Acta Biologica Hungarica
Volume/Issue:
Volume 66: Issue 1
Stability of CART peptide expression in the nucleus accumbens in aging
Authors:
Simon Armbruszt University of Pécs Institute of Nutritional Sciences and Dietetics, Faculty of Health Sciences Pécs Hungary

Search for other papers by Simon Armbruszt in
Current site
Google Scholar
PubMed Close
,
Mária Figler University of Pécs Institute of Nutritional Sciences and Dietetics, Faculty of Health Sciences Pécs Hungary

Search for other papers by Mária Figler in
Current site
Google Scholar
PubMed Close
, and
Hajnalka Ábrahám University of Pécs Central Electron Microscopic Laboratory, Faculty of Medicine Pécs Hungary

Search for other papers by Hajnalka Ábrahám in
Current site
Google Scholar
PubMed Close
Pages:
1–13
Online Publication Date:
04 Mar 2015
Publication Date:
01 Mar 2015
Article Category:
Research Article
DOI:
https://doi.org/10.1556/abiol.66.2015.1.1
Keywords:
Food intake; anorexigenic peptide; reward system; age-related; densitometry
Restricted access

Aging is accompanied by changes of several anorexigenic and orexigenic neuropeptides expressed in various brain areas that control food intake and these changes correlate with senescent anorexia. During aging expression of cocaine- and amphetamine-regulated transcript (CART) peptide was reported to be reduced in the hypothalamic nuclei related to food intake. Although CART peptide is abundant in the nucleus accumbens that also plays a crucial role in the food intake regulation, no data is available about the CART peptide expression in this region through aging. In the present study, CART peptide immunoreactivity was compared in the nucleus accumbens of young adult (4- and 7-month-old) middle-aged (15-month-old) and aging (25–32-month-old) Long-Evans rats. The density of CART-immunoreactive cells and axon terminals in the nucleus accumbens was measured with computer-aided densitometry. CART-immunodensity was similar in the old rats and in the younger animals without significant difference between age groups. In addition, no gender-difference was observed when CART-immunoreactivities in the nucleus accumbens of male and female animals were compared. Our results indicate that CART peptide expression in the nucleus accumbens is stable in adults and does not change with age.

  • Abraham, H., Covasa, M., Hajnal, A. (2009) Cocaine- and amphetamine-regulated transcript peptide immunoreactivity in the brain of the CCK-1 receptor deficient obese OLETF rat. Exp. Brain Res. 196, 545–556.

    Hajnal A. , 'Cocaine- and amphetamine-regulated transcript peptide immunoreactivity in the brain of the CCK-1 receptor deficient obese OLETF rat ' (2009 ) 196 Exp. Brain Res. : 545 -556 .

    • Search Google Scholar

  • Akimoto-Takano, S., Sakurai, C., Kanai, S., Hosoya, H., Ohta, M., Miyasaka, K. (2005) Differences in the appetite-stimulating effect of orexin, neuropeptide Y and ghrelin among young, adult and old rats. Neuroendocrinology 82, 256–263.

    Miyasaka K. , 'Differences in the appetite-stimulating effect of orexin, neuropeptide Y and ghrelin among young, adult and old rats ' (2005 ) 82 Neuroendocrinology : 256 -263 .

    • Search Google Scholar

  • Armbruszt, S., Abraham, H., Figler, M., Kozicz, T., Hajnal, A. (2013) Cocaine- and amphetamine-regulated transcript (CART) peptide immunoreactivity in feeding- and reward-related areas of young OLETF rats. J. Chem. Neuroanat. 50–51, 75–84.

    Hajnal A. , 'Cocaine- and amphetamine-regulated transcript (CART) peptide immunoreactivity in feeding- and reward-related areas of young OLETF rats ' (2013 ) 50–51 J. Chem. Neuroanat. : 75 -84 .

    • Search Google Scholar

  • Balaskó, M., Soós, S., Párniczky, A., Koncsecskó-Gáspár, M., Székely, M., Pétervári, E. (2012) Anorexic effect of peripheral cholecystokinin (CCK) varies with age and body composition (short communication). Acta Physiol. Hung. 99, 166–172.

    Pétervári E. , 'Anorexic effect of peripheral cholecystokinin (CCK) varies with age and body composition (short communication) ' (2012 ) 99 Acta Physiol. Hung. : 166 -172 .

    • Search Google Scholar

  • Balasko, M., Rostas, I., Furedi, N., Miko, A., Tenk, J., Cseplo, P., Koncsecsko-Gaspar, M., Soos, S., Szekely, M., Petervari, E. (2013) Age and nutritional state influence the effects of cholecystokinin on energy balance. Exp. Gerontol. 48, 1180–1188.

    Petervari E. , 'Age and nutritional state influence the effects of cholecystokinin on energy balance ' (2013 ) 48 Exp. Gerontol. : 1180 -1188 .

    • Search Google Scholar

  • Black, B. J. Jr., McMahan, C. A., Masoro, E. J., Ikeno, Y., Katz, M. S. (2003) Senescent terminal weight loss in the male F344 rat. Am. J. Physiol. Regul. Integr. Comp. Physiol. 284, R336–342.

    Katz M. S. , 'Senescent terminal weight loss in the male F344 rat ' (2003 ) 284 Am. J. Physiol. Regul. Integr. Comp. Physiol. : R336 -342 .

    • Search Google Scholar

  • Blanton, C. A., Horwitz, B. A., Murtagh-Mark, C., Gietzen, D. W., Griffey, S. M., McDonald, R. B. (1998) Meal patterns associated with the age-related decline in food intake in the Fischer 344 rat. Am. J. Physiol. 275, R1494–1502.

    McDonald R. B. , 'Meal patterns associated with the age-related decline in food intake in the Fischer 344 rat ' (1998 ) 275 Am. J. Physiol. : R1494 -1502 .

    • Search Google Scholar

  • Blanton, C. A., Horwitz, B. A., Blevins, J. E., Hamilton, J. S., Hernandez, E. J., McDonald, R. B. (2001) Reduced feeding response to neuropeptide Y in senescent Fischer 344 rats. Am. J. Physiol. Regul. Integr. Comp. Physiol. 280, R1052–1060.

    McDonald R. B. , 'Reduced feeding response to neuropeptide Y in senescent Fischer 344 rats ' (2001 ) 280 Am. J. Physiol. Regul. Integr. Comp. Physiol. : R1052 -1060 .

    • Search Google Scholar

  • Diao, L. H., Bickford, P. C., Stevens, J. O., Cline, E. J., Gerhardt, G. A. (1997) Caloric restriction enhances evoked DA overflow in striatum and nucleus accumbens of aged Fischer 344 rats. Brain Res. 763, 276–280.

    Gerhardt G. A. , 'Caloric restriction enhances evoked DA overflow in striatum and nucleus accumbens of aged Fischer 344 rats ' (1997 ) 763 Brain Res. : 276 -280 .

    • Search Google Scholar

  • Hubert, G. W., Jones, D. C., Moffett, M. C., Rogge, G., Kuhar, M. J. (2008) CART peptides as modulators of dopamine and psychostimulants and interactions with the mesolimbic dopaminergic system. Biochem. Pharmacol. 75, 57–62.

    Kuhar M. J. , 'CART peptides as modulators of dopamine and psychostimulants and interactions with the mesolimbic dopaminergic system ' (2008 ) 75 Biochem. Pharmacol. : 57 -62 .

    • Search Google Scholar

  • Hunter, R. G., Jones, D., Vicentic, A., Hue, G., Rye, D., Kuhar, M. J. (2006) Regulation of CART mRNA in the rat nucleus accumbens via D3 dopamine receptors. Neuropharmacology 50, 858–864.

    Kuhar M. J. , 'Regulation of CART mRNA in the rat nucleus accumbens via D3 dopamine receptors ' (2006 ) 50 Neuropharmacology : 858 -864 .

    • Search Google Scholar

  • Jernigan, T. L., Archibald, S. L., Fennema-Notestine, C., Gamst, A. C., Stout, J. C., Bonner, J., Hesselink, J. R. (2001) Effects of age on tissues and regions of the cerebrum and cerebellum. Neurobiol. Aging 22, 581–594.

    Hesselink J. R. , 'Effects of age on tissues and regions of the cerebrum and cerebellum ' (2001 ) 22 Neurobiol. Aging : 581 -594 .

    • Search Google Scholar

  • Kaneda, T., Makino, S., Nishiyama, M., Asaba, K., Hashimoto, K. (2001) Differential neuropeptide responses to starvation with ageing. J. Neuroendocrinol. 13, 1066–1075.

    Hashimoto K. , 'Differential neuropeptide responses to starvation with ageing ' (2001 ) 13 J. Neuroendocrinol. : 1066 -1075 .

    • Search Google Scholar

  • Kappeler, L., Gourdji, D., Zizzari, P., Bluet-Pajot, M. T., Epelbaum, J. (2003) Age-associated changes in hypothalamic and pituitary neuroendocrine gene expression in the rat. J. Neuroendocrinol. 15, 592–601.

    Epelbaum J. , 'Age-associated changes in hypothalamic and pituitary neuroendocrine gene expression in the rat ' (2003 ) 15 J. Neuroendocrinol. : 592 -601 .

    • Search Google Scholar

  • Kessler, B. A., Stanley, E. M., Frederick-Duus, D., Fadel, J. (2011) Age-related loss of orexin/hypocretin neurons. Neuroscience 178, 82–88.

    Fadel J. , 'Age-related loss of orexin/hypocretin neurons ' (2011 ) 178 Neuroscience : 82 -88 .

    • Search Google Scholar

  • Koylu, E. O., Couceyro, P. R., Lambert, P. D., Ling, N. C., DeSouza, E. B., Kuhar, M. J. (1997) Immunohistochemical localization of novel CART peptides in rat hypothalamus, pituitary and adrenal gland. J. Neuroendocrinol. 9, 823–833.

    Kuhar M. J. , 'Immunohistochemical localization of novel CART peptides in rat hypothalamus, pituitary and adrenal gland ' (1997 ) 9 J. Neuroendocrinol. : 823 -833 .

    • Search Google Scholar

  • Koylu, E. O., Couceyro, P. R., Lambert, P. D., Kuhar, M. J. (1998) Cocaine- and amphetamine-regulated transcript peptide immunohistochemical localization in the rat brain. J. Comp. Neurol. 391, 115–132.

    Kuhar M. J. , 'Cocaine- and amphetamine-regulated transcript peptide immunohistochemical localization in the rat brain ' (1998 ) 391 J. Comp. Neurol. : 115 -132 .

    • Search Google Scholar

  • Lambert, P. D., Couceyro, P. R., McGirr, K. M., Dall Vechia, S. E., Smith, Y., Kuhar, M. J. (1998) CART peptides in the central control of feeding and interactions with neuropeptide Y. Synapse 29, 293–298.

    Kuhar M. J. , 'CART peptides in the central control of feeding and interactions with neuropeptide Y ' (1998 ) 29 Synapse : 293 -298 .

    • Search Google Scholar

  • Long, X., Liao, W., Jiang, C., Liang, D., Qiu, B., Zhang, L. (2012) Healthy aging: an automatic analysis of global and regional morphological alterations of human brain. Acad. Radiol. 19785–19793.

    Zhang L. , '', in Acad. Radiol. , (2012 ) -.

  • McDonald, R. B., Florez-Duquet, M., Murtagh-Mark, C., Horwitz, B. A. (1996) Relationship between cold-induced thermoregulation and spontaneous rapid body weight loss of aging F344 rats. Am. J. Physiol. Regul. Integr. Comp. Physiol. 271, R1115–R1122.

    Horwitz B. A. , 'Relationship between cold-induced thermoregulation and spontaneous rapid body weight loss of aging F344 rats ' (1996 ) 271 Am. J. Physiol. Regul. Integr. Comp. Physiol. : R1115 -R1122 .

    • Search Google Scholar

  • Orsi, G., Perlaki, G., Kovacs, N., Aradi, M., Papp, Z., Karadi, K., Szalay, C., Karadi, Z., Lenard, L., Tenyi, T., Plozer, E., Gabriel, R., Nagy, F., Doczi, T., Komoly, S., Jokeit, H., Schwarcz, A., Janszky, J. (2011) Body weight and the reward system: the volume of the right amygdala may be associated with body mass index in young overweight men. Brain Imaging Behav. 5, 149–157.

    Janszky J. , 'Body weight and the reward system: the volume of the right amygdala may be associated with body mass index in young overweight men ' (2011 ) 5 Brain Imaging Behav. : 149 -157 .

    • Search Google Scholar

  • Paxinos, G., Watson, C. (1982) The Rat Brain in Stereotaxic Coordinates. Academic Press Australia, Sydney.

    Watson C. , '', in The Rat Brain in Stereotaxic Coordinates , (1982 ) -.

  • Pereira, P. A., Santos, D., Neves, J., Madeira, M. D., Paula-Barbosa, M. M. (2013) Nerve growth factor retrieves neuropeptide Y and cholinergic immunoreactivity in the nucleus accumbens of old rats. Neurobiology of Aging 34, 1988–1995.

    Paula-Barbosa M. M. , 'Nerve growth factor retrieves neuropeptide Y and cholinergic immunoreactivity in the nucleus accumbens of old rats ' (2013 ) 34 Neurobiology of Aging : 1988 -1995 .

    • Search Google Scholar

  • Pétervári, E., Balaskó, M., Garami, A., Soós, S., Székely, M. (2009) Suppression of food intake by intracerebroventricular injection of alpha-MSH varies with age in rats. Acta Physiol. Hung. 96, 483–487.

    Székely M. , 'Suppression of food intake by intracerebroventricular injection of alpha-MSH varies with age in rats ' (2009 ) 96 Acta Physiol. Hung. : 483 -487 .

    • Search Google Scholar

  • Petervari, E., Garami, A., Soos, S., Szekely, M., Balasko, M. (2010) Age-dependence of alpha-MSHinduced anorexia. Neuropeptides 44, 315–322.

    Balasko M. , 'Age-dependence of alpha-MSHinduced anorexia ' (2010 ) 44 Neuropeptides : 315 -322 .

    • Search Google Scholar

  • Porkka-Heiskanen, T., Alanko, L., Kalinchuk, A., Heiskanen, S., Stenberg, D. (2004) The effect of age on prepro-orexin gene expression and contents of orexin A and B in the rat brain. Neurobiol. Aging 25, 231–238.

    Stenberg D. , 'The effect of age on prepro-orexin gene expression and contents of orexin A and B in the rat brain ' (2004 ) 25 Neurobiol. Aging : 231 -238 .

    • Search Google Scholar

  • Smith, Y., Koylu, E. O., Couceyro, P., Kuhar, M. J. (1997) Ultrastructural localization of CART (cocaine- and amphetamine-regulated transcript) peptides in the nucleus accumbens of monkeys. Synapse 27, 90–94.

    Kuhar M. J. , 'Ultrastructural localization of CART (cocaine- and amphetamine-regulated transcript) peptides in the nucleus accumbens of monkeys ' (1997 ) 27 Synapse : 90 -94 .

    • Search Google Scholar

  • Sohn, E. H., Wolden-Hanson, T., Matsumoto, A. M. (2002) Testosterone (T)-induced changes in arcuate nucleus cocaine-amphetamine-regulated transcript and NPY mRNA are attenuated in old compared to young male brown Norway rats: contribution of T to age-related changes in cocaine-amphetamine-regulated transcript and NPY gene expression. Endocrinology 143, 954–963.

    Matsumoto A. M. , 'Testosterone (T)-induced changes in arcuate nucleus cocaine-amphetamine-regulated transcript and NPY mRNA are attenuated in old compared to young male brown Norway rats: contribution of T to age-related changes in cocaine-amphetamine-regulated transcript and NPY gene expression ' (2002 ) 143 Endocrinology : 954 -963 .

    • Search Google Scholar

  • Takano, S., Kanai, S., Hosoya, H., Ohta, M., Uematsu, H., Miyasaka, K. (2004) Orexin-A does not stimulate food intake in old rats. Am. J. Physiol. Gastrointest. Liver Physiol. 287, 1182–1187.

    Miyasaka K. , 'Orexin-A does not stimulate food intake in old rats ' (2004 ) 287 Am. J. Physiol. Gastrointest. Liver Physiol. : 1182 -1187 .

    • Search Google Scholar

  • Upadhya, M. A., Nakhate, K. T., Kokare, D. M., Singh, U., Singru, P. S., Subhedar, N. K. (2012) CART peptide in the nucleus accumbens shell acts downstream to dopamine and mediates the reward and reinforcement actions of morphine. Neuropharmacology 62, 1823–1833.

    Subhedar N. K. , 'CART peptide in the nucleus accumbens shell acts downstream to dopamine and mediates the reward and reinforcement actions of morphine ' (2012 ) 62 Neuropharmacology : 1823 -1833 .

    • Search Google Scholar

  • Vicentic, A., Dominguez, G., Hunter, R. G., Philpot, K., Wilson, M., Kuhar, M. J. (2004) Cocaine- and amphetamine-regulated transcript peptide levels in blood exhibit a diurnal rhythm: regulation by glucocorticoids. Endocrinology 145, 4119–4124.

    Kuhar M. J. , 'Cocaine- and amphetamine-regulated transcript peptide levels in blood exhibit a diurnal rhythm: regulation by glucocorticoids ' (2004 ) 145 Endocrinology : 4119 -4124 .

    • Search Google Scholar

  • Volkoff, H., Peter, R. E. (2000) Effects of CART peptides on food consumption, feeding and associated behaviors in the goldfish, Carassius auratus: actions on neuropeptide Y- and orexin A-induced feeding. Brain Res. 887, 125–133.

    Peter R. E. , 'Effects of CART peptides on food consumption, feeding and associated behaviors in the goldfish, Carassius auratus: actions on neuropeptide Y- and orexin A-induced feeding ' (2000 ) 887 Brain Res. : 125 -133 .

    • Search Google Scholar

  • Walhovd, K. B., Westlye, L. T., Amlien, I., Espeseth, T., Reinvang, I., Raz, N., Agartz, I., Salat, D. H., Greve, D. N., Fischl, B., Dale, A. M., Fjell, A. M. (2011) Consistent neuroanatomical age-related volume differences across multiple samples. Neurobiol. Aging 32, 916–932.

    Fjell A. M. , 'Consistent neuroanatomical age-related volume differences across multiple samples ' (2011 ) 32 Neurobiol. Aging : 916 -932 .

    • Search Google Scholar

  • Wolden-Hanson, T., Marck, B. T., Matsumoto, A. M. (2004) Blunted hypothalamic neuropeptide gene expression in response to fasting, but preservation of feeding responses to AgRP in aging male Brown Norway rats. Am. J. Physiol. Regul. Integr. Comp. Physiol. 287, R138–R146.

    Matsumoto A. M. , 'Blunted hypothalamic neuropeptide gene expression in response to fasting, but preservation of feeding responses to AgRP in aging male Brown Norway rats ' (2004 ) 287 Am. J. Physiol. Regul. Integr. Comp. Physiol. : R138 -R146 .

    • Search Google Scholar

  • Yang, S. C., Shieh, K. R., Li, H. Y. (2005) Cocaine- and amphetamine-regulated transcript in the nucleus accumbens participates in the regulation of feeding behavior in rats. Neuroscience 133, 841–851.

    Li H. Y. , 'Cocaine- and amphetamine-regulated transcript in the nucleus accumbens participates in the regulation of feeding behavior in rats ' (2005 ) 133 Neuroscience : 841 -851 .

    • Search Google Scholar

  • Yoshimoto, K., Kato, B., Ueda, S., Noritake, K., Sakai, K., Shibata, M., Hori, M., Kawano, H., Takeuchi, Y., Wakabayashi, Y., Yasuhara, M. (2001) Dopamine and serotonin uptake inhibitors on the release of dopamine and serotonin in the nucleus accumbens of young and aged rats. Mech. Ageing Dev. 122, 1707–1721.

    Yasuhara M. , 'Dopamine and serotonin uptake inhibitors on the release of dopamine and serotonin in the nucleus accumbens of young and aged rats ' (2001 ) 122 Mech. Ageing Dev. : 1707 -1721 .

    • Search Google Scholar
  • Collapse
  • Expand
Cover Acta Biologica Hungarica
Acta Biologica Hungarica
Print ISSN:
0236-5383
Online ISSN:
1588-256X

Senior editors

Editor(s)-in-Chief: Elekes, Károly

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

Acta Biologica Hungarica
P.O. Box 35
H-8237 Tihany, Hungary
Phone: (36 87) 448 244 ext. 103
Fax: (36 87) 448 006
E-mail: elekes@tres.blki.hu

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture
  • Biology and Environmental Sciences
  • Elsevier BIOBASE
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Acta Biologica Hungarica
Language English
Size  
Year of
Foundation		 1950
Publication
Programme		 changed title
Volumes
per Year		  
Issues
per Year		  
Founder Magyar Tudományos Akadémia
Founder's
Address		 H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Mar 2024 29 0 0
Apr 2024 29 0 0
May 2024 26 0 0
Jun 2024 18 0 0
Jul 2024 37 0 0
Aug 2024 40 1 0
Sep 2024 3 0 0