View More View Less
  • 1 Department of Physiology, Melaka Manipal Medical College (Manipal Campus), Manipal University, Manipal 576104, India
  • | 1 Department of Physiology, RAK College of Medical Sciences, RAK Medical & Health Sciences University, PO Box. 11172, Ras Al Khaimah, UAE
  • | 2 College of Science and Health Professions – Jeddah, King Saud Bin Abdulaziz University for Health Sciences, National Guard Health Affairs, P. O. Box 9515, Jeddah 21423, Kingdom of Saudi Arabia
Restricted access

In the behavioral science field, many of the oldest tests have still most frequently been used almost in the same way for decades. The subjective influence of human observer and the large inter-observer and interlab differences are substantial among these tests. This necessitates the possibility of using technological innovations for behavioral science to obtain new parameters, results and insights as well. The light-dark box (LDB) test is a characteristic tool used to assess anxiety in rodents. A complete behavioral analysis (including both anxiety and locomotion parameters) is not possible by performing traditional LDB test protocol, as it lacks the usage of a real-time video recording of the test. In the current report, we describe an improved approach to conduct LDB test using a real-time video tracking system.

  • 1.

    Aarts, E., Maroteaux, G., Loos, M., Koopmans, B., Kovačević, J., Smit, A. B., Verhage, M., Sluis, Sv. (2015) Neuro-BSIK Mouse Phenomics Consortium: The light spot test: Measuring anxiety in mice in an automated home-cage environment. Behav. Brain. Res. 294, 123130.

    • Search Google Scholar
    • Export Citation
  • 2.

    Antsiferova, A., Kopaeva, M., Kashkarov, P. (2018) Effects of prolonged silver nanoparticle exposure on the contextual cognition and behavior of mammals. Materials (Basel). 11. pii: E558.

    • Search Google Scholar
    • Export Citation
  • 3.

    Aulich, D. (1976) Escape versus exploratory activity: an interpretation of rats’ behaviour in the open field and a light-dark preference test. Behav. Processes. 1, 153164.

    • Search Google Scholar
    • Export Citation
  • 4.

    Bedse, G., Bluett, R. J., Patrick, T. A. (2018) Therapeutic endocannabinoid augmentation for mood and anxiety disorders: comparative profiling of FAAH, MAGL and dual inhibitors. Transl. Psychiatry. 8, 92.

    • Search Google Scholar
    • Export Citation
  • 5.

    Belzung, C., Pineau, N., Beuzen, A., Misslin, R. (1994) PD135158, a CCKB antagonist, reduces ‘‘state’’, but not ‘‘trait’’ anxiety in mice. Pharmacol. Biochem. Behav. 49, 433436.

    • Search Google Scholar
    • Export Citation
  • 6.

    Bourin, M., Hascoët, M. (2003) The mouse light/dark box test. Eur. J. Pharmacol. 463, 5565.

  • 7.

    Cancela, L. M., Bregonzio, C., Molina, V. A. (1995) Anxiolytic-like effect induced by chronic stress is reversed by naloxone pretreatment. Brain. Res. Bull. 36, 209213.

    • Search Google Scholar
    • Export Citation
  • 8.

    Chaouloff, F., Durand, M., Mormède, P. (1997) Anxiety- and activity-related effects of diazepam and chlordiazepoxide in the rat light/dark and dark/light tests. Behav. Brain. Res. 85, 2735.

    • Search Google Scholar
    • Export Citation
  • 9.

    Chen, Z., Wei, H1., Pertovaara, A., Wang, J., Carlson, S. (2018) Anxiety- and activity-related effects of paracetamol on healthy and neuropathic rats. Pharmacol Res Perspect. 6.

    • Search Google Scholar
    • Export Citation
  • 10.

    Costall, B., Domeney, A. M., Kelly, M. E., Tomkins, D. M., Naylor, R. J., Wong, E. H. F., Smith, W. L., Whiting, R. L., Eglen, R. (1993) The effect of the 5-HT3 receptor antagonist, RS 42358-197, in animal models of anxiety. Eur. J. Pharmacol. 234, 9199.

    • Search Google Scholar
    • Export Citation
  • 11.

    Costall, Craig, K. J., Brown, K. J., Baum, A. (1995) Environmental factors in the etiology of anxiety. In: Bloom, F. E., Kupfer, D. J. (eds) Psychopharmacology: The Fourth Generation of Progress. Raven Press; New York, NY, 13251339.

    • Search Google Scholar
    • Export Citation
  • 12.

    Craig, K. J., Brown, K. J., Baum, A. (1995) Environmental factors in the etiology of anxiety. In: Bloom, F. E., Kupfer, D. J. (eds) Psychopharmacology: the Fourth Generation of Progress. Raven Press; New York, NY: 13251339.

    • Search Google Scholar
    • Export Citation
  • 13.

    Crawley, J., Goodwin, F. K. (1980) Preliminary report of a simple animal behavior model for the anxiolytic effects of benzodiazepines. Pharmacol. Biochem. Behav. 13, 167170.

    • Search Google Scholar
    • Export Citation
  • 14.

    Crawley, J. N. (1981) Neuropharmacologic specificity of a simple animal model for the behavioral actions of benzodiazepines. Pharmacol. Biochem. Behav. 15, 695699.

    • Search Google Scholar
    • Export Citation
  • 15.

    Crawley, J. N. (1985) Exploratory behaviour models of anxiety in mice. Neurosci. Biobehav. Rev. 9, 3744.

  • 16.

    Crawley, J. N., Belknap, J. K., Collins, A., Crabbe, J. C., Frankel, W., Henderson, N., Hitzeman, R. J., Maxson, S. C., Miner, L. L., Silva, A. J., Wehner, J. M., Wynshaw-Boris, A., Paylor, R. (1997) Behavioural phenotypes of inbred mouse strains: implication and recommendations for molecular studies. Psychopharmacology 132, 107124.

    • Search Google Scholar
    • Export Citation
  • 17.

    de Almeida Magalhães, T., Correia, D., de Carvalho, L. M., Damasceno, S., Brunialti Godard, A. L. (2017) Maternal separation affects expression of stress response genes and increases vulnerability to ethanol consumption. Brain Behav. 8, e00841.

    • Search Google Scholar
    • Export Citation
  • 18.

    Denenberg, V. H. (1969) Open-field behavior in the rat: what does it mean? Ann. N. Y. Acad. Sci. 159, 852859.

  • 19.

    Diaz, M. R., Mooney, S. M., Varlinskaya, E. I. (2016) Acute prenatal exposure to ethanol on gestational day 12 elicits opposing deficits in social behaviors and anxiety-like behaviors in Sprague Dawley rats. Behav Brain ResSep. 1, 310, 1119.

    • Search Google Scholar
    • Export Citation
  • 20.

    Ennaceur, A., Chazot, P. L. (2016) Preclinical animal anxiety research –flaws and prejudices. Pharmacol. Res. Perspect. 4, e00223.

  • 21.

    Fajemiroye, J. O., Adam, K., Jordan, K. Z., Alves, C. E., Aderoju, A. A. (2018) Evaluation of Anxiolytic and Antidepressant-like Activity of Aqueous Leaf Extract of Nymphaea Lotus Linn. in Mice. Iran J Pharm Res. 17, 613626.

    • Search Google Scholar
    • Export Citation
  • 22.

    Fu, K., Miyamoto, Y., Sumi, K. (2017) Overexpression of transmembrane protein 168 in the mouse nucleus accumbens induces anxiety and sensorimotor gating deficit. PLoS One, 12, e0189006.

    • Search Google Scholar
    • Export Citation
  • 23.

    Gao, B., Cutler, M. G. (1992) Effect of acute administration of the 5-HT3 receptor antagonist, BRL 46470A, on the behavior of mice in a two compartment light-dark box and during social interactions in their home cage and an unfamiliar neutral cage. Neuropharmacology 31, 743748.

    • Search Google Scholar
    • Export Citation
  • 24.

    Garcia, A. M. B., Martinez, R. C. R., Morato, S. (2008) Preference for the light compartment of a light/dark cage does not affect rat exploratory behavior in the elevated plus-maze. Psychol. Neurosci. 1, 7380.

    • Search Google Scholar
    • Export Citation
  • 25.

    Hall, C., Ballachey, E. L. (1932) A study of the rat’s behavior in a field. A contribution to method in comparative psychology. University of California Publications in Psychology 6, 112.

    • Search Google Scholar
    • Export Citation
  • 26.

    Haller, J., Alicki, M. (2012) Current animal models of anxiety, anxiety disorders, and anxiolytic drugs. Curr. Opin. Psychiatry 25, 5964.

    • Search Google Scholar
    • Export Citation
  • 27.

    Haller, J., Aliczki, M., Gyimesine Pelczer, K. (2013) Classical and novel approaches to the preclinical testing of anxiolytics: a critical evaluation. Neurosci. Biobehav. Rev. 37, 23182330.

    • Search Google Scholar
    • Export Citation
  • 28.

    Hascoet, M., Bourin, M., Colombel, M. C., Fiocco, A. J., Baker, G. B. (2000) Anxiolytic like effects of antidepressants after acute administration in a four plate test in mice. Pharmacol. Biochem. Behav. 65, 339344.

    • Search Google Scholar
    • Export Citation
  • 29.

    Jin, S., Zhao, Y., Jiang, Y. (2018) Anxiety-like behaviour assessments of adolescent rats after repeated maternal separation during early life. Neuroreport 29, 643649.

    • Search Google Scholar
    • Export Citation
  • 30.

    Kliethermes, C. L. (2005) Anxiety-like behaviors following chronic ethanol exposure. Neurosci. Biobehav. Rev. 28, 837850.

  • 31.

    Kumar, R. S., Narayanan, S. N., Nayak, S. (2009) Ascorbic acid protects against restraint stressinduced memory deficits in Wistar rats. Clinics (Sao Paulo). 64, 12111217.

    • Search Google Scholar
    • Export Citation
  • 32.

    Laureano-Melo, R., da Silveira, A. L., de Azevedo Cruz Seara, F., da Conceição, R. R., da Silva-Almeida, C., Marinho, B. G., da Rocha, F. F., Reis, L. C., Côrtes W da, S. (2016) Behavioral profile assessment in offspring of Swiss mice treated during pregnancy and lactation with caffeine. Metab. Brain. Dis. 31, 10711080.

    • Search Google Scholar
    • Export Citation
  • 33.

    Lukkes, J. L., Watt, M. J., Lowry, C. A., Forster, G. L. (2009) Consequences of Post-Weaning Social Isolation on Anxiety Behavior and Related Neural Circuits in Rodents. Front. Behav. Neurosci. 3, 18.

    • Search Google Scholar
    • Export Citation
  • 34.

    McIlwain, K. L., Merriweather, M. Y., Yuva-Paylor, L. A., Paylor, R. (2001) The use of behavioral test batteries: effects of training history. Physiol. Behav. 73, 705717.

    • Search Google Scholar
    • Export Citation
  • 35.

    Montgomery, K. C. (1958) The relation between fear induced by novel stimulation and exploratory behavior. J. Comp. Physiol. Psychol. 48, 254260.

    • Search Google Scholar
    • Export Citation
  • 36.

    Narayanan, S. N., Kumar, R. S., Karun, K. M., Nayak, S. B., Bhat, P. G. (2015) Possible cause for altered spatial cognition of prepubescent rats exposed to chronic radiofrequency electromagnetic radiation. Metab Brain Dis. 30, 11931206.

    • Search Google Scholar
    • Export Citation
  • 37.

    Narayanan, S. N., Kumar, R. S., Paval, J., Kedage, V., Bhat, M. S., Nayak, S., Bhat, P. G. (2013) Analysis of emotionality and locomotion in radio-frequency electromagnetic radiation exposed rats. Neurol Sci. 34, 11171124. Epub 2012 Sep 14.

    • Search Google Scholar
    • Export Citation
  • 38.

    Narayanan, S. N., Kumar, R. S., Paval, J., Nayak, S. (2010) Effect of ascorbic acid on the monosodium glutamate-induced neurobehavioral changes in periadolescent rats. Bratisl. Lek. Listy 111, 247252.

    • Search Google Scholar
    • Export Citation
  • 39.

    Patro, G., Kumar Bhattamisra, S., Kumar Mohanty, B. (2016) Effects of Mimosa pudica L. leaves extract on anxiety, depression and memory. Avicenna J. Phytomed. 6, 696710.

    • Search Google Scholar
    • Export Citation
  • 40.

    Ramos, A., Pereira, E., Martins, G. C., Wehrmeister, T. D., Izídio, G. S. (2008) Integrating the open field, elevated plus maze and light/dark box to assess different types of emotional behaviors in one single trial. Behav. Brain. Res. 193, 277288.

    • Search Google Scholar
    • Export Citation
  • 41.

    Saitoh, A., Makino, Y., Hashimoto, T., Yamada, M., Gotoh, L., Sugiyama, A., Ohashi, M., Tsukagoshi, M., Oka, J., Yamada, M. (2015) The voltage-gated sodium channel activator veratrine induces anxiogenic-like behaviors in rats. Behav. Brain. Res. 292, 316322.

    • Search Google Scholar
    • Export Citation
  • 42.

    Sarwar, R., Farooq, U., Naz, S. (2018) Isolation and Characterization of Two New Secondary Metabolites From Quercus incana and Their Antidepressant- and Anxiolytic-Like Potential. Front Pharmacol. 9, 298.

    • Search Google Scholar
    • Export Citation
  • 43.

    Scheinert, R. B., Haeri, M.H1, Lehmann, M. L., Herkenham, M. (2016) Therapeutic effects of stressprogrammed lymphocytes transferred to chronically stressed mice. Prog Neuropsychopharmacol. Biol. Psychiatry 70, 17.

    • Search Google Scholar
    • Export Citation
  • 44.

    Shastry, R., Ullal, S. D., Karkala, S., Rai, S., Gadgade, A. (2016) Anxiolytic activity of aqueous extract of Camellia sinensis in rats. Indian J. Pharmacol. 48, 681686.

    • Search Google Scholar
    • Export Citation
  • 45.

    Shimada, T., Matsumoto, K., Osanai, M., Matsuda, H., Terasawa, K., Watanabe, H. (1995) The modified light/dark transition test in mice: evaluation of classic and putative anxiolytic and anxiogenic drugs. Gen. Pharmacol. 26, 205210.

    • Search Google Scholar
    • Export Citation
  • 46.

    Sidor, M. M., Rilett, K., Foster, J. A. (2010) Validation of an automated system for measuring anxietyrelated behaviours in the elevated plus maze. J. Neurosci. Methods 188, 713.

    • Search Google Scholar
    • Export Citation
  • 47.

    Steimer, T. (2002) The biology of fear- and anxiety-related behaviors. Dialogues. Clin. Neurosci. 4, 231249.

  • 48.

    Takao, K., Miyakawa, T. (2006) Light/dark transition test for mice. J. Vis. Exp. 13, 104.

  • 49.

    Young, R., Johnson, D. N. (1991) A fully automated light/dark apparatus useful for comparing anxiolytic agents. Pharmacol. Biochem. Behav. 40, 739743.

    • Search Google Scholar
    • Export Citation
  • 50.

    Yu, C., Mei, X. T., Zheng, Y. P., Xu, D. H. (2015) Taurine zinc solid dispersions protect against cold-restraint stress-induced gastric ulceration by upregulating HSP70 and exerting an anxiolytic effect. Eur. J. Pharmacol. 762, 6371.

    • Search Google Scholar
    • Export Citation

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

Acta Biologica Hungarica
P.O. Box 35
H-8237 Tihany, Hungary
Phone: (36 87) 448 244 ext. 103
Fax: (36 87) 448 006
E-mail: elekes@tres.blki.hu

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture
  • Biology and Environmental Sciences
  • Elsevier BIOBASE
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Acta Biologica Hungarica
Language English
Size  
Year of
Foundation
1950
Publication
Programme
changed title
Volumes
per Year
 
Issues
per Year
 
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)