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  • 1 Siedlce University of Natural Sciences and Humanities Department of Biochemistry and Molecular Biology ul. Prusa 12 08-110 Siedlce Poland
  • 2 Siedlce University of Natural Sciences and Humanities Department of Zoology ul. Prusa 12 08-110 Siedlce Poland
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The insecticidal activity of plant lectins against a wide range of insect species have been intensively studied. Understanding the mechanism of the toxicity of lectins is one of the studied aspects. In the present research, the first step was determine the effect of phytohemagglutinin (PHA) on the development, fecundity and mortality of grain aphid. Next, the effect of PHA lectin on the activity of such enzymes as: α- and β-glucosidases, alkaline (AkP) and acid (AcP) phosphatases, aminopeptidase N and cathepsin L involved in the metabolism of sugar, phosphorus and proteins of an adult apterae aphids was investigated. The PHA lectin added into the liquid diet increased the pre-reproductive period, mortality of Sitobion avenae, the time of generation development and decreased its fecundity and the intrinsic rate of natural increase. In addition, activity of α-glucosidase, alkaline phosphatase and aminopeptidase N of adult apterae exposed to PHA were reduced. The results indicate that the insecticidal activity of PHA on S. avenae may involve changes in activity of the enzymes in the midgut and it may be part of its toxicity.

  • Baker, J. E., Woo, S. M. (1981) Properties and specificities of a digestive aminopeptidase from larvae of Attagenus megatoma (Coleoptera: dermestidae). Comp. Biochem. Physiol. 69,2, 189–193.

    Woo S. M. , 'Properties and specificities of a digestive aminopeptidase from larvae of Attagenus megatoma (Coleoptera: dermestidae) ' (1981 ) 69 Comp. Biochem. Physiol. : 189 -193.

    • Search Google Scholar
  • Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248–254.

    Bradford M. M. , 'A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding ' (1976 ) 72 Anal. Biochem. : 248 -254.

    • Search Google Scholar
  • Carlini, C. R., Grossi-de-Sa’, M. F. (2002) Plant toxic proteins with insecticidal properties. A review on their potentialities as bioinsecticides. Toxicon 40, 1515–1539.

    Grossi-de-Sa’ M. F. , 'Plant toxic proteins with insecticidal properties. A review on their potentialities as bioinsecticides ' (2002 ) 40 Toxicon : 1515 -1539.

    • Search Google Scholar
  • Chararas, C., Chipoulet, J.-M. (1982) Purification by chromatography and properties of β-glucosidase from the larvae of Phoracantha semipunctata. Comp. Biochem. Physiol. Part B 72, 559–564.

    Chipoulet J.-M. , 'Purification by chromatography and properties of β-glucosidase from the larvae of Phoracantha semipunctata ' (1982 ) 72 Comp. Biochem. Physiol. Part B : 559 -564.

    • Search Google Scholar
  • Chen, M., Han, H., Qiao, X., Qu, M. (2007) Resistance mechanisms and associated mutations in acetylcholinesterase genes in Sitobion avenae (Fabiricius). Pestic. Biochem. Physiol. 87, 189–195.

    Qu M. , 'Resistance mechanisms and associated mutations in acetylcholinesterase genes in Sitobion avenae (Fabiricius) ' (2007 ) 87 Pestic. Biochem. Physiol. : 189 -195.

    • Search Google Scholar
  • Correia, M. T. S., Coelho, L. C. B. B., Paiva, P. M. G. (2008) Lectins, carbohydrate recognition molecules: are they toxic?. In: Siddique, Y. H. (ed.) Recent Trends in Toxicology. Transworld Research Network, Kerala. vol. 37, pp. 47–59.

  • Cristofoletti, P. T., Mendonca de Sousa, F. A., Rahbe, Y., Terra, W. R. (2006) Characterization of membrane-bound aminopeptidase purified from Acyrthosiphon pisum midgut cells. FEBS Journal, 273, 5574–5588.

    Terra W. R. , 'Characterization of membrane-bound aminopeptidase purified from Acyrthosiphon pisum midgut cells ' (2006 ) 273 FEBS Journal : 5574 -5588.

    • Search Google Scholar
  • Deloach, J. R., Mayer, R. T. (1979) The pupal instar of Stomoxys calcitrans: Developmental changes in acid phosphatase, cytochrome oxidase and lysosomal glycosidases. Insect Biochem. 9, 653–659.

    Mayer R. T. , 'The pupal instar of Stomoxys calcitrans: Developmental changes in acid phosphatase, cytochrome oxidase and lysosomal glycosidases ' (1979 ) 9 Insect Biochem. : 653 -659.

    • Search Google Scholar
  • Erlanger, B. F., Kokowsky, N., Cohen, W. (1961) The preparation and properties of two new chromogenic substances of trypsin. Arch. Biochem. Biophys. 95, 271–278.

    Cohen W. , 'The preparation and properties of two new chromogenic substances of trypsin ' (1961 ) 95 Arch. Biochem. Biophys. : 271 -278.

    • Search Google Scholar
  • Fitches, E., Gatehouse, J. A. (1998) A comparison of the short and long term effects of insecticidal lectins on activities of soluble and brush border enzymes of tomato moth larvae (Lacanobia oleracea). J. Insect. Physiol. 44, 1213–1224.

    Gatehouse J. A. , 'A comparison of the short and long term effects of insecticidal lectins on activities of soluble and brush border enzymes of tomato moth larvae (Lacanobia oleracea) ' (1998 ) 44 J. Insect. Physiol. : 1213 -1224.

    • Search Google Scholar
  • Fitches, E., Ilett, C., Gatehouse, A. M. R., Gatehouse, L. N., Greene, R., Edwards, J. P., Gatehouse, J. A. (2001) The effects of Phaseolus vulgaris erythro- and leucoagglutinating isolectins (PHA-E and PHA-L) delivered via artificial diet and transgenic plants on the growth and development of tomato moth (Lacanobia oleracea) larvae; lectin binding to gut glycoproteins in vitro and in vivo. J. Insect Physiol. 47, 1389–1398.

    Gatehouse J. A. , 'The effects of Phaseolus vulgaris erythro- and leucoagglutinating isolectins (PHA-E and PHA-L) delivered via artificial diet and transgenic plants on the growth and development of tomato moth (Lacanobia oleracea) larvae; lectin binding to gut glycoproteins in vitro and in vivo ' (2001 ) 47 J. Insect Physiol. : 1389 -1398.

    • Search Google Scholar
  • Gatehouse, A. M. R., Shackley, S. J., Fenton, K. A., Bryden, J. (1989) Mechanism of seed lectin tolerance by a major insect storage pest of Phaseolus vulgaris, Acanthoscelides obtectus. J. Sci. Food Agric. 47, 269–280.

    Bryden J. , 'Mechanism of seed lectin tolerance by a major insect storage pest of Phaseolus vulgaris, Acanthoscelides obtectus ' (1989 ) 47 J. Sci. Food Agric. : 269 -280.

    • Search Google Scholar
  • Goławska, S., Łukasik, I. (2007) Acceptance of low-saponin lines of alfalfa with varied phenolic concentrations by pea aphid (Homoptera: Aphididae). Biologia 64, 377–382.

    Łukasik I. , 'Acceptance of low-saponin lines of alfalfa with varied phenolic concentrations by pea aphid (Homoptera: Aphididae) ' (2007 ) 64 Biologia : 377 -382.

    • Search Google Scholar
  • Habibi, J., Backus, E. A., Czapla, T. H. (1993) Plant lectins effect survival of the potato leafhopper (Homoptera, Cicadellidae). J. Econ. Entomol. 86, 945–957.

    Czapla T. H. , 'Plant lectins effect survival of the potato leafhopper (Homoptera, Cicadellidae) ' (1993 ) 86 J. Econ. Entomol. : 945 -957.

    • Search Google Scholar
  • Katagiri, C. (1979) α-D-glucosidase in the serum of American cockroach, Periplaneta Americana. Insect Biochem. 9, 199–204.

    Katagiri C. , 'α-D-glucosidase in the serum of American cockroach, Periplaneta Americana ' (1979 ) 9 Insect Biochem. : 199 -204.

    • Search Google Scholar
  • Kieckhefer, R. W., Derr, R. F. (1976) Rearing three species of cereal aphids on artifical diets. J. Econ. Entomol. 60, 663–665.

    Derr R. F. , 'Rearing three species of cereal aphids on artifical diets ' (1976 ) 60 J. Econ. Entomol. : 663 -665.

    • Search Google Scholar
  • Kaur, M., Singh, K., Rup, P. J., Kamboj, S. S., Saxena, A. K., Sharma, M., Bhagat, M., Sood, S. K., Singh, J. (2006) A tuber lectin from Arisaema helleborifolium Schott with anti-insect and anti-proliferative properties. J. Biochem. Mol. Biol. 4, 432–440.

    Singh J. , 'A tuber lectin from Arisaema helleborifolium Schott with anti-insect and anti-proliferative properties ' (2006 ) 4 J. Biochem. Mol. Biol. : 432 -440.

    • Search Google Scholar
  • Macedo, M. L. R., Freire, M. G. M., Silva, M. B. R., Coelho, L. C. B. B. (2007) Insecticidal action of Bauhinia monandra leaf lectin (BmoLL) against Anagasta kuehniella (Lepidoptera: Pyralidae), Zabrotes subfasciatus and Callosobruchus maculates (Coleoptera: Bruchidae). Comp. Biochem. Physiol. A, 146, 486–498.

    Coelho L. C. B. B. , 'Insecticidal action of Bauhinia monandra leaf lectin (BmoLL) against Anagasta kuehniella (Lepidoptera: Pyralidae), Zabrotes subfasciatus and Callosobruchus maculates (Coleoptera: Bruchidae) ' (2007 ) 146 Comp. Biochem. Physiol. A : 486 -498.

    • Search Google Scholar
  • Machuka, J., Van Damme, E. J. M., Peumans, W. J., Jackai, L. E. N. (1999) Effect on plant lectins on larval development of the legume pod border Maruca vitrata. Entomol. Exp. Appl. 93, 179–187.

    Jackai L. E. N. , 'Effect on plant lectins on larval development of the legume pod border Maruca vitrata ' (1999 ) 93 Entomol. Exp. Appl. : 179 -187.

    • Search Google Scholar
  • Matsushita, H., Takenaka, M., Ogawa. H. (2002) Porcine pancreatic α-amylase shows binding activity toward N-linked oligosaccharides of glicoproteins. J. Biol. Chem. 227, 4680–4686.

    Ogawa H. , 'Porcine pancreatic α-amylase shows binding activity toward N-linked oligosaccharides of glicoproteins ' (2002 ) 227 J. Biol. Chem. : 4680 -4686.

    • Search Google Scholar
  • Němec, V., Ženka, J. (1996) Activity of phosphatases and esterases in the aphid, Acyrthosiphon pisum (Hemiptera: Sternorrhyncha: Aphididae), and in the gut wall of Galleria mellonella (Lepidoptera: Pyralidae) larvae and pupae. Eur. J. Entomol. 93, 37–44.

    Ženka J. , 'Activity of phosphatases and esterases in the aphid, Acyrthosiphon pisum (Hemiptera: Sternorrhyncha: Aphididae), and in the gut wall of Galleria mellonella (Lepidoptera: Pyralidae) larvae and pupae ' (1996 ) 93 Eur. J. Entomol. : 37 -44.

    • Search Google Scholar
  • Neumann, H., van Vreedendaal, M. (1967) An improved alkaline phosphatase determination with p-nitrophenyl phosphate. Clin. Chim. Acta 17, 183–187.

    Vreedendaal M. , 'An improved alkaline phosphatase determination with p-nitrophenyl phosphate ' (1967 ) 17 Clin. Chim. Acta : 183 -187.

    • Search Google Scholar
  • Price, D. R. G., Karley, A. J., Ashford, D. A., Isaacs, H. V., Pownall, M. E., Wilkinson, H. S., Gatehouse, J. A., Douglas, A. E. (2007) Molecular characterisation of candidate gut sucrase in the pea aphid Acyrthosiphon pisum. Insect Biochem. Mol. Biol. 37, 307–317.

    Douglas A. E. , 'Molecular characterisation of candidate gut sucrase in the pea aphid Acyrthosiphon pisum ' (2007 ) 37 Insect Biochem. Mol. Biol. : 307 -317.

    • Search Google Scholar
  • Pyati, P., Bandani, A. R., Fitches, E., Gatehouse, J. A. (2011) Protein digestion in cereal aphids (Sitobion avenae) as a target for plant defence by endogenous proteinase inhibitors. J. Insect Physiol. 57, 881–891.

    Gatehouse J. A. , 'Protein digestion in cereal aphids (Sitobion avenae) as a target for plant defence by endogenous proteinase inhibitors ' (2011 ) 57 J. Insect Physiol. : 881 -891.

    • Search Google Scholar
  • Rabossi, A., Ación, L., Quesada-Allué, L. A. (2000) Metamorphosis-associated proteolysis in Ceratitis capitata. Entomol. Exp. Appl. 94, 57–65.

    Quesada-Allué L. A. , 'Metamorphosis-associated proteolysis in Ceratitis capitata ' (2000 ) 94 Entomol. Exp. Appl. : 57 -65.

    • Search Google Scholar
  • Rahbe, Y., Sauvion, N., Febvay, G., Peumans, W. J., Gatehouse, A. M. R. (1995) Toxocity of lectins and processing of ingested proteins in the pea aphid Acyrthosiphon pisum. Entomol. Exp. Appl. 76, 143–155.

    Gatehouse A. M. R. , 'Toxocity of lectins and processing of ingested proteins in the pea aphid Acyrthosiphon pisum ' (1995 ) 76 Entomol. Exp. Appl. : 143 -155.

    • Search Google Scholar
  • Rees, H. H. (1995) Ecdysteroid biosynthesis and inactivation in relation to function. Eur. J. Entomol. 92, 9–39.

    Rees H. H. , 'Ecdysteroid biosynthesis and inactivation in relation to function ' (1995 ) 92 Eur. J. Entomol. : 9 -39.

    • Search Google Scholar
  • Sadeghi, A., Van Damme, E. J. M., Peumans, W. J., Smagghe, G. (2006) Deterrent activity of plant lectins on cowpea weevil Callosobruchus maculatus (F.) oviposition. Phytochemistry 67, 2078–2084.

    Smagghe G. , 'Deterrent activity of plant lectins on cowpea weevil Callosobruchus maculatus (F.) oviposition ' (2006 ) 67 Phytochemistry : 2078 -2084.

    • Search Google Scholar
  • Sauvion, N., Charles, H., Febvay, G., Rahbe, Y. (2003) Effects of jackbean lectin (ConA) on the feeding behaviour and kinetics of intoxication of the pea aphid, Acyrthosiphon pisum. Entomol. Exp. Appl. 110, 31–44.

    Rahbe Y. , 'Effects of jackbean lectin (ConA) on the feeding behaviour and kinetics of intoxication of the pea aphid, Acyrthosiphon pisum ' (2003 ) 110 Entomol. Exp. Appl. : 31 -44.

    • Search Google Scholar
  • Singh, K., Kaur, M., Rup, M. K., Singh, J. (2006) Exporation for anti-insect properties of lectin from seeds of soyabean, Glycine max L. using Bactrocera cucurbitae ( Coquillett) as model. Phytoparasitica 34, 463–473.

    Singh J. , 'Exporation for anti-insect properties of lectin from seeds of soyabean, Glycine max L. using Bactrocera cucurbitae ( Coquillett) as model ' (2006 ) 34 Phytoparasitica : 463 -473.

    • Search Google Scholar
  • Singh, K., Kaur, M., Rup, M., Singh, J. (2008) Effects of plant lectin from cobra lily, Arisaema Curvatum Kunth on development of melon fruitly, Bactrocera cucurbitae (Coq.). J. Environ. Biol. 29, 911–916.

    Singh J. , 'Effects of plant lectin from cobra lily, Arisaema Curvatum Kunth on development of melon fruitly, Bactrocera cucurbitae (Coq.) ' (2008 ) 29 J. Environ. Biol. : 911 -916.

    • Search Google Scholar
  • Sprawka, I., Leszczyński, B. (2007) Impact of lectin ( phytohemagglutinin) isolated from beans on grain aphid Sitobion avenae (F.) bionomy. Aphids and Other Hemipterous Insects 13, 157–165.

    Leszczyński B. , 'Impact of lectin ( phytohemagglutinin) isolated from beans on grain aphid Sitobion avenae (F.) bionomy ' (2007 ) 13 Aphids and Other Hemipterous Insects : 157 -165.

    • Search Google Scholar
  • Sprawka, I., Goławska, S. ( 2010) Effect of the lectin PHA on the feeding behavior of the grain aphid. J. Pest Sci. 83, 149–155.

    Goławska S. , 'Effect of the lectin PHA on the feeding behavior of the grain aphid ' (2010 ) 83 J. Pest Sci. : 149 -155.

    • Search Google Scholar
  • StatSoft Inc. (2003) Statistica (Data Analysis Software System), version 07. www.statsoft.com

  • Terra, W. R., Ferreira, C., Jordao, B. P., Dillon, R. J. (1996) Digestive enzymes. In: Lehane, M. J., Billingsley, P. F. (eds) Biology of the insect midgut. Chapman & Hall, London. pp. 153–193.

    Dillon R. J. , '', in Biology of the insect midgut , (1996 ) -.

  • Vasconcelos, I. M., Oliveira, J. T. A. (2004) Antinutritional properties of plant lectins. Toxicon 44, 1737–1747.

    Oliveira J. T. A. , 'Antinutritional properties of plant lectins ' (2004 ) 44 Toxicon : 1737 -1747.

    • Search Google Scholar
  • Wagner, W., Mohrlen, F., Schnetter, W. (2002) Characterization of the proteolytic enzymes in the midgut of the European Cockchafer, Melontha melontha (Coleoptera: Scarabaeidae). Insect Biochem. Mol. Biol. 32, 803–814.

    Schnetter W. , 'Characterization of the proteolytic enzymes in the midgut of the European Cockchafer, Melontha melontha (Coleoptera: Scarabaeidae) ' (2002 ) 32 Insect Biochem. Mol. Biol. : 803 -814.

    • Search Google Scholar
  • Wyatt, I., White, P. F. (1977) Simple estimation of intrinsic increase rates for aphids and tetranychid mites. J. Appl. Ecol. 14, 757–776.

    White P. F. , 'Simple estimation of intrinsic increase rates for aphids and tetranychid mites ' (1977 ) 14 J. Appl. Ecol. : 757 -776.

    • Search Google Scholar
  • Zhao, L. Y., Chen, J. L., Cheng, D. F., Sun, J. R., Liu, Y., Tian, Z. (2009) Biochemical and molecular characterization of Sitobion avenae — induced wheat defense responses. Crop Prot. 28, 435–444.

    Tian Z. , 'Biochemical and molecular characterization of Sitobion avenae — induced wheat defense responses ' (2009 ) 28 Crop Prot. : 435 -444.

    • Search Google Scholar