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  • 1 Kafrelsheikh University Genetics Department Kafr El-Sheikh 33516 Egypt
  • 2 Kafrelsheikh University Kafr El-Sheikh 33516 Egypt
  • 3 Kafrelsheikh University Horticulture Department, Faculty of Agriculture Kafr El-Sheikh 33516 Egypt
  • 4 Taibah University Department of Applied Biology, Faculty of Applied Science P.O. Box 30002 Almadinah Almunawwarah Saudi Arabia
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Fasciated and normal stem segments of Opuntia microdasys, Opuntia cylindrica, Huernia primulina and Euphorbia lactea were collected from the same plant and compared for their anatomy, water relations and genetic variations. Anatomical differences in terms of thickness of cuticle, vascular bundle, xylem and phloem were analyzed in both normal and fasciated stems. The mucilage cells were higher in the fasciated form of Opuntia microdasys than that in the normal form. Water status in terms of total water content (TWC), water deficit and relative water content (RWC) was influenced by fasciation. Genetic variations were tested in normal and fasciated stems using randomly amplified polymorphic DNA (RAPD) fingerprints and SDS-PAGE of soluble protein extracts. SDS-PAGE protein and RAPD analysis confirmed that normal and fasciated tissues were genetically different. Polymerase chain reaction (PCR) yielded different polymorphic banding patterns that were unique to each primer and distinguishable over all samples. The PCR results of normal and fasciated samples were significantly different in cases of primers P1, P2 and P3. These results indicate that occurrence of fasciation in Opuntia microdasys, Opuntia cylindrica, Huernia primulina and Euphorbia lactea is an epigenetic mutation of tissues.

  • Binggeli, P. (1990) Occurrence and causes of fasciation. Cecidology 5, 57–62.

    Binggeli P , 'Occurrence and causes of fasciation ' (1990 ) 5 Cecidology : 57 -62.

  • Boke, N. H., Ross, R. G. (1978) Fasciation and dichotomous branching in Echinocereus (Cactaceae). Amer. J. Bot. 65, 522–530.

    Ross R G , 'Fasciation and dichotomous branching in Echinocereus (Cactaceae) ' (1978 ) 65 Amer. J. Bot. : 522 -530.

    • Search Google Scholar
  • Bourque, D. P., Pierson, E. (1998) Crested saguaros: What is the rhyme or reason?. Plant Mol. Biol. Rep. 16, 217–218.

    Pierson E , 'Crested saguaros: What is the rhyme or reason? ' (1998 ) 16 Plant Mol. Biol. Rep. : 217 -218.

    • Search Google Scholar
  • Clark, S. E., Running, M. P., Meyerowitz, E. M. (1993) CLAVATA1, a regulator of meristem and flower development in Arabidopsis. Development 119, 397–418.

    Meyerowitz E M , 'CLAVATA1, a regulator of meristem and flower development in Arabidopsis ' (1993 ) 119 Development : 397 -418.

    • Search Google Scholar
  • Dirr, M. (1998) Manual of Woody Landscape Plants: Their Identification, Ornamental Characteristics, Culture, Propagation and Uses. 5th ed. Stipes Publishing LLC, Champaign.

    Dirr M , '', in Manual of Woody Landscape Plants: Their Identification, Ornamental Characteristics, Culture, Propagation and Uses , (1998 ) -.

    • Search Google Scholar
  • Doyle, J. J., Doyle, J. L. (1990) Isolation of plant DNA from fresh tissue. Focus 12, 13–15.

    Doyle J L , 'Isolation of plant DNA from fresh tissue ' (1990 ) 12 Focus : 13 -15.

  • Driss-Ecole, D. (1981) Fasciation from excised shoot apices in Celosia cristata (Amaranthaceae) cultivated in vitro. Can. J. Bot. 59, 1367–1373.

    Driss-Ecole D , 'Fasciation from excised shoot apices in Celosia cristata (Amaranthaceae) cultivated in vitro ' (1981 ) 59 Can. J. Bot. : 1367 -1373.

    • Search Google Scholar
  • El-Banna, A., Shalaby, T. (2012) Detection of mutations in tomato induced by EMS using RAPD and SSR markers. Ann. Agric. Sci. 1, 21–28.

    Shalaby T , 'Detection of mutations in tomato induced by EMS using RAPD and SSR markers ' (2012 ) 1 Ann. Agric. Sci. : 21 -28.

    • Search Google Scholar
  • Hall, A. E. (2001) Crop Responses to Environments. CRC Press, Boca Raton, FL, USA.

    Hall A E , '', in Crop Responses to Environments , (2001 ) -.

  • Gyulai, G., J. A. Gémesné, Zs. Sági, G. Venczel, P. Pintér, Z. Kristóf, O. Törjék, L. Heszky, S. Bottka, J. Kiss, L. Zatykó (2000) Doubled haploid development and PCR-analysis of F1 hybrid derived DH-R2 paprika (Capsicum annuum L.) lines. J. Plant Physiol. 156, 168–174.

    Zatykó L , 'Doubled haploid development and PCR-analysis of F1 hybrid derived DH-R2 paprika (Capsicum annuum L.) lines ' (2000 ) 156 J. Plant Physiol. : 168 -174.

    • Search Google Scholar
  • Iliev, I., Kitin, P. (2011) Origin, morphology, and anatomy of fasciationin plants cultured in vivo and in vitro. Plant Growth Regulation 63, 115–129.

    Kitin P , 'Origin, morphology, and anatomy of fasciationin plants cultured in vivo and in vitro ' (2011 ) 63 Plant Growth Regulation : 115 -129.

    • Search Google Scholar
  • Kalapos, T. (1994) Leaf water potential and leaf water deficit relationship for ten species of semiarid grassland community. Plant Soil. 160, 105–112.

    Kalapos T , 'Leaf water potential and leaf water deficit relationship for ten species of semiarid grassland community ' (1994 ) 160 Plant Soil. : 105 -112.

    • Search Google Scholar
  • Kamikouchi, A., Morioka, M., Kubo, T. (2004) Identification of honeybee antennal proteins/genes expressed in a sex- and/or caste selective manner. Zool. Sci. 21, 53–62.

    Kubo T , 'Identification of honeybee antennal proteins/genes expressed in a sex- and/or caste selective manner ' (2004 ) 21 Zool. Sci. : 53 -62.

    • Search Google Scholar
  • Kitamoto, N., Nakamoto, H., Katai, A., Takahara, N., Nakata, H., Tamaki, H., Tanaka, T. (1998) Heterogeneity of protein profiles of Helicobacter pylori isolated from individual patients. Helicobacter 3, 152–162.

    Tanaka T , 'Heterogeneity of protein profiles of Helicobacter pylori isolated from individual patients ' (1998 ) 3 Helicobacter : 152 -162.

    • Search Google Scholar
  • Kitin, P., Iliev, I., Scaltsoyiannes, A., Nellas, C., Rubos, A., Funada, R. (2005) A comparative histological study between normal and fasciated shoots of Prunus avium generated in vitro. Plant Cell Tiss. Org. Cult. 82, 141–150.

    Funada R , 'A comparative histological study between normal and fasciated shoots of Prunus avium generated in vitro ' (2005 ) 82 Plant Cell Tiss. Org. Cult. : 141 -150.

    • Search Google Scholar
  • Krusmann, G. (1995) Manual of Cultivated Conifers. Ed. H.-D. Warda. Timber Press, Portland, Oregon.

  • Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.

    Laemmli U K , 'Cleavage of structural proteins during the assembly of the head of bacteriophage T4 ' (1970 ) 227 Nature : 680 -685.

    • Search Google Scholar
  • Lambers, H., Chapin, F. S., Pons, T. L. (1998) Plant Physiological Ecology. Springer-Verlag, New York, NY, USA.

    Pons T L , '', in Plant Physiological Ecology , (1998 ) -.

  • LaMotte, C. E., Curry, T. M., Palmer, R. G., Albertsen, M. C. (1988) Developmental anatomy and morphology of fasciation in the soybean (Glycine max). Bot. Gaz. 149, 398–407.

    Albertsen M C , 'Developmental anatomy and morphology of fasciation in the soybean (Glycine max) ' (1988 ) 149 Bot. Gaz. : 398 -407.

    • Search Google Scholar
  • Lawlor, D. W., Cornic, G. (2002) Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants. Plant Cell Environ. 25, 275–294.

    Cornic G , 'Photosynthetic carbon assimilation and associated metabolism in relation to water deficits in higher plants ' (2002 ) 25 Plant Cell Environ. : 275 -294.

    • Search Google Scholar
  • Lortal, S., Valence. F., Bizet, C., Maubois, J. L. (1997) Electrophotic pattern of peptidoglycan hydrolases, a new tool for bacterial species identification: application to 10 Lactobacillus species. Res. Microbiol. 148, 461–474.

    Maubois J L , 'Electrophotic pattern of peptidoglycan hydrolases, a new tool for bacterial species identification: application to 10 Lactobacillus species ' (1997 ) 148 Res. Microbiol. : 461 -474.

    • Search Google Scholar
  • Masters, M. T. (1886) Pflanzen-Teratologie. Verlag von H. Haessel, Leipzig, pp. 25–37.

    Masters M T , '', in Pflanzen-Teratologie , (1886 ) -.

  • Mitras, D., Kitin, P., Iliev, I., Dancheva, D., Scaltsoyiannes, A., Tsaktsira, M., Nellas, C., Rohr, R. (2009) In vitro propagation of Fraxinus excelsior L. by epicotyls. J. Biol. Res. 11, 37–48.

    Rohr R , 'In vitro propagation of Fraxinus excelsior L. by epicotyls ' (2009 ) 11 J. Biol. Res. : 37 -48.

    • Search Google Scholar
  • Nilsson, O., Moritz, T., Sundberg, B., Sandberg, G., Olsson, O. (1996) Expression of the Agrobacterium rhizogenes rolC gene in a deciduous forest tree alters growth and development and leads to stem fasciation. J. Plant Physiol. 112, 493–502.

    Olsson O , 'Expression of the Agrobacterium rhizogenes rolC gene in a deciduous forest tree alters growth and development and leads to stem fasciation ' (1996 ) 112 J. Plant Physiol. : 493 -502.

    • Search Google Scholar
  • Papafotiou, M., Balotis, G., Louka, P., Chronopoulos, J. (2001) In vitro plant regeneration of Mammillaria elongata normal and cristate forms. Plant Cell Tiss. Org. Cult. 65, 163–167.

    Chronopoulos J , 'In vitro plant regeneration of Mammillaria elongata normal and cristate forms ' (2001 ) 65 Plant Cell Tiss. Org. Cult. : 163 -167.

    • Search Google Scholar
  • Rani, V., Parida, A., Raina, S. N. (1995) Random amplified polymorphic DNA (RAPD) markers for genetic analysis in micropropagated plants of Populus deltoids Marsh. Plant Cell Rep. 14, 459–462.

    Raina S N , 'Random amplified polymorphic DNA (RAPD) markers for genetic analysis in micropropagated plants of Populus deltoids Marsh ' (1995 ) 14 Plant Cell Rep. : 459 -462.

    • Search Google Scholar
  • Raimondi, J. P., Masuelli, R. W., Camadro, E. L. (2001) Assessment of somaclonal variation in asparagus by RAPD fingerprinting and cytogenetic analyses. Sci. Hortic.-Amsterdam 90, 19–29.

    Camadro E L , 'Assessment of somaclonal variation in asparagus by RAPD fingerprinting and cytogenetic analyses ' (2001 ) 90 Sci. Hortic.-Amsterdam : 19 -29.

    • Search Google Scholar
  • Ruibal-Mendieta, N. L., Lints, F. (1998) Novel and transgenic food crops: Overview of scientific versus public perception. Transgenic Res. 7, 379–386.

    Lints F , 'Novel and transgenic food crops: Overview of scientific versus public perception ' (1998 ) 7 Transgenic Res. : 379 -386.

    • Search Google Scholar
  • Ruzin, S. E. (1999) Plant Microtechniques and Microscopy, First Ed. Oxford University Press, USA.

    Ruzin S E , '', in Plant Microtechniques and Microscopy , (1999 ) -.

  • Sinjushin, A. A., Gostimsky, S. A. (2006) Fasciation in pea: basic principles of morphogenesis. Russ. J. Dev. Biol. 37, 375–381.

    Gostimsky S A , 'Fasciation in pea: basic principles of morphogenesis ' (2006 ) 37 Russ. J. Dev. Biol. : 375 -381.

    • Search Google Scholar
  • Sinjushin, A. A., Gostimsky, S. A. (2008) Genetic control of fasciation in pea (Pisum sativum L.). Russ. J. Genet. 44, 807–814.

    Gostimsky S A , 'Genetic control of fasciation in pea (Pisum sativum L.) ' (2008 ) 44 Russ. J. Genet. : 807 -814.

    • Search Google Scholar
  • Snyder, E. E., Weber, D. J. (1966) Causative factors of cristation in the Cactaceae. Cactus and Succulent J. 38, 27–32.

    Weber D J , 'Causative factors of cristation in the Cactaceae ' (1966 ) 38 Cactus and Succulent J. : 27 -32.

    • Search Google Scholar
  • Studier, F. W. (1973) Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J. Mol. Biol. 79, 237–248.

    Studier F W , 'Analysis of bacteriophage T7 early RNAs and proteins on slab gels ' (1973 ) 79 J. Mol. Biol. : 237 -248.

    • Search Google Scholar
  • Tang, Y., Knap, H. T. (1998) Fasciation mutation enhances meristematic activity and alters pattern formation in soybean. Int. J. Plant Sci. 159, 249–260.

    Knap H T , 'Fasciation mutation enhances meristematic activity and alters pattern formation in soybean ' (1998 ) 159 Int. J. Plant Sci. : 249 -260.

    • Search Google Scholar
  • Vallicelli, V. (2010) Cactuspedia. http://www.cactuspedia.info/ . Accessed 20 May 2010

    Vallicelli V , '', in Cactuspedia , (2010 ) -.

  • Van Gelderen, D. M., Van Hoey Smith, J. R. P. (1997) Conifers: The Illustrated Encyclopedia. Vols 1 and 2. Timber Press, Portland.

    Hoey Smith J R P , '', in Conifers: The Illustrated Encyclopedia , (1997 ) -.

  • Weatherley, P. E. (1950) Studies in the water relations of the cotton plant. I. The field measurement of water deficits in leaves. New Phytologist 49, 81–87.

    Weatherley P E , 'Studies in the water relations of the cotton plant. I. The field measurement of water deficits in leaves ' (1950 ) 49 New Phytologist : 81 -87.

    • Search Google Scholar
  • Werner, R. (1988) Unusual growth in succulent Euphorbias. ‘Fasciation & Dichotomus Branching’. The Euphorbia Journal 5, 7–17.

    Werner R , 'Unusual growth in succulent Euphorbias. ‘Fasciation & Dichotomus Branching’ ' (1988 ) 5 The Euphorbia Journal : 7 -17.

    • Search Google Scholar
  • Williams, J. G. K., Kublik, A. R., Livak, K. J., Rafalski, J. A., Ingey, S. V. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18, 6531–6535.

    Ingey S V , 'DNA polymorphisms amplified by arbitrary primers are useful as genetic markers ' (1990 ) 18 Nucleic Acids Res. : 6531 -6535.

    • Search Google Scholar
  • Yadav, K., Kumar, S., Yadav, A., Yadav, V., Pandey, P., Nath Dwivedi, U. (2012) Genetic Diversity of Pigeonpea (Cajanus cajan (L.) Millsp.) cultivars and its wild relatives using randomly amplified polymorphic DNA (RAPD) markers. Am. J. Plant Sci. 3, 322–330.

    Nath Dwivedi U , 'Genetic Diversity of Pigeonpea (Cajanus cajan (L.) Millsp.) cultivars and its wild relatives using randomly amplified polymorphic DNA (RAPD) markers ' (2012 ) 3 Am. J. Plant Sci. : 322 -330.

    • Search Google Scholar