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  • 1 Chongqing University Key Laboratory of Biorheological Science and Technology, Ministry of Education, College of Bioengineering Chongqing China
  • 2 Chongqing Three Gorges University School of Life Science and Engineering Chongqing China
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Using uniform random design optimization and the mathematical model equation we optimized the regeneration tissue culture system of the chilli pepper. An efficient and detailed plant reproducible protocol in vitro has been established using different explants and induction media for three chilli pepper cultivars. The result displayed that the seedlings at the curved hypocotyl stage were the best choice to prepare for explants, the genotype of explants affected shoot buds induction frequency and number of shoot buds per explant, and the cotyledon explant was more responsive than hypocotyl explant. The optimal media for maximum shoot initiation and regeneration and the optimal elongation medium were obtained. For Capsicum annuum var. annuum (cv. Xinsu), Capsicum annuum var. annuum (cv. Neimengchifeng) and Capsicum frutescens (cv. Xingfu), the induction rates were 99.17%, 97.50 and 96.11%, respectively; the elongation rates of shoot buds were 86.67%, 85.19% and 82.96%, respectively. The MS medium with 0.57 μM IAA and 0.69 μM NAA is the best choice for root induction. The frequency of their root emergence was 95.00–98.33%. Regenerated chilli peppers were successfully acclimatized and cultivated with 100% survival. This work will help to improve multiplication process and the genotype of chilli pepper, and may have commercial impact.

  • Agrawal, S., Chandra, N., Kothari, S. L. (1989) Plant regeneration in tissue cultures of pepper (Capsicum annuum L. cv. Mathania). Plant Cell Tiss. Org. Cult. 16, 47–55.

    Kothari S. L. , 'Plant regeneration in tissue cultures of pepper (Capsicum annuum L. cv. Mathania) ' (1989 ) 16 Plant Cell Tiss. Org. Cult. : 47 -55.

    • Search Google Scholar
  • Chi, G. L., Barfield, D. G., Sim, G. E., Pua, E. C. (1990) Effect of AgNO3 and aminoethoxyglycine on in vitro shoot and root organogenesis from seedling explants of recalcitrant Brassica genotypes. Plant Cell Rep. 9, 195–198.

    Pua E. C. , 'Effect of AgNO3 and aminoethoxyglycine on in vitro shoot and root organogenesis from seedling explants of recalcitrant Brassica genotypes ' (1990 ) 9 Plant Cell Rep. : 195 -198.

    • Search Google Scholar
  • Christopher, T., Rajam, M. V. (1996) Effect of genotype, explant and medium on in vitro regeneration of red pepper. Plant Cell Tiss. Org. Cult. 46, 245–250.

    Rajam M. V. , 'Effect of genotype, explant and medium on in vitro regeneration of red pepper ' (1996 ) 46 Plant Cell Tiss. Org. Cult. : 245 -250.

    • Search Google Scholar
  • DeWitt, D., Bosland, P. W. (1993) The Pepper Garden. Ten Speed Press, Berkeley, CA, pp. 240.

    Bosland P. W. , '', in The Pepper Garden , (1993 ) -.

  • Feyissa, T., Welander, M., Negash, L. (2005) In vitro regeneration of Hagenia abyssinica (Bruce) J. F. Gmel. (Rosaceae) from leaf explants. Plant Cell Rep. 24, 392–400.

    Negash L. , 'In vitro regeneration of Hagenia abyssinica (Bruce) J. F. Gmel. (Rosaceae) from leaf explants ' (2005 ) 24 Plant Cell Rep. : 392 -400.

    • Search Google Scholar
  • Gautam, V. K., Mittal, A., Nanda, K., Gupta, S. C. (1983) In vitro regeneration of plantlets from somatic explants of matthiola-incana. Plant Sci. Lett. 29, 25–32.

    Gupta S. C. , 'In vitro regeneration of plantlets from somatic explants of matthiola-incana ' (1983 ) 29 Plant Sci. Lett. : 25 -32.

    • Search Google Scholar
  • Goldfarb, B., Howe, G. T., Bailey, L. M., Strauss, S. H., Zaerr, J. B. (1991) A liquid cytokinin pulse induces adventitious shoot formation from Douglas-fir cotyledons. Plant Cell Rep. 10, 156–160.

    Zaerr J. B. , 'A liquid cytokinin pulse induces adventitious shoot formation from Douglas-fir cotyledons ' (1991 ) 10 Plant Cell Rep. : 156 -160.

    • Search Google Scholar
  • Greenleaf, W. H. (1986) Pepper breeding. In: Basset, M. J. (ed.) Breeding Vegetable Crops. Avi Publishing Company Inc., Westport, Connecticut, pp. 67–134.

    Greenleaf W. H. , '', in Breeding Vegetable Crops , (1986 ) -.

  • Hyde, C., Phillips, G. C. (1996) Silver nitrate promotes shoot development and plant regeneration on chile pepper (Capsicum annuum L.) via organogenesis. In Vitro Cell. Dev. Biol. Plant 32, 72–80.

    Phillips G. C. , 'Silver nitrate promotes shoot development and plant regeneration on chile pepper (Capsicum annuum L.) via organogenesis ' (1996 ) 32 In Vitro Cell. Dev. Biol. Plant : 72 -80.

    • Search Google Scholar
  • Kothari, S. L., Joshi, A., Kachhwaha, S., Ochoa-Alejo, N. (2010) Chilli peppers — a review on tissue culture and transgenesis. Biotechnol. Adv. 28, 35–48.

    Ochoa-Alejo N. , 'Chilli peppers — a review on tissue culture and transgenesis ' (2010 ) 28 Biotechnol. Adv. : 35 -48.

    • Search Google Scholar
  • Lane, H. C., Hesketh, J. D. (1977) Cotyledon photosynthesis during seedling growth of cotton, Gossypium mirsutum L. Am. J. Bot. 64, 786–790.

    Hesketh J. D. , 'Cotyledon photosynthesis during seedling growth of cotton, Gossypium mirsutum L ' (1977 ) 64 Am. J. Bot. : 786 -790.

    • Search Google Scholar
  • Lee, Y. H., Kim, H. S., Kim, J. Y., Jung, M., Park, Y. S., Lee, J. S., Choi, S. H., Her, N. H., Lee, J. H., Hyung, N. I., Lee, C. H., Yang, S. G., Harn, C. H. (2004) A new selection method for pepper transformation: callus-mediated shoot formation. Plant Cell Rep. 23, 50–58.

    Harn C. H. , 'A new selection method for pepper transformation: callus-mediated shoot formation ' (2004 ) 23 Plant Cell Rep. : 50 -58.

    • Search Google Scholar
  • Mezghani, N., Jemmali, A., Elloumi, N., Gargouri-Bouzid, R., Kintzios, S. (2007) Morphohistological study on shoot bud regeneration in cotyledon cultures of pepper (Capsicum annuum). Biol. Bratislava 62, 704–710.

    Kintzios S. , 'Morphohistological study on shoot bud regeneration in cotyledon cultures of pepper (Capsicum annuum) ' (2007 ) 62 Biol. Bratislava : 704 -710.

    • Search Google Scholar
  • Ochoa-Alejo, N., Ireta-Moreno, L. (1990) Cultivar differences in shoot-forming capacity of hypocotyl tissues of chilli pepper (Capsicum annuum L.) cultured in vitro. Sci. Hortic. 42, 21–28.

    Ireta-Moreno L. , 'Cultivar differences in shoot-forming capacity of hypocotyl tissues of chilli pepper (Capsicum annuum L.) cultured in vitro ' (1990 ) 42 Sci. Hortic. : 21 -28.

    • Search Google Scholar
  • Ochoa-Alejo, N., Ramírez-Malagón, R. (2001) In vitro chilli pepper biotechnology. In Vitro Cell. Dev. Biol. Plant 37, 701–729.

    Ramírez-Malagón R. , 'In vitro chilli pepper biotechnology ' (2001 ) 37 In Vitro Cell. Dev. Biol. Plant : 701 -729.

    • Search Google Scholar
  • Sa, G., Mi, M., He-chun, Y., Ben-ye, L., Guo-feng, L., Kang, C. (2001) Effects of ipt gene expression on the physiological and chemical characteristics of Artemisia annua L. Plant Sci. 160, 691–698.

    Kang C. , 'Effects of ipt gene expression on the physiological and chemical characteristics of Artemisia annua L ' (2001 ) 160 Plant Sci. : 691 -698.

    • Search Google Scholar
  • Schween, G., Schwenkel, H. G. (2003) Effect of genotypes on callus induction, shoot regeneration, and phenotypic stability of regenerated plants in the greenhouse of Primula ssp. Plant Cell Tiss. Org. Cult. 72, 53–61.

    Schwenkel H. G. , 'Effect of genotypes on callus induction, shoot regeneration, and phenotypic stability of regenerated plants in the greenhouse of Primula ssp ' (2003 ) 72 Plant Cell Tiss. Org. Cult. : 53 -61.

    • Search Google Scholar
  • Sripichitt, P., Nawata, E., Shigenaga, S. (1987) In vitro shoot-forming capacity of cotyledon explants in red pepper (Capsicum annuum L. cv. Yatsufusa). Jap. J. Breed. 37, 133–142.

    Shigenaga S. , 'In vitro shoot-forming capacity of cotyledon explants in red pepper (Capsicum annuum L. cv. Yatsufusa) ' (1987 ) 37 Jap. J. Breed. : 133 -142.

    • Search Google Scholar