View More View Less
  • 1 University of Debrecen, Debrecen, Hungary
Restricted access

Relative transcriptions of Aspergillus nidulans dug1-3 (orthologes of Saccharomyces cerevisiae DUG — deficient in utilization of glutathione — pathway genes) and ggtA encoding γ-glutamyl transpeptidase were studied under conditions inducing glutathione degradation. GgtA was induced in all cases when glutathione levels decreased, but addition of yeast extract, which moderated glutathione degradation, enhanced its induction. Although dug2 showed constitutive transcription, dug1 and dug3 were induced by carbon and nitrogen starvation and yeast extract did not caused significant changes in their relative transcription. The in silico reconstructed DUG pathway of A. nidulans is a promising candidate for cytosolic GSH degradation induced by carbon/nitrogen stress.

  • 1.

    Adamis, P. D., Mannarino, S. C., Eleutherio, E. C. (2009) Glutathione and gamma-glutamyl transferases are involved in the formation of cadmium-glutathione complex. FEBS Lett. 583, 14891492.

    • Search Google Scholar
    • Export Citation
  • 2.

    Bello, M. H., Morin, D., Epstein, L. (2013) γ-Glutamyltransferases (GGT) in Colletotrichum graminicola: mRNA and enzyme activity, and evidence that CgGGT1 allows glutathione utilization during nitrogen deficiency. Fungal Genet. Biol. 51, 7283.

    • Search Google Scholar
    • Export Citation
  • 3.

    Heisterkamp, N., Groffen, J., Warburton, D., Sneddon, T. P. (2008) The human gamma-glutamyltransferase gene family. Hum. Genet. 123, 321332.

    • Search Google Scholar
    • Export Citation
  • 4.

    Kaur, H., Ganguli, D., Bachhawat, A. K. (2012) Glutathione degradation by the alternative pathway (DUG pathway) in Saccharomyces cerevisiae is initiated by (Dug2p-Dug3p)2 complex, a novel glutamine amidotransferase (GATase) enzyme acting on glutathione. J. Biol. Chem. 287, 89208931.

    • Search Google Scholar
    • Export Citation
  • 5.

    Kimura, K., Tran, L. S., Uchida, I., Itoh, Y. (2004) Characterization of Bacillus subtilis γ-glutamyltranspeptidase and its involvement in the degradation of capsule poly-gammaglutamate. Microbiol. 150, 41154123.

    • Search Google Scholar
    • Export Citation
  • 6.

    Kumar, A., Tikoo, S., Maity, S., Sengupta, S., Sengupta, S., Kaur, A., Bachhawat, A. K. (2012) Mammalian proapoptotic factor ChaC1 and its homologues function as γ-glutamyl cyclotransferases acting specifically on glutathione. EMBO Rep. 13, 10951101.

    • Search Google Scholar
    • Export Citation
  • 7.

    Ohkama-Ohtsu, N., Oikawa, A., Zhao, P., Xiang, C., Saito, K., Oliver, D. J. (2008) A gamma-glutamyl transpeptidase-independent pathway of glutathione catabolism to glutamate via 5-oxoproline in Arabidopsis. Plant Physiol. 148, 16031613.

    • Search Google Scholar
    • Export Citation
  • 8.

    Pócsi, I., Prade, R. A., Penninckx, M. J. (2004) Glutathione, altruistic metabolite in fungi. Adv.Microb. Physiol. 49, 176.

  • 9.

    Shibayama, K., Wachino, J., Arakawa, Y., Saidijam, M., Rutherford, N. G., Henderson, P. J. (2007) Metabolism of glutamine and glutathione via gamma-glutamyltranspeptidase and glutamate transport in Helicobacter pylori: possible significance in the pathophysiology of the organism. Mol. Microbiol. 64, 396406.

    • Search Google Scholar
    • Export Citation
  • 10.

    Szilágyi, M., Miskei, M., Karányi, Z., Lenkey, B., Pócsi, I., Emri, T. (2013) Transcriptome changes initiated by carbon starvation in Aspergillus nidulans. Microbiology 159, 176190.

    • Search Google Scholar
    • Export Citation

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

Acta Biologica Hungarica
P.O. Box 35
H-8237 Tihany, Hungary
Phone: (36 87) 448 244 ext. 103
Fax: (36 87) 448 006
E-mail: elekes@tres.blki.hu

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture
  • Biology and Environmental Sciences
  • Elsevier BIOBASE
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Acta Biologica Hungarica
Language English
Size  
Year of
Foundation
1950
Publication
Programme
changed title
Volumes
per Year
 
Issues
per Year
 
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)