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  • 1 Thepsatri Rajabhat University Department of Biology, Faculty of Science and Technology Maung, Lopburi 15000 Thailand
  • 2 Szent István University Molecular Animal Biotechnology Laboratory Gödöllő H-2100 Hungary
  • 3 Mahidol University Department of Anatomy, Faculty of Science Rama VI Road Bangkok 10400 Thailand
  • 4 Mahidol University Thalassemia Research Centre, Institute of Molecular Biosciences Salaya, Nakornpathom 73170 Thailand
  • 5 Mahidol University Department of Biochemistry, Faculty of Science Rama VI Road Bangkok 10400 Thailand
  • 6 Mahidol University Reproductive and Stem cell Biology Research group, Institute of Molecular Biosciences Salaya, Nakornpathom 73170 Thailand
  • 7 The University of Melbourne, Royal Children’s Hospital Cell and Gene Therapy Research Group, The Murdoch Children’s Research Institute Flemington Road, Parkville 3052 Victoria Australia
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A major clinical feature of patients with thalassemia is growth retardation due to anemia, therefore, the hematological parameters, weanling weight and post-weanling weight of pups obtained from vitrifiedwarmed embryo transfers were studied for the first time in this report. Two-cell embryos of four transgenic (TG) thalassemic mouse lines (BKO, 654, E2, and E4) were produced by breeding four lines of TG thalassemic males to wild-type (WT) females (C57BL/6J) and were cryopreserved by vitrification in straws using 35% ethylene glycol. After transfer of vitrified-warmed embryos, hematological parameters, spleen index, weanling and post-weanling weight were determined in TG and WT viable pups. In the BKO and 654 mice significantly abnormal hematological parameters and spleen index were observed compared to WT, E2 and E4 mice. The weanling and post-weanling weights of BKO and 654 pups were significantly less than that of the age-matched WT pups. However, no significant differences in weanling and post-weanling weight were found between WT and E2-TG or E4-TG pups. In conclusion, the four transgenic mice lines produced from cryopreserved embryo transfer retain the phenotype of the natural breeding mice indicating that these banked embryos are appropriate for thalassemia model productions.

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