View More View Less
  • 1 Ain Shams University, El Makres St. Roxy, Cairo, Egypt
Restricted access

The present study was undertaken to examine the possible roles of sodium nitroprusside in protection against oxidative damage due to zinc toxicity in sunflower plants. Physiochemical parameters in sunflower plants exposed to Zn2+ (100, 200 and 300 mg/kg soil) alone or combined with SNP were measured. The results showed that excess of Zn decreased plant growth, seed yield components and photosynthetic pigments content. On the other hand, Zn stress increased the level of non-enzymatic antioxidants (ascorbic acid and reduced glutathione) and enzymatic antioxidants (superoxide dismutase, ascrobate peroxidase and glutathione reductase), coupled with the appearance of novel protein bands. Furthermore, Zn stress increased Zn content in roots and shoots. The amounts of Zn in roots were higher than shoots. A marked increase in total saturated fatty acids accompanied by a decrease in total unsaturated fatty acids was observed. Exogenously application of SNP (20 μM) increased growth parameters, photosynthetic pigments content, ascorbic acid and glutathione contents, antioxidant enzyme activities and the quality of the oil in favour of the increase of unsaturated fatty acids. Moreover, SNP application increased Zn concentration in roots and inhibited Zn accumulation in shoots. Therefore, it is concluded that SNP treatment can help reduce Zn toxicity in sunflower plants.

  • 1.

    A.O.A.C. (1990) Official Methods of Analysis. 20th edition. Association of Official Analytical Chemists, Arlington, Virginia, U.S.A.

  • 2.

    A.O.A.C. (2005) Association of Official Agricultural Chemists. In: Hortwitz, W., Latimer, G. W. (ed.) Official Methods of Analysis. 18th. Gaithersburg

    • Search Google Scholar
    • Export Citation
  • 3.

    Aguilar-Alonso, P., Martinez-Fong, D., Pazos-Salazar, N. G., Brambila, E. et al. (2008) The increase in zinc levels and upregulation of zinc transporters are mediated by nitric oxide in the cerebral cortex after transient ischemia in the rat. Brain Res. 1200, 8998.

    • Search Google Scholar
    • Export Citation
  • 4.

    Ali, Q., Ashraf, M., Anwar, F. (2009) Physico-chemical attributes of seed oil from drought stressed sunflower (Helianthus annuus L.) plants. Grasas y Aceites 60, 475481.

    • Search Google Scholar
    • Export Citation
  • 5.

    Apel, K., Hirt, H. (2004) Reactive oxygen species: metabolism, oxidative stress, and signaling transduction. Annu. Rev. Plant Biol. 55, 373399.

    • Search Google Scholar
    • Export Citation
  • 6.

    Beauchamp, C., Fridovich, I. (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochem. 44, 276287.

    • Search Google Scholar
    • Export Citation
  • 7.

    Ciftci-Yilmaz, S., Mittler, R. (2008) The zinc finger network of plants. Cell Mol. Life Sci. 65, 11501160.

  • 8.

    Dong, Y., Xua, L., Wanga Q., Fana, Z., Konga, J., Baia, X. (2014) Effects of exogenous nitric oxide on photosynthesis, antioxidative ability, and mineral element contents of perennial ryegrass under copper stress. J. Plant Interact. 9, 402411.

    • Search Google Scholar
    • Export Citation
  • 9.

    Esim, N., Atici, O. (2014) Nitric oxide improves chilling tolerance of maize by affecting apoplastic antioxidative enzymes in leaves. Plant Growth Regul. 72, 2938.

    • Search Google Scholar
    • Export Citation
  • 10.

    Foyer, C. H., Halliwell, B. (1976) The presence of glutathione and glutathione reductase in chloroplasts: A proposed role in ascorbic acid metabolism. Planta 133, 2125.

    • Search Google Scholar
    • Export Citation
  • 11.

    Foyer, C. H., Noctor, G. (2005) Oxidant and antioxidant signalling in plants: Are evaluation of the concept of oxidative stress in a physiological context. Plant Cell and Environ. 28, 10561071.

    • Search Google Scholar
    • Export Citation
  • 12.

    Gomez, K. A., Gomez, A. A. (1984) Statistical Analysis Procedures for Agricultural Research. John Wiley and Sons, New York, NY, USA. 2530.

    • Search Google Scholar
    • Export Citation
  • 13.

    Grün, S., Lindermayr, C., Sell, S., Durner, J. (2006) Nitric oxide and gene regulation in plants. J. Exp. Bot. 57, 507516.

  • 14.

    Harborne, J. B. (1984) Phytochemical methods: A guide to modern techniques of plant analysis. 2nd Edition, London, N. Y., 15. p.

  • 15.

    Hissin, P. J., Hilf, R. (1976) A fluorometric method for determination of oxidized and reduced glutathione in tissues. Ana. Biochem. 74, 214226.

    • Search Google Scholar
    • Export Citation
  • 16.

    Hossain, Z., Nouri, M. Z., Komatsu, S. (2012) Plant cell organelle proteomics in response to abiotic stress. J. Proteome Res. 11, 3748.

    • Search Google Scholar
    • Export Citation
  • 17.

    Inbaraj, M. P., Krishnaswamy, M. (2011) Photoinhibition of photosynthesis in leaves of cowpea (Vigna unguiculata L.Walp.P152) in response to zinc and high irradiance stresses. World Applied Sci. 15, 13341343.

    • Search Google Scholar
    • Export Citation
  • 18.

    Jin, X. F., Liu, D., Islam, E., Mahmood, Q., Yang, X. E., He, Z. L., Stoffella, P. J. (2009) Effects of zinc on root morphology and antioxidant adaptations of cadmium-treated Sedum alfredii H. J. Plant Nut. 32, 16421656.

    • Search Google Scholar
    • Export Citation
  • 19.

    Kheir, N. F., Harb, E. Z., Moursi, H. A., El-Gayar, S. H. (1991) Effect of salinity and fertilization on flax plants (Linum usitatissimum L.). II. Chemical composition. Bull. Fac. Agric. 42, 5770.

    • Search Google Scholar
    • Export Citation
  • 20.

    Laemmli, U. K. (1970) Cleavage of structural proteins during assembly of head bacteriophage T4. Nature 227, 680685.

  • 21.

    Lingua, G., Franchin, C., Todeschini, V., Castiglione, S., Biondi, S., Burlando, B., Parravicini, V., Torrigiani, P., Berta, G. (2008) Arbuscular mycorrhizal fungi differentially affect the response to high zinc concentrations of two registered poplar clones. Environ. Pollut. 153, 137147.

    • Search Google Scholar
    • Export Citation
  • 22.

    Manai, J., Kalail, T., Gouia, H., Corpas, F. J. (2014) Exogenous nitric oxide (NO) ameliorates salinity- induced oxidative stress in tomato (Solanum lycopersicum) plants. J. Soil Sci. and Plant Nut. 14, 433446.

    • Search Google Scholar
    • Export Citation
  • 23.

    Mohamed, H. I., Latif, H. H., Hanafy, R. S. (2016) Influence of nitric oxide application on some biochemical aspects, endogenous hormones, minerals and phenolic compounds of Vicia faba plant grown under arsenic stress. Gesunde Pflanzen 68, 99107.

    • Search Google Scholar
    • Export Citation
  • 24.

    Mostofa, M. G., Seraj, Z. I., Fujita, M. (2014) Exogenous sodium nitroprusside and glutathione alleviate copper toxicity by reducing copper uptake and oxidative damage in rice (Oryza sativa L.) seedlings. Protoplasma 251, 13731386.

    • Search Google Scholar
    • Export Citation
  • 25.

    Mukherjee, S. P., Choudhuri, M. A. (1983) Implications of water stress induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Plant Physiol. 58, 166170.

    • Search Google Scholar
    • Export Citation
  • 26.

    Mukhopadhyay, M., Das, A., Subba, P., Bantawa, P., Sarkar, B., Ghosh, P. D., Mondal, T. K. (2013) Structural, physiological and biochemical profiling of tea plants (Camellia sinensis (L.) O. Kuntze) under zinc stress. Biol. Plant. 57, 474480.

    • Search Google Scholar
    • Export Citation
  • 27.

    Nakano, Y., Asada, K. (1981) Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplast. Plant Cell Physiol. 22, 867880.

    • Search Google Scholar
    • Export Citation
  • 28.

    Neill, S. J., Desikan, R., Hancock, J. T. (2003) Nitric oxide signalling in plants. New Phytologist 159, 1135.

  • 29.

    Ozdener, Y., Aydin, B. K. (2010) The effect of zinc on the growth and physiological and biochemical parameters in seedlings of Eruca sativa (L.) (Rocket). Acta Physiol. Plant. 32, 469476.

    • Search Google Scholar
    • Export Citation
  • 30.

    Sawan, Z. M., Hafez, S. A., Basyony, A. E. (2001) Effect of nitrogen and zinc fertilization and plant growth retardants on cottonseed, protein, oil yields, and oil properties. J.A.O.C.S. 78, 10871092.

    • Search Google Scholar
    • Export Citation
  • 31.

    Thavaprakash, N., Siva Kumar, S. D., Raja, K., Senthil Kumar, G. (2002) Effect of nitrogen and phosphorus levels and ratios on seed yield and nutrient uptake of sunflower hybrid Dsh-I. Helia 25, 5968.

    • Search Google Scholar
    • Export Citation
  • 32.

    Thavaprakash, N., Senthil kumar, G., Siva kumar, S. D., Raju, M. (2003) Photosynthetic attributes and seed yield of sunflower as influenced by different levels and ratios of nitrogen and phosphorous fertilizers. Acta Agrono. Hungarica 51, 149155.

    • Search Google Scholar
    • Export Citation
  • 33.

    Vernon, L. P., Seely, G. R. (1966) The Chlorophylls. Academic, New York.

  • 34.

    Wang, C., Zhang, S. H., Wang, P. F., Qian, J., Hou, J., Zhang, W. J., Lu, J. (2009) Excess Zn alters the nutrient uptake and induces the antioxidative responses in submerged plant Hydrilla verticillata (L.f.) Royle. Chemosphere 76, 938945.

    • Search Google Scholar
    • Export Citation
  • 35.

    Xiong, J., An, L., Lu, H., Yhu, C. (2009) Exogenous nitric oxide enhances cadmium tolerance of rice by increasing pectin and hemicellulose contents in root cell wall. Planta 230, 755765.

    • Search Google Scholar
    • Export Citation
  • 36.

    Xu, L., Dong, Y., Fan, Z., Kong, J., Liu, S., Bai, X. (2014) Effects of the application of exogenous NO at different growth stage on the physiological characteristics of peanut grown in Cd-contaminated soil. J. Plant Interact. 9, 285296.

    • Search Google Scholar
    • Export Citation
  • 37.

    Xu, J., Yin, H., Li, Y., Liu, X. (2010) Nitric oxide is associated with long-term zinc tolerance in Solanum nigrum. Plant Physiol. 154, 13191334.

    • Search Google Scholar
    • Export Citation
  • 38.

    Younis, M. E., Gaber, A. M., El-Nimr, M. (2001) Plant growth, metabolism and adaptation of Glycine max and Phaseolus vulgaris subjected to anaerobic conditions and drought. Egyptian J. Physiol. Sci. 23, 273296.

    • Search Google Scholar
    • Export Citation

Editorial Board

      Csányi, Vilmos (Göd)
      Dudits, Dénes (Szeged)
      Falus, András (Budapest)
      Fischer, Ernő (Pécs)
      Gábriel, Róbert (Pécs)
      Gulya, Károly (Szeged)
      Gulyás, Balázs (Stockholm)
      Hajós, Ferenc (Budapest)
      Hámori, József (Budapest)
      Heszky, László (Gödöllő)
      Hideg, Éva (Szeged)
      E. Ito (Sanuki)
      Janda, Tibor (Martonvásár)
      Kavanaugh, Michael P. (Missoula)
      Kása, Péter (Szeged)
      Klein, Éva (Stockholm)
      Kovács, János (Budapest)
      Brigitte Mauch-Mani (Neuchâtel)
      Nässel, Dick R. (Stockholm)
      Nemcsók, János (Szeged)
      Péczely, Péter (Gödöllő)
      Roberts, D. F. (Newcastle-upon-Tyne)
      Sakharov, Dimitri A. (Moscow)
      Singh, Meharvan (Fort Worth)
      Sipiczky, Mátyás (Debrecen)
      Szeberényi, József (Pécs)
      Székely, György (Debrecen)
      Tari, Irma (Szeged)
      Vágvölgyi, Csaba (Szeged),
      L. Zaborszky (Newark)

 

Acta Biologica Hungarica
P.O. Box 35
H-8237 Tihany, Hungary
Phone: (36 87) 448 244 ext. 103
Fax: (36 87) 448 006
E-mail: elekes@tres.blki.hu

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture
  • Biology and Environmental Sciences
  • Elsevier BIOBASE
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Acta Biologica Hungarica
Language English
Size  
Year of
Foundation
1950
Publication
Programme
changed title
Volumes
per Year
 
Issues
per Year
 
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)