Acta Biologica Hungarica
Volume/Issue:
Volume 65: Issue 3
Effects of environmental factors on sperm motility and fertilization rate of Phascolosoma esculenta
Authors: Z. Sheng 1 , J.-H. Jiang, C.-H. Jin 1 , S.-J. Xu 1 , and J.-Q. Zhu 1
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  • 1 Ningbo University School of Marine Sciences Ningbo, Zhejiang 315211 China
  • 2 Zhejiang Institute of Freshwater Fisheries Huzhou, Zhejiang 313001 China
DOI:
https://doi.org/10.1556/abiol.65.2014.3.4
Page Count:
274–284
Publication Date:
01 Sep 2014
Online Publication Date:
06 Sep 2014
Article Category:
Research Article
Restricted access

The study investigated the effects of environmental factors (salinity, pH, ions and activation media) on sperm motility (activation rate, duration of quick movement, and lifespan) and fertilization rate of Phascolosoma esculenta. The results showed that spermatozoa in the coelom and nephridium are able to move quickly. The optimal salinity was 14.64 to 43.35 and the optimal pH was 6.46 to 9.53 for sperm activation and motility, whereas the ranges for fertilization were narrower (18.56 to 30.3 for salinity and 6.46 to 8.61 for pH). Of the ions studied, Na+ was indispensable for sperm motility and fertilization, and Ca2+ and Mg2+ were necessary for fertilization. P. esculenta sperm could not fertilize eggs and have short lifespans in 200 to 600 mmol/L NaCl and KCl solutions. Furthermore, they could not be activated or move in 200 to 600 mmol/L CaCl2, MgSO4, and sucrose solutions.

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Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X
Keywords:
Phascolosoma esculenta ; sperm motility; fertilization rate; environmental factors

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