Acta Biologica Hungarica
Volume/Issue:
Volume 64: Issue 2
Enzymatic and non-enzymatic antioxidant responses of alfalfa leaves and roots under different salinity levels
Authors:
G. Dehghan University of Tabriz Department of Plant Biology, Faculty of Natural Science Tabriz Iran

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Leyla Amjad University of Tabriz Department of Plant Biology, Faculty of Natural Science Tabriz Iran

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H. Nosrati University of Tabriz Department of Plant Biology, Faculty of Natural Science Tabriz Iran

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Pages:
207–217
Online Publication Date:
05 Jun 2013
Publication Date:
01 Jun 2013
Article Category:
Research Article
DOI:
https://doi.org/10.1556/abiol.64.2013.2.7
Keywords:
Medicago sativa L.; Gara-Yonjeh ; antioxidant system; salt stress; isoenzyme pattern
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The effect of increasing NaCl concentrations on biomass, hydrogen peroxide (H2O2), ascorbic acid (ASC), proline and total thiol, and the activity of some antioxidant enzymes in alfalfa (Medicago sativa L. cv. Gara-Yonjeh) were investigated. The dry weights of roots and shoots with increasing NaCl concentrations decreased progressively, and the strongest toxicity was detected at NaCl treatment of 200 mM. Superoxide dismutase (SOD) activity in the leaves increased gradually up to NaCl concentrations of 100, while the higher concentration of NaCl reduced SOD activity in both leaves and roots. The maximum levels of ascorbate peroxidase (APX) activity were increased at 150 mM and 100 mM NaCl in leaves and roots of Gara-Yonjeh, respectively. Peroxidase (POD) activity in roots of Gara-Yonjeh increased (82% at 200 mM) by salinity, while it decreased (43% at 200 mM) in leaves. In contrast, catalase (CAT) activitiy increased (84% at 200 mM) in leaves, and decreased (57% at 200 mM) in the roots of Gara-Yonjeh. Electrophoresis analysis suggested that different patterns in SOD, CAT and POD isoenzymes depend on NaCl concentrations, and the staining intensities of these isoforms are supported the results obtained from the spectrophotometric determinations. In POD and CAT, activity of isoform III was detected at all concentrations, by a “low-high-low” pattern, with the maximum activity at 50 mM of NaCl. Results imply that the function of antioxidant systems in higher NaCl concentration is responsible for the salt tolerance observed in Gara-Yonjeh.

  • Badawi, G. H., Yamauchi, Y., Shimada, E., Sasaki, R., Kawano, N., Tanaka, K., Tanaka, K. (2004) Enhanced tolerance to salt stress and water deficit by overexpressing superoxide dismutase in tobacco (Nicotiana tabacum) chloroplasts. Plant Sci. 166, 919–928.

    Tanaka K. , 'Enhanced tolerance to salt stress and water deficit by overexpressing superoxide dismutase in tobacco (Nicotiana tabacum) chloroplasts ' (2004 ) 166 Plant Sci. : 919 -928 .

    • Search Google Scholar

  • Bartels, D., Sunkar, R. (2005) Drought and salt tolerance in plants. Crit. Rev. Plant Sci. 24, 23–58.

    Sunkar R. , 'Drought and salt tolerance in plants ' (2005 ) 24 Crit. Rev. Plant Sci. : 23 -58 .

    • Search Google Scholar

  • Bates, L. S., Waldren, R. P., Teare, I. D. (1973) Rapid determination of free proline for water stress studies. Plant Soil 39, 205–207.

    Teare I. D. , 'Rapid determination of free proline for water stress studies ' (1973 ) 39 Plant Soil : 205 -207 .

    • Search Google Scholar

  • Beauchamp, C., Fridovich, I. (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44, 276–287.

    Fridovich I. , 'Superoxide dismutase: improved assays and an assay applicable to acrylamide gels ' (1971 ) 44 Anal. Biochem. : 276 -287 .

    • Search Google Scholar

  • Boominathan, R., Doran, P. M. (2002) Ni-induced oxidative stress in roots of the Ni hyperaccumulator, Alyssum bertolonii. New Phytol. 156, 205–215.

    Doran P. M. , 'Ni-induced oxidative stress in roots of the Ni hyperaccumulator, Alyssum bertolonii ' (2002 ) 156 New Phytol. : 205 -215 .

    • Search Google Scholar

  • Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Annu. Rev. Biochem. 72, 248–254.

    Bradford M. M. , 'A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding ' (1976 ) 72 Annu. Rev. Biochem. : 248 -254 .

    • Search Google Scholar

  • Cobbett, C. S. (2000) Phytochelatins biosynthesis and function in heavy-metal detoxification. Curr. Opin. Plant Biol. 3, 211–216.

    Cobbett C. S. , 'Phytochelatins biosynthesis and function in heavy-metal detoxification ' (2000 ) 3 Curr. Opin. Plant Biol. : 211 -216 .

    • Search Google Scholar

  • Ellman, G. L. (1959) Tissue sulfhydryl groups. Arch. Biochem. Biophys. 82, 70–77.

    Ellman G. L. , 'Tissue sulfhydryl groups ' (1959 ) 82 Arch. Biochem. Biophys. : 70 -77 .

    • Search Google Scholar

  • Ghamsari, L., Keyhani, E., Golkhoo, S. (2007) Kinetics properties of guaiacol peroxidase activity in Crocus sativus L. corm during rooting. Iran Biomed. J. 11, 137–146.

    Golkhoo S. , 'Kinetics properties of guaiacol peroxidase activity in Crocus sativus L. corm during rooting ' (2007 ) 11 Iran Biomed. J. : 137 -146 .

    • Search Google Scholar

  • Hajiboland, R., Amjad, L. (2007) Does antioxidant capacity of leaves play a role in growth response to selenium at different sulfur nutritional status? Plant Soil Environ. 53, 207–215.

    Amjad L. , 'Does antioxidant capacity of leaves play a role in growth response to selenium at different sulfur nutritional status? ' (2007 ) 53 Plant Soil Environ. : 207 -215 .

    • Search Google Scholar

  • Howieson, J., Ballard, R. (2004) Optimising the legume symbiosis in stressful and competitive environments within southern Australia: some contemporary thoughts. Soil Biol. Biochem. 36, 1261–1273.

    Ballard R. , 'Optimising the legume symbiosis in stressful and competitive environments within southern Australia: some contemporary thoughts ' (2004 ) 36 Soil Biol. Biochem. : 1261 -1273 .

    • Search Google Scholar

  • Hu, M. L. (1994) Measurement of protein thiol groups and glutathione in plasma. Methods Enzymol. 233, 380–385.

    Hu M. L. , 'Measurement of protein thiol groups and glutathione in plasma ' (1994 ) 233 Methods Enzymol. : 380 -385 .

    • Search Google Scholar

  • Jebara, S., Jebara, M., Limam, F., Aouani, M. E. (2005) Changes in ascorbate peroxidase, catalase, guaiacol peroxidase and superoxide dismutase activities in common bean (Phaseolus vulgaris) nodules under salt stress. J. Plant. Physiol. 162, 929–936.

    Aouani M. E. , 'Changes in ascorbate peroxidase, catalase, guaiacol peroxidase and superoxide dismutase activities in common bean (Phaseolus vulgaris) nodules under salt stress ' (2005 ) 162 J. Plant. Physiol. : 929 -936 .

    • Search Google Scholar

  • Kim, S. Y., Lim, J. H., Park, M. R., Kim, Y. J., Park, T. I. I., Seo, Y. W. (2005) Enhanced antioxidant enzymes are associated with reduced hydrogen peroxide in barley roots under salt stress. J. Biochem. Mol. Biol. 38, 218–224.

    Seo Y. W. , 'Enhanced antioxidant enzymes are associated with reduced hydrogen peroxide in barley roots under salt stress ' (2005 ) 38 J. Biochem. Mol. Biol. : 218 -224 .

    • Search Google Scholar

  • Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.

    Laemmli U. K. , 'Cleavage of structural proteins during the assembly of the head of bacteriophage T4 ' (1970 ) 227 Nature : 680 -685 .

    • Search Google Scholar

  • Lee, D. H., Kim, Y. S., Lee, C. B. (2001) The inductive responses of the antioxidant enzymes by salt stress in the rice (Oryza sativa L.). J. Plant Physiol. 158, 737–745.

    Lee C. B. , 'The inductive responses of the antioxidant enzymes by salt stress in the rice (Oryza sativa L.) ' (2001 ) 158 J. Plant Physiol. : 737 -745 .

    • Search Google Scholar

  • Lin, C. C., Kao, C. H. (1996) Proline accumulation is associated with inhibition of rice seedling root growth caused by NaCl. Plant Sci. 114, 121–128.

    Kao C. H. , 'Proline accumulation is associated with inhibition of rice seedling root growth caused by NaCl ' (1996 ) 114 Plant Sci. : 121 -128 .

    • Search Google Scholar

  • Marschner, H. (1995) Mineral Nutrition of Higher Plants. London, Academic Press.

    Marschner H. , '', in Mineral Nutrition of Higher Plants , (1995 ) -.

  • Martía, M. C., Camejoa, D., Fernández-García, N., Rellán-Álvarezb, R., Marquesc, S., Sevilla, F., Jiménez, A. (2009) Effect of oil refinery sludges on the growth and antioxidant system of alfalfa plants. J. Hazard. Mater. 171, 879–885.

    Jiménez A. , 'Effect of oil refinery sludges on the growth and antioxidant system of alfalfa plants ' (2009 ) 171 J. Hazard. Mater. : 879 -885 .

    • Search Google Scholar

  • Moran, J. F., Becana, M., Iturbe-Ormaetxe, I., Frechilla, S., Klucas, R. V., Aparicio-Tejo, P. (1994) Drought induces oxidative stress in pea plants. Planta 194, 346–325.

    Aparicio-Tejo P. , 'Drought induces oxidative stress in pea plants ' (1994 ) 194 Planta : 346 -325 .

    • Search Google Scholar

  • Naya, L., Ladrera, R., Ramos, J., Gonzalez, E. M., Arrese-Igor, C., Minchin, F. R., Becana, M. (2007) The response of carbon metabolism and antioxidant defenses of alfalfa nodules to drought stress and to the subsequent recovery of plants. Plant Physiol. 144, 1104–1114.

    Becana M. , 'The response of carbon metabolism and antioxidant defenses of alfalfa nodules to drought stress and to the subsequent recovery of plants ' (2007 ) 144 Plant Physiol. : 1104 -1114 .

    • Search Google Scholar

  • Noctor, G., Foyer, C. H. (1998) Ascorbate and glutathione: keeping active oxygen under control. Annu. Rev. Plant Physiol. Plant Mol. Biol. 49, 249–279.

    Foyer C. H. , 'Ascorbate and glutathione: keeping active oxygen under control ' (1998 ) 49 Annu. Rev. Plant Physiol. Plant Mol. Biol. : 249 -279 .

    • Search Google Scholar

  • Obinger, C., Maj, M., Nicholls, P., Loewen, P. (1997) Activity, peroxide compound formation, and heme d synthesis in Escherichia coli HPII catalase. Arch. Biochem. Biophys. 342, 58–67.

    Loewen P. , 'Activity, peroxide compound formation, and heme d synthesis in Escherichia coli HPII catalase ' (1997 ) 342 Arch. Biochem. Biophys. : 58 -67 .

    • Search Google Scholar

  • Okeri, H. A., Alonge, P. O. (2006) Determination of the ascorbic acid content of two medicinal plants in Nigeria. Pak. J. Pharm. Sci. 19, 39–44.

    Alonge P. O. , 'Determination of the ascorbic acid content of two medicinal plants in Nigeria ' (2006 ) 19 Pak. J. Pharm. Sci. : 39 -44 .

    • Search Google Scholar

  • Platiša, J., Veljović-Jovanović, S., Kukavica, B., Vinterhalter, B., Smigockic, A., Ninković, S. (2008) Induction of peroxidases and superoxide dismutases in transformed embryogenic calli of alfalfa (Medicago sativa L.). J. Plant Physiol. 165, 895–900.

    Ninković S. , 'Induction of peroxidases and superoxide dismutases in transformed embryogenic calli of alfalfa (Medicago sativa L.) ' (2008 ) 165 J. Plant Physiol. : 895 -900 .

    • Search Google Scholar

  • Poustini, K., Siosemardeh, E. A., Ranjbar, E. M. (2007) Proline accumulation as a response to salt stress in 30 wheat (Triticum aestivum L.) cultivars differing in salt tolerance. Genet. Resour. Crop Evol. 54, 925–934.

    Ranjbar E. M. , 'Proline accumulation as a response to salt stress in 30 wheat (Triticum aestivum L.) cultivars differing in salt tolerance ' (2007 ) 54 Genet. Resour. Crop Evol. : 925 -934 .

    • Search Google Scholar

  • Tambussi, E. A., Bartoli, C. G., Belrano, J., Guiamet, J. J., Arans, J. L. (2000) Oxidative damage to thylakoid protein in water stressed leaves of wheat (Triticum aestivum). Physiol. Plant 108, 398–404.

    Arans J. L. , 'Oxidative damage to thylakoid protein in water stressed leaves of wheat (Triticum aestivum) ' (2000 ) 108 Physiol. Plant : 398 -404 .

    • Search Google Scholar

  • Tejera Garcí, N. A., Iribarne, C., Palma, F., Lluch, C. (2007) Inhibition of the catalase activity from Phaseolus vulgaris and Medicago sativa by sodium chloride. Plant Physiol. Biochem. 45, 535–541.

    Lluch C. , 'Inhibition of the catalase activity from Phaseolus vulgaris and Medicago sativa by sodium chloride ' (2007 ) 45 Plant Physiol. Biochem. : 535 -541 .

    • Search Google Scholar

  • Tennant, D. (1975) A test of modified line intersect method of estimating root length. J. Ecol. 63, 995–1001.

    Tennant D. , 'A test of modified line intersect method of estimating root length ' (1975 ) 63 J. Ecol. : 995 -1001 .

    • Search Google Scholar

  • Vaidyanathan, H., Sivakumar, P., Chakrabarty, R., Thomas, G. (2003) Scavenging of reactive oxygen species in NaCl-stressed rice (Oryza sativa L.)-differential response in salt-tolerant and sensitive varieties. Plant Sci. 165, 1411–1418.

    Thomas G. , 'Scavenging of reactive oxygen species in NaCl-stressed rice (Oryza sativa L.)-differential response in salt-tolerant and sensitive varieties ' (2003 ) 165 Plant Sci. : 1411 -1418 .

    • Search Google Scholar

  • Velikova, V., Yordanov, I., Edreva, A. (2000) Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Protective role of exogenous polyamines. Plant Sci. 151, 59–66.

    Edreva A. , 'Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Protective role of exogenous polyamines ' (2000 ) 151 Plant Sci. : 59 -66 .

    • Search Google Scholar

  • Wang, W. B., Kim, Y. H., Lee, H. S., Kim, K. Y., Deng, X. P., Kwak, S. S. (2009) Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stresses. Plant Physiol. Biochem. 47, 570–577.

    Kwak S. S. , 'Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stresses ' (2009 ) 47 Plant Physiol. Biochem. : 570 -577 .

    • Search Google Scholar

  • Winterbourn, C. C., McGrath, B. W., Carrell, R. W. (1976) Reactions involving superoxide and normal and unstable haemoglobins. Biochem. J. 155, 493–502.

    Carrell R. W. , 'Reactions involving superoxide and normal and unstable haemoglobins ' (1976 ) 155 Biochem. J. : 493 -502 .

    • Search Google Scholar

  • Woodbury, W., Spencer, A. K., Stahman, M. A. (1971) An improved procedure using ferricyanide for detecting catalase isozymes. Anal. Biochem. 44, 301–305.

    Stahman M. A. , 'An improved procedure using ferricyanide for detecting catalase isozymes ' (1971 ) 44 Anal. Biochem. : 301 -305 .

    • Search Google Scholar

  • Zhou, Z. S., Huang, S. Q., Guo, K., Kant Mehta, S., Zhang, P. C., Yang, Z. M. (2007) Metabolical adaptations to mercury-induced oxidative stress in roots of Medicago sativa L. J. Inorg. Biochem. 101, 1–9.

    Yang Z. M. , 'Metabolical adaptations to mercury-induced oxidative stress in roots of Medicago sativa L ' (2007 ) 101 J. Inorg. Biochem. : 1 -9 .

    • Search Google Scholar

  • Zhu, J. K. (2002) Salt and drought stress signal transduction in plants. Annu. Rev. Plant Biol. 53, 247–273.

    Zhu J. K. , 'Salt and drought stress signal transduction in plants ' (2002 ) 53 Annu. Rev. Plant Biol. : 247 -273 .

    • Search Google Scholar
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Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X

Senior editors

Editor(s)-in-Chief: Elekes, Károly

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

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Acta Biologica Hungarica
Language English
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Year of
Foundation		 1950
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Programme		 changed title
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Founder Magyar Tudományos Akadémia
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Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
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ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)

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