View More View Less
  • 1 Alanya Alaaddin Keykubat University, Alanya, Antalya, Turkey
Restricted access

Ascorbic acid is an important antioxidant that plays role both on growth and development and also stress response of the plant. The purpose of this study was to determine the effect of ascorbate on physiological and biochemical changes of sunflower that was exposed to multiple stresses. Chlorophyll and carotenoid contents decreased and glutathione, ascorbate and malondialdehyde contents as well as antioxidant enzyme activities increased for sunflower plant that was exposed to 50 mM NaCl and pendimethalin at different concentrations. These changes were found to be more significant in groups simultaneously exposed to both stress factors. While malondialdehyde content decreased, chlorophyll, carotenoid, ascorbate, glutathione contents and antioxidant enzyme activities increased in plants treated exogenously with ascorbate, compared to the untreated samples. According to the findings of our study; compared to individual stress, the effect of stress is more pronounced in sunflower exposed to multiple stresses, and treatment with exogenous ascorbate reduces the negative effects of stress.

  • 1.

    Abbasi, M., Fakhani, E. (2015) Role of salicylic acid and ascorbic acid in the alleviation of salinity stres in wheat (Triticum aestivum L.) JBES 6, 107113.

    • Search Google Scholar
    • Export Citation
  • 2.

    Akerboom, T. P. M., Sies, H. (1981) Assay of glutathione, glutathione disulfide and glutathione mixed disulfide in biological samples, In: Jakoby, W. B. (ed.), Methods in Enzymology 77, Academic Press, New York, pp. 373382.

    • Search Google Scholar
    • Export Citation
  • 3.

    Akram, N. A., Ashraf, M., Al-Qurainy, F. (2012) Aminolevulinic acid-induced changes in some key physiological attributes and activities of antioxidant enzymes in sunflower (Helianthus annuus L.) plants under saline regimes. Sci. Hortic. 142, 143148.

    • Search Google Scholar
    • Export Citation
  • 4.

    Andrews, C. J. (2005) Purifcation and characterisation of a family of glutathione transferases with roles in herbicide detoxifcation in soybean (Glycine max L.); selective enhancement by herbicides and herbicide safeners. Pestic. Biochem. Phys. 82, 205219.

    • Search Google Scholar
    • Export Citation
  • 5.

    Anjum, N. A., Gill, S. S., Gill, R., Hasanuzzaman, M., Duarte, A. C., Pereira, E., Ahmad, I., Tuteja, R., Tuteja, N. (2014) Metal/metalloid stress tolerance in plants: role of ascorbate, its redox couple, and associated enzymes. Protoplasma 251, 12651283.

    • Search Google Scholar
    • Export Citation
  • 6.

    Appleby, A., Valverde, B. (1989) Behavior of dinitroaniline herbicides in plants. Weed Technol. 3, 198206.

  • 7.

    Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248254.

    • Search Google Scholar
    • Export Citation
  • 8.

    Bybordi, A. (2012) Effect of ascorbic acid and silicium on photosynthesis, antioxidant enzyme activity, and fatty acid contents in canola exposure to salt stress. JIA 11, 16101620.

    • Search Google Scholar
    • Export Citation
  • 9.

    Carlberg, I., Mannervik, B. (1985) Glutathione reductase. Method. Enzymol. 113, 484490.

  • 10.

    Çulha, Ş., Çakırlar, H. (2011) The effect of salinity on plants and salt tolerance mechanisms. AKU-J. Sci. Eng. 11, 1134.

  • 11.

    De-Kok, L., Graham, M. (1980) Levels of pigments, soluble proteins, amino acids and sulfhydryl compounds in foliar tissue of Arabidopsis thaliana during dark induced and natural senescence. Plant Physiol. Biochem. 27, 133142.

    • Search Google Scholar
    • Export Citation
  • 12.

    Duncan, D. B. (1955) Multiple range and multiple F tests biometrics. IBS 11, 142.

  • 13.

    Ebrahimian, E., Bybordi, A. (2011) Influence of different proportion of nitrate, ammonium and silicium on activity of antioxidant enzymes and some physiological traits in sunflower under conditions of salt stress. JFAE 9, 10521058.

    • Search Google Scholar
    • Export Citation
  • 14.

    Elloumi, N., Zouari, M., Chaari, L., Abdallah, F. B., Woodward, S., Kallel, M. (2015) Effect of phosphogypsum on growth, physiology, and the antioxidative defense system in sunflower seedlings. Environ. Sci. Pollut. 22, 1482914840.

    • Search Google Scholar
    • Export Citation
  • 15.

    Gill, S. S., Anjum, N. A., Hasanuzzaman, M., Gill, R., Trivedi, D. K., Ahmad, I., Pereira, E., Tuteja, N. (2013) Glutathione and glutathione reductase: A boon in disguise for plant abiotic stress defense operations. Plant Physiol. Biochem. 70, 204212.

    • Search Google Scholar
    • Export Citation
  • 16.

    Gill, S. S., Tuteja, N. (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol. Biochem. 48, 909930.

    • Search Google Scholar
    • Export Citation
  • 17.

    Habig, W. H., Pabst, M. J., Jakoby, W. B. (1974) The first enzymatic step in mercapturic acid formation Glutathion S-Transferases. J. Biol. Chem. 249, 71307139.

    • Search Google Scholar
    • Export Citation
  • 18.

    Haferkamp, M. R. (1988) Environmental factors affecting plant productivity. Achieving efficient use of rangeland resources. In: White, R. S., Short, R. E. (ed.), Fourth Keog Research Symposium. Montana Agr. Exp. Sta. Bozeman. p. 132.

    • Search Google Scholar
    • Export Citation
  • 19.

    Heath, R. L., Packer, L. (1968) Photoperoxidation in isolated chloroplast, I. Kinetics stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 125, 180198.

    • Search Google Scholar
    • Export Citation
  • 20.

    Jabeen, N., Ahmad, R. (2013) The activity of antioxidant enzymes in response to salt stress in safflower (Carthamus tinctorius L.) and sunflower (Helianthus annuus L.) seedlings raised from seed treated with chitosan. J. Sci. Food Agr. 93, 16991705.

    • Search Google Scholar
    • Export Citation
  • 21.

    Kalefetoğlu, T., Ekmekçi, Y. (2005) The effects of drought on plants and tolerance mechanisms. GUJS 18, 723740.

  • 22.

    Kaya, A., Doganlar, Z. B. (2016) Exogenous jasmonic acid induces stress tolerance in tobacco (Nicotiana tabaccum) exposed to imazapic. Ecotox. Environ. Safe 124, 470479.

    • Search Google Scholar
    • Export Citation
  • 23.

    Kaya, A., Yiğit, E. (2014) The physiological and biochemical effects of salicylic acid on sunflowers (Helianthus annuus) exposed to flurochloridone. Ecotox. Environ. Safe 106, 232238.

    • Search Google Scholar
    • Export Citation
  • 24.

    Khan, A., Lang, I., Amjid, M., Shah, A., Ahmad, I., Nawaz, H. (2013) Inducing salt tolerance on growth and yield of sunflower by appliying different levels of ascorbic acid. J. Plant Nutr. 36, 11801190.

    • Search Google Scholar
    • Export Citation
  • 25.

    Kostopoulou, Z., Therios, I., Roumeliotis, E., Kanellis, A. K., Molassiotis, A. (2015) Melatonin combined with ascorbic acid provides salt adaptation in Citrus aurantium L. seedlings. Plant Physiol. Biochem. 86, 155165.

    • Search Google Scholar
    • Export Citation
  • 26.

    Li, G., Wan, S., Zhou, J., Yang, Z., Qin, P. (2010) Leaf chlorophyll fluorescence, hyperspectral reflectance, pigments content, malondialdehyde and proline accumulation responses of castor bean (Ricinus communis L.) seedlings to salt stress levels. Ind. Crop. Prod. 31, 1319.

    • Search Google Scholar
    • Export Citation
  • 27.

    Lichtenthaler, K., Welburn, A. R. (1983) Determination of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Society Transactions, 603rd Meeting, Liverpool, pp. 591592.

    • Search Google Scholar
    • Export Citation
  • 28.

    Liu, J., Wang, W., Wang, L., Sun, Y. (2015) Exogenous melatonin improves seedling health index and drought tolerance in tomato. Plant Growth Regul. 77, 317326.

    • Search Google Scholar
    • Export Citation
  • 29.

    Mafakheri, A., Siosemardeh, A., Bahramnejad, B., Struik, P. C., Sohrabi, Y. (2010) Effect of drought stress on yield, proline and chlorophyll contents in three chickpea cultivars. AJCS 4, 580585.

    • Search Google Scholar
    • Export Citation
  • 30.

    Mandal, S., Yadav, S., Yadav, S., Nema, R. K. (2009) Antioxidants: A review. J. Chem. Pharm. Res 1, 102104.

  • 31.

    Mitsou, K., Koulianou, A., Lambropoulou, D., Pappas, P., Albanis, T., Lekka, M. (2006) Growth rate effects, responses of antioxidant enzymes and metabolic fate of the herbicide propanil in the aquatic plant Lemna minor. Chemosphere 62, 275284.

    • Search Google Scholar
    • Export Citation
  • 32.

    M.-Kalantari, K. H., Oloumi, H. (2005) Study the effects of CdCl2 on lipid peroxidation and antioxidant compounds content in Brassica napus. Iranian J. Sci. Technol. Trans. A, 29, 201208.

    • Search Google Scholar
    • Export Citation
  • 33.

    Mukherjee, S. P., Choudhuri, M. A. (1983) Implication of water stress induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiol. Plantarum 58, 166170.

    • Search Google Scholar
    • Export Citation
  • 34.

    Nakano, Y., Asada, K. (1981) Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol. 22, 867880.

    • Search Google Scholar
    • Export Citation
  • 35.

    Potters, G., Gara, L. D., Asard, H., Horemans, N. (2002) Ascorbate and glutathione: guardians of the cell cycle, partners in crime? Plant Physiol. Biochem. 40, 537548.

    • Search Google Scholar
    • Export Citation
  • 36.

    Sairam, R. K., Rao, K. V., Srivastava, G. C. (2002) Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Sci. 163, 10371046.

    • Search Google Scholar
    • Export Citation
  • 37.

    Santos, C. V. (2004) Regulation of chlorophyll biosynthesis and degradation by salt stress in sunflower leaves. Sci. Hortic. 103, 9399.

    • Search Google Scholar
    • Export Citation
  • 38.

    Sivaci, A., Kaya, A., Duman, S. (2014) Effects of ascorbic acid on some physiological changes of pepino (Solanum muriactum ait) under chilling stress. Acta Biol. Hung. 65, 305318.

    • Search Google Scholar
    • Export Citation
  • 39.

    Shigeoka, S., Ishikawa, T., Tamoi, M., Miyagawa, Y., Takeda, T., Yabuta, Y., Yoshimura, K. (2002) Regulation and function of ascorbate peroxidase isoenzymes. J. Exp. Bot. 53, 13051319.

    • Search Google Scholar
    • Export Citation
  • 40.

    Smirnoff, N. (1996) The function of metabolism of ascorbic acid in plants. Ann. Bot. London 78, 661669.

  • 41.

    Sondhia, S. (2012) Dissipation of pendimethalin in soil and its residues in chickpea (Cicer arietinum l.) under field conditions. B. Environ. Contam. Tox. 89, 10321036.

    • Search Google Scholar
    • Export Citation
  • 42.

    Wang W. , Vinocur, B., Altman, A. (2003) Plant responses to drought, salinity and extreme temperatures towards genetic engineering for stress tolerance. Planta 218, 114.

    • Search Google Scholar
    • Export Citation
  • 43.

    Verma, K., Shekhawat, G. S., Sharma, A., Mehta, S. K., Sharma, V. (2008) Cadmium induced oxidative stress and changes in soluble and ionically bound cell wall peroxidase activities in roots of seedling and 3–4 leaf stage plants of Brassica juncea (L.) czern. Plant Cell Rep. 27, 12611269.

    • Search Google Scholar
    • Export Citation
  • 44.

    Zhang, J., Kirkham, M. B. (1996) Antioxidant responses to drought in sunflower and sorghum seedlings. New Phytol. 132, 361373.