011 ) 3 Asian J. Biotechnol. : 165 -176 .

    • Search Google Scholar

  • Sahrawat, A. K., Chand, S. (2001) High-frequency plant regeneration from coleoptile tissue of indica rice (Oryza sativa L.) In Vitro Cell. Dev. Biol.-Plant. 37, 55–61.

    Chand S. , 'High-frequency plant regeneration from coleoptile tissue of indica rice (Oryza sativa L.) In Vitro Cell ' (2001 ) 37 Dev. Biol.-Plant. : 55 -61 .

    • Search Google Scholar

  • Sengupta, S., Majumder, A. L. (2009) Insight into the salt tolerance factors of a wild halophytic rice, Porteresia coarctata: a physiological and proteomic approach. Planta 4, 911–929.

    Majumder A. L. , 'Insight into the salt tolerance factors of a wild halophytic rice, Porteresia coarctata: a physiological and proteomic approach ' (2009 ) 4 Planta : 911 -929 .

    • Search Google Scholar

  • Shannon, M. C. (1998) Adaptation of plants to salinity. Adv. Agron. 60, 75–120.

    Shannon M. C. , 'Adaptation of plants to salinity ' (1998 ) 60 Adv. Agron. : 75 -120 .

  • Shirin, F. M., Hossain, M. F., Kabir, M., Roy., Sarker, S. R. (2007) Callus induction and plant regeneration from intermodal and leaf explants of four potato (Solanum tuberosum L.) cultivars. World J. Agric. Sci. 3, 1–6.

    Sarker S. R. , 'Callus induction and plant regeneration from intermodal and leaf explants of four potato (Solanum tuberosum L.) cultivars ' (2007 ) 3 World J. Agric. Sci. : 1 -6 .

    • Search Google Scholar

  • Skoog, F., Miller, E. O. (1957) Chemical regulation of growth and organ formation in plant tissues cultured in vitro. J. Exp. Biol. 11, 118–131.

    Miller E. O. , 'Chemical regulation of growth and organ formation in plant tissues cultured in vitro ' (1957 ) 11 J. Exp. Biol. : 118 -131 .

    • Search Google Scholar

  • Tyagi, A. K., Kharuna, J. P., Paramjit, K., Raghuvanshi, S., Gaur, A., Kapur, A., Gupta, V., Kumar, D., Ravi, V., Vij, S., Khurana, P., Sharma, S. (2004) Structural and functional analysis of rice genome. J. Genetics 83, 79–99.

    Sharma S. , 'Structural and functional analysis of rice genome ' (2004 ) 83 J. Genetics : 79 -99 .

    • Search Google Scholar

  • Wernicke, W., Brettele, R. (1980) Somatic embryogenesis from Sorghum bicolor leaves. Nature 287, 138–139.

    Brettele R. , 'Somatic embryogenesis from Sorghum bicolor leaves ' (1980 ) 287 Nature : 138 -139 .

    • Search Google Scholar

  • Wernicke, W., Brettell, R. I. S. (1982) Morphogenesis from cultured leaf tissue of Sorghum bicolour-culture initiation. Protoplasma 111, 19–27.

    Brettell R. I. S. , 'Morphogenesis from cultured leaf tissue of Sorghum bicolour-culture initiation ' (1982 ) 111 Protoplasma : 19 -27 .

    • Search Google Scholar

  • Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalski, J. A., Tingey, S. V (1990) DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res. 18, 6531–6535.

    Tingey S. V. , 'DNA polymorphism amplified by arbitrary primers are useful as genetic markers ' (1990 ) 18 Nucl. Acids Res. : 6531 -6535 .

    • Search Google Scholar

  • Wu, J. L., Wu, C., Lei, C., Baraoidan, M., Bordeos, A., Madamba, M. R. S., Ramos-Pamplona, M., Mauleon, R., Portugal, A., Ulat, V. J., Bruskiewich, R., Wang, G., Leach, J., Khush, G., Leung, H. (2005) Chemical and irradiation-induced mutants of indica rice IR64 for forward and reverse genetics. Plant Mol. Biol. 59, 85–97.

    Leung H. , 'Chemical and irradiation-induced mutants of indica rice IR64 for forward and reverse genetics ' (2005 ) 59 Plant Mol. Biol. : 85 -97 .

    • Search Google Scholar
  • Collapse
  • Expand
Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X

Senior editors

Editor(s)-in-Chief: Elekes, Károly

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

Acta Biologica Hungarica
P.O. Box 35
H-8237 Tihany, Hungary
Phone: (36 87) 448 244 ext. 103
Fax: (36 87) 448 006
E-mail: elekes@tres.blki.hu

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture
  • Biology and Environmental Sciences
  • Elsevier BIOBASE
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Acta Biologica Hungarica
Language English
Size  
Year of
Foundation		 1950
Publication
Programme		 changed title
Volumes
per Year		  
Issues
per Year		  
Founder Magyar Tudományos Akadémia
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Dec 2024 30 0 0
Jan 2025 41 0 0
Feb 2025 69 0 0
Mar 2025 51 0 0
Apr 2025 20 1 0
May 2025 7 0 0
Jun 2025 0 0 0