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Ádám Simon University of Debrecen, H-4027 Debrecen, Böszörményi út 138, Hungary

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János Oláh University of Debrecen, H-4027 Debrecen, Böszörményi út 138, Hungary

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István Komlósi University of Debrecen, H-4027 Debrecen, Böszörményi út 138, Hungary

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András Jávor University of Debrecen, H-4027 Debrecen, Böszörményi út 138, Hungary

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József Németh University of Debrecen, H-4032 Debrecen, Nagyerdei körút 98, Hungary

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Zoltán Szilvássy University of Debrecen, H-4032 Debrecen, Nagyerdei körút 98, Hungary

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Dóra Reglődi University of Pécs Medical School, H-7624 Pécs, Szigeti út 12, Hungary

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Andrea Tamás University of Pécs Medical School, H-7624 Pécs, Szigeti út 12, Hungary

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Levente Czeglédi University of Debrecen, H-4027 Debrecen, Böszörményi út 138, Hungary

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The list of orexigenic and anorexigenic peptides, those are known to alter feed intake, is continuously growing. However, most of them are studied in mammalian species. We aimed to investigate plasma level and mRNA expression of the pituitary adenylate cyclase-activating polypeptide (PACAP), gene expression of its receptor (PAC1), furthermore the gene expression of galanin (GAL), neuromedin U (NMU), and its two receptors (NMUR1 and NMUR2) in the hypothalamus, proventriculus, and jejunum of hens exposed to 40% calorie restriction. Feed restriction resulted in a 88% increase in mRNA and a 27% increase in peptide level of PACAP in proventriculus measured with qPCR and RIA, respectively. Increases were found in the gene expression of PAC1 (49%) and NMUR1 (63%) in the hypothalamus. Higher expressions of peptide encoding genes (76% for PACAP, 41% for NMU, 301% for NMUR1 and 308% for GAL, P < 0.05) were recorded in the jejunum of hens exposed to restricted nutrition. The results indicate that PACAP level responds to calorie restriction in the proventriculus and jejunum, but not in the hypothalamus and plasma.

  • 1.

    Andersen, C. L., Jensen, J. L., Ørntoft, T. F. (2004) Normalization of real-time quantitative reverse transcription-PCR data: A model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 64, 52455250.

    • Search Google Scholar
    • Export Citation
  • 2.

    Ando, R., Bungo, T., Kawakami, S. I., Shimojo, M., Masuda, Y., Furuse, M. (2000) Intracerebroventricular injection of mammalian motilin, melanin-concentrating hormone or galanin does not stimulate food intake in neonatal chicks. Br. Poult. Sci. 41, 508511.

    • Search Google Scholar
    • Export Citation
  • 3.

    Borzsei, R., Mark, L., Tamas, A., Bagoly, T., Bay, C., Csanaky, K., Banki, E., Kiss, P., Vaczy, A., Horvath, G., Nemeth, J., Szauer, E., Helyes, Z., Reglodi, D. (2009) Presence of pituitary adenylate cyclase activating polypeptide-38 in human plasma and milk. Eur. J. Endocrinol. 160, 561565.

    • Search Google Scholar
    • Export Citation
  • 4.

    Boswell, T. (2005) Regulation of energy balance in birds by the neuroendocrine hypothalamus. The Journal of Poultry Science. 2, 161181.

    • Search Google Scholar
    • Export Citation
  • 5.

    Cai, G., Mo, C., Huang, L., Li, J., Wang, Y. (2015) Characterization of the Two CART Genes (CART1 and CART2) in Chickens (Gallus gallus). PLoS One. 10, e0127107.

    • Search Google Scholar
    • Export Citation
  • 6.

    Clench, M., Mathias, J. (1995) Motility responses to fasting in the gastrointestinal-tract of 3 avian species. Condor. 97, 10411047.

  • 7.

    Czegledi, L., Tamas, A., Borzsei, R., Bagoly, T., Kiss, P., Horvath, G., Brubel, R., Nemeth, J., Szalontai, B., Szabadfi, K. (2011) Presence of pituitary adenylate cyclase-activating polypeptide (PACAP) in the plasma and milk of ruminant animals. Gen. Comp. Endocrinol. 172, 115119.

    • Search Google Scholar
    • Export Citation
  • 8.

    DeGolier, T. F., Nordell, J. N., Pust, M. H., Duke, G. E. (1999) Effect of galanin on isolated strips of smooth muscle from the gastrointestinal tract of chickens. J. Exp. Zool. 283, 463468.

    • Search Google Scholar
    • Export Citation
  • 9.

    Ding, B., Lenzi, C., Pirone, A. (2014) Immunohistochemical study on glucagon-like peptide-1 (GLP-1) and pituitary adenylate cyclase activating peptide (PACAP) in the small intestine of muscovy duck during the prehatching and posthatching periods. J. Poult. Sci. 51, 3541.

    • Search Google Scholar
    • Export Citation
  • 10.

    Dogrukol-Ak, D., Tore, F., Tuncel, N. (2004) Passage of VIP/PACAP/secretin family across the blood-brain barrier: therapeutic effects. Curr. Pharm. Des. 10, 13251340.

    • Search Google Scholar
    • Export Citation
  • 11.

    Domin, J., Benito-Orfila, M. A., Nandha, K. A., Aitken, A., Bloom, S. R. (1992) The purification and sequence analysis of an avian neuromedin U. Regul. Pept. 41, 18.

    • Search Google Scholar
    • Export Citation
  • 12.

    Furuse, M., Matsumoto, M., Mori, R., Sugahara, K., Kano, K., Hasegawa, S. (1997) Influence of fasting and neuropeptide Y on the suppressive food intake induced by intracerebroventricular injection of glucagon-like peptide-1 in the neonatal chick. Brain Res. 764, 289292.

    • Search Google Scholar
    • Export Citation
  • 13.

    Hagen, C. J., Newmyer, B. A., Webster, R. I., Gilbert, E. R., Siegel, P. B., Tachibana, T., Cline, M. A. (2013) Stimulation of food intake after central galanin is associated with arcuate nucleus activation and does not differ between genetically selected low and high body weight lines of chickens. Neuropeptides 47, 281285.

    • Search Google Scholar
    • Export Citation
  • 14.

    Helyes, Z., Pozsgai, G., Börzsei, R., Németh, J., Bagoly, T., Márk, L., Pintér, E., Tóth, G., Elekes, K., Szolcsányi, J. (2007) Inhibitory effect of PACAP-38 on acute neurogenic and non-neurogenic inflammatory processes in the rat. Peptides 28, 18471855.

    • Search Google Scholar
    • Export Citation
  • 15.

    Hiramatsu, K., Yamasaki, A. (2009) Immunohistochemical study on the innervation of the chicken pancreas by pituitary adenylate cyclase-activating polypeptides (PACAPs)-containing nerves. J. Poult. Sci. 46, 234239.

    • Search Google Scholar
    • Export Citation
  • 16.

    Honda, K., Saneyasu, T., Okuda, M., Uemura, T., Kamisoyama, H. (2015) Glucagon and neuromedin U suppress food intake in broiler chicks. J. Poult. Sci. 53, 268273.

    • Search Google Scholar
    • Export Citation
  • 17.

    Howard, A. D., Wang, R. P., Pong, S. S. (2000) Identification of receptors for neuromedin U and its role in feeding. Nature 406, 7074.

    • Search Google Scholar
    • Export Citation
  • 18.

    Jakab, B., Reglődi, D., Józsa, R., Hollósy, T., Tamás, A., Lubics, A., Lengvári, I., Oroszi, G., Szilvássy, Z., Szolcsányi, J., Németh, J. (2004) Distribution of PACAP-38 in the central nervous system of various species determined by a novel radioimmunoassay. J. Biochem. Biophys. Methods 61, 189198.

    • Search Google Scholar
    • Export Citation
  • 19.

    Jozsa, R., Nemeth, J., Tamas, A., Hollosy, T., Lubics, A., Peter, S., Olah, A., Lengvari, I. Reglodi, D. (2005) Short-term fasting differentially affects PACAP and VIP levels in rats and chicken. Regul. Pept. 130, 166.

    • Search Google Scholar
    • Export Citation
  • 20.

    Jozsa, R., Nemeth, J., Tamas, A., Hollosy, T., Lubics, A., Jakab, B., Olah, A., Lengvari, I., Arimura, A., Reglödi, D. (2006) Short-term fasting differentially alters PACAP and VIP levels in the brains of rat and chicken. Ann. N. Y. Acad. Sci. 1070, 354358.

    • Search Google Scholar
    • Export Citation
  • 21.

    Juhasz, T., Helgadottir, S. L., Tamas, A., Reglodi, D., Zakany, R. (2015) PACAP and VIP signaling in chondrogenesis and osteogenesis. Peptides 66, 5157.

    • Search Google Scholar
    • Export Citation
  • 22.

    Kamisoyama, H., Honda, K., Saneyasu, T., Sugahara, K., Hasegawa, S. (2007) Central administration of neuromedin U suppresses food intake in chicks. Neurosci. Lett. 420, 15.

    • Search Google Scholar
    • Export Citation
  • 23.

    Kiss, P., Reglődi, D., Tamás, A., Lubics, A., Lengvári, I., Józsa, R., Somogyvári-Vigh, A., Szilvássy, Z., Németh, J. (2007) Changes of PACAP levels in the brain show gender differences following shortterm water and food deprivation. Gen. Comp. Endocrinol. 152, 225230.

    • Search Google Scholar
    • Export Citation
  • 24.

    Klein, S., Jurkevich, A., Grossmann, R. (2006) Sexually dimorphic immunoreactivity of galanin and colocalization with arginine vasotocin in the chicken brain (Gallus gallus domesticus). J. Comp. Neurol. 499, 828839.

    • Search Google Scholar
    • Export Citation
  • 25.

    Kuenzel, W. J., Masson, M. (1988) A stereotaxic atlas of the brain of the chick (Gallus domesticus), The John Hopkins University Press, Baltimore, MD.

    • Search Google Scholar
    • Export Citation
  • 26.

    Kumano, S., Matsumoto, H., Takatsu, Y., Noguchi, J., Kitada, C., Ohtaki, T. (2003) Changes in hypothalamic expression levels of galanin-like peptide in rat and mouse models support that it is a leptintarget peptide. Endocrinology 144, 26342643.

    • Search Google Scholar
    • Export Citation
  • 27.

    Lam, S., Liu, Y., Liong, E., Tipoe, G., Fung, M. L. (2012) Upregulation of pituitary adenylate cyclase activating polypeptide and its receptor expression in the rat carotid body in chronic and intermittent hypoxia. Adv. Exp. Med. Biol. 758, 301306.

    • Search Google Scholar
    • Export Citation
  • 28.

    Luts, A., Uddman, R., Sundler, F. (1989) Neuronal galanin is widely distributed in the chicken respiratory tract and coexists with multiple neuropeptides. Cell Tissue Res. 256, 95103.

    • Search Google Scholar
    • Export Citation
  • 29.

    Matsuda, K., Maruyama, K., Nakamachi, T., Miura, T., Shioda, S. (2006) Effects of pituitary adenylate cyclase-activating polypeptide and vasoactive intestinal polypeptide on food intake and locomotor activity in the goldfish, Carassius auratus. Ann. N. Y. Acad. Sci. 1070, 417421.

    • Search Google Scholar
    • Export Citation
  • 30.

    Monir, M., Hiramatsu, K., Yamasaki, A., Nishimura, K., Watanabe, T. (2014) The influence of restricted feeding on glucagon-like peptide-1 (GLP-1)-containing cells in the chicken small intestine. Anat. Histol. Embryol. 43, 153158.

    • Search Google Scholar
    • Export Citation
  • 31.

    Oclon, E., Pietras, M. (2011) Peripheral ghrelin inhibits feed intake through hypothalamo-pituitaryadrenal axis-dependent mechanism in chicken. J. Anim. Feed Sci. 20, 118130.

    • Search Google Scholar
    • Export Citation
  • 32.

    Okimura, K., Sakura, N., Kurosawa, K., Hashimoto, T. (1992) Contractile activity of porcine neuromedin U-25 and various neuromedin U-related peptide fragments on isolated chicken crop smooth muscle. Chem. Pharm. Bull. 40, 15001503.

    • Search Google Scholar
    • Export Citation
  • 33.

    Ozawa, M., Aono, M., Moriga, M. (1999) Central effects of pituitary adenylate cyclase activating polypeptide (PACAP) on gastric motility and emptying in rats. Dig. Dis. Sci. 44, 735743.

    • Search Google Scholar
    • Export Citation
  • 34.

    Peeters, K., Gerets, H. H., Princen, K., Vandesande, F. (1999) Molecular cloning and expression of a chicken pituitary adenylate cyclase-activating polypeptide receptor. Mol. Brain Res. 71, 244255.

    • Search Google Scholar
    • Export Citation
  • 35.

    Peeters, K., Gerets, H. H., Arckens, L., Vandesande, F. (2000) Distribution of pituitary adenylate cyclase-activating polypeptide and pituitary adenylate cyclase-activating polypeptide type I receptor mRNA in the chicken brain. J. Comp. Neurol. 423, 6682.

    • Search Google Scholar
    • Export Citation
  • 36.

    Pirone, A., Baoan, D., Piano, I., Della Santina, L., Baglini, A., Lenzi, C. (2011) Pituitary adenylate cyclase-activating peptide (PACAP) immunoreactivity distribution in the small intestine of the adult New Hampshire chicken. Acta Histochem. 113, 477483.

    • Search Google Scholar
    • Export Citation
  • 37.

    Racz, B., Horvath, G., Reglodi, D., Gasz, B., Kiss, P., Gallyas, F., Sumegi, B., Toth, G., Nemeth, A., Lubics, A. (2010) PACAP ameliorates oxidative stress in the chicken inner ear: an in vitro study. Regul. Pept. 160, 9198.

    • Search Google Scholar
    • Export Citation
  • 38.

    Salvi, E., Vaccaro, R., Renda, T. G. (1999) Ontogeny of galanin-immunoreactive elements in the intrinsic nervous system of the chicken gut. Anat. Rec. 254, 2838.

    • Search Google Scholar
    • Export Citation
  • 39.

    Tachibana, T., Saito, S., Tomonaga, S., Takagi, T., Saito, E., Boswell, T., Furuse, M. (2003) Intracerebroventricular injection of vasoactive intestinal peptide and pituitary adenylate cyclaseactivating polypeptide inhibits feeding in chicks. Neurosci. Lett. 339, 203206.

    • Search Google Scholar
    • Export Citation
  • 40.

    Tachibana, T., Mori, M., Khan, M. S. I., Ueda, H., Sugahara, K., Hiramatsu, K. (2008) Central administration of galanin stimulates feeding behavior in chicks. Comp. Biochem. Physiol., Part A Mol. Integr. Physiol. 151, 637640.

    • Search Google Scholar
    • Export Citation
  • 41.

    Takeuchi, S., Teshigawara, K., Takahashi, S. (2000) Widespread expression of Agouti-related protein (AGRP) in the chicken: a possible involvement of AGRP in regulating peripheral melanocortin systems in the chicken. Biochim. Biophys. Acta-Mol. Cell Res. 1496, 261269.

    • Search Google Scholar
    • Export Citation
  • 42.

    Webster, A. B. (1995) Immediate and subsequent effects of a short fast on the behavior of laying hens. Appl. Anim. Behav. Sci. 45, 255266.

    • Search Google Scholar
    • Export Citation
  • 43.

    Yamamoto, I., Nakao, N., Kaiya, H., Miyazato, M., Tsushima, N., Arai, T., Tanaka, M. (2011) Two chicken neuromedin U receptors: Characterization of primary structure, biological activity and tissue distribution. Gen. Comp. Endocrinol. 174, 116123.

    • Search Google Scholar
    • Export Citation
  • 44.

    Yasuhara, T., Mizuno, K., Somogyvari-Vigh, A., Komaki, G., Arimura, A. (1992) Isolation and primary structure of chicken PACAP. Regul. Pept. 37, 326.

    • Search Google Scholar
    • Export Citation
  • 45.

    Yoshida, M., Aizawa, H., Takahashi, N., Shigyo, M., Hara, N. (2000) Pituitary adenylate cyclase activating peptide mediates inhibitory nonadrenergic noncholinergic relaxation. Eur. J. Pharmacol. 395, 7783.

    • Search Google Scholar
    • Export Citation
  • 46.

    Zentel, H., Nohr, D., Muller, S., Yanaihara, N., Weihe, E. (1990) Differential occurrence and distribution of galanin in adrenal nerve-fibers and medullary cells in rodent and avian species. Neurosci. Lett. 120, 167170.

    • Search Google Scholar
    • Export Citation
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Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

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Acta Biologica Hungarica
Language English
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Foundation
1950
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Founder Magyar Tudományos Akadémia
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ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)