Aymen Mabrouk University of Monastir, Street Avicenna, Monastir 5019, Tunisia

Search for other papers by Aymen Mabrouk in
Current site
Google Scholar
Restricted access

Alteration of the antioxidant system may be related to lead (Pb) hepatotoxicity. This study was carried out to investigate the possible beneficial effect of thymoquinone (TQ), the major active ingredient of volatile oil of Nigella sativa seeds, against Pb-induced liver antioxidant defense system impairment. Adult male rats were randomized into four groups: control group received no treatment, Pb group was exposed to 2000 ppm of Pb acetate in drinking water, Pb-TQ group was cotreated with Pb plus TQ (5 mg/kg/day, per os) and TQ group receiving only TQ. All treatments were applied for five weeks. TQ alone did not induce any significant changes in the enzymatic and non-enzymatic antioxidant status. By contrast, Pb exposure significantly decreased not only reduced glutathione level, but also superoxide dismutase, glutathione peroxidase, catalase and glutathione reductase activities in the liver tissue. Interestingly, when coadministrated with Pb, TQ significantly improved the affected antioxidant parameters. In conclusion, our results indicate a protective effect of TQ against Pb-induced liver antioxidant capacity impairment and suggest that this component might be a clinically promising alternative in Pb hepatotoxicity.

  • 1.

    Abdel-Wahab, W. M. (2013) Protective effect of thymoquinone on sodium fluoride-induced hepatotoxicity and oxidative stress in rats. J. Basic Appl. Zool. 66, 263270.

    • Search Google Scholar
    • Export Citation
  • 2.

    Ahmad, S., Beg, Z. H. (2013) Alleviation of plasma, erythrocyte and liver lipidemic-oxidative stress by thymoquinone and limonene in atherogenic suspension fed rats. J. Funct. Foods 5, 251259.

    • Search Google Scholar
    • Export Citation
  • 3.

    Arthur, J. R., Boyne, R. (1985) Superoxide dismutase and glutathione peroxidase activities in neutrophils from selenium deficient and copper deficient cattle. Life Sci. 36, 15691575.

    • Search Google Scholar
    • Export Citation
  • 4.

    Azoz, H. A., Raafat, R. M. (2012) Effect of lead toxicity on cytogenisity, biochemical constituents and tissue residue with protective role of activated charcoal and casein in male rats. Aust. J. Basic Appl. Sci. 6, 497509.

    • Search Google Scholar
    • Export Citation
  • 5.

    Çaylak, E., Halifeoğlu, İ., Aydin, S., Telo, S., Bulmuş, Ö., Çelik, H. (2007) The effects of sulfurcontaining compounds on total antioxidant capacity levels of liver, kidney and brain in lead-exposed rats. Turkiye Klinikleri J. Med. Sci. 27, 823828.

    • Search Google Scholar
    • Export Citation
  • 6.

    Cohen, G., Kim, M., Ogwu, V. (1996) A modified catalase assay suitable for a plate reader and for the analysis of brain cell cultures. J. Neurosci. Methods 67, 5356.

    • Search Google Scholar
    • Export Citation
  • 7.

    Darakhshan, S., Bidmeshki Pour, A., Hosseinzadeh Colagar, A., Sisakhtnezhad, S. (2015) Thymoquinone and its therapeutic potentials. Pharmacol. Res. 95–96, 138158.

    • Search Google Scholar
    • Export Citation
  • 8.

    Dewanjee, S., Sahu, R., Karmakar, S., Gangopadhyay, M. (2013) Toxic effects of lead exposure in Wistar rats: involvement of oxidative stress and the beneficial role of edible jute (Corchorus olitorius) leaves. Food Chem. Toxicol. 55, 7891.

    • Search Google Scholar
    • Export Citation
  • 9.

    Ellman, G. L. (1959) Tissue sulfhydryl groups. Arch. Biochem. Biophys. 82, 7077.

  • 10.

    El-Sayed, W. M. (2011) Upregulation of chemoprotective enzymes and glutathione by Nigella sativa (black seed) and thymoquinone in CCl4-intoxicated rats. Int. J. Toxicol. 30, 707714.

    • Search Google Scholar
    • Export Citation
  • 11.

    El-Sheikh, A. A., Morsy, M. A., Abdalla, A. M., Hamouda, A. H., Alhaider, I. A. (2015) Mechanisms of thymoquinone hepatorenal protection in methotrexate-induced toxicity in rats. Mediators Inflamm. 2015;2015:859383. doi: 10.1155/2015/859383. Epub 2015 May 21.

    • Search Google Scholar
    • Export Citation
  • 12.

    Farag, M. M., Ahmed, G. O., Shehata, R. R., Kazem, A. H. (2015) Thymoquinone improves the kidney and liver changes induced by chronic cyclosporine A treatment and acute renal ischaemia/reperfusion in rats. J. Pharm. Pharmacol. 67, 731739.

    • Search Google Scholar
    • Export Citation
  • 13.

    Goldberg, D. M., Spooner, R. J. (1983) Assay of glutathione reductase. In: Bergmeyen, H. V. (ed.) Methods of enzymatic analysis. Verlag Chemie, Deerfield Beach, pp. 258265.

    • Search Google Scholar
    • Export Citation
  • 14.

    Ismail, M., Al-Naqeep, G., Chan, K. W. (2010) Nigella sativa thymoquinone-rich fraction greatly improves plasma antioxidant capacity and expression of antioxidant genes in hypercholesterolemic rats. Free Radic. Biol. Med. 48, 664672.

    • Search Google Scholar
    • Export Citation
  • 15.

    Lakshmi, B. V., Sudhakar, M., Aparna, M. (2013) Protective potential of black grapes against lead induced oxidative stress in rats. Environ. Toxicol. Pharmacol. 35, 361368.

    • Search Google Scholar
    • Export Citation
  • 16.

    Liu, C. M., Zheng, Y. L., Lu, J., Zhang, Z. F., Fan, S. H., Wu, D. M., Ma, J. Q. (2010) Quercetin protects rat liver against lead-induced oxidative stress and apoptosis. Environ. Toxicol. Pharmacol. 29, 158166.

    • Search Google Scholar
    • Export Citation
  • 17.

    Matović, V., Buha, A., Ðukić-Ćosić, D., Bulat, Z. (2015) Insight into the oxidative stress induced by lead and/or cadmium in blood, liver and kidneys. Food Chem. Toxicol. 78, 130140.

    • Search Google Scholar
    • Export Citation
  • 18.

    Mudipalli, A. (2007) Lead hepatotoxicity and potential health effects. Indian J. Med. Res. 126, 518527.

  • 19.

    Newairy, A. S., Abdou, H. M. (2009) Protective role of flax lignans against lead acetate induced oxidative damage and hyperlipidemia in rats. Food Chem. Toxicol. 47, 813818.

    • Search Google Scholar
    • Export Citation
  • 20.

    Paglia, D. E., Valentine, W. N. (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med. 70, 158169.

    • Search Google Scholar
    • Export Citation
  • 21.

    Sayed-Ahmed, M. M., Aleisa, A. M., Al-Rejaie, S. S., Al-Yahya, A. A., Al-Shabanah, O. A., Hafez, M. M., Nagi, M. N. (2010) Thymoquinone attenuates diethylnitrosamine induction of hepatic carcinogenesis through antioxidant signaling. Oxid. Med. Cell. Longev. 3, 254261.

    • Search Google Scholar
    • Export Citation
  • 22.

    Sivaprasad, R., Nagaraj, M., Varalakshmi, P. (2004) Combined efficacies of lipoic acid and 2,3-dimercaptosuccinic acid against lead-induced lipid peroxidation in rat liver. J. Nutr. Biochem. 15, 1823.

    • Search Google Scholar
    • Export Citation
  • 23.

    Wang, Z., Yan, Y., Yu, X., Li, W., Li, B., Qin, C. (2016) Protective effects of chitosan and its watersoluble derivatives against lead-induced oxidative stress in mice. Int. J. Biol. Macromol. 83, 442449.

    • Search Google Scholar
    • Export Citation
  • Collapse
  • Expand

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)


Acta Biologica Hungarica
P.O. Box 35
H-8237 Tihany, Hungary
Phone: (36 87) 448 244 ext. 103
Fax: (36 87) 448 006

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture
  • Biology and Environmental Sciences
  • Elsevier BIOBASE
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • The ISI Alerting Services
  • Zoological Abstracts


Acta Biologica Hungarica
Language English
Year of
changed title
per Year
per Year
Founder Magyar Tudományos Akadémia
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)