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  • 1 University of Szeged Department of Microbiology and Immunobiology, Faculty of Medicine Dóm tér 10 Szeged Hungary
  • 2 University of Szeged Department of Physiology, Faculty of Medicine Dóm tér 10 Szeged Hungary
  • 3 University of Szeged Department of Neurology, Faculty of Medicine Semmelweis utca 6 Szeged Hungary
  • 4 Chelsea and Westminster Hospital Section of Anaesthetics, Pain Medicine and Intensive Care, Department of Surgery and Cancer Faculty of Medicine, Imperial College London London SW10 9NH UK
  • 5 University of Szeged Department of Microbiology, Faculty of Science and Informatics Közép fasor 52 Szeged Hungary
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A new, rapid method is described which permits the genotyping of genetically modified animals from a microlitre volume of whole blood samples via one step polymerase chain reaction amplification. The major advantage of the presented method is the exclusion of a DNA preparation step, which significantly reduces the time expenditure and work load of the genetic testing. Pilot studies indicate, that this method is efficient and applicable also on tissue biopsies and larger amount of blood providing a rapid and reliable new technique over conventional genotyping approaches.

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