View More View Less
  • 1 Sohag University, 82524 Sohag, Egypt
Restricted access

Brassinosteroids (BRs) are considered to possess protective activity in plants exposed to various stresses. The present study was conducted to evaluate the effects of 24-epibrassinolide (EBL) on salt stressed summer squash cv. Eskandrani seedlings, whether it can alleviate the deleterious effects of salt stress in growing seedlings or not. For this, summer squash seeds were germinated in solidified half strength MS (Murashige and Skoog) medium supplemented with different concentrations and combinations of EBL (0, 5, 10 and 20 μM) and NaCl (0, 50, 100 and 150 mM). The different concentrations (5, 10, 20 μM) of EBL significantly increased germination percentage and seedling growth capacity and the greatest increase was observed at 10 μM EBL. EBL application significantly increased the contents of photosynthetic pigments, the relative water content and the uptake of K and Ca. However, the different concentrations (50, 100 and 150 mM) of NaCl significantly decreased the above-mentioned attributes. The different concentrations (50, 100 and 150 mM) of NaCl significantly increased the electrolyte leakage, the lipid peroxidation and the Na uptake, but the interaction between EBL and NaCl significantly decreased these parameters. The results of this study proved that the application of 24-epibrassinolide to growing squash seedlings under salt stress conditions reduced the deleterious effects of salt stress and increased the tolerance of seedlings to its detrimental effects.

  • 1.

    Adem, G. D., Roy, S. J., Zhou, M., Bowman, J. P., Shabala, S. (2014) Evaluating contribution of ionic, osmotic and oxidative stress components towards salinity tolerance in barley. BMC Plant Biol. 14, 113.

    • Search Google Scholar
    • Export Citation
  • 2.

    Agami, R. A. (2013) Alleviating the adverse effects of NaCl stress in maize seedlings by pretreating seeds with salicylic acid and 24-epibrassinolide. South African J. Bot. 88, 171177.

    • Search Google Scholar
    • Export Citation
  • 3.

    Ali, A. A., Abdel-Fattah, R. I. (2006) Osmolytes-antioxidant behaviour in Phaseolus vulgaris and Hordeum vulgare with brassinosteroid under salt stress. J. Agron. 5, 167174.

    • Search Google Scholar
    • Export Citation
  • 4.

    Anuradha, S., Rao, S. S. R. (2003) Effect of brassinosteroids on salinity stress induced inhibition of seed germination and seedling growth of rice (Oryza sativa L.). Plant Growth Regul. 33, 151153.

    • Search Google Scholar
    • Export Citation
  • 5.

    Arnon, D. I. (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol. 24, 115.

  • 6.

    Arora, N., Bhardwaj, R., Sharma, P., Arora, H. K. (2008) 28-Homobrassinolide alleviates oxidative stress in salt treated maize (Zea mays L.) plants. Braz J. Plant Physiol. 20, 153157.

    • Search Google Scholar
    • Export Citation
  • 7.

    Asghari, M., Zahedipour, P. (2016) 24-Epibrassinolide acts as a growth-promoting and resistancemediating factor in strawberry plants. Plant Growth Regul. 35, 722729.

    • Search Google Scholar
    • Export Citation
  • 8.

    Azooz, M. M. (2009) Salt stress mitigation by seed priming with salicylic acid in two faba bean genotypes differing in salt tolerant. Int. J. Agric. Biol. 11, 343350.

    • Search Google Scholar
    • Export Citation
  • 9.

    Bajguz, A. (2000) Effect of brassinosteroids on nucleic acids and protein content in cultured cells of Chlorella vulgaris. Plant Physiol. Biochem. 38, 209215.

    • Search Google Scholar
    • Export Citation
  • 10.

    Bates, L. S., Waldren, R. P., Teare, I. D. (1973) Rapid determination of free proline for water stress studies. Plant and Soil 29, 205207.

    • Search Google Scholar
    • Export Citation
  • 11.

    Behnamnia, M., Kalantari, K. H. M., Rezanejad, F. (2009) Exogenous application of brassinosteroid alleviates drought-induced oxidative stress in Lycopersicon esculentum L. General Appl. Plant Physiol. 35, 2234.

    • Search Google Scholar
    • Export Citation
  • 12.

    Bjornson, M., Dandekar, A. M., Chory, J., Dehesh, K. (2016) Brassinosteroid’s multi-modular interaction with the general stress network customizes stimulus-specific responses in Arabidopsis. Plant Sci. 250, 165177.

    • Search Google Scholar
    • Export Citation
  • 13.

    Clouse, S. D., Sasse, J. M. (1998) Brassinosteroids: essential regulators of plant growth and development. Annu. Rev. Plant Physiol. Plant. Mol. Biol. 49, 427451.

    • Search Google Scholar
    • Export Citation
  • 14.

    Çoban, Ö. , Baydar, N. G. (2016) Brassinosteroid effects on some physical and biochemical properties and secondary metabolite accumulation in peppermint (Mentha piperita L.) under salt stress. Ind. Crops Prod. 86, 251258.

    • Search Google Scholar
    • Export Citation
  • 15.

    Dubios, M., Gilles, K. A., Hamilton, J. K., Reberes, P. A., Smith, F. (1956) Colometric method for determination of sugar and related substances. Anal. Chem. 28, 350356.

    • Search Google Scholar
    • Export Citation
  • 16.

    Fariduddin, Q., Yusuf, M., Hayat, S., Ahmad, A. (2009) Effect of 28-homobrassinolide on antioxidant capacity and photosynthesis in Brassica juncea plants exposed to different levels of copper. Env. Exp. Bot. 66, 418424.

    • Search Google Scholar
    • Export Citation
  • 17.

    Galal, A. (2017) Physico-chemical changes in karkade (Hibiscus sabdariffa L.) seedlings responding to salt stress. Acta Biol. Hung. 68, 7387.

    • Search Google Scholar
    • Export Citation
  • 18.

    Hayat, S., Hasan, S., Am, Yusuf, M., Hayat, Q., Ahmad, A. (2010) Effect of 28-homobrassinolide on photosynthesis, fluorescence and antioxidant system in the presence or absence of salinity and temperature in Vigna radiata. Env. Exp. Bot. 69, 105112.

    • Search Google Scholar
    • Export Citation
  • 19.

    Heath, R. L., Packer, L. (1969) Photoperoxidation in isolated chloroplast. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch. Biochem. Biophys. 125, 189198.

    • Search Google Scholar
    • Export Citation
  • 20.

    Hepler, P. K., Wayne, R. O. (1985) Calcium and plant development. Annual Rev. Plant Physiol. 36, 391397.

  • 21.

    Houimli, S. I. M., Denden, M., Mouhandes, B. D. (2010) Effects of 24-epibrassinolide on growth, chlorophyll, electrolyte leakage and proline by pepper plants under NaCl-stress. EurAsian J. BioSci. 4, 96104.

    • Search Google Scholar
    • Export Citation
  • 22.

    Jain, M., Mathar, G., Koul, S., Sarin, N. B. (2001) Ameliorative effects of proline on salt stressinduced lipid peroxidation in cell lines of groundnut (Arachis hypogeal L.). Plant Cell Rep. 20, 463468.

    • Search Google Scholar
    • Export Citation
  • 23.

    Jiang, Q. O., Deng, X. Z., Zhan, J. Y., Yan, H. M. (2011) Impacts of economic development on ecosystem risk in the Yellow River Delta. Procedia Env. Sci. 5, 208218.

    • Search Google Scholar
    • Export Citation
  • 24.

    Kamel, M. (2007) Osmotic adjustment in three succulent species of Zygophyllaceae. Afr. J. Eco. 46, 96104.

  • 25.

    Katerji, N., Van Hoorn, J. W., Hamdy, A., Mastrorilli, M., Mou Karzel, E. (1997) Osmotic adjustment of sugar beets in response to soil salinity and its influence on stomatal conductance, growth and yield. Agric Water Manage 34, 5769.

    • Search Google Scholar
    • Export Citation
  • 26.

    Kavi Kishor, P. B., Sangam, S., Amruth, R. N., Sri Laxmi, P., Naidu, K. R., Rao, K. R. S. S., Sreenath Rao Reddy, K. J., Theriappan, P., Sreenivasulu, N. (2005) Regulation of proline biosynthesis, degradation, uptake and transport in higher plants: its implications in plant growth and abiotic stress tolerance. Cur. Sci. 88, 424438.

    • Search Google Scholar
    • Export Citation
  • 27.

    Kaya, C., Tuna, A. L., Ashraf, M., Altunlu, H. (2007) Improved salt tolerance of melon (Cucumis melo L.) by the addition of proline and potassium nitrate. Environ. Exp. Bot. 60, 397403.

    • Search Google Scholar
    • Export Citation
  • 28.

    Li, L., Xu, J., Xu, Z. H., Xue, H. W. (2005) Brassinosteroids stimulate plant tropisms through modulation of polar auxin transport in Brassica and Arabidopsis. Plant Cell 17, 27382753.

    • Search Google Scholar
    • Export Citation
  • 29.

    Lutts, S., Kinet, J. M., Bouharmont, J. (1996) NaCl induced senescence in leaves of rice (Oryza sativa L.) cultivars differing in salinity resistance. Ann Bot. 78, 389398.

    • Search Google Scholar
    • Export Citation
  • 30.

    Ma, H. Y., Song, L. R., Shu, Y. J. (2012) Comparative proteomic analysis of seedling leaves of different salt tolerant soybean genotypes. J. Proteomics 75, 15291546.

    • Search Google Scholar
    • Export Citation
  • 31.

    Mazliak, P. (1983) Plant membrane lipids: changes and alterations during aging and senescence. In: Lieberman, M. (ed.), Post-Harvest Physiology and Crop Preservation, Plenum Press, New York, pp. 123140.

    • Search Google Scholar
    • Export Citation
  • 32.

    Munns, R., James, R. A., Xu, B. (2012) Wheat grain yield on saline soils is improved by an ancestral Na+ transporter gene. Nature Biotechnol. 30, 360364.

    • Search Google Scholar
    • Export Citation
  • 33.

    Murashige, T., Skoog, F. (1962) A revised medium for a rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473497.

    • Search Google Scholar
    • Export Citation
  • 34.

    Mussig, C., Shine, G. H., Altman, T. (2003) Brassinosteroids promote root growth in Arabidopsis. Plant Physiol. 133, 12611271.

  • 35.

    Nakashita, H., Yasuda, M., Nitta, T., Asami, T., Fujikoa, S., Arai, Y., Sekimata, K., Takatsuto, S., Yamaguchi, I., Yoshida, S. (2003) Brassinosteroids functions in a broad range of disease resistance in tobacco and rice. Plant Journal, 33, 887898.

    • Search Google Scholar
    • Export Citation
  • 36.

    Ogweno, J. O., Song, X. S., Shi, K., Hu, W. H., Mao, W. H., Zhou, Y. H., Yu, J. Q., Nogues, S. (2008) Brassinosteroids alleviate heat-induced inhibition of photosynthesis by increasing carboxylation efficiency and enhancing antioxidant systems in Lycopersicon esculentum. Plant Growth Regul. 27, 4957.

    • Search Google Scholar
    • Export Citation
  • 37.

    Ozdemir, F., Bor, M., Demiral, T., Turkan, I. (2004) Effects of 24-epibrassinolide on seed germination, seedling growth, lipid peroxidation, proline content and anti-oxidative system of rice (Oriza sativa L.) under alinity stress. Plant Growth Regul. 42, 203211.

    • Search Google Scholar
    • Export Citation
  • 38.

    Rajewska, W., Talarek, M., Bajguz, A. (2016) Brassinosteroids and response of plants to heavy metals action. Front Plant Sci. 7, 629633.

    • Search Google Scholar
    • Export Citation
  • 39.

    Sairam, R. K., Rao, K. V., Srivastava, G. C. (2005) Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Sci. 163, 10371046.

    • Search Google Scholar
    • Export Citation
  • 40.

    Shahid, M. A., Pervez, M. A., Balal, R. M., Mattson, N. S., Rashid, A., Ahmad, R., Ayyub, C. M., Abba, T. (2011) Brassinosteroid (24-epibrassinolide) enhances growth and alleviates the deleterious effects induced by salt stress in pea (Pisum sativum L.). Aust. J. Crop Sci. 5, 500510.

    • Search Google Scholar
    • Export Citation
  • 41.

    Soares, C., deSousa, A., Pinto, A., Azenha, M., Teixeira, J., Azevedo, R. A. (2016) Effect of 24-epibrassinolide on ROS content, antioxidantsystem, lipid peroxidation and Ni uptake in Solanum nigrum L. under Ni stress. Environ. Exp. Bot. 122, 115125.

    • Search Google Scholar
    • Export Citation
  • 42.

    Vardhini, B. V., Rao, S. S. R. (2003) Amelioration of osmotic stress by brassinosteroids on seed germination and seedling growth of three varieties of sorghum. Plant Growth Regul. 41, 2531.

    • Search Google Scholar
    • Export Citation
  • 43.

    Voigt, E. L., Caitano, R. F., Maia, J. M., Ferreira-Silva, S. L., De Macêdo, C. E. C., Silveira, J. A. G. (2009) Involvement of cation channels and NH4+ sensitive K+ transporters in Na+ uptake by cowpea roots under salinity. Biol. Plant. 53, 764768.

    • Search Google Scholar
    • Export Citation
  • 44.

    Wang, S. M., Wan, C. G., Wang, Y. R., Chen, H., Zhou, Z. Y., Fu, H., Sosebee, R. E. (2004) The characteristics of Na+, K+ and free proline distribution in several drought-resistant plants of the Alxa Desert. China J. Arid Environ. 56, 525539.

    • Search Google Scholar
    • Export Citation
  • 45.

    Yamasaki, S., Dillenburg, L. R. (1999) Measurements of leaf relative water content in Araucaria angustifolia. Revista Brasilleira de Fisiologia Vegetal, 11, 6975.

    • Search Google Scholar
    • Export Citation
  • 46.

    Yildirima, E., Taylorb, A. G., Spittlerb, T. D. (2006) Ameliorative effects of biological treatments on growth of squash plants under salt stress. Scientia Horticul. 111, 16.

    • Search Google Scholar
    • Export Citation
  • 47.

    Yu, J. Q., Huang, L. F., Hu, W. H., Zhou, Y. H., Mao, W. H., Ye, S. F., Nogues, S. (2004) A role for brassinosteroids in the regulation of photosynthesis in Cucumis sativus. J. Exp. Bot. 55, 11351143.

    • Search Google Scholar
    • Export Citation
  • 48.

    Zhabinskii, V. N., Khripach, N. B., Khripach, V. A. (2015) Steroid plant hormones: effects outside plant kingdom. Steroids 97, 8797.

Editorial Board

      Csányi, Vilmos (Göd)
      Dudits, Dénes (Szeged)
      Falus, András (Budapest)
      Fischer, Ernő (Pécs)
      Gábriel, Róbert (Pécs)
      Gulya, Károly (Szeged)
      Gulyás, Balázs (Stockholm)
      Hajós, Ferenc (Budapest)
      Hámori, József (Budapest)
      Heszky, László (Gödöllő)
      Hideg, Éva (Szeged)
      E. Ito (Sanuki)
      Janda, Tibor (Martonvásár)
      Kavanaugh, Michael P. (Missoula)
      Kása, Péter (Szeged)
      Klein, Éva (Stockholm)
      Kovács, János (Budapest)
      Brigitte Mauch-Mani (Neuchâtel)
      Nässel, Dick R. (Stockholm)
      Nemcsók, János (Szeged)
      Péczely, Péter (Gödöllő)
      Roberts, D. F. (Newcastle-upon-Tyne)
      Sakharov, Dimitri A. (Moscow)
      Singh, Meharvan (Fort Worth)
      Sipiczky, Mátyás (Debrecen)
      Szeberényi, József (Pécs)
      Székely, György (Debrecen)
      Tari, Irma (Szeged)
      Vágvölgyi, Csaba (Szeged),
      L. Zaborszky (Newark)

 

Acta Biologica Hungarica
P.O. Box 35
H-8237 Tihany, Hungary
Phone: (36 87) 448 244 ext. 103
Fax: (36 87) 448 006
E-mail: elekes@tres.blki.hu

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture
  • Biology and Environmental Sciences
  • Elsevier BIOBASE
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Acta Biologica Hungarica
Language English
Size  
Year of
Foundation
1950
Publication
Programme
changed title
Volumes
per Year
 
Issues
per Year
 
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)