The downside of plant tissue culture techniques is an unwanted microbial contamination. Elimination of contaminants is the first step of any successful investigation on plant tissue culture. Preliminary experiments on Araucaria excelsa R. Br. var. glauca (Norfolk-Island pine) (syn.: A. heterophylla) showed that most common decontaminants could not successfully eliminate the contamination. Therefore, nano silver (NS) colloids were evaluated for controlling contamination. Treatments were included soaking the explants in NS solution or adding NS to the culture medium. Explants were cultured on MS medium supplemented with appropriate growth regulators for their establishment. Results showed that surface sterilization followed by treatment with 200 mg l−1 of NS with soaking time of 180 min reduced the bacterial contamination from 61.5% to 11.3% and adding 400 mg l−1 NS to the medium reduced the bacterial contamination from 81.25% to 18.75%. Nano silver could be applied without adverse effects on plant growth and development. This is the first report on in vitro establishment of A. excelsa R. Br. using NS to reduce bacterial infections.
Abdi, G. H., Salehi, H., Khosh-Khui, M. (2008) Nano silver: a novel nanomaterial for removal of bacterial contaminants in valerian (Valeriana officinalis L.) tissue culture. Acta Physiol. Plant. 30, 709–714.
Khosh-Khui M. , 'Nano silver: a novel nanomaterial for removal of bacterial contaminants in valerian (Valeriana officinalis L.) tissue culture ' (2008 ) 30 Acta Physiol. Plant. : 709 -714 .
Baker, C., Pradhan, A., Pakstis, L., Pochan, D. J., Shah, S. I. (2005) Synthesis and antibacterial properties of silver nanoparticles. J. Nanosci. Technol. 5, 244–249.
Shah S. I. , 'Synthesis and antibacterial properties of silver nanoparticles ' (2005 ) 5 J. Nanosci. Technol. : 244 -249 .
Batarseh, K. I. (2004) Anomaly and correlation of killing in the therapeutic properties of silver(I) chelating with glutamic and tartaric acids. J. Antimicrob. Chemoth. 54, 546–548.
Batarseh K. I. , 'Anomaly and correlation of killing in the therapeutic properties of silver(I) chelating with glutamic and tartaric acids ' (2004 ) 54 J. Antimicrob. Chemoth. : 546 -548 .
Bragg, P. D., Rannie, D. J. (1974) The effect of silver ions on the respiratory chain of E. coli. Can. J. Microbiol. 20, 883–889.
Rannie D. J. , 'The effect of silver ions on the respiratory chain of E. coli ' (1974 ) 20 Can. J. Microbiol. : 883 -889 .
Braydich-Stolle, L., Hussain, S., Schlager, J. J., Hofmann, M. C. (2005) In vitro cytotoxicity of nanoparticles in mammalian germline stem cells. Toxicol. Sci. 88, 412–419.
Hofmann M. C. , 'In vitro cytotoxicity of nanoparticles in mammalian germline stem cells ' (2005 ) 88 Toxicol. Sci. : 412 -419 .
Constantine, D. R. (1986) Micropropagation in the commercial environment. In: Withers, L., Alderson, P. G. (eds) Plant tissue culture and its agricultural applications. Butterworth, London, pp, 175–186.
Constantine D. R. , '', in Plant tissue culture and its agricultural applications , (1986 ) -.
Dodds, J. H., Roberts, W. L. (1981) Some inhibitory effectors on gentamicin on plant tissue culture. In Vitro 17, 467–470.
Roberts W. L. , 'Some inhibitory effectors on gentamicin on plant tissue culture ' (1981 ) 17 In Vitro : 467 -470 .
Eapen, S., George, L. (1997) Plant regeneration from peduncle segments of oil seed brassica species: influence of silver nitrate and silver thiosulfate. Plant Cell Tiss. Org. Cult. 51, 229–232.
George L. , 'Plant regeneration from peduncle segments of oil seed brassica species: influence of silver nitrate and silver thiosulfate ' (1997 ) 51 Plant Cell Tiss. Org. Cult. : 229 -232 .
Falkiner, F. R. (1990) The criteria for choosing an antibiotic for control of bacteria in plant tissue culture in TCL. Assoc. Plant Tiss. Cult. Newsl. 60, 13–23.
Falkiner F. R. , 'The criteria for choosing an antibiotic for control of bacteria in plant tissue culture in TCL ' (1990 ) 60 Assoc. Plant Tiss. Cult. Newsl. : 13 -23 .
Geong, Y., Hwang, H., Hi, S. C. (2005) Antibacterial properties of padded PP/PE nonwovens incorporating nano-sized silver. Colloids J. Mater. Sci. 40, 5413–5418.
Hi S. C. , 'Antibacterial properties of padded PP/PE nonwovens incorporating nano-sized silver ' (2005 ) 40 Colloids J. Mater. Sci. : 5413 -5418 .
Giri, C. C., Shyamkmar, B., Anjaneylnu, C. (2004) Progresses in tissue culture, genetic transformation and application of biotechnology to trees: an overview. Trees 18, 115–135.
Anjaneylnu C. , 'Progresses in tissue culture, genetic transformation and application of biotechnology to trees: an overview ' (2004 ) 18 Trees : 115 -135 .
Gyulai, G., R., Láposi, C., Herschbach, A., Veres, Gy., Fábián, L., Waters, Jr., Rennenberg, H. (2011) Conservation genetics (1710–2010) — Cloning of living fossils: Micropropagation of the oldest Hungarian black locust tree (Robinia pseudoacacia) planted in 1710 (Bábolna, Hungary). In: G. Gyulai, G. (ed.). Plant Archaeogenetics. Chapter 10. Nova Sci Publisher Inc., New York, USA.
Rennenberg H. , '', in Plant Archaeogenetics , (2011 ) -.
Harry, I. S., Thorpe, T. A. (1990) Special problems and prospects in the propagation of woody species, in plant aging basic and applied approaches. Plenum. New York, pp. 67–74.
Thorpe T. A. , '', in Special problems and prospects in the propagation of woody species, in plant aging basic and applied approaches , (1990 ) -.
Murashige, T., Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–497.
Skoog F. , 'A revised medium for rapid growth and bioassays with tobacco tissue cultures ' (1962 ) 15 Physiol. Plant. : 473 -497 .
Nomiya, K., Yoshizawa, A., Tsukagoshi, K., Kasuga, N. C., Hirakava, S., Watanabe, J. (2004) Synthesis and structural characterization of silver(I), aluminium(III) and cobalt(II) complexes with 4-isopropyltropolone (hinokitiol) showing noteworthy biological activities. Action of silver(I)-oxygen bonding complexes on the antimicrobial activities. J. Inorg. Biochem. 98, 46–60.
Watanabe J. , 'Synthesis and structural characterization of silver(I), aluminium(III) and cobalt(II) complexes with 4-isopropyltropolone (hinokitiol) showing noteworthy biological activities. Action of silver(I)-oxygen bonding complexes on the antimicrobial activities ' (2004 ) 98 J. Inorg. Biochem. : 46 -60 .
Park, H. J., Kim, S. H., Kim, H. J., Choi, S. (2006) A new composition of nanosized silica-silver for control of various plant diseases. Plant Pathol. J. 22, 295–302.
Choi S. , 'A new composition of nanosized silica-silver for control of various plant diseases ' (2006 ) 22 Plant Pathol. J. : 295 -302 .
Purnhauser, L., Medgyesy, P., Czakó, M., Dix, P. J., Márton, L. (1987) Stimulation of shoot regeneration in Triticum aestivum and Nicotiana plumbaginifolia Viv. tissue cultures using the ethylene inhibitor AgNO3. Plant Cell Report 6, 1–4.
Márton L. , 'Stimulation of shoot regeneration in Triticum aestivum and Nicotiana plumbaginifolia Viv. tissue cultures using the ethylene inhibitor AgNO3 ' (1987 ) 6 Plant Cell Report : 1 -4 .
Russell, A. D., Hugo, W. B. (1994) Antimicrobial activity and action of silver. Prog. Med. Chem. 31, 351–371.
Hugo W. B. , 'Antimicrobial activity and action of silver ' (1994 ) 31 Prog. Med. Chem. : 351 -371 .
Sarmast, M. K., Salehi, H., Khosh-Khui, M. (2009) Using plagiotropic shoot explant in tissue culture of Araucaria excelsa R. Br. var. glauca. Adv. Environ. Biol. 3, 194–194.
Khosh-Khui M. , 'Using plagiotropic shoot explant in tissue culture of Araucaria excelsa R. Br. var. glauca ' (2009 ) 3 Adv. Environ. Biol. : 194 -194 .
Sarmast, M. K., Salehi, H., Ramezani, A., Abolimoghadam, A. A., Niazi, A., Khosh-Khui, M. (2011) RAPD fingerprint to appraise the genetic fidelity of in vitro propagated Araucaria excelsa R. Br. var. glauca plantlets. Mol. Biotechnol. DOI 10.1007/s12033-011-9421-7.
Sehgal, L., Sehgal, O. P., Khosla, P. K. (1989) Micropropagation of Araucaria columnaris Hook. Ann. Sci. Forest 46, 158–160.
Khosla P. K. , 'Micropropagation of Araucaria columnaris Hook ' (1989 ) 46 Ann. Sci. Forest : 158 -160 .
Shrivastava, S., Bera, T., Roy, A., Singh, G., Ramachandrarao, P., Debabrata, D. (2007) Characterization of enhanced antibacterial effects of novel silver nanoparticles. Nanotechnology 18, 225103. (9 pp).
Debabrata D. , 'Characterization of enhanced antibacterial effects of novel silver nanoparticles ' (2007 ) 18 Nanotechnology : 225103 -.
Sondi, I., Salopek-Sondi, B. (2004) Silver nano particles as antimicrobial agent: A case study on E. coli as a model for Gram-negative bacteria. J. Colloid Interface Sci. 275, 177–182.
Salopek-Sondi B. , 'Silver nano particles as antimicrobial agent: A case study on E. coli as a model for Gram-negative bacteria ' (2004 ) 275 J. Colloid Interface Sci. : 177 -182 .
Taiz, L., Zeiger, E. (2006) Plant Physiology. Sinauer Assoc. Inc., 4 ed. 700 p.
Teixeira da Silva, G. A., Duong, T., Michi, T., Seiichi, F. (2003) The effect of antibiotics on the in vitro growth response of chrysanthemum and tobacco stem transverse thin cell layers (tTCLs). Sci. Hortic. 97, 397–410.
Seiichi F. , 'The effect of antibiotics on the in vitro growth response of chrysanthemum and tobacco stem transverse thin cell layers (tTCLs) ' (2003 ) 97 Sci. Hortic. : 397 -410 .
Wainwright, M., Grayston, S. J., de Jong, P. (1986) Adsorption of insoluble compounds by mycelium of the fungus Mucor flavus. Enzyme Micro. Technol. 8, 597–600.
Jong P. , 'Adsorption of insoluble compounds by mycelium of the fungus Mucor flavus ' (1986 ) 8 Enzyme Micro. Technol. : 597 -600 .
Zhang, P., Phansiri, S., Kaerlas, J. P. (2001) Improvement of cassava shoot organogenesis by the use of silver nitrate in vitro. Plant Cell Tiss. Org. Cult. 67, 47–54.
Kaerlas J. P. , 'Improvement of cassava shoot organogenesis by the use of silver nitrate in vitro ' (2001 ) 67 Plant Cell Tiss. Org. Cult. : 47 -54 .
Zhang, W., Qiao, X., Chen, J. (2007) Synthesis of nanosilver. Colloids and Surfaces A: Phys. Eng. Asp. 299, 22–28.
Chen J. , 'Synthesis of nanosilver ' (2007 ) 299 Colloids and Surfaces A: Phys. Eng. Asp. : 22 -28 .