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  • 1 Sinop University Faculty of Arts and Sciences, Biology Sinop Turkey
  • 2 The Black Sea Agriculture Resources Institute Samsun Turkey
  • 3 19 Mayis University Faculty of Arts and Sciences, Biology Samsun Turkey
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In the present study, the effect of ascorbic acid (5 mM) on some physiological parameters and three hormones (auxin, abscisic acid, salicylic acid) was determined under heat stress (40 °C) in maize tolerant cv. (MAY 69) and sensitive cv. SHEMAL (SH) at 0 h, 4 h and 8 h. Heat stress reduced total chlorophyll content (CHL), relative water content (RWC) and stomatal conductance (g s) in SH but did not lead to changes in MAY 69 at 4 h and 8 h. However, pretreatment with ascorbic acid increased (CHL), (RWC) and (g s) in SH under heat stress while it reduced MDA content significantly in both cv. We also observed that heat stress led to a reduction in SA level but increased ABA and IAA levels in SH, whereas it increased SA and IAA levels but did not change ABA level in MAY 69 at 4 h. Furthermore, in SH, ASC application under heat stress increased SA level and decreased IAA and ABA levels at 4 h, but it had no effect on SA and ABA at 8 h.

  • Arrigoni, O., De Tullio, M. C. (2002) Ascorbic acid: much more than an antioxidant. Biochim. Biophys. Acta 1569, 1–9.

    Tullio M. C. , 'Ascorbic acid: much more than an antioxidant ' (2002 ) 1569 Biochim. Biophys. Acta : 1 -9.

    • Search Google Scholar
  • Athar, H. R., Khan, A., Ashraf, M. (2008) Exogenously applied ascorbic acid alleviates salt-induced oxidative stress in wheat. Environ. Exp. Bot. 63, 224–231.

    Ashraf M. , 'Exogenously applied ascorbic acid alleviates salt-induced oxidative stress in wheat ' (2008 ) 63 Environ. Exp. Bot. : 224 -231.

    • Search Google Scholar
  • Azzedine, F., Gherroucha, H., Baka, M. (2011) Improvement of salt tolerance in durum wheat by ascorbic acid application. J. Stress Physiol. Biochem. 7, 27–37.

    Baka M. , 'Improvement of salt tolerance in durum wheat by ascorbic acid application ' (2011 ) 7 J. Stress Physiol. Biochem. : 27 -37.

    • Search Google Scholar
  • Baninasab, B. (2010) Induction of drought tolerance by salicylic acid in seedlings of cucumber (Cucumis sativus L.). J. Hortic. Sci. Biotechnol. 85, 191–196.

    Baninasab B. , 'Induction of drought tolerance by salicylic acid in seedlings of cucumber (Cucumis sativus L.) ' (2010 ) 85 J. Hortic. Sci. Biotechnol. : 191 -196.

    • Search Google Scholar
  • Barth, C., Moeder, W., Klessig, D. F., Conklin, P. L. (2004) The timing of senesence and response to pathogens is altered in the ascorbate-deficient Arabidopsis mutant vitamin C-1. Plant Physiol. 134, 1784–1792.

    Conklin P. L. , 'The timing of senesence and response to pathogens is altered in the ascorbate-deficient Arabidopsis mutant vitamin C-1 ' (2004 ) 134 Plant Physiol. : 1784 -1792.

    • Search Google Scholar
  • Chen, Z., Gallie, D. R. (2004) The ascorbic acid redox state controls guard cell signaling and stomatal movement. Plant Cell 16, 1143–1162.

    Gallie D. R. , 'The ascorbic acid redox state controls guard cell signaling and stomatal movement ' (2004 ) 16 Plant Cell : 1143 -1162.

    • Search Google Scholar
  • Clarke, S. M., Mur, L. A. J., Wood, J. E., Scott, I. M. (2004) Salicylic acid dependent signaling promotes basal thermotolerance but is not essential for acquired thermotolerance in Arabidopsis thaliana. Plant J. 38, 432–447.

    Scott I. M. , 'Salicylic acid dependent signaling promotes basal thermotolerance but is not essential for acquired thermotolerance in Arabidopsis thaliana ' (2004 ) 38 Plant J. : 432 -447.

    • Search Google Scholar
  • Conklin, P. L., Barth, C. (2004) Ascorbic acid, a familiar small molecule interwined in the response of plants to ozone, pathogens, and the onset of senecence. Plant Cell Environ. 27, 959–970.

    Barth C. , 'Ascorbic acid, a familiar small molecule interwined in the response of plants to ozone, pathogens, and the onset of senecence ' (2004 ) 27 Plant Cell Environ. : 959 -970.

    • Search Google Scholar
  • Davey, M. W., Montagu, M. V., Inze, D., Sanmartin, M., Kanellis, A., Smirnoff, N., Benzie, I. J. J., Strain, J. J., Favell, D., Fletcher, J. (2000) Plant L-ascorbic acid: chemistry, function, metabolism, bioavailability and effects of processing. J. Sci. Food Agric. 80, 825–860.

    Fletcher J. , 'Plant L-ascorbic acid: chemistry, function, metabolism, bioavailability and effects of processing ' (2000 ) 80 J. Sci. Food Agric. : 825 -860.

    • Search Google Scholar
  • Dolatabadian, A., Sanavi, A. M., Sharifi, M. (2009) Alleviation of water deficit stress effects by foliar application of ascorbic acid on Zea mays L. J. Agron. Crop Sci. 195, 347–355.

    Sharifi M. , 'Alleviation of water deficit stress effects by foliar application of ascorbic acid on Zea mays L ' (2009 ) 195 J. Agron. Crop Sci. : 347 -355.

    • Search Google Scholar
  • Du, H., Liu, H., Lizhong, X. (2013) Endogenous auxin and jasmonicacid level are differentially modulated by a biotic stresses in rice. Frontiers Plant Sci. 4, 1–10.

    Lizhong X. , 'Endogenous auxin and jasmonicacid level are differentially modulated by a biotic stresses in rice ' (2013 ) 4 Frontiers Plant Sci. : 1 -10.

    • Search Google Scholar
  • Flores, M. I. A., Romero-Gonzalez, R., Garrido Frenich, A., Luis Martinez Vidal, J. (2011) QuEChERS-based extraction procedure for multifamily analysis of phytohormones in vegetables by UHPLC-MS/MS. J. Sep. Sci. 34, 1517–1524.

    Luis Martinez Vidal J. , 'QuEChERS-based extraction procedure for multifamily analysis of phytohormones in vegetables by UHPLC-MS/MS ' (2011 ) 34 J. Sep. Sci. : 1517 -1524.

    • Search Google Scholar
  • Fujita, M., Fujita, Y., Noutoshi, Y., Takahashi, F., Naruska, Y., Yamaguchi-Shinozaki, K., Shinozaki, K. (2006) Crosstalk between abiotic and biotic stress response: a current view from the points of convergence in the stress signalling networks. Curr. Opin. Plan Biol. 9, 436–442.

    Shinozaki K. , 'Crosstalk between abiotic and biotic stress response: a current view from the points of convergence in the stress signalling networks ' (2006 ) 9 Curr. Opin. Plan Biol. : 436 -442.

    • Search Google Scholar
  • Gong, M., Li, Y.-J., Chen, S. Z. (1998) Abscisic acid induced thermotolerance in maize seedlings is mediated by Ca2 and associated with antioxidant systems. J. Plant Physiol. 153, 488–496.

    Chen S. Z. , 'Abscisic acid induced thermotolerance in maize seedlings is mediated by Ca2 and associated with antioxidant systems ' (1998 ) 153 J. Plant Physiol. : 488 -496.

    • Search Google Scholar
  • Hassanein, R. A., Bassony, F. M., Barakat, D. M., Khalil, R. R. (2009) Physiological effects of nicotinamide and ascorbic acid on Zea mays plant grown under salinity stress. 1. Changes in growth, some relevant metabolic activities and oxidative defense systems. Res. J. Agric. Biol. Sci. 5, 72–81.

    Khalil R. R. , 'Physiological effects of nicotinamide and ascorbic acid on Zea mays plant grown under salinity stress. 1. Changes in growth, some relevant metabolic activities and oxidative defense systems ' (2009 ) 5 Res. J. Agric. Biol. Sci. : 72 -81.

    • Search Google Scholar
  • Hu, J. F., Li, G. F., Gao, Z. H., Chen, L., Ren, H. B., Jia, W. S. (2005) Regulation of water deficitinduced abscisic acid accumulation by apoplastic ascorbic acid in maize seedlings. J. Integr. Plant Biol. 47, 1335–1344.

    Jia W. S. , 'Regulation of water deficitinduced abscisic acid accumulation by apoplastic ascorbic acid in maize seedlings ' (2005 ) 47 J. Integr. Plant Biol. : 1335 -1344.

    • Search Google Scholar
  • Hurkman, W. J., Vensel, W. H., Tanaka, C. K., Whitehand, L., Altenbach, S. B. (2009) Effect of high temperature on albumin and globulin accumulation in the endosperm proteome of the developing wheat grain. J. Cereal Sci. 49, 12–23.

    Altenbach S. B. , 'Effect of high temperature on albumin and globulin accumulation in the endosperm proteome of the developing wheat grain ' (2009 ) 49 J. Cereal Sci. : 12 -23.

    • Search Google Scholar
  • Kang, H., Saltveit, M. E. (2002) Chilling tolerance of maize cucumber and rice seedling leaves and roots are differentially affected by salyclic acid. Physiol. Plant. 115, 571–576.

    Saltveit M. E. , 'Chilling tolerance of maize cucumber and rice seedling leaves and roots are differentially affected by salyclic acid ' (2002 ) 115 Physiol. Plant. : 571 -576.

    • Search Google Scholar
  • Khan, T. A., Mazid, M., Mohammad, F. (2011) A review of ascorbic acid potentialities against oxidative stress induced in plants. J. Agrobiol. 28, 97–111.

    Mohammad F. , 'A review of ascorbic acid potentialities against oxidative stress induced in plants ' (2011 ) 28 J. Agrobiol. : 97 -111.

    • Search Google Scholar
  • Kumar, S., Kaur, R., Kaur, N., Bhandhari, K., Kaushal, N., Gupta, K., Bains, T. S., Nayyar, H. (2011) Heat-stress induced inhibition in growth and chlorosis in mungbean (Phaseolus aureus Roxb.) is partly mitigated by ascorbic acid application and is related to reduction in oxidative stress. Acta Physiol. Plant 33, 6, 2091–2101.

    Nayyar H. , 'Heat-stress induced inhibition in growth and chlorosis in mungbean (Phaseolus aureus Roxb.) is partly mitigated by ascorbic acid application and is related to reduction in oxidative stress ' (2011 ) 33 Acta Physiol. Plant : 2091 -2101.

    • Search Google Scholar
  • Kus, J. V., Zaton, K., Sarkar, R., Cameron, R. K. (2002) Age-related resistance in arabidopsis is a developmentally regulated defense responseto Pseudomonas syringae. Plant Cell 14, 479–490.

    Cameron R. K. , 'Age-related resistance in arabidopsis is a developmentally regulated defense responseto Pseudomonas syringae ' (2002 ) 14 Plant Cell : 479 -490.

    • Search Google Scholar
  • Lichtenthaler, H. K., Wellburn, A. R. (1983) Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem. Soc. Trans. 11, 591–592.

    Wellburn A. R. , 'Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents ' (1983 ) 11 Biochem. Soc. Trans. : 591 -592.

    • Search Google Scholar
  • Madhava, R. K.V., Sresty, T. V. S. (2000) Antioxidative parameters in the seedlings of pigeon pea (Cajanus cajan L. millspaugh) in response to Zn and Ni stresses. Plant Sci. 157, 113–128.

    Sresty T. V. S. , 'Antioxidative parameters in the seedlings of pigeon pea (Cajanus cajan L. millspaugh) in response to Zn and Ni stresses ' (2000 ) 157 Plant Sci. : 113 -128.

    • Search Google Scholar
  • Miguel, A., Rosales, J., Ruiz, M., Hernandez, J., Soriano, T., Castilla, N., Romero, L. (2006) Antioxidant content and ascorbate metabolism in cherry tomato exocarp in relation to temperature and solar radiation. J. Sci. Food Agric. 86, 1525–1551.

    Romero L. , 'Antioxidant content and ascorbate metabolism in cherry tomato exocarp in relation to temperature and solar radiation ' (2006 ) 86 J. Sci. Food Agric. : 1525 -1551.

    • Search Google Scholar
  • Mukherjee, M., Larrimore, K. E., Ahmed, N. J., Bedick, T. S., Barghouthi, N. T., Tra, M. B., Barth, C. (2010) Ascorbic acid deficiency in Arabidopsis induces constitutive priming that is dependent on hydrogen peroxide, salicylic acid, and the NPR1 gene. Mol. Plant Microbe Interact. 23, 340–351.

    Barth C. , 'Ascorbic acid deficiency in Arabidopsis induces constitutive priming that is dependent on hydrogen peroxide, salicylic acid, and the NPR1 gene ' (2010 ) 23 Mol. Plant Microbe Interact. : 340 -351.

    • Search Google Scholar
  • Noctor, G., Foyer, C. H. (1998) Ascorbate and glutathione: keeping active oxygen under control. Annu Rev. Plant Physiol. Plant Mol. Biol. 49, 249–279.

    Foyer C. H. , 'Ascorbate and glutathione: keeping active oxygen under control ' (1998 ) 49 Annu Rev. Plant Physiol. Plant Mol. Biol. : 249 -279.

    • Search Google Scholar
  • Okamura, M. (1980) An improved method for determination of L-ascorbic acid and L-dehydro ascorbic acid in blood plasma. Clinica Chimica Acta 103, 259–268.

    Okamura M. , 'An improved method for determination of L-ascorbic acid and L-dehydro ascorbic acid in blood plasma ' (1980 ) 103 Clinica Chimica Acta : 259 -268.

    • Search Google Scholar
  • Park, J. E., Park, J. Y., Kim, Y. S., Staswick, P. E., Jeon, J., Yun, J., Kim, S. Y., Kim, J., Lee, Y. H., Park, C.-M. (2007) GH3-mediatedauxin homeostasis links growth regulation with stress adaptation response in Arabidopsis. J. Biol. Chem. 282, 10036–10046.

    Park C.-M. , 'GH3-mediatedauxin homeostasis links growth regulation with stress adaptation response in Arabidopsis ' (2007 ) 282 J. Biol. Chem. : 10036 -10046.

    • Search Google Scholar
  • Parry, M. A. J., Andralojc, P. J., Khan, S., Lea, P. J., Keys, A. J. (2002) Rubisco activity: effects of drought stress. Ann. Bot. 89, 833–839.

    Keys A. J. , 'Rubisco activity: effects of drought stress ' (2002 ) 89 Ann. Bot. : 833 -839.

  • Pastori, G. M., Kiddle, G., Antoniw, J., Bernard, S., Veljovic-Jovanovic, S., Verrier, P. J., Noctor, G., Foyer, C. H. (2003) Leaf vitamin C contents modulate plant defense transcripts and regulates genes that control development through hormone signalling. Plant Cell. 15, 939–951.

    Foyer C. H. , 'Leaf vitamin C contents modulate plant defense transcripts and regulates genes that control development through hormone signalling ' (2003 ) 15 Plant Cell. : 939 -951.

    • Search Google Scholar
  • Pavet, V., Olmos, E., Kiddle, G., Mowla, S., Kumar, S., Antoniw, J., Alvarez, M. E., Foyer, C. H. (2005) Ascorbic acid deficiency activates cell death and disease resistance responses in Arabidopsis. Plant Physiol. 139, 1291–1303.

    Foyer C. H. , 'Ascorbic acid deficiency activates cell death and disease resistance responses in Arabidopsis ' (2005 ) 139 Plant Physiol. : 1291 -1303.

    • Search Google Scholar
  • Reddy, A. R., Visvanatha Chaitanya, K., Vivekanandan, M. (2004) Drought induces responses of photosynthesis and antioxidant metabolism in higher plants. J. Plant Physiol. 161, 1189–1202.

    Vivekanandan M. , 'Drought induces responses of photosynthesis and antioxidant metabolism in higher plants ' (2004 ) 161 J. Plant Physiol. : 1189 -1202.

    • Search Google Scholar
  • Ristic, Z., Bukovnik, U., Prasad, P. V. V. (2007) Correlation between heat stability of thylakoid membranes and loss of chlorophyll in winter wheat 1140 under heat stress. Crop Sci. 47, 2067–2073.

    Prasad P. V. V. , 'Correlation between heat stability of thylakoid membranes and loss of chlorophyll in winter wheat 1140 under heat stress ' (2007 ) 47 Crop Sci. : 2067 -2073.

    • Search Google Scholar
  • Sairam, R. K., Roa, K. V., Srivastava, G. C. (2002) Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Sci. 163, 1037–1046.

    Srivastava G. C. , 'Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration ' (2002 ) 163 Plant Sci. : 1037 -1046.

    • Search Google Scholar
  • Savicka, M., Škute, N. (2010) Effects of high temperature on malondialdehyde content, superoxide production and growth changes in wheat seedlings (Triticum aestivum L.). Ekologija 56, 26–33.

    Škute N. , 'Effects of high temperature on malondialdehyde content, superoxide production and growth changes in wheat seedlings (Triticum aestivum L.) ' (2010 ) 56 Ekologija : 26 -33.

    • Search Google Scholar
  • Shalata, A., Neumann, P. M. (2001) Exogenous ascorbic acid (vitamin C) increases resistance to salt tolerance and reduced lipid peroxidation, J. Exp. Bot. 364, 2207–2211.

    Neumann P. M. , 'Exogenous ascorbic acid (vitamin C) increases resistance to salt tolerance and reduced lipid peroxidation ' (2001 ) 364 J. Exp. Bot. : 2207 -2211.

    • Search Google Scholar
  • Smart, R. E., Bingham, G. E. (1974) Rapid estimates of relative water content. Plant Physiol. 53, 258–260.

    Bingham G. E. , 'Rapid estimates of relative water content ' (1974 ) 53 Plant Physiol. : 258 -260.

    • Search Google Scholar
  • Smirnoff, N. (1996) The function and metabolism of ascorbic acid recent in plants. Ann Bot. 78, 661–669.

    Smirnoff N. , 'The function and metabolism of ascorbic acid recent in plants ' (1996 ) 78 Ann Bot. : 661 -669.

    • Search Google Scholar
  • Thompson, J. E., Legge, R. L., Barber, R. F. (1987) The role of free radicals in senescence and wounding. New Phytol. 105, 317–344.

    Barber R. F. , 'The role of free radicals in senescence and wounding ' (1987 ) 105 New Phytol. : 317 -344.

    • Search Google Scholar
  • Valliyodan, B., Nguyen, H. T. (2006) Understanding regulatory networks and engineering for enhanced drought tolerance in plants. Curr. Opin. Plant Biol. 9, 189–195.

    Nguyen H. T. , 'Understanding regulatory networks and engineering for enhanced drought tolerance in plants ' (2006 ) 9 Curr. Opin. Plant Biol. : 189 -195.

    • Search Google Scholar
  • Veljovic-Jovanovic, S. D., Pignocchi, C., Noctor, G., Foyer, C. H. (2001) Low Ascorbic acid in the vtc1 mutant of Arabidopsis is associated with decreased growth and intracellular redistribution of the antioxidant system. Plant Physiol. 127, 426–435.

    Foyer C. H. , 'Low Ascorbic acid in the vtc1 mutant of Arabidopsis is associated with decreased growth and intracellular redistribution of the antioxidant system ' (2001 ) 127 Plant Physiol. : 426 -435.

    • Search Google Scholar
  • Wen, X. P., Pang, X. M., Matsuda, N., Kita, M., Inoue, H., Hao, Y. J. (2008) Overexpression of the apple spermidine synthase gene in pear confers multiple abiotic stress tolerance by altering polyamine titers. Transgenic Res. 17, 251–263.

    Hao Y. J. , 'Overexpression of the apple spermidine synthase gene in pear confers multiple abiotic stress tolerance by altering polyamine titers ' (2008 ) 17 Transgenic Res. : 251 -263.

    • Search Google Scholar
  • Wilkinson, S. Davies, W. J. (2002) ABA-based chemical signaling: The coordination of responses to stress in plants. Plant Cell Environ. 25, 195–210.

    Davies W. J. , 'ABA-based chemical signaling: The coordination of responses to stress in plants ' (2002 ) 25 Plant Cell Environ. : 195 -210.

    • Search Google Scholar
  • Zhang, J., Davies, W. J. (1990) Changes in the concentration of ABA in xylem sap as a function of changing soil water status can account for changes in leaf conductance and growth. Plant Cell Environ. 13, 277–285.

    Davies W. J. , 'Changes in the concentration of ABA in xylem sap as a function of changing soil water status can account for changes in leaf conductance and growth ' (1990 ) 13 Plant Cell Environ. : 277 -285.

    • Search Google Scholar