Authors:
Nikolett Baranyi University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary

Search for other papers by Nikolett Baranyi in
Current site
Google Scholar
PubMed
Close
,
Daniela Jakšić Despot University of Zagreb, Schrottova 39, 10000 Zagreb, Croatia

Search for other papers by Daniela Jakšić Despot in
Current site
Google Scholar
PubMed
Close
,
Andrea Palágyi University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary

Search for other papers by Andrea Palágyi in
Current site
Google Scholar
PubMed
Close
,
Noémi Kiss University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary

Search for other papers by Noémi Kiss in
Current site
Google Scholar
PubMed
Close
,
Sándor Kocsubé University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary

Search for other papers by Sándor Kocsubé in
Current site
Google Scholar
PubMed
Close
,
András Szekeres University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary

Search for other papers by András Szekeres in
Current site
Google Scholar
PubMed
Close
,
Anita Kecskeméti University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary

Search for other papers by Anita Kecskeméti in
Current site
Google Scholar
PubMed
Close
,
Ottó Bencsik University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary

Search for other papers by Ottó Bencsik in
Current site
Google Scholar
PubMed
Close
,
Csaba Vágvölgyi University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary

Search for other papers by Csaba Vágvölgyi in
Current site
Google Scholar
PubMed
Close
,
Maja Šegvić Klarić University of Zagreb, Schrottova 39, 10000 Zagreb, Croatia

Search for other papers by Maja Šegvić Klarić in
Current site
Google Scholar
PubMed
Close
, and
János Varga University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary

Search for other papers by János Varga in
Current site
Google Scholar
PubMed
Close
Restricted access

The occurrence of potential aflatoxin producing fungi was examined in various agricultural products and indoor air in Central European countries including Hungary, Serbia and Croatia. For species identification, both morphological and sequence based methods were applied. Aspergillus flavus was detected in several samples including maize, cheese, nuts, spices and indoor air, and several isolates were able to produce aflatoxins. Besides, three other species of Aspergillus section Flavi, A. nomius, A. pseudonomius and A. parasiticus were also isolated from cheese, maize and indoor air, respectively. This is the first report on the occurrence of A. nomius and A. pseudonomius in Central Europe. All A. nomius, A. pseudonomius and A. parasiticus isolates were able to produce aflatoxins B1, B2, G1 and G2. The A. nomius isolate came from cheese produced very high amounts of aflatoxins (above 1 mg ml–1). All A. nomius, A. pseudonomius and A. parasiticus isolates produced much higher amounts of aflatoxin G1 then aflatoxin B1. Further studies are in progress to examine the occurrence of producers of these highly carcinogenic mycotoxins in agricultural products and indoor air in Central Europe.

  • 1.

    Altschul, S. F., Gish, W., Miller, W., Myers, E. W., Lipman, D. J. (1990) Basic local alignment search tool. J. Mol. Biol. 215, 403410.

    • Search Google Scholar
    • Export Citation
  • 2.

    Baquião, A. C., de Oliveira, M. M., Reis, T. A., Zorzete, P., Diniz Atayde, D., Correa, B. (2013) Polyphasic approach to the identification of Aspergillus section Flavi isolated from Brazil nuts. FoodChem. 139, 11271132.

    • Search Google Scholar
    • Export Citation
  • 3.

    Baranyi, N., Kocsubé, S., Vágvölgyi, C., Varga, J. (2013) Current trends in aflatoxin research. Acta Biol. Szeged. 57, 95107.

  • 4.

    Caira, M., Posteraro, B., Sanguinetti, M., de Carolis, E., Leone, G., Pagano, L. (2012) First case of breakthrough pneumonia due to Aspergillus nomius in a patient with acute myeloid leukemia. Med. Mycol. 50, 746750.

    • Search Google Scholar
    • Export Citation
  • 5.

    Calderari, T. O., Iamanaka, B. T., Frisvad, J. C., Pitt, J. I., Sartori, D., Pereira, J. L., Fungaro, M. H., Taniwaki, M. H. (2013) The biodiversity of Aspergillus section Flavi in brazil nuts: from rainforest to consumer. Int. J. Food Microbiol. 160, 267272.

    • Search Google Scholar
    • Export Citation
  • 6.

    Carbone, I., Jakobek, J. L., Ramirez-Prado, J. H., Horn, B. W. (2007) Recombination, balancing selection and adaptive evolution in the aflatoxin gene cluster of Aspergillus parasiticus. Mol. Ecol. 16, 44014417.

    • Search Google Scholar
    • Export Citation
  • 7.

    Copetti, M. V., Iamanaka, B. T., Pereira, J. L., Fungaro, M. H., Taniwaki, M. H. (2011) Aflatoxigenic fungi and aflatoxin in cocoa. Int. J. Food Microbiol. 148, 141144.

    • Search Google Scholar
    • Export Citation
  • 8.

    Cvetnić, Z., Pepeljnjak, S. (1995) Aflatoxin producing potential of Aspergillus flavus and A. parasiticus isolated from samples of smoked dried meat. Die Nahrung 39, 302307.

    • Search Google Scholar
    • Export Citation
  • 9.

    de Hoog, G. S., Guarro, J., Gene, J., Figueras, M. J. (2000) Atlas of Clinical Fungi. 2nd ed. Centraalbureau voor Schimmelcultures, Utrecht/Universitat Rovira i Virgili, Reus.

    • Search Google Scholar
    • Export Citation
  • 10.

    Dovičičová, M., Tančinová, D., Labuda, R., Sulyok, M. (2012) Aspergillus parasiticus from wheat grain of Slovak origin and its toxigenic potency. Czech J. Food Sci. 30, 483487.

    • Search Google Scholar
    • Export Citation
  • 11.

    Ehrlich, K. C., Kobbeman, K., Montalbano, B. G., Cotty, P. J. (2007) Aflatoxin-producing Aspergillus species from Thailand. Int. J. Food Microbiol. 114, 153159.

    • Search Google Scholar
    • Export Citation
  • 12.

    Fazekas, B., Tar, A., Kovacs, M. (2005) Aflatoxin and ochratoxin A content of spices in Hungary. Food Addit. Contam. 22, 856863.

  • 13.

    Foley, K., Fazio, G., Jensen, A. B., Hughes, W. O. (2014) The distribution of Aspergillus spp. Opportunistic parasites in hives and their pathogenicity to honey bees. Vet. Microbiol. 169, 203210.

    • Search Google Scholar
    • Export Citation
  • 14.

    Gonçalves, J. S., Ferracin, L. M., Carneiro Vieira, M. L., Iamanaka, B. T., Taniwaki, M. H., Pelegrinelli Fungaro, M. H. (2012) Molecular analysis of Aspergillus section Flavi isolated from Brazil nuts. World J. Microbiol. Biotechnol. 28, 18171825.

    • Search Google Scholar
    • Export Citation
  • 15.

    Hong, S. B., Cho, H. S., Shin, H. D., Frisvad, J. C., Samson, R. A. (2006) Novel Neosartorya species isolated from soil in Korea. Int. J. Syst. Evol. Microbiol. 56, 477486.

    • Search Google Scholar
    • Export Citation
  • 16.

    Horn, B. W., Moore, G. G., Carbone, I. (2011) Sexual reproduction in aflatoxin-producing Aspergillus nomius. Mycologia 103, 174183.

  • 17.

    Kenjo, T., Ishide, Y., Aoyama, K., Ichinoe, M. (2007) Fungal population and distribution of aflatoxigenic fungi in commercial almond powder products. Shokuhin Eiseigaku Zasshi 48, 9096. (in Japanese)

    • Search Google Scholar
    • Export Citation
  • 18.

    Kensler, T. W., Roebuck, B. D., Wogan, G. N., Groopman, J. D. (2011) Aflatoxin: a 50-year odyssey of mechanistic and translational toxicology. J. Toxicol. Sci. 120, S28–S48.

    • Search Google Scholar
    • Export Citation
  • 19.

    King, A. D. Jr., Hocking, A. D., Pitt, J. I. (1979) Dichloran-rose bengal medium for enumeration and isolation of molds from foods. Appl. Environ. Microbiol. 37, 959964.

    • Search Google Scholar
    • Export Citation
  • 20.

    Kumeda, Y., Asao, T., Takahashi, H., Ichinoe, M. (2003) High prevalence of B and G aflatoxin producing fungi in sugarcane fields in Japan: heteroduplex panel analysis identifies a new genotype within Aspergillus section Flavi and Aspergillus nomius. FEMS Microbiol. Ecol. 45, 229238.

    • Search Google Scholar
    • Export Citation
  • 21.

    Kurtzman, C. P., Horn, B. W., Hesseltine, C. W. (1987) Aspergillus nomius, a new aflatoxin-producing species related to Aspergillus flavus and Aspergillus tamarii. Antonie Van Leeuwenhoek 53, 147158.

    • Search Google Scholar
    • Export Citation
  • 22.

    Manikandan, P., Varga, J., Kocsubé, S., Samson, R. A., Anita, R., Revathi, R., Dóczi, I., Németh, T. M., Narendran, V., Vágvölgyi, C., Manoharan, C., Kredics, L. (2009) Mycotic keratitis due to Aspergillus nomius. J. Clin. Microbiol. 47, 33823385.

    • Search Google Scholar
    • Export Citation
  • 23.

    Massi, F. P., Vieira, M. L., Sartori, D., Penha, R. E., de Freitas Munhoz, C., Ferreira, J. M., Iamanaka, B. T., Taniwaki, M. H., Frisvad, J. C., Fungaro, M. H. (2014) Brazil nuts are subject to infection with B and G aflatoxin-producing fungus, Aspergillus pseudonomius. Int. J. Food Microbiol. 186, 1421.

    • Search Google Scholar
    • Export Citation
  • 24.

    Matumba, L., Sulyok, M., Njoroge, S. M., Njumbe Ediage, E., Van Poucke, C., De Saeger, S., Krska, R. (2014) Uncommon occurrence ratios of aflatoxin B1, B2, G1, and G2 in maize and groundnuts from Malawi. Mycotoxin Res. 31, 5762.

    • Search Google Scholar
    • Export Citation
  • 25.

    Okano, K., Tomita, T., Ohzu, Y., Takai, M., Ose, A., Kotsuka, A., Ikeda, N., Sakata, J., Kumeda, Y., Nakamura, N., Ichinoe, M. (2012) Aflatoxins B and G contamination and aflatoxigenic fungi in nutmeg. Shokuhin Eiseigaku Zasshi 53, 211216.

    • Search Google Scholar
    • Export Citation
  • 26.

    Olsen, M., Johnsson, P., Möller, T., Paladino, R., Lindblad, M. (2008) Aspergillus nomius, an important aflatoxin producer in Brazil nuts? World Mycotoxin J. 1, 123126.

    • Search Google Scholar
    • Export Citation
  • 27.

    Pepeljnjak, S., Šegvić, M. (2004) An overview of mycotoxins and toxigenic fungi in Croatia. In: Logrieco, A., Visconti, A. (eds) An Overview on Toxigenic Fungi and Mycotoxins in Europe. Kluwer Academic Publishers, Dordrecht, pp. 3350.

    • Search Google Scholar
    • Export Citation
  • 28.

    Pitt, J. I., Hocking, A. D. (2009) Fungi and Food Spoilage. Springer Science+Business Media LLC, New York (NY).

  • 29.

    Pitt, J. I., Hocking, A. D., Bhudhasamai, K., Miscamble, B. F., Wheeler, K. A., Tanboon-Ek, P. (1993) The normal mycoflora of commodities from Thailand. 1. Nuts and oilseeds. Int. J. Food Microbiol. 20, 211226.

    • Search Google Scholar
    • Export Citation
  • 30.

    Pitt, J. I., Hocking, A. D., Bhudhasamai, K., Miscamble, B. F., Wheeler, K. A., Tanboon-Ek, P. (1994) The normal mycoflora of commodities from Thailand. 2. Beans, rice, small grains and other commodities. Int. J. Food Microbiol. 23, 3553.

    • Search Google Scholar
    • Export Citation
  • 31.

    Raper, K. B., Fennell, D. I. (1965) The Genus Aspergillus. Williams & Wilkins, Baltimore, US.

  • 32.

    Razzaghi-Abyaneh, M., Shams-Ghahfarokhi, M., Allameh, A., Kazeroon-Shiri, A., Ranjbar-Bahadori, S., Mirzahoseini, H., Rezaee, M. B. (2006) A survey on distribution of Aspergillus section Flavi in corn field soils in Iran: population patterns based on aflatoxins, cyclopiazonic acid and sclerotia production. Mycopathologia 161, 183192. Acta Biologica Hungarica

    • Search Google Scholar
    • Export Citation
  • 33.

    Reis, T. A., Baquião, A. C., Atayde, D. D., Grabarz, F., Corrêa, B. (2014) Characterization of Aspergillus section Flavi isolated from organic Brazil nuts using a polyphasic approach. Food Microbiol. 42, 3439.

    • Search Google Scholar
    • Export Citation
  • 34.

    Samson, R. A., Hoekstra, E. S., Lund, F., Filtenborg, O., Frisvad, J. C. (2004) Introduction to Foodand Airborne Fungi. CBS Fungal Biodiversity Center, Utrecht, Netherlands.

    • Search Google Scholar
    • Export Citation
  • 35.

    Schmidt-Heydt, M., Rüfer, C. E., Abdel-Hadi, A., Magan, N., Geisen, R. (2010) The production of aflatoxin B1 or G1 by Aspergillus parasiticus at various combinations of temperature and water activity is related to the ratio of aflS to aflR expression. Mycotoxin Res. 26, 241246.

    • Search Google Scholar
    • Export Citation
  • 36.

    Sebők, F., Dobolyi, C., Hartman, M., Risa, A., Kritafon, C., Szoboszlay, S., Kriszt, B. (2014) Occurrence of potentially aflatoxin producing Aspergillus species in maize fields of Hungary. In: Abstract Book of the Meeting of the Hungarian Microbiological Society and the EU FP7 PROMISERegional Meeting, pp. 6263.

    • Search Google Scholar
    • Export Citation
  • 37.

    Smedsgaard, J. (1997) Micro-scale extraction procedure for standardized screening of fungal metabolite production in cultures. J. Chromatogr. A 760, 264270.

    • Search Google Scholar
    • Export Citation
  • 38.

    Stubblefield, R. D., Shotwell, O. L. (1977) Reverse phase analytical and preparative high pressure liquid chromatography of aflatoxins. J. Assoc. Off. Anal. Chem. 60, 784790.

    • Search Google Scholar
    • Export Citation
  • 39.

    Tam, E. W., Chen, J. H., Lau, E. C., Ngan, A. H., Fung, K. S., Lee, K. C., Lam, C. W., Yuen, K. Y., Lau, S. K., Woo, P. C. (2014) Misidentification of Aspergillus nomius and Aspergillus tamarii as Aspergillus flavus: characterization by internal transcribed spacer, β-tubulin, and calmodulin gene sequencing, metabolic fingerprinting, and matrix-assisted laser desorption ionization-time of flight mass spectrometry. J. Clin. Microbiol. 52, 11531160.

    • Search Google Scholar
    • Export Citation
  • 40.

    Varga, J., Frisvad, J. C., Samson, R. A. (2009) A reappraisal of fungi producing aflatoxins. World Mycotoxin J. 2, 263277.

  • 41.

    Varga, J., Frisvad, J. C., Samson, R. A. (2011) Two new aflatoxin producing species, and an overview of Aspergillus section Flavi. Stud. Mycol. 69, 5780.

    • Search Google Scholar
    • Export Citation
  • 42.

    Varga, J., Kerepes, L., Kocsubé, S., Szigeti, G., Baranyi, N., Vágvölgyi, C. (2013) Examination of the diversity of indoor molds in a Hungarian student hostel. Acta Biol. Szeged. 57, 2124.

    • Search Google Scholar
    • Export Citation
  • 43.

    Visagie, C. M., Hirooka, Y., Tanney, J. B., Whitfield, E., Mwange, K., Meijer, M., Amend, A. S., Seifert, K. A., Samson, R. A. (2014) Aspergillus, Penicillium and Talaromyces isolated from house dust samples collected around the world. Stud. Mycol. 78, 63139.

    • Search Google Scholar
    • Export Citation
  • 44.

    Zotti, M., Machetti, M., Persi, A., Barabino, G., Parodi, A. (2011) Onychomycosis: first case due to Aspergillus nomius. Acta Derm. Venereol. 91, 591592.

    • Search Google Scholar
    • Export Citation
  • Collapse
  • Expand

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

Acta Biologica Hungarica
P.O. Box 35
H-8237 Tihany, Hungary
Phone: (36 87) 448 244 ext. 103
Fax: (36 87) 448 006
E-mail: elekes@tres.blki.hu

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture
  • Biology and Environmental Sciences
  • Elsevier BIOBASE
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Acta Biologica Hungarica
Language English
Size  
Year of
Foundation
1950
Publication
Programme
changed title
Volumes
per Year
 
Issues
per Year
 
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)