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  • 1 Siedlce University of Natural Sciences and Humanities, 08-110 Siedlce, Prusa 12, Poland
  • 2 Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10, 10-747 Olsztyn, Poland
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The impact of short-term UV-B treatment on the content of individual flavonoids and photosynthetic pigments in cotyledons and the growth of common buckwheat (Fagopyrum esculentum Moench) seedlings was investigated. Seeds of four common buckwheat cultivars were germinated in darkness over a period of 4 days and acclimatized for 2 days under a 16/8 h light/dark photoperiod at 24/18 °C day/night, and exposure to 100–120 μmol ∙ m−2 ∙ s−1 of photosynthetically active radiation (PAR). Seedlings were divided into three batches, including two batches subjected to different doses of UV-B (5 W ∙ m−2 and 10 W ∙ m−2, one hour per day) for 5 days, and a control group exposed to PAR only. Exposure to UV-B increased anthocyanin levels in the cotyledons of all examined cultivars, it inhibited hypocotyl elongation, but did not affect the content of photosynthetic pigments. Flavone concentrations increased in cv. Red Corolla and Kora, remained constant in cv. Panda and decreased in cv. Hruszowska. Exposure to UV-B decreased rutin levels in cv. Hruszowska, but not in the remaining cultivars. Cultivars Hruszowska, Panda and Kora appeared to be less resistant to UV-B than Red Corolla. Higher resistance to UV-B radiation in Red Corolla can probably be attributed to its higher content of anthocyanins and rutin in comparison with the remaining cultivars.

  • 1.

    Agati, G., Tattini, M. (2010) Multiple functional roles of flavonoids in photoprotection. New Phytol. 186, 786793.

  • 2.

    Bandurska, H., Pietrowska-Borek, M., Cieslak, M. (2012) Response of barley seedlings to water deficit and enhanced UV-B irradiation acting alone and in combination. Acta Physiol. Plant. 34, 161171.

    • Search Google Scholar
    • Export Citation
  • 3.

    Burchard, P., Bilger, W., Weissenböck, G. (2000) Contribution of hydroxycinnamates and flavonoids to epidermal shielding of UV-A and UV-B radiation in developing rye primary leaves as measured by ultraviolet-induced chlorophyll fluorescence measurements. Plant Cell Environ. 23, 13731380.

    • Search Google Scholar
    • Export Citation
  • 4.

    Cechin, I., Fumis, T. F., Dokkedal, A. L. (2007) Growth and physiological responses of sunflower plants exposed to ultraviolet-B radiation. Cienc. Rur. 37, 8590.

    • Search Google Scholar
    • Export Citation
  • 5.

    Correia, C. M., Areal, E. L. V., Torres-Pereira, M. S., Torres-Pereira, J. M. G. (1998) Intraspecific variation in sensitivity to ultraviolet-B radiation in maize grown under field conditions. I. Growth and morphological aspects. Field Crops Res. 59, 8189.

    • Search Google Scholar
    • Export Citation
  • 6.

    Dai, Q. J., Peng, S. B., Chavez, A. Q., Vergara, B. S. (1994) Intraspecific responses of 188 rice cultivars to enhanced UV-B radiation. Environ. Exp. Bot. 34, 422433.

    • Search Google Scholar
    • Export Citation
  • 7.

    Eguchi, K., Sato, T. (2009) Differences in the ratios of cyanidin-3-O-glucoside and cyanidin-3-Orutinoside to total anthocyanin under UV and non-UV conditions in Tartary buckwheat (Fagopyrum tataricum Gaertn). Plant Prod. Sci. 12, 150155.

    • Search Google Scholar
    • Export Citation
  • 8.

    Eichholz, I., Rohn, S., Gamm, A., Beesk, N., Herppich, W. B., Kroh, L. W., Ulrichs C., Huyskens-Keil, S. (2012) UV-B-mediated flavonoid synthesis in white asparagus (Asparagus officinalis L.). Food Res. Int. 48, 196201.

    • Search Google Scholar
    • Export Citation
  • 9.

    El Morchid, E. M., Londoño, P. T., Papagiannopoulos, M., Gobbo-Neto, L., Müller, C. (2014) Variation in flavonoid pattern in leaves and flowers of Primula veris of different origin and impact of UV-B. Biochem. Syst. Ecol. 53, 8188.

    • Search Google Scholar
    • Export Citation
  • 10.

    Fiscus, E. L., Philbeck, R., Britt, A. B., Booker, F. L. (1999) Growth of Arabidopsis flavonoid mutants under solar radiation and UV filters. Environ. Exp. Bot. 41, 231245.

    • Search Google Scholar
    • Export Citation
  • 11.

    Gaberšcik, A., Voncina, M., Trost, T., Germ, M., Björn, L. O. (2002) Growth and production of buckwheat (Fagopyrum esculentum) treated with reduced, ambient, and enhanced UV-B radiation. J. Photoch. Photobio. B. 66, 3036.

    • Search Google Scholar
    • Export Citation
  • 12.

    Germ, M., Breznik, B. (2007) Responses of common buckwheat (Fagopyrum esculentum) to enhanced UV-B radiation and selenium. Proceedings of the 10th International Symposium on Buckwheat, August 14–18. 2007, Northwest A.&F. University Press, Yangling, Shaanx; Prov., P. R. China. pp. 127130.

    • Search Google Scholar
    • Export Citation
  • 13.

    Gould, K. S. (2004) Nature’s Swiss army knife: The diverse protective roles of anthocyanins in leaves. J. Biomed. Biotechnol. 5, 314320.

    • Search Google Scholar
    • Export Citation
  • 14.

    Horbowicz, M., Wiczkowski, W., Koczkodaj, D., Saniewski, M. (2011) Effects of methyl jasmonate on accumulation of flavonoids in seedlings of common buckwheat (Fagopyrum esculentum Moench). Acta Biol. Hung. 62, 265278.

    • Search Google Scholar
    • Export Citation
  • 15.

    Jansen, M. A. K., van den Noort, R. E., Tan, M. Y. A., Prinsen, E., Lagrimini, L. M., Thorneley, R. N. F. (2001) Phenol-oxidizing peroxidases contribute to the protection of plants from ultraviolet-B radiation stress. Plant Physiol. 126, 10121023.

    • Search Google Scholar
    • Export Citation
  • 16.

    Kreft, S., Štrukelj, B., Gaberšcik, A., Kreft, I. (2002) Rutin in buckwheat herbs grown at different UV-B radiation levels: comparison of two UV spectrophotometric and an HPLC method. J. Exp. Bot. 53, 18011804.

    • Search Google Scholar
    • Export Citation
  • 17.

    Landi, M., Tattini, M., Gould, K. S. (2015) Multiple functional roles of anthocyanins in plant-environment interactions. Environ. Exp. Bot. 119, 417.

    • Search Google Scholar
    • Export Citation
  • 18.

    Lichtenthaler, H. K., Wellburn, A. R. (1985) Determination of total carotenoids and chlorophylls A and B of leaf in different solvents. Bioch. Soc. Trans. 11, 591592.

    • Search Google Scholar
    • Export Citation
  • 19.

    Mewis, I., Schreiner, M., Nguyen, C.N., Krumbein, A., Ulrichs, C., Lohse, M., Zrenner, R. (2012) UV-B irradiation changes specifically the secondary metabolite profile in broccoli sprouts –Induced signaling overlaps with defense response to biotic stressors. Plant Cell Physiol. 53, 15461560.

    • Search Google Scholar
    • Export Citation
  • 20.

    Müller, V., Lankes, C., Albert, A., Winkler, J. B., Zimmermann, B. F., Noga, G., Hunsche, M. (2015) Concentration of hinokinin, phenolic acids and flavonols in leaves and stems of Hydrocotyle leucocephala is differently influenced by PAR and ecologically relevant UV-B level. J. Plant Physiol. 173, 105115.

    • Search Google Scholar
    • Export Citation
  • 21.

    Nascimento, L. B., Leal-Costa, M. V., Menezes, E. A., Lopes, V. R., Muzitano, M. F., Costa, S. S., Tavares, E. S. (2015) Ultraviolet-B radiation effects on phenolic profile and flavonoid content of Kalanchoe pinnata. J. Photoch. Photobio. B. 148, 7381.

    • Search Google Scholar
    • Export Citation
  • 22.

    Neugart, S., Zietz, M., Schreiner, M., Rohn, S., Kroh, L. W., Krumbein, A. (2012) Structurally different flavonol glycosides and hydroxycinnamic acid derivatives respond differently to moderate UV-B radiation exposure. Physiol. Plant. 145, 582593.

    • Search Google Scholar
    • Export Citation
  • 23.

    Pollastri, S., Tattini, M. (2011) Flavonols: old compounds for old roles. Ann. Bot. 108, 12251233.

  • 24.

    Regvar, M., Bukovnik, U., Likar, M., Kreft, I. (2012) UV-B radiation affects flavonoids and fungal colonisation in Fagopyrum esculentum and F. tataricum. Cent. Eur. J. Biol. 7, 275283.

    • Search Google Scholar
    • Export Citation
  • 25.

    Suzuki, T., Honda, Y., Mukasa, Y. (2005) Effects of UV-B radiation, cold and desiccation stress on rutin concentration and rutin glucosidase activity in Tartary buckwheat (Fagopyrum tataricum) leaves. Plant Sci. 168, 13031307.

    • Search Google Scholar
    • Export Citation
  • 26.

    Takahama, U. (2004) Oxidation of vacuolar and apoplastic phenolic substrates by peroxidase: physiological significance of the oxidation reactions. Phytochem. Rev. 3, 207219.

    • Search Google Scholar
    • Export Citation
  • 27.

    Tevini, M., Braun, J., Fieser, G. (1991) The productive function of the epidermal layer of rye seedling against ultraviolet-B radiation. Photoch. Photobio. 53, 329333.

    • Search Google Scholar
    • Export Citation
  • 28.

    Tsurunaga, Y., Takahashi, T., Katsube, T., Kudo, A., Kuramitsu, O., Ishiwata, M., Matsumoto, S. (2013) Effects of UV-B irradiation on the levels of anthocyanin, rutin and radical scavenging activity of buckwheat sprouts. Food Chem. 14, 552556.

    • Search Google Scholar
    • Export Citation
  • 29.

    Wiczkowski, W., Szawara-Nowak, D., Debski, H., Mitrus J., Horbowicz M. (2014) Comparison of flavonoids profile in sprouts of common buckwheat cultivars and wild Tartary buckwheat. Int. J. Food Sci. Tech. 49, 19771984.

    • Search Google Scholar
    • Export Citation
  • 30.

    Yao, Y., Yang, Y., Lutts, S. (2008) Intraspecific responses of Fagopyrum esculentum to enhanced ultraviolet B radiation. Plant Growth Regul. 56, 297306.

    • Search Google Scholar
    • Export Citation
  • 31.

    Yao, Y. N., Xuan, Z. Y., Li, Y. A., He, Y. M., Korpelainen H., Li, C. Y. (2006) Effects of ultraviolet-B radiation on crop growth, development, yield and leaf pigment concentration of Tartary buckwheat (Fagopyrum tataricum) under field conditions. Europ. J. Agron 25, 215222.

    • Search Google Scholar
    • Export Citation
  • 32.

    Yunqun, Z., Yuan, L., Haiyan, C., Jianjun, C. (2003) Intraspecific differences in physiological response of 20 soybean cultivars to enhanced ultraviolet-B radiation under field conditions. Environ. Exp. Bot. 50, 8797.

    • Search Google Scholar
    • Export Citation
  • 33.

    Zhang, W. J., Björn, L. O. (2009) The effect of ultraviolet radiation on the accumulation of medicinal compounds in plants. Fitoterapia 80, 207218.

    • Search Google Scholar
    • Export Citation