Authors:
Z. Bali University of Pécs Department of Experimental Zoology and Neurobiology, and Szentágothai Research Center Pécs Hungary

Search for other papers by Z. Bali in
Current site
Google Scholar
PubMed
Close
,
D. Budai Kation Scientific, LLC Minneapolis MN USA

Search for other papers by D. Budai in
Current site
Google Scholar
PubMed
Close
, and
I. Hernádi University of Pécs Department of Experimental Zoology and Neurobiology, and Szentágothai Research Center Pécs Hungary

Search for other papers by I. Hernádi in
Current site
Google Scholar
PubMed
Close
Restricted access

Microiontophoresis combined with extracellular spike recording is an excellent method for investigating local neuropharmacological effects under in vivo conditions. However, its application has recently become relatively rare in neuroscience research. Now, we aimed to revisit microiontophoresis and demonstrate that it provides valuable data about the pharmacophysiology of neurons and local neuronal networks, in vivo. Extracellular recordings were performed through the central recording channel of multibarrel carbon-fiber microelectrodes in the CA1 pyramidal layer of the hippocampus of anesthetized rats, while N-methyl-D-aspartate (NMDA) was locally administrated by means of microiontophoresis through the surrounding micropipettes of the microelectrode. Various separation procedures were used to distinguish putative pyramidal cells and interneurons. Quality of separation was verified by electrophysiological parameters. After the delivery of NMDA in the vicinity of the examined neurons, firing rate of putative pyramidal cells was increased with a significantly higher grade then that of putative interneurons. The present results in line with previous data indicate that pyramidal cells are more responsive to pharmacological manipulation through NMDA receptors, also confirming the reliability of the separation of different types of neurons in in vivo microiontophoretic experiments.

  • Alkondon, M., Albuquerque, E. X. (2012) Nicotinic acetylcholine receptor α7 and α4β2 subtypes differentially control GABAergic input to CA1 neurons in rat hippocampus. J. Neurophysiol. 3043–3055.

    Albuquerque E. X. , '', in J. Neurophysiol. , (2012 ) -.

  • Avignone, E., Frenguelli, B. G., Irving, A. J. (2005) Differential responses to NMDA receptor activation in rat hippocampal interneurons and pyramidal cells may underlie enhanced pyramidal cell vulnerability. Eur. J. Neurosci. 22, 3077–3090.

    Irving A. J. , 'Differential responses to NMDA receptor activation in rat hippocampal interneurons and pyramidal cells may underlie enhanced pyramidal cell vulnerability ' (2005 ) 22 Eur. J. Neurosci. : 3077 -3090 .

    • Search Google Scholar
  • Bird, S. J., Aghajanian, G. K. (1976) The cholinergic pharmacology of hippocampal pyramidal cells: a microiontophoretic study. Neuropharmacology 15, 273–282.

    Aghajanian G. K. , 'The cholinergic pharmacology of hippocampal pyramidal cells: a microiontophoretic study ' (1976 ) 15 Neuropharmacology : 273 -282 .

    • Search Google Scholar
  • Boakes, R. J., Bramwell, G. J., Briggs, I., Candy, J. M., Tempesta, E. (1974) Localization with Pontamine Sky Blue of neurones in the brainstem responding to microiontophoretically applied compounds. Neuropharmacology 13, 475–479.

    Tempesta E. , 'Localization with Pontamine Sky Blue of neurones in the brainstem responding to microiontophoretically applied compounds ' (1974 ) 13 Neuropharmacology : 475 -479 .

    • Search Google Scholar
  • Budai, D. (2010) Carbon fiber-based microelectrodes and microbiosensors. In: Somerset, V. S. (ed.) Intelligent and Biosensors. InTech, Europa, Rijeka, pp. 269–288. Also available online for open access: http://www.intechopen.com/books/intelligent-and-biosensors

    Budai D. , '', in Intelligent and Biosensors. , (2010 ) -.

  • Csicsvári, J., Hirase, H., Czurko, A., Buzsáki, G. (1998) Reliability and state dependence of pyramidal cell-interneuron synapses in the hippocampus: an ensemble approach in the behaving rat. Neuron 21, 179–189.

    Buzsáki G. , 'Reliability and state dependence of pyramidal cell-interneuron synapses in the hippocampus: an ensemble approach in the behaving rat ' (1998 ) 21 Neuron : 179 -189 .

    • Search Google Scholar
  • Czéh, B., Ábrahám, H., Tahtakran, S., Houser, C. R., Seress, L. (2013) Number and regional distribution of GAD65 mRNA-expressing interneurons in the rat hippocampal formation. Acta Biol. Hung. 64, 395–413.

    Seress L. , 'Number and regional distribution of GAD65 mRNA-expressing interneurons in the rat hippocampal formation ' (2013 ) 64 Acta Biol. Hung. : 395 -413 .

    • Search Google Scholar
  • Del Castillo, J., Katz, B. (1955) On the localization of acetylcholine receptors. J. Physiol. 128, 157–181.

    Katz B. , 'On the localization of acetylcholine receptors ' (1955 ) 128 J. Physiol. : 157 -181 .

    • Search Google Scholar
  • Fox, S. E., Ranck, J. B. (1981) Electrophysiological characteristics of hippocampal complex-spike cells and theta cells. Exp. Brain Res. 41, 399–410.

    Ranck J. B. , 'Electrophysiological characteristics of hippocampal complex-spike cells and theta cells ' (1981 ) 41 Exp. Brain Res. : 399 -410 .

    • Search Google Scholar
  • Furue, H., Katafuchi, T., Yoshimura, M. (2007) In vivo patch-clamp technique. In: Walz, W. (ed.) Patch-Clamp Analysis. Humana Press, pp. 229–251.

    Yoshimura M. , '', in Patch-Clamp Analysis , (2007 ) -.

  • Gerhardt, G. A., Palmer, M. R. (1987) Characterization of the techniques of pressure ejection and microiontophoresis using in vivo electrochemistry. J. Neurosci. Methods 22, 147–159.

    Palmer M. R. , 'Characterization of the techniques of pressure ejection and microiontophoresis using in vivo electrochemistry ' (1987 ) 22 J. Neurosci. Methods : 147 -159 .

    • Search Google Scholar
  • Harris, K. D., Hirase, H., Leinekugel, X., Henze, D. A., Buzsáki, G. (2001) Temporal interaction between single spikes and complex spike bursts in hippocampal pyramidal cells. Neuron 32, 141–149.

    Buzsáki G. , 'Temporal interaction between single spikes and complex spike bursts in hippocampal pyramidal cells ' (2001 ) 32 Neuron : 141 -149 .

    • Search Google Scholar
  • Hazan, L., Zugaro, M., Buzsáki, G. (2006) Klusters, NeuroScope, NDManager: a free software suite for neurophysiological data processing and visualization. J. Neurosci. Methods 155, 207–216.

    Buzsáki G. , 'Klusters, NeuroScope, NDManager: a free software suite for neurophysiological data processing and visualization ' (2006 ) 155 J. Neurosci. Methods : 207 -216 .

    • Search Google Scholar
  • Joëls, M., Urban, I. J. (1982) The effect of microiontophoretically applied vasopressin and oxytocin on single neurones in the septum and dorsal hippocampus of the rat. Neurosci. Lett. 33, 79–84.

    Urban I. J. , 'The effect of microiontophoretically applied vasopressin and oxytocin on single neurones in the septum and dorsal hippocampus of the rat ' (1982 ) 33 Neurosci. Lett. : 79 -84 .

    • Search Google Scholar
  • Klausberger, T., Somogyi, P. (2008) Neuronal diversity and temporal dynamics: the unity of hippocampal circuit operations. Science 321, 53–57. 21.

    Somogyi P. , 'Neuronal diversity and temporal dynamics: the unity of hippocampal circuit operations ' (2008 ) 321 Science : 53 -57 .

    • Search Google Scholar
  • Kovács, P., Hernádi, I. (2006) Yohimbine acts as a putative in vivo alpha2A/D-antagonist in the rat prefrontal cortex. Neurosci. Lett. 402, 253–258.

    Hernádi I. , 'Yohimbine acts as a putative in vivo alpha2A/D-antagonist in the rat prefrontal cortex ' (2006 ) 402 Neurosci. Lett. : 253 -258 .

    • Search Google Scholar
  • Kovács, P., Dénes, V., Kellényi, L., Hernádi, I. (2005) Microiontophoresis electrode location by neurohistological marking: Comparison of four native dyes applied from current balancing electrode channels. J. Pharmacol. Toxicol. Methods 51, 147–151.

    Hernádi I. , 'Microiontophoresis electrode location by neurohistological marking: Comparison of four native dyes applied from current balancing electrode channels ' (2005 ) 51 J. Pharmacol. Toxicol. Methods : 147 -151 .

    • Search Google Scholar
  • LacKamp, A., Zhang, G.-C., Mao, L.-M., Fibuch, E. E., Wang, J. Q. (2009) Loss of surface N-methyl-D-aspartate receptor proteins in mouse cortical neurones during anaesthesia induced by chloral hydrate in vivo. Br. J. Anaesth. 102, 515–522.

    Wang J. Q. , 'Loss of surface N-methyl-D-aspartate receptor proteins in mouse cortical neurones during anaesthesia induced by chloral hydrate in vivo ' (2009 ) 102 Br. J. Anaesth. : 515 -522 .

    • Search Google Scholar
  • Martina, M., Comas, T., Mealing, G. A. R. (2013) Selective pharmacological modulation of pyramidal neurons and interneurons in the CA1 region of the rat hippocampus. Front. Pharmacol. 4, 24.

    Mealing G. A. R. , 'Selective pharmacological modulation of pyramidal neurons and interneurons in the CA1 region of the rat hippocampus ' (2013 ) 4 Front. Pharmacol. : 24 -.

    • Search Google Scholar
  • Mizuseki, K., Sirota, A., Pastalkova, E., Buzsáki, G. (2009) Theta oscillations provide temporal windows for local circuit computation in the entorhinal-hippocampal loop. Neuron 64, 267–280.

    Buzsáki G. , 'Theta oscillations provide temporal windows for local circuit computation in the entorhinal-hippocampal loop ' (2009 ) 64 Neuron : 267 -280 .

    • Search Google Scholar
  • Nyíri, G., Stephenson, F., Freund, T., Somogyi, P. (2003) Large variability in synaptic n-methyl-d-aspartate receptor density on interneurons and a comparison with pyramidal-cell spines in the rat hippocampus. Neuroscience 119, 347–363.

    Somogyi P. , 'Large variability in synaptic n-methyl-d-aspartate receptor density on interneurons and a comparison with pyramidal-cell spines in the rat hippocampus ' (2003 ) 119 Neuroscience : 347 -363 .

    • Search Google Scholar
  • Pang, K., Rose, G. M. (1987) Differential effects of norepinephrine on hippocampal complex-spike and theta-neurons. Brain Res. 425, 146–158.

    Rose G. M. , 'Differential effects of norepinephrine on hippocampal complex-spike and theta-neurons ' (1987 ) 425 Brain Res. : 146 -158 .

    • Search Google Scholar
  • Paxinos, G., Watson, C. (1997) The rat brain in stereotaxic coordinates, 3rd Ed. Academic Press, London.

    Watson C. , '', in The rat brain in stereotaxic coordinates , (1997 ) -.

  • Pinault, D. (1996) A novel single-cell staining procedure performed in vivo under electrophysiological control: morpho-functional features of juxtacellularly labeled thalamic cells and other central neurons with biocytin or neurobiotin. J. Neurosci. Meth. 65, 113–136.

    Pinault D. , 'A novel single-cell staining procedure performed in vivo under electrophysiological control: morpho-functional features of juxtacellularly labeled thalamic cells and other central neurons with biocytin or neurobiotin ' (1996 ) 65 J. Neurosci. Meth. : 113 -136 .

    • Search Google Scholar
  • Ranck, J. B. (1973) Studies on single neurons in dorsal hippocampal formation and septum in unrestrained rats. I. Behavioral correlates and firing repertoires. Exp. Neurol. 41, 461–531.

    Ranck J. B. , 'Studies on single neurons in dorsal hippocampal formation and septum in unrestrained rats. I. Behavioral correlates and firing repertoires ' (1973 ) 41 Exp. Neurol. : 461 -531 .

    • Search Google Scholar
  • Sah, P., Hestrin, S., Nicoll, R. A. (1990) Properties of excitatory postsynaptic currents recorded in vitro from rat hippocampal interneurones. J. Physiol. 430, 605–616.

    Nicoll R. A. , 'Properties of excitatory postsynaptic currents recorded in vitro from rat hippocampal interneurones ' (1990 ) 430 J. Physiol. : 605 -616 .

    • Search Google Scholar
  • Segal, M., Greenberger, V. (1992) Acidic amino acids evoke a smaller [Ca2+]i rise in GABAergic than non-GABAergic hippocampal neurons. Neurosci. Lett. 140, 243–246.

    Greenberger V. , 'Acidic amino acids evoke a smaller [Ca2+]i rise in GABAergic than non-GABAergic hippocampal neurons ' (1992 ) 140 Neurosci. Lett. : 243 -246 .

    • Search Google Scholar
  • Szegedi, V., Juhász, G., Parsons, C. G., Budai, D. (2010) In vivo evidence for functional NMDA receptor blockade by memantine in rat hippocampal neurons. J. Neural. Transm. 117, 1189–1194.

    Budai D. , 'In vivo evidence for functional NMDA receptor blockade by memantine in rat hippocampal neurons ' (2010 ) 117 J. Neural. Transm. : 1189 -1194 .

    • Search Google Scholar
  • Vida, I., Frotscher, M. (2000) A hippocampal interneuron associated with the mossy fiber system. Proc. Natl Acad. Sci. USA 97, 1275–1280.

    Frotscher M. , 'A hippocampal interneuron associated with the mossy fiber system ' (2000 ) 97 Proc. Natl Acad. Sci. USA : 1275 -1280 .

    • Search Google Scholar
  • West, M. J., Coleman, P. D., Flood, D. G., Troncoso, J. C. (1994) Differences in the pattern of hippocampal neuronal loss in normal ageing and Alzheimer’s disease. Lancet 344, 769–772.

    Troncoso J. C. , 'Differences in the pattern of hippocampal neuronal loss in normal ageing and Alzheimer’s disease ' (1994 ) 344 Lancet : 769 -772 .

    • Search Google Scholar
  • Collapse
  • Expand

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

Acta Biologica Hungarica
P.O. Box 35
H-8237 Tihany, Hungary
Phone: (36 87) 448 244 ext. 103
Fax: (36 87) 448 006
E-mail: elekes@tres.blki.hu

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture
  • Biology and Environmental Sciences
  • Elsevier BIOBASE
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Acta Biologica Hungarica
Language English
Size  
Year of
Foundation
1950
Publication
Programme
changed title
Volumes
per Year
 
Issues
per Year
 
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)