View More View Less
  • 1 College of Life Sciences, Shaanxi Normal University, Xi’an, China
  • | 2 Institute of Pure and Applied Biology Bahauddin Zakariya University, Multan, Pakistan
Restricted access

Drought is one the critical abiotic factors that reduces the germination, growth and yield of crops. Therefore the present project was designed with the objective to screen the best drought tolerant hybrid of Vigna radiata. Genetic variations for drought tolerance among these hybrids were assessed by simple and efficient technique. Seven hybrids of V. radiata (9801, 7002, 9706, 08003, 07007, 97012 and 08007) were used for screening against three levels of drought stress (zero, control), 5% (–0.05 MPa) and 10% (–0.1 MPa) induced by Polyethyleneglycol (PEG6000). A higher proline content was observed in 07007 (6.10 μg/g fresh weight) as compared to all other hybrids. Treated seedlings of each hybrid were compared with their respective control to evaluate the differences in their growth under drought. Different parameters such as percentage of germination, germination stress tolerance index (GSI), shoot and root weights (bath fresh and dry) and lengths, root length stress index (RLSI), dry matter stress index (DMSI) and plant height stress index (PHSI) showed considerable variations. Germination percentage, shoot weights, PHSI and DMSI decreased in all hybrids along with the increase of PEG induced drought stress (5% and 10% PEG). In contrary, root weights and RLSI were increased under drought. Overall 07007 showed a better performance, and can therefore be classified as a drought tolerant hybrid.

  • 1.

    Abdoli, M., Saeidi, M. (2012) Effects of water deficiency stress during seed growth on yield and its components, germination and seedling growth parameters of some wheat cultivars. Intl. J. Agri. Crop Sci. 4, 11101118.

    • Search Google Scholar
    • Export Citation
  • 2.

    Agili, S., Nyende, B., Ngamau, K., Masinde, P. (2012) Selection, yield evaluation, drought tolerance indices of orange-flesh sweet potato (Ipomoea batatas Lam) Hybrid clone. Nutr. Food Sci. 2, 138.

    • Search Google Scholar
    • Export Citation
  • 3.

    Ahmad, S., Ahmad, R., Ashraf, M. Y., Ashraf, M., Waraich, E. A. (2009) Sunflower (Helianthus annuus L.) response to drought stress at germination and growth stages. Pak. J. Bot. 41, 647654.

    • Search Google Scholar
    • Export Citation
  • 4.

    Anupamaa, A., Bhugrab, S., Lallb, B., Chaudhury, S., Chugh, A. (2018) Assessing the correlation of genotypic and phenotypic responses of rice varieties under drought stress. Plant Physiol. Biochem. 127, 343354

    • Search Google Scholar
    • Export Citation
  • 5.

    Ashraf, M., Bokhari, H., Cristiti, S. N. (1992) Variation in osmotic adjustment of lentil (Lens culinaris Medic) in response to drought. Acta Bot. Neerl. 41, 5162.

    • Search Google Scholar
    • Export Citation
  • 6.

    Ashraf, M. Y., Akhtar, K., Hussain, F., Iqbal, J. (2006) Screening of different accessions of three potential grass species from Cholistan desert for salt tolerance. Pak. J. Bot. 38, 15891597.

    • Search Google Scholar
    • Export Citation
  • 7.

    Avramova, V., Abd-Elgawad, H., Zhang, Z., Fotschki, B., Casadevall, R., Vergauwen, L., Knapen, D., Taleisnik, E., Guisez, Y., Asard, H., Beemster, G. T. S. (2015) Drought induces distinct growth response, protection, and recovery mechanisms in the maize leaf growth zone. Plant Physiol. 169, 13821396.

    • Search Google Scholar
    • Export Citation
  • 8.

    Badiane, F. A., Diouf, D., Sane, D., Diouf, O., Goudiaby, V., Diallo, N. (2004) Screening cowpea [Vigna unguiculata (L.) Walp.] varieties by inducing water deficit and RAPD analyses. Afr. J. Biotechnol. 3, 174178.

    • Search Google Scholar
    • Export Citation
  • 9.

    Bashir, N., Mahmood, S., Zafar, Z. A., Rasul, S., Manzoor, H., Athar, H. R. (2016) Is drought tolerance in maize (Zea mays L.) cultivars at the juvenile stage maintained at the reproductive stage? Pak. J. Bot. 48, 13851392.

    • Search Google Scholar
    • Export Citation
  • 10.

    Bates, L. S., Waldrem, R. P., Teare, I. D. (1972) Report of determination of free proline in water stress studies. Plant and Soil 39, 205208.

    • Search Google Scholar
    • Export Citation
  • 11.

    Batool, N., Ilyas, N., Noor, T., Saeed, M., Mazhar, R., Bibi, F., Shahzad, A. (2014) Evaluation of drought stress effects on germination and seedling growth of Zea mays L. Int. J. Biosci. 5, 203209.

    • Search Google Scholar
    • Export Citation
  • 12.

    Bibi, A., Sadaqat, H. A., Tahir, M. H. N., Akram, H. M. (2012) Screening of Sorghum bicolor Var Moench for drought tolerance at seedling stage in polyethyleneglycol. J. Animal Plant Sci. 22, 671678.

    • Search Google Scholar
    • Export Citation
  • 13.

    Cavallaroa, V., Barbera, A. C., Maucieri, C., Gimmaa, G., Scalisi, C., Patanea, C. (2016) Evaluation of variability to drought and saline stress through the germination of different ecotypes of carob (Ceratonia siliqua L.) using a hydrotime model. Ecol. Eng. 95, 557566.

    • Search Google Scholar
    • Export Citation
  • 14.

    Cooper, M., Gho, C., Leafgren, R., Tang, T., Messina, C. (2014) Breeding drought-tolerant maize hybrids for the US corn-belt: discovery to product. J. Exp. Bot. 65, 61916204.

    • Search Google Scholar
    • Export Citation
  • 15.

    Delachiave, M. E. A., De Pinho, S. Z. (2003) Germination of Seena occidentalis link: seeds at different osmotic potential levels. Braz. Arch. Techno. 46, 163166.

    • Search Google Scholar
    • Export Citation
  • 16.

    Ebrahimi, D. M., Shirmohammadi, E. (2017) Effectiveness of plant growth promoting rhizobacteria on Bromus tomentellus Boiss seed germination, growth and nutrients uptake under drought stress. S. Afr. J. Bot. 113, 1118.

    • Search Google Scholar
    • Export Citation
  • 17.

    Fahad, S., Bajwa, A. A., Nazir, U., Anjum, S. A., Farooq, A., Zohaib, A., Sadia, S., Nasim, W., Adkins, S., Saud, S., Ihsan, M. Z., Alharby, H., Wu, C., Wang, D., Huang, J. (2017) Crop Production under Drought and Heat Stress: Plant Responses and Management Options. Front. Plant Sci. 8, 1147.

    • Search Google Scholar
    • Export Citation
  • 18.

    Hohl, M., Peter, S. (1991) Water relation of growing maize coleoptiles. Comparison between mannitol and polyethylene glycol 6000 as external osmotica for adjusting turgor pressure. Plant Physiol. 95, 716722.

    • Search Google Scholar
    • Export Citation
  • 19.

    Hu, F. D., Jones, R. J. (2004) Effects of plant extracts of Bothriochloa pertusa and Urochloa mosambicensis on seed germination and seedling growth of Stylosanthes hamata cv. Verano and Stylosanthes scabra cv. Seca. Aust. J. Agric. Res. 48, 12571264.

    • Search Google Scholar
    • Export Citation
  • 20.

    Kalefetoglu, T., Macar, T., Turan, O., Ekmekci, Y. (2009) Effect of water stress deficit induced by PEG and Nacl on Chickpea (Cicer arietinum L.) cultivars and lines at early seedling stage. G. U. J. Sci. 22, 514.

    • Search Google Scholar
    • Export Citation
  • 21.

    Kaya, M. D., Okcu, G., Atak, M., Cikili, Y., Kolsarici, O. (2006) Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). Eur. J. Agron. 24, 291295.

    • Search Google Scholar
    • Export Citation
  • 22.

    Khayatnezhad, M., Gholamin, R., Jamaatie-Somarin, S. H., Zabihi-Mahmoodabad, R. (2010) Effects of PEG stress on corn cultivars (Zea mays L.) at germination stage. World Appl. Sci. J. 11, 504506.

    • Search Google Scholar
    • Export Citation
  • 23.

    Kulkarni, M., Deshpande, U. (2007) In vitro screening of tomato genotypes for drought resistance using polyethylene glycol. Afr. J. Biotechnol. 6, 691696.

    • Search Google Scholar
    • Export Citation
  • 24.

    Lafitte, H. R., Yongsheng, G., Yan, S., Li, Z. K. (2007) Whole plant responses, key processes, and adaptation to drought stress: the case of rice. J. Exp. Bot. 58, 169175.

    • Search Google Scholar
    • Export Citation
  • 25.

    Macart, K., Turan, Y., Ekmekci, T. (2009) Effect of water deficit induced by PEG and NaCl on chickpea (Cicer arietinum L.) cultivar and lines at early seedling stage. Gazi Uni. J. Sci. 22, 514.

    • Search Google Scholar
    • Export Citation
  • 26.

    Naidu, T. C. M., Raju, N., Narayanan, A. (2001) Screening of drought tolerance in green gram (Vigna radiata L. Wilczek) genotypes under receding soil moisture. Indian J. plant physiol. 6, 197201.

    • Search Google Scholar
    • Export Citation
  • 27.

    Naseer, S., Nisar, A., Ashraf, M. (2001) Effect of Salt Stress on Germination and Seedling Growth of Barley (Hordeum vulgare L.). Pak. J. Biol. Sci. 4, 359360.

    • Search Google Scholar
    • Export Citation
  • 28.

    Petrovic, G., Jovicic, D., Nikolic, Z., Tamindzic, G., Ignjatov, M., Milosevic, D., Milosevic, B. (2016) Comparative study of drought and salt stress effects on germination and seedling growth of pea. Genetika 48, 373381.

    • Search Google Scholar
    • Export Citation
  • 29.

    Rajendran, R. A., Muthiah, A., Manickam, P., Shanmugasundaram, A., John, J. (2011) Indices of drought tolerance in sorghum (Sorghum bicolor L. Moench) genotypes at early stages of plant growth. Res. J. Agric. Biol. Sci. 7, 4246.

    • Search Google Scholar
    • Export Citation
  • 30.

    Rauf, S., Sadaqat, H. A., Khan, I. A. (2008) Effect of moisture regimes on combining ability variations of seedling traits in sunflower (Helianthus annuus L.). Can. J. Plant Sci. 88, 323329.

    • Search Google Scholar
    • Export Citation
  • 31.

    Rehman, A., Jingdong, L., Shahzad, B., Chandio, A. A., Hussain, I., Nabie, G., Iqbal, M. S. (2015) Economic perspectives of major field crops of Pakistan: An empirical study. Pacific Science Review B: Humanities and Social Sciences 1, 145158.

    • Search Google Scholar
    • Export Citation
  • 32.

    Ribeiro, N. M., Torres, B. A., Ramos, S. K., Santos, P. H., Simoes, C. T., Monquero, P. A. (2018) Differential susceptibility of morning glory (Ipomoea and Merremia) species to residual herbicides and the effect of drought periods on efficacy. Aust. J. Crop Sci. 12, 10901098.

    • Search Google Scholar
    • Export Citation
  • 33.

    Richards, R. A. (1978) Variation between and within species of rapeseed (Brassica campestris and B. napus) in response to drought stress: Physiological and physicochemical characters. Aust. J. Agric. Res. 29, 491501.

    • Search Google Scholar
    • Export Citation
  • 34.

    Shaheen, R., Hood-Nowotny, R. C. (2005) Effect of drought and salinity on carbon isotope discrimination in wheat cultivars. Plant Sci. 168, 901909.

    • Search Google Scholar
    • Export Citation
  • 35.

    Shamim, F., Saqlan, S. M., Athar, H. R., Waheed, A. (2014) Screening and selection of tomato genotypes/cultivars for drought tolerance using multivariate analysis. Pak. J. Bot. 46, 11651178.

    • Search Google Scholar
    • Export Citation
  • 36.

    Shu-han, Z., Xue-feng, X., Ye-min, S., Jun-lian, Z., Chao-zhou, L. (2018) Influence of drought hardening on the resistance physiology of potato seedlings under drought stress. J. Integr. Agric. 17, 336347.

    • Search Google Scholar
    • Export Citation
  • 37.

    Smykal, P., Aubert, G., Burstin, J., Coyn, C. J., Ellis, N. T. H., Flavell, A. J., Ford, R., Hybl, M., Macas, J., Neumann, P., Mc-Phee, K. E., Redden, R. J., Rubiales, D., Weller, J. L., Warkentin, T. L. (2012) Pea (Pisum sativum L.) in the Genomic Era. Agronomy 2, 74115.

    • Search Google Scholar
    • Export Citation
  • 38.

    Snedecor, G. W., Cochran, W. G. (1980) Statistical methods. 7th edition, Iowa State University Press, Ames, Iowa.

  • 39.

    Soltani, A., Gholipoor, M., Zeinali, E. (2006) Seed reserve utilization and seedling growth of wheat as affected by drought and salinity. Environ. Exp. Bot. 55, 195200.

    • Search Google Scholar
    • Export Citation
  • 40.

    Verslues, P. E., Agarwal, M., Katiyar-Agarwal, S., Zhu, J., Zhu, J. K. (2006) Methods and concepts in quantifying resistance to drought, salt and freezing, abiotic stresses that affect plant water status. Plant J. 45, 523539.

    • Search Google Scholar
    • Export Citation

Editorial Board

    1. Csányi, Vilmos (Göd)
    1. Dudits, Dénes (Szeged)
    1. Falus, András (Budapest)
    1. Fischer, Ernő (Pécs)
    1. Gábriel, Róbert (Pécs)
    1. Gulya, Károly (Szeged)
    1. Gulyás, Balázs (Stockholm)
    1. Hajós, Ferenc (Budapest)
    1. Hámori, József (Budapest)
    1. Heszky, László (Gödöllő)
    1. Hideg, Éva (Szeged)
    1. E. Ito (Sanuki)
    1. Janda, Tibor (Martonvásár)
    1. Kavanaugh, Michael P. (Missoula)
    1. Kása, Péter (Szeged)
    1. Klein, Éva (Stockholm)
    1. Kovács, János (Budapest)
    1. Brigitte Mauch-Mani (Neuchâtel)
    1. Nässel, Dick R. (Stockholm)
    1. Nemcsók, János (Szeged)
    1. Péczely, Péter (Gödöllő)
    1. Roberts, D. F. (Newcastle-upon-Tyne)
    1. Sakharov, Dimitri A. (Moscow)
    1. Singh, Meharvan (Fort Worth)
    1. Sipiczky, Mátyás (Debrecen)
    1. Szeberényi, József (Pécs)
    1. Székely, György (Debrecen)
    1. Tari, Irma (Szeged)
    1. Vágvölgyi, Csaba (Szeged),
    1. L. Zaborszky (Newark)

 

Acta Biologica Hungarica
P.O. Box 35
H-8237 Tihany, Hungary
Phone: (36 87) 448 244 ext. 103
Fax: (36 87) 448 006
E-mail: elekes@tres.blki.hu

Indexing and Abstracting Services:

  • Biological Abstracts
  • BIOSIS Previews
  • CAB Abstracts
  • Chemical Abstracts
  • Current Contents: Agriculture
  • Biology and Environmental Sciences
  • Elsevier BIOBASE
  • Global Health
  • Index Medicus
  • Index Veterinarius
  • Medline
  • Referativnyi Zhurnal
  • Science Citation Index
  • Science Citation Index Expanded (SciSearch)
  • SCOPUS
  • The ISI Alerting Services
  • Zoological Abstracts

 

Acta Biologica Hungarica
Language English
Size  
Year of
Foundation
1950
Publication
Programme
changed title
Volumes
per Year
 
Issues
per Year
 
Founder Magyar Tudományos Akadémia
Founder's
Address
H-1051 Budapest, Hungary, Széchenyi István tér 9.
Publisher Akadémiai Kiadó
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0236-5383 (Print)
ISSN 1588-256X (Online)