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  • 1 Adiyaman University Department of Biology, Art and Science Faculty Adiyaman Turkey
  • 2 Adiyaman University Department of Plant and Animal Production, Kahta Vocational School Adiyaman Turkey
  • 3 Adiyaman University Graduate School of Sciences Adiyaman Turkey
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In this study, the changes caused by chilling stress on some physiological parameters of pepino (Solanum muricatum Ait.) plant and the effects of ascorbic acid (100 mM) applied exogenously on these changes were examined. For this purpose, the photosynthetic pigments (chlorophyll a, chlorophyll b, total chlorophylls and carotenoids), ascorbic acid, total phenolic compounds, malondialdehyde and proline contents in leaves of pepino taken on 5th and 10th days were determined. As a result of chilling stress, it was found that while the photosynthetic pigments and proline contents decreased in pepino leaves, the ascorbic acid, total phenolic compounds and malondialdehyde contents increased. In plants which were subjected to pre-treatment of ascorbic acid on the 10th day of stress, ascorbic acid and proline contents increased while a decrease was observed in malondialdehyde content, compared to stress group without pre-treated. This study may be important for explaining resistance induced by treatment of exogenous ascorbic acid in pepino exposed to chilling stress.

  • Ahumada, M., Cantwell, M. (1996) Postharvest studies on pepino dulce (Solanum muricatum Ait.): maturity at harvest and storage behavior. Postharvest Biol. Tec. 7, 129–136.

    Cantwell M. , 'Postharvest studies on pepino dulce (Solanum muricatum Ait.): maturity at harvest and storage behavior ' (1996 ) 7 Postharvest Biol. Tec. : 129 -136.

    • Search Google Scholar
  • Bates, L. S., Waldren, R. P., Teare, I. D. (1973) Rapid determination of proline for water-studies. Plant Soil. 39, 205–207.

    Teare I. D. , 'Rapid determination of proline for water-studies ' (1973 ) 39 Plant Soil. : 205 -207.

    • Search Google Scholar
  • Bravo, M. A., Arias, E. (1983) Cultivo del pepino dulce. antecedentes agronómicos y económicos. El Campesino. 114(3), 15–34.

    Arias E. , 'Cultivo del pepino dulce. antecedentes agronómicos y económicos ' (1983 ) 114 El Campesino. : 15 -34.

    • Search Google Scholar
  • Bybordi, A. (2012) Effect of ascorbic acid and silicium on photosynthesis, antioxidant enzyme activity, and fatty acid contents in canola exposure to salt stress. J. Integrative Agri. 11, 1610–1620.

    Bybordi A. , 'Effect of ascorbic acid and silicium on photosynthesis, antioxidant enzyme activity, and fatty acid contents in canola exposure to salt stress ' (2012 ) 11 J. Integrative Agri. : 1610 -1620.

    • Search Google Scholar
  • Cao, S., Cai, Y., Yang, Z., Zheng, Y. (2012) MeJA induces chilling tolerance in loquat fruit by regulating proline and γ-aminobutyric acid contents. Food Chem. 133, 1466–1470.

    Zheng Y. , 'MeJA induces chilling tolerance in loquat fruit by regulating proline and γ-aminobutyric acid contents ' (2012 ) 133 Food Chem. : 1466 -1470.

    • Search Google Scholar
  • Chandler, S. F., Dodds, J. H. (1983) The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasidine in callus cultures of Solanum lacinitum. Plant Cell Rep. 2, 105–110.

    Dodds J. H. , 'The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasidine in callus cultures of Solanum lacinitum ' (1983 ) 2 Plant Cell Rep. : 105 -110.

    • Search Google Scholar
  • De Kok, L. J., Graham, M. (1989) Levels of pigments, soluble proteins, amino acids and sulfhydryl compounds in foliar tissue of Arabidopsis thaliana during dark-induced and natural senesence. Plant Physiol. Biochem. 27, 203–209.

    Graham M. , 'Levels of pigments, soluble proteins, amino acids and sulfhydryl compounds in foliar tissue of Arabidopsis thaliana during dark-induced and natural senesence ' (1989 ) 27 Plant Physiol. Biochem. : 203 -209.

    • Search Google Scholar
  • Delauney, A. J., Verma, D. P. S. (1993) Proline biosynthesis and osmoregulation in plants. Plant J. 4, 215–223.

    Verma D. P. S. , 'Proline biosynthesis and osmoregulation in plants ' (1993 ) 4 Plant J. : 215 -223.

    • Search Google Scholar
  • Erdal, S. (2012) Androsterone-induced molecular and physiological changes in maize seedlings in response to chilling stress. Plant Physiol. Biochem. 57, 1–7.

    Erdal S. , 'Androsterone-induced molecular and physiological changes in maize seedlings in response to chilling stress ' (2012 ) 57 Plant Physiol. Biochem. : 1 -7.

    • Search Google Scholar
  • Foyer, C. H., Vanacker, H., Goméz, L. D., Harbinson, J. (2002) Regulation of photosynthesis and antioxidant metabolism in maize leaves at optimal and chilling temperatures: review. Plant Physiol. Biochem. 40, 659–668.

    Harbinson J. , 'Regulation of photosynthesis and antioxidant metabolism in maize leaves at optimal and chilling temperatures: review ' (2002 ) 40 Plant Physiol. Biochem. : 659 -668.

    • Search Google Scholar
  • Fujita, M., Fujita, Y., Noutoshi, Y., Takahashi, F., Narusaka, Y., Yamaguchi-Shinozaki, K., Shinozaki, K. (2006) Crosstalk between abiotic and biotic stress responses: a current view from the points of convergence in the stress signaling Networks. Curr. Opin. Plant Biol. 9, 436–442.

    Shinozaki K. , 'Crosstalk between abiotic and biotic stress responses: a current view from the points of convergence in the stress signaling Networks ' (2006 ) 9 Curr. Opin. Plant Biol. : 436 -442.

    • Search Google Scholar
  • Griffith, M., Yaish, M. W. F. (2004) Antifreeze proteins in overwintering plants: a tale of two activities. Trends Plant Sci. 9, 399–405.

    Yaish M. W. F. , 'Antifreeze proteins in overwintering plants: a tale of two activities ' (2004 ) 9 Trends Plant Sci. : 399 -405.

    • Search Google Scholar
  • Guo, Z., Tan, H., Zhu, Z., Lu, S., Zhou, B. (2005) Effect of intermediates on ascorbic acid and oxalate biosynthesis of rice and in relation to its stress resistance. Plant Physiol. Biochem. 43, 955–962.

    Zhou B. , 'Effect of intermediates on ascorbic acid and oxalate biosynthesis of rice and in relation to its stress resistance ' (2005 ) 43 Plant Physiol. Biochem. : 955 -962.

    • Search Google Scholar
  • Hamilton, E. W., Heckathorn, S. A. (2001) Mitochondrial adaptations to NaCl complex I is protected by antioxidants and small heat shock proteins, whereas complex II is protected by proline and betaine. Plant Physiol. 126, 1266–1274.

    Heckathorn S. A. , 'Mitochondrial adaptations to NaCl complex I is protected by antioxidants and small heat shock proteins, whereas complex II is protected by proline and betaine ' (2001 ) 126 Plant Physiol. : 1266 -1274.

    • Search Google Scholar
  • Heath, R. L., Packer, L. (1968) Photoperoxidation in isolated chloroplast, I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 125, 180–198.

    Packer L. , 'Photoperoxidation in isolated chloroplast, I. Kinetics and stoichiometry of fatty acid peroxidation ' (1968 ) 125 Arch. Biochem. Biophys. : 180 -198.

    • Search Google Scholar
  • Heerden, P. D. R., Krüger, G. H. J. (2002) Separately and simultaneously induced dark chilling and drought stress effects on photosynthesis, proline accumulation and antioxidant metabolism in soybean. J. Plant Physiol. 159, 1077–1086.

    Krüger G. H. J. , 'Separately and simultaneously induced dark chilling and drought stress effects on photosynthesis, proline accumulation and antioxidant metabolism in soybean ' (2002 ) 159 J. Plant Physiol. : 1077 -1086.

    • Search Google Scholar
  • Holá, D., Kočová, M., Rothová, O., Wilhelmová, N., Benešova, M. (2007) Recovery of maize (Zea mays L.) inbreds and hybrids from chilling stress of various duration: Photosynthesis and antioxidant enzymes. J. Plant Physiol. 164, 868–877.

    Benešova M. , 'Recovery of maize (Zea mays L.) inbreds and hybrids from chilling stress of various duration: Photosynthesis and antioxidant enzymes ' (2007 ) 164 J. Plant Physiol. : 868 -877.

    • Search Google Scholar
  • Kader, D. Z. A., Saleh, A. A. H., Elmeleigy, S. A., Dosoky, N. S. (2011) Chilling-induced oxidative stress and polyamines regulatory role in two wheat varieties. Jtusci. 5, 14–24.

    Dosoky N. S. , 'Chilling-induced oxidative stress and polyamines regulatory role in two wheat varieties ' (2011 ) 5 Jtusci. : 14 -24.

    • Search Google Scholar
  • Kaur, G., Kumar, S., Thakur, P., Malik, J. A., Bhandhari, K., Sharma, K. D., Nayyar, H. (2011) Involvement of proline in response of chickpea (Cicer arietinum L.) to chilling stress at reproductive stage. Sci. Hortic. 128, 174–181.

    Nayyar H. , 'Involvement of proline in response of chickpea (Cicer arietinum L.) to chilling stress at reproductive stage ' (2011 ) 128 Sci. Hortic. : 174 -181.

    • Search Google Scholar
  • Labudda, M. (2013) Lipid peroxidation as a biochemical marker for oxidative stress during drought. An effective tool for plant breeding. www.e-wydawnictwo.eu/Document/Documentpreview/3342.

    Labudda M. , '', in Lipid peroxidation as a biochemical marker for oxidative stress during drought , (2013 ) -.

  • Lattanzio, V., Lattanzio, V. M. T., Cardinali, A. (2006) Role of phenolics in the resistance mechanisms of plants against fungal pathogens and insects. In: Imperato, F. (ed.) Phytochemistry: Advances in Research. ISBN: 81-308-0034-9. India, pp. 23–67.

    Cardinali A. , '', in Phytochemistry: Advances in Research , (2006 ) -.

  • Li, Q., Yu, B., Gao, Y., Dai, A. H., Bai, J. G. (2011) Cinnamic acid pretreatment mitigates chilling stress of cucumber leaves through altering antioxidant enzyme activity. J. Plant Physiol. 168, 927–934.

    Bai J. G. , 'Cinnamic acid pretreatment mitigates chilling stress of cucumber leaves through altering antioxidant enzyme activity ' (2011 ) 168 J. Plant Physiol. : 927 -934.

    • Search Google Scholar
  • Lichtenthaler, H. K., Wellburn, A. R. (1983) Determinations of total carotenoids and chlorophylls a and b of leaf exracts in different solvents. Biochem. Soc. Trans. 11, 591–592.

    Wellburn A. R. , 'Determinations of total carotenoids and chlorophylls a and b of leaf exracts in different solvents ' (1983 ) 11 Biochem. Soc. Trans. : 591 -592.

    • Search Google Scholar
  • Mandal, S., Yadav, S., Yadav, S., Nema, R. K. (2009) Antioxidants: A review. J. Chem. Pharm. Res. 1, 102–104.

    Nema R. K. , 'Antioxidants: A review ' (2009 ) 1 J. Chem. Pharm. Res. : 102 -104.

  • Mukherjee, S. P., Choudhuri, M. A. (1983) Implication of water stress induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiol. Plantarum 58, 166–170.

    Choudhuri M. A. , 'Implication of water stress induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings ' (1983 ) 58 Physiol. Plantarum : 166 -170.

    • Search Google Scholar
  • Padda, M. S., Picha, D. H. (2008) Effect of low temperature storage on phenolic composition and antioxidant activity of sweetpotatoes. Postharvest Biol. Tec. 47, 176–180.

    Picha D. H. , 'Effect of low temperature storage on phenolic composition and antioxidant activity of sweetpotatoes ' (2008 ) 47 Postharvest Biol. Tec. : 176 -180.

    • Search Google Scholar
  • Perez-Ilzarbe, J., Hernandez, T., Estrella I., Vendrell M. (1997) Cold storage of apples (cv. Granny Smith) and changes in phenolic compounds. Z. Lebensm. Unters. Forsch. 204, 52–55.

    Vendrell M. , 'Cold storage of apples (cv. Granny Smith) and changes in phenolic compounds ' (1997 ) 204 Z. Lebensm. Unters. Forsch. : 52 -55.

    • Search Google Scholar
  • Prohens, J., Ruiz, J. J., Nuez, F. (2000) Growing cycles for a new crop, the pepino, in the Spanish mediterranean. Acta Hort. 523, 53–58.

    Nuez F. , 'Growing cycles for a new crop, the pepino, in the Spanish mediterranean ' (2000 ) 523 Acta Hort. : 53 -58.

    • Search Google Scholar
  • Ramakrishna, A., Ravishankar, G. A. (2011) Influence of abiotic stress signals on secondary metabolites in plants. Plant Signal Behav. 6, 1720–1731.

    Ravishankar G. A. , 'Influence of abiotic stress signals on secondary metabolites in plants ' (2011 ) 6 Plant Signal Behav. : 1720 -1731.

    • Search Google Scholar
  • Rasheed, R., Wahid, A., Ashraf, M., Basra, S. M. A. (2010) Role of proline and glycinebetaine in improving chilling stresstolerance in sugarcane buds at sprouting. International J. Agric. Biol. 12, 1–8.

    Basra S. M. A. , 'Role of proline and glycinebetaine in improving chilling stresstolerance in sugarcane buds at sprouting ' (2010 ) 12 International J. Agric. Biol. : 1 -8.

    • Search Google Scholar
  • Ruiz, J. J., Nuez, F. (1997) The pepino (Solanum muricatum Ait): an alternative crop for areas affected by moderate salinity. Hort. Sci. 32, 649–652.

    Nuez F. , 'The pepino (Solanum muricatum Ait): an alternative crop for areas affected by moderate salinity ' (1997 ) 32 Hort. Sci. : 649 -652.

    • Search Google Scholar
  • Sevengor, S., Yasar, F., Kusvuran, S., Ellialtioglu, S. (2011) The effect of salt stress on growth, chlorophyll content, lipid peroxidation and antioxidative enzymes of pumpkin seedling. Afr. J. Agric. Res. 6, 4920–4924.

    Ellialtioglu S. , 'The effect of salt stress on growth, chlorophyll content, lipid peroxidation and antioxidative enzymes of pumpkin seedling ' (2011 ) 6 Afr. J. Agric. Res. : 4920 -4924.

    • Search Google Scholar
  • Slinkard, K., Singleton, V. L. (1977) Total phenol analyses: automation and comparison with manual methods. Am. J. Enol. Vitic. 28, 49–55.

    Singleton V. L. , 'Total phenol analyses: automation and comparison with manual methods ' (1977 ) 28 Am. J. Enol. Vitic. : 49 -55.

    • Search Google Scholar
  • Smirnoff, N. (2000) Ascorbic acid: metabolism and functions of a multi-facetted molecule. Curr. Opin. Plant Biol. 3, 229–235.

    Smirnoff N. , 'Ascorbic acid: metabolism and functions of a multi-facetted molecule ' (2000 ) 3 Curr. Opin. Plant Biol. : 229 -235.

    • Search Google Scholar
  • Tambussi, E. A., Bartoli, C. G., Guiamet, J. J., Beltrano, J., Araus, J. L. (2004) Oxidative stress and photodamage at low temperatures in soybean (Glycine max L. Merr.) leaves. Plant Sci. 167, 19–26.

    Araus J. L. , 'Oxidative stress and photodamage at low temperatures in soybean (Glycine max L ' (2004 ) 167 Merr.) leaves. Plant Sci. : 19 -26.

    • Search Google Scholar
  • Thakur, P., Nayyar, H. (2013) Facing the cold stress by plants in the changing environment: sensing, signaling, and defending mechanisms. In: Tuteja, N., Gill, S. S. (eds) Plant Acclimation to Environmental Stress. Chapter 2. DOI 10.1007/978-1-4614-5001-6_2 , © Springer Science+Business Media, New York, pp. 29–69

    Nayyar H. , '', in Plant Acclimation to Environmental Stress , (2013 ) -.

  • Xu, S., Li, Y., Hu, J., Guan, Y., Ma, W., Zheng, Y., Zhu, S. (2010) Responses of antioxidant enzymes to chilling stress in tobacco seedlings. Agr. Sci. China. 9, 1594–1601.

    Zhu S. , 'Responses of antioxidant enzymes to chilling stress in tobacco seedlings ' (2010 ) 9 Agr. Sci. China. : 1594 -1601.

    • Search Google Scholar
  • Yalçin, H. (2010) Effect of ripening period on composition of pepino (Solanum muricatum) fruit grown in Turkey. Afr. J. Biotechnol. 9, 3901–3903.

    Yalçin H. , 'Effect of ripening period on composition of pepino (Solanum muricatum) fruit grown in Turkey ' (2010 ) 9 Afr. J. Biotechnol. : 3901 -3903.

    • Search Google Scholar
  • Zhang, W., Jiang, B., Li, W., Song, H., Yu, Y., Chen, J. (2009) Polyamines enhance chilling tolerance of cucumber (Cucumis sativus L.) through modulating antioxidative system. Sci. Hortic. 122, 200–208.

    Chen J. , 'Polyamines enhance chilling tolerance of cucumber (Cucumis sativus L.) through modulating antioxidative system ' (2009 ) 122 Sci. Hortic. : 200 -208.

    • Search Google Scholar
  • Zhu, J. J., Li, Y. R., Liao, J. X. (2013) Involvement of anthocyanins in the resistance to chillinginduced oxidative stress in Saccharum officinarum L. leaves. Plant Physiol. Bioch. 73, 427–433.

    Liao J. X. , 'Involvement of anthocyanins in the resistance to chillinginduced oxidative stress in Saccharum officinarum L. leaves ' (2013 ) 73 Plant Physiol. Bioch. : 427 -433.

    • Search Google Scholar